Leptosphaeria biglobosa mitovirus 1
Average proteome isoelectric point is 8.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2R4SUF2|A0A2R4SUF2_9VIRU RNA-dependent RNA polymerase OS=Leptosphaeria biglobosa mitovirus 1 OX=2163916 PE=4 SV=1
MM1 pKa = 7.41 SLLFKK6 pKa = 10.66 ILITMKK12 pKa = 10.39 QNNYY16 pKa = 10.3 LILEE20 pKa = 4.41 RR21 pKa = 11.84 LLKK24 pKa = 10.73 SLFKK28 pKa = 10.01 IDD30 pKa = 3.74 CFAIVHH36 pKa = 5.81 QYY38 pKa = 11.26 LNYY41 pKa = 8.75 IDD43 pKa = 5.25 RR44 pKa = 11.84 MRR46 pKa = 11.84 EE47 pKa = 4.05 KK48 pKa = 10.86 NGLAYY53 pKa = 9.21 TIKK56 pKa = 10.38 HH57 pKa = 4.93 MKK59 pKa = 8.74 VVKK62 pKa = 10.05 LHH64 pKa = 5.08 ITRR67 pKa = 11.84 FICNKK72 pKa = 8.95 PLKK75 pKa = 10.46 SNSNYY80 pKa = 9.93 VSLDD84 pKa = 2.75 KK85 pKa = 11.03 DD86 pKa = 3.75 YY87 pKa = 11.08 FPSRR91 pKa = 11.84 FLYY94 pKa = 10.46 LKK96 pKa = 9.88 RR97 pKa = 11.84 FCKK100 pKa = 10.0 TNPDD104 pKa = 3.4 LVLTLLSYY112 pKa = 10.33 TRR114 pKa = 11.84 ALTPNKK120 pKa = 10.2 RR121 pKa = 11.84 EE122 pKa = 3.95 TNARR126 pKa = 11.84 NVDD129 pKa = 2.94 ISSITNPYY137 pKa = 8.86 KK138 pKa = 10.92 GKK140 pKa = 10.48 DD141 pKa = 3.3 YY142 pKa = 10.11 TIPIWFIKK150 pKa = 10.54 DD151 pKa = 4.08 FINHH155 pKa = 6.74 HH156 pKa = 6.1 GLSLSKK162 pKa = 10.11 PVYY165 pKa = 10.45 SNSDD169 pKa = 3.0 HH170 pKa = 6.18 YY171 pKa = 11.57 LSIKK175 pKa = 10.22 GSPNGKK181 pKa = 9.87 SSISSLWSVASHH193 pKa = 6.7 NEE195 pKa = 3.45 NTLEE199 pKa = 4.19 YY200 pKa = 9.92 IRR202 pKa = 11.84 TITGEE207 pKa = 3.92 FFKK210 pKa = 10.77 EE211 pKa = 3.35 ISNYY215 pKa = 5.58 YY216 pKa = 8.51 TKK218 pKa = 10.44 IVLDD222 pKa = 4.01 YY223 pKa = 11.53 SHH225 pKa = 7.4 LIDD228 pKa = 4.03 NNKK231 pKa = 9.95 KK232 pKa = 9.56 ILGKK236 pKa = 10.4 LSVVHH241 pKa = 7.19 DD242 pKa = 4.35 PEE244 pKa = 4.86 LKK246 pKa = 9.79 EE247 pKa = 4.09 RR248 pKa = 11.84 VIAMVDD254 pKa = 3.27 YY255 pKa = 7.64 TTQFTLKK262 pKa = 10.0 PIHH265 pKa = 5.96 NQLLKK270 pKa = 10.89 LLSKK274 pKa = 10.16 FDD276 pKa = 3.93 CDD278 pKa = 3.34 RR279 pKa = 11.84 TFTQDD284 pKa = 3.97 PFHH287 pKa = 6.72 NWQCNSEE294 pKa = 4.28 NFHH297 pKa = 7.78 SLDD300 pKa = 3.55 LSAATDD306 pKa = 3.92 RR307 pKa = 11.84 FPIDD311 pKa = 3.68 LQEE314 pKa = 4.45 KK315 pKa = 8.53 LLSYY319 pKa = 10.45 IYY321 pKa = 10.03 DD322 