Streptococcus phage CHPC929

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Brussowvirus; unclassified Brussowvirus

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8FAW3|A0A3G8FAW3_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC929 OX=2365050 GN=CHPC929_0037 PE=4 SV=1
MM1 pKa = 7.13VNWVDD6 pKa = 4.56ANGNDD11 pKa = 4.18LPDD14 pKa = 4.33GADD17 pKa = 3.29QEE19 pKa = 5.13FKK21 pKa = 11.28AGMFFSFSDD30 pKa = 3.87DD31 pKa = 3.14EE32 pKa = 4.64TNIVDD37 pKa = 3.86TGNGGYY43 pKa = 10.4YY44 pKa = 9.73GGYY47 pKa = 9.69YY48 pKa = 9.72YY49 pKa = 10.64RR50 pKa = 11.84QFEE53 pKa = 4.29FGQFGPVWLSCWNKK67 pKa = 10.66DD68 pKa = 3.89DD69 pKa = 5.14LVNYY73 pKa = 7.38YY74 pKa = 8.26QQ75 pKa = 4.22

Molecular weight: 8.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8F885|A0A3G8F885_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC929 OX=2365050 GN=CHPC929_0016 PE=4 SV=1
MM1 pKa = 7.03QVKK4 pKa = 9.86EE5 pKa = 3.76YY6 pKa = 10.9ALYY9 pKa = 10.48KK10 pKa = 9.35GWEE13 pKa = 4.01RR14 pKa = 11.84KK15 pKa = 9.38ARR17 pKa = 11.84LLNRR21 pKa = 11.84RR22 pKa = 11.84KK23 pKa = 8.62TSIRR27 pKa = 11.84TKK29 pKa = 9.46NTEE32 pKa = 3.73EE33 pKa = 4.25GG34 pKa = 3.49

Molecular weight: 4.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

12648

34

1517

218.1

24.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.187 ± 0.882

0.735 ± 0.149

6.159 ± 0.398

6.641 ± 0.698

4.206 ± 0.171

6.879 ± 0.471

1.265 ± 0.174

6.863 ± 0.371

7.93 ± 0.575

7.535 ± 0.287

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.332 ± 0.262

6.009 ± 0.337

2.791 ± 0.198

3.819 ± 0.245

4.111 ± 0.356

6.95 ± 0.607

6.697 ± 0.413

6.491 ± 0.223

1.336 ± 0.165

4.064 ± 0.41
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski