Capybara microvirus Cap1_SP_217
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W867|A0A4P8W867_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_217 OX=2585412 PE=4 SV=1
MM1 pKa = 7.63 KK2 pKa = 10.63 LKK4 pKa = 8.76 TQKK7 pKa = 9.29 ATYY10 pKa = 9.13 FSCDD14 pKa = 2.92 GEE16 pKa = 4.59 FNDD19 pKa = 4.76 EE20 pKa = 4.36 ASMSQPNLAYY30 pKa = 10.33 SIKK33 pKa = 10.67 DD34 pKa = 3.28 LLEE37 pKa = 3.88 RR38 pKa = 11.84 AKK40 pKa = 10.72 RR41 pKa = 11.84 GIAPPDD47 pKa = 3.35 FTADD51 pKa = 3.3 YY52 pKa = 10.99 DD53 pKa = 4.58 GYY55 pKa = 9.41 TSPLTGDD62 pKa = 3.43 TGLDD66 pKa = 3.16 MDD68 pKa = 5.86 ADD70 pKa = 4.49 FDD72 pKa = 3.9 PTNRR76 pKa = 11.84 IGIDD80 pKa = 3.47 FDD82 pKa = 3.77 EE83 pKa = 4.8 LAEE86 pKa = 4.1 ISADD90 pKa = 2.94 GRR92 pKa = 11.84 KK93 pKa = 9.58 QMEE96 pKa = 4.22 EE97 pKa = 3.54 RR98 pKa = 11.84 RR99 pKa = 11.84 AEE101 pKa = 3.82 YY102 pKa = 10.35 LKK104 pKa = 10.77 RR105 pKa = 11.84 LEE107 pKa = 4.2 EE108 pKa = 3.95 QARR111 pKa = 11.84 AAAAPPADD119 pKa = 4.43 SPAEE123 pKa = 4.07 PPAAEE128 pKa = 3.96
Molecular weight: 14.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.442
IPC2_protein 4.279
IPC_protein 4.215
Toseland 4.024
ProMoST 4.317
Dawson 4.19
Bjellqvist 4.38
Wikipedia 4.088
Rodwell 4.05
Grimsley 3.935
Solomon 4.177
Lehninger 4.139
Nozaki 4.291
DTASelect 4.495
Thurlkill 4.05
EMBOSS 4.101
Sillero 4.329
Patrickios 3.948
IPC_peptide 4.177
IPC2_peptide 4.317
IPC2.peptide.svr19 4.257
Protein with the highest isoelectric point:
>tr|A0A4P8W867|A0A4P8W867_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_217 OX=2585412 PE=4 SV=1
MM1 pKa = 7.78 EE2 pKa = 4.99 SGKK5 pKa = 7.85 TQFFQKK11 pKa = 9.95 KK12 pKa = 8.14 KK13 pKa = 10.26 KK14 pKa = 9.81 KK15 pKa = 9.55 IKK17 pKa = 9.42 PSHH20 pKa = 5.3 TRR22 pKa = 11.84 AIIILLEE29 pKa = 4.27 NNNRR33 pKa = 11.84 PLTPNTKK40 pKa = 9.0 MAGILSTFLQGMFDD54 pKa = 3.89 RR55 pKa = 11.84 VSQNYY60 pKa = 10.39 NYY62 pKa = 9.13 TLQMDD67 pKa = 4.94 LANKK71 pKa = 9.45 EE72 pKa = 3.99 FAQNLEE78 pKa = 3.76 MWNLQNEE85 pKa = 4.57 YY86 pKa = 10.39 NSPKK90 pKa = 10.71 AQMEE94 pKa = 4.25 RR95 pKa = 11.84 LRR97 pKa = 11.84 EE98 pKa = 4.11 AGLNPNLVYY107 pKa = 10.86 GSGASTGNASSAPSYY122 pKa = 10.86 SRR124 pKa = 11.84 PNYY127 pKa = 9.73 QYY129 pKa = 11.37 QNVIKK134 pKa = 10.84 NLDD137 pKa = 3.59 LQSAVIEE144 pKa = 4.0 LLKK147 pKa = 11.0 SKK149 pKa = 11.15 EE150 pKa = 3.99 EE151 pKa = 4.11 LEE153 pKa = 5.07 LMRR156 pKa = 11.84 EE157 pKa = 4.07 QQKK160 pKa = 10.88 SIEE163 pKa = 4.35 SQTQLRR169 pKa = 11.84 QSQYY173 pKa = 10.03 NAQVLSNSMAQNQANAYY190 pKa = 8.58 YY191 pKa = 10.34 LYY193 pKa = 11.04 SLDD196 pKa = 3.52 SRR198 pKa = 11.84 YY199 pKa = 10.79 NGNPPADD206 pKa = 3.69 EE207 pKa = 4.26 VQGLLLNTHH216 pKa = 5.82 GRR218 pKa = 11.84 GTTDD222 pKa = 3.52 LDD224 pKa = 4.01 ALRR227 pKa = 11.84 KK228 pKa = 9.78 SPWYY232 pKa = 9.06 NQARR236 pKa = 11.84 LGQWTADD243 pKa = 3.37 KK244 pKa = 11.4 AMQQYY249 pKa = 11.29 LNLSEE254 pKa = 4.14 QNKK257 pKa = 8.05 MLQYY261 pKa = 11.09 QNLLNANQQQLFGNPSSMEE280 pKa = 3.83 GGQFIWALINALFGGKK296 pKa = 10.27 GIINKK301 pKa = 8.77 FF302 pKa = 3.25
Molecular weight: 34.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.728
IPC2_protein 8.858
IPC_protein 8.77
Toseland 9.297
ProMoST 9.151
Dawson 9.619
Bjellqvist 9.326
Wikipedia 9.838
Rodwell 9.823
Grimsley 9.736
Solomon 9.663
Lehninger 9.619
Nozaki 9.268
DTASelect 9.326
Thurlkill 9.428
EMBOSS 9.736
Sillero 9.531
Patrickios 4.533
IPC_peptide 9.648
IPC2_peptide 7.834
IPC2.peptide.svr19 7.776
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1295
71
523
259.0
29.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.413 ± 1.154
1.236 ± 0.619
5.251 ± 1.029
6.641 ± 0.563
4.788 ± 0.732
6.409 ± 0.501
2.394 ± 0.755
4.865 ± 0.429
5.714 ± 1.016
9.035 ± 1.076
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.78 ± 0.289
6.178 ± 1.459
4.788 ± 0.641
5.946 ± 1.489
5.405 ± 0.743
6.1 ± 0.953
4.942 ± 0.44
3.784 ± 0.896
1.544 ± 0.313
4.788 ± 0.812
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here