pKa = 3.92 DD323 pKa = 4.77 KK324 pKa = 11.82 DD325 pKa = 3.49 FAKK328 pKa = 10.01 SWRR331 pKa = 11.84 NLLVDD336 pKa = 3.83 RR337 pKa = 11.84 DD338 pKa = 4.51 FIYY341 pKa = 9.8 EE342 pKa = 4.07 QKK344 pKa = 10.5 SIRR347 pKa = 11.84 YY348 pKa = 8.98 SVGQPMGAYY357 pKa = 8.28 TSWAAFTLTHH367 pKa = 6.32 HH368 pKa = 7.16 LVVQWAAKK376 pKa = 10.32 LCGLDD381 pKa = 4.34 HH382 pKa = 6.8 FNQYY386 pKa = 10.4 IILGDD391 pKa = 4.36 DD392 pKa = 3.31 IVIKK396 pKa = 10.53 NNKK399 pKa = 7.97 VANKK403 pKa = 9.52 YY404 pKa = 7.41 ITIMTRR410 pKa = 11.84 LGVDD414 pKa = 3.41 ISQPKK419 pKa = 7.67 THH421 pKa = 6.23 VSNDD425 pKa = 3.06 TYY427 pKa = 11.38 EE428 pKa = 4.02 FAKK431 pKa = 10.35 RR432 pKa = 11.84 WIQKK436 pKa = 9.22 GRR438 pKa = 11.84 EE439 pKa = 3.81 ISGLSLKK446 pKa = 10.9 GILNNFKK453 pKa = 10.29 NKK455 pKa = 9.71 HH456 pKa = 4.16 VVYY459 pKa = 10.13 MNIFNYY465 pKa = 9.12 YY466 pKa = 9.61 LRR468 pKa = 11.84 RR469 pKa = 11.84 PHH471 pKa = 8.32 LDD473 pKa = 2.95 IDD475 pKa = 4.12 LLNLIGNLYY484 pKa = 10.41 EE485 pKa = 4.76 GIRR488 pKa = 11.84 VSNRR492 pKa = 11.84 IKK494 pKa = 10.83 SKK496 pKa = 9.08 NTIIKK501 pKa = 10.03 LLYY504 pKa = 10.03 DD505 pKa = 3.53 FHH507 pKa = 8.45 HH508 pKa = 7.19 SIRR511 pKa = 11.84 FSFNLLSYY519 pKa = 10.93 EE520 pKa = 5.41 DD521 pKa = 3.2 IRR523 pKa = 11.84 KK524 pKa = 9.33 YY525 pKa = 11.07 LNNKK529 pKa = 8.76 IVIEE533 pKa = 4.25 EE534 pKa = 4.08 FNVWPEE540 pKa = 3.5 SLIPYY545 pKa = 9.33 KK546 pKa = 10.3 IRR548 pKa = 11.84 EE549 pKa = 4.01 ILSYY553 pKa = 11.24 GMVPQAEE560 pKa = 4.25 SLKK563 pKa = 10.06 TNISKK568 pKa = 10.26 QMSILRR574 pKa = 11.84 EE575 pKa = 4.21 SKK577 pKa = 10.58 DD578 pKa = 3.23 IPIDD582 pKa = 4.32 LLVDD586 pKa = 3.72 WPLTYY591 pKa = 10.63 GFKK594 pKa = 10.7 NHH596 pKa = 6.61 IEE598 pKa = 4.13 SMTNLIEE605 pKa = 4.43 GFVNKK610 pKa = 9.68 TSQLLDD616 pKa = 4.04 IISHH620 pKa = 6.38 LRR622 pKa = 11.84 LNNLDD627 pKa = 4.49 GIMKK631 pKa = 10.22 SRR633 pKa = 11.84 LNNQDD638 pKa = 4.76 LLLLDD643 pKa = 5.01 KK644 pKa = 10.6 LWKK647 pKa = 10.4 DD648 pKa = 3.23 SFSRR652 pKa = 11.84 YY653 pKa = 9.06 ILALKK658 pKa = 8.49 TEE660 pKa = 4.33 KK661 pKa = 10.95 EE662 pKa = 4.24 EE663 pKa = 4.11 ILNSPEE669 pKa = 5.0 DD670 pKa = 3.65 KK671 pKa = 10.89 PKK673 pKa = 11.21 DD674 pKa = 3.68 LVSHH678 pKa = 6.37 IWSMAWASTPVVYY691 pKa = 8.69 KK692 pKa = 10.26 TSFVGLKK699 pKa = 10.34 SFEE702 pKa = 4.42 LYY704 pKa = 10.41 LYY706 pKa = 10.74 EE707 pKa = 5.91 SFDD710 pKa = 4.45 GLNTDD715 pKa = 3.74 LDD717 pKa = 4.12 YY718 pKa = 11.64 LRR720 pKa = 11.84 LEE722 pKa = 4.26 TRR724 pKa = 11.84 VDD726 pKa = 3.46 EE727 pKa = 4.62 SSTNIQDD734 pKa = 4.31 RR735 pKa = 11.84 IHH737 pKa = 7.03 SFVYY741 pKa = 10.51 DD742 pKa = 3.61 SIKK745 pKa = 10.76 NNKK748 pKa = 8.67 HH749 pKa = 5.71 INWDD753 pKa = 3.51 EE754 pKa = 4.24 FKK756 pKa = 11.19
Molecular weight: 88.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.481
IPC2_protein 8.478
IPC_protein 8.331
Toseland 8.96
ProMoST 8.887
Dawson 9.297
Bjellqvist 9.092
Wikipedia 9.487
Rodwell 9.487
Grimsley 9.385
Solomon 9.326
Lehninger 9.282
Nozaki 9.107
DTASelect 9.033
Thurlkill 9.136
EMBOSS 9.428
Sillero 9.282
Patrickios 4.431
IPC_peptide 9.326
IPC2_peptide 7.658
IPC2.peptide.svr19 7.633
Protein with the highest isoelectric point:
>tr|A0A2R4SUF2|A0A2R4SUF2_9VIRU RNA-dependent RNA polymerase OS=Leptosphaeria biglobosa mitovirus 1 OX=2163916 PE=4 SV=1
MM1 pKa = 7.41 SLLFKK6 pKa = 10.66 ILITMKK12 pKa = 10.39 QNNYY16 pKa = 10.3 LILEE20 pKa = 4.41 RR21 pKa = 11.84 LLKK24 pKa = 10.73 SLFKK28 pKa = 10.01 IDD30 pKa = 3.74 CFAIVHH36 pKa = 5.81 QYY38 pKa = 11.26 LNYY41 pKa = 8.75 IDD43 pKa = 5.25 RR44 pKa = 11.84 MRR46 pKa = 11.84 EE47 pKa = 4.05 KK48 pKa = 10.86 NGLAYY53 pKa = 9.21 TIKK56 pKa = 10.38 HH57 pKa = 4.93 MKK59 pKa = 8.74 VVKK62 pKa = 10.05 LHH64 pKa = 5.08 ITRR67 pKa = 11.84 FICNKK72 pKa = 8.95 PLKK75 pKa = 10.46 SNSNYY80 pKa = 9.93 VSLDD84 pKa = 2.75 KK85 pKa = 11.03 DD86 pKa = 3.75 YY87 pKa = 11.08 FPSRR91 pKa = 11.84 FLYY94 pKa = 10.46 LKK96 pKa = 9.88 RR97 pKa = 11.84 FCKK100 pKa = 10.0 TNPDD104 pKa = 3.4 LVLTLLSYY112 pKa = 10.33 TRR114 pKa = 11.84 ALTPNKK120 pKa = 10.2 RR121 pKa = 11.84 EE122 pKa = 3.95 TNARR126 pKa = 11.84 NVDD129 pKa = 2.94 ISSITNPYY137 pKa = 8.86 KK138 pKa = 10.92 GKK140 pKa = 10.48 DD141 pKa = 3.3 YY142 pKa = 10.11 TIPIWFIKK150 pKa = 10.54 DD151 pKa = 4.08 FINHH155 pKa = 6.74 HH156 pKa = 6.1 GLSLSKK162 pKa = 10.11 PVYY165 pKa = 10.45 SNSDD169 pKa = 3.0 HH170 pKa = 6.18 YY171 pKa = 11.57 LSIKK175 pKa = 10.22 GSPNGKK181 pKa = 9.87 SSISSLWSVASHH193 pKa = 6.7 NEE195 pKa = 3.45 NTLEE199 pKa = 4.19 YY200 pKa = 9.92 IRR202 pKa = 11.84 TITGEE207 pKa = 3.92 FFKK210 pKa = 10.77 EE211 pKa = 3.35 ISNYY215 pKa = 5.58 YY216 pKa = 8.51 TKK218 pKa = 10.44 IVLDD222 pKa = 4.01 YY223 pKa = 11.53 SHH225 pKa = 7.4 LIDD228 pKa = 4.03 NNKK231 pKa = 9.95 KK232 pKa = 9.56 ILGKK236 pKa = 10.4 LSVVHH241 pKa = 7.19 DD242 pKa = 4.35 PEE244 pKa = 4.86 LKK246 pKa = 9.79 EE247 pKa = 4.09 RR248 pKa = 11.84 VIAMVDD254 pKa = 3.27 YY255 pKa = 7.64 TTQFTLKK262 pKa = 10.0 PIHH265 pKa = 5.96 NQLLKK270 pKa = 10.89 LLSKK274 pKa = 10.16 FDD276 pKa = 3.93 CDD278 pKa = 3.34 RR279 pKa = 11.84 TFTQDD284 pKa = 3.97 PFHH287 pKa = 6.72 NWQCNSEE294 pKa = 4.28 NFHH297 pKa = 7.78 SLDD300 pKa = 3.55 LSAATDD306 pKa = 3.92 RR307 pKa = 11.84 FPIDD311 pKa = 3.68 LQEE314 pKa = 4.45 KK315 pKa = 8.53 LLSYY319 pKa = 10.45 IYY321 pKa = 10.03 DD322 pKa = 3.92 DD323 pKa = 4.77 KK324 pKa = 11.82 DD325 pKa = 3.49 FAKK328 pKa = 10.01 SWRR331 pKa = 11.84 NLLVDD336 pKa = 3.83 RR337 pKa = 11.84 DD338 pKa = 4.51 FIYY341 pKa = 9.8 EE342 pKa = 4.07 QKK344 pKa = 10.5 SIRR347 pKa = 11.84 YY348 pKa = 8.98 SVGQPMGAYY357 pKa = 8.28 TSWAAFTLTHH367 pKa = 6.32 HH368 pKa = 7.16 LVVQWAAKK376 pKa = 10.32 LCGLDD381 pKa = 4.34 HH382 pKa = 6.8 FNQYY386 pKa = 10.4 IILGDD391 pKa = 4.36 DD392 pKa = 3.31 IVIKK396 pKa = 10.53 NNKK399 pKa = 7.97 VANKK403 pKa = 9.52 YY404 pKa = 7.41 ITIMTRR410 pKa = 11.84 LGVDD414 pKa = 3.41 ISQPKK419 pKa = 7.67 THH421 pKa = 6.23 VSNDD425 pKa = 3.06 TYY427 pKa = 11.38 EE428 pKa = 4.02 FAKK431 pKa = 10.35 RR432 pKa = 11.84 WIQKK436 pKa = 9.22 GRR438 pKa = 11.84 EE439 pKa = 3.81 ISGLSLKK446 pKa = 10.9 GILNNFKK453 pKa = 10.29 NKK455 pKa = 9.71 HH456 pKa = 4.16 VVYY459 pKa = 10.13 MNIFNYY465 pKa = 9.12 YY466 pKa = 9.61 LRR468 pKa = 11.84 RR469 pKa = 11.84 PHH471 pKa = 8.32 LDD473 pKa = 2.95 IDD475 pKa = 4.12 LLNLIGNLYY484 pKa = 10.41 EE485 pKa = 4.76 GIRR488 pKa = 11.84 VSNRR492 pKa = 11.84 IKK494 pKa = 10.83 SKK496 pKa = 9.08 NTIIKK501 pKa = 10.03 LLYY504 pKa = 10.03 DD505 pKa = 3.53 FHH507 pKa = 8.45 HH508 pKa = 7.19 SIRR511 pKa = 11.84 FSFNLLSYY519 pKa = 10.93 EE520 pKa = 5.41 DD521 pKa = 3.2 IRR523 pKa = 11.84 KK524 pKa = 9.33 YY525 pKa = 11.07 LNNKK529 pKa = 8.76 IVIEE533 pKa = 4.25 EE534 pKa = 4.08 FNVWPEE540 pKa = 3.5 SLIPYY545 pKa = 9.33 KK546 pKa = 10.3 IRR548 pKa = 11.84 EE549 pKa = 4.01 ILSYY553 pKa = 11.24 GMVPQAEE560 pKa = 4.25 SLKK563 pKa = 10.06 TNISKK568 pKa = 10.26 QMSILRR574 pKa = 11.84 EE575 pKa = 4.21 SKK577 pKa = 10.58 DD578 pKa = 3.23 IPIDD582 pKa = 4.32 LLVDD586 pKa = 3.72 WPLTYY591 pKa = 10.63 GFKK594 pKa = 10.7 NHH596 pKa = 6.61 IEE598 pKa = 4.13 SMTNLIEE605 pKa = 4.43 GFVNKK610 pKa = 9.68 TSQLLDD616 pKa = 4.04 IISHH620 pKa = 6.38 LRR622 pKa = 11.84 LNNLDD627 pKa = 4.49 GIMKK631 pKa = 10.22 SRR633 pKa = 11.84 LNNQDD638 pKa = 4.76 LLLLDD643 pKa = 5.01 KK644 pKa = 10.6 LWKK647 pKa = 10.4 DD648 pKa = 3.23 SFSRR652 pKa = 11.84 YY653 pKa = 9.06 ILALKK658 pKa = 8.49 TEE660 pKa = 4.33 KK661 pKa = 10.95 EE662 pKa = 4.24 EE663 pKa = 4.11 ILNSPEE669 pKa = 5.0 DD670 pKa = 3.65 KK671 pKa = 10.89 PKK673 pKa = 11.21 DD674 pKa = 3.68 LVSHH678 pKa = 6.37 IWSMAWASTPVVYY691 pKa = 8.69 KK692 pKa = 10.26 TSFVGLKK699 pKa = 10.34 SFEE702 pKa = 4.42 LYY704 pKa = 10.41 LYY706 pKa = 10.74 EE707 pKa = 5.91 SFDD710 pKa = 4.45 GLNTDD715 pKa = 3.74 LDD717 pKa = 4.12 YY718 pKa = 11.64 LRR720 pKa = 11.84 LEE722 pKa = 4.26 TRR724 pKa = 11.84 VDD726 pKa = 3.46 EE727 pKa = 4.62 SSTNIQDD734 pKa = 4.31 RR735 pKa = 11.84 IHH737 pKa = 7.03 SFVYY741 pKa = 10.51 DD742 pKa = 3.61 SIKK745 pKa = 10.76 NNKK748 pKa = 8.67 HH749 pKa = 5.71 INWDD753 pKa = 3.51 EE754 pKa = 4.24 FKK756 pKa = 11.19
Molecular weight: 88.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.481
IPC2_protein 8.478
IPC_protein 8.331
Toseland 8.96
ProMoST 8.887
Dawson 9.297
Bjellqvist 9.092
Wikipedia 9.487
Rodwell 9.487
Grimsley 9.385
Solomon 9.326
Lehninger 9.282
Nozaki 9.107
DTASelect 9.033
Thurlkill 9.136
EMBOSS 9.428
Sillero 9.282
Patrickios 4.431
IPC_peptide 9.326
IPC2_peptide 7.658
IPC2.peptide.svr19 7.633
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
756
756
756
756.0
88.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.646 ± 0.0
0.794 ± 0.0
6.614 ± 0.0
4.365 ± 0.0
4.762 ± 0.0
3.042 ± 0.0
3.307 ± 0.0
9.127 ± 0.0
8.73 ± 0.0
12.169 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.72 ± 0.0
7.54 ± 0.0
3.042 ± 0.0
2.381 ± 0.0
4.365 ± 0.0
8.598 ± 0.0
5.026 ± 0.0
4.63 ± 0.0
1.72 ± 0.0
5.423 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here