Prochlorococcus marinus (strain MIT 9301)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Prochloraceae; Prochlorococcus; Prochlorococcus marinus

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1906 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A3PDW5|A3PDW5_PROM0 Uncharacterized protein OS=Prochlorococcus marinus (strain MIT 9301) OX=167546 GN=P9301_13171 PE=4 SV=1
MM1 pKa = 7.45SALKK5 pKa = 10.61SFAALSIASMSILPLTPEE23 pKa = 4.78LKK25 pKa = 10.59ADD27 pKa = 3.99DD28 pKa = 4.26YY29 pKa = 11.96EE30 pKa = 5.27KK31 pKa = 11.26GFYY34 pKa = 8.86GTIGAGAGTFSDD46 pKa = 4.33LLLVGTPFALAFDD59 pKa = 4.59PGFSYY64 pKa = 10.6EE65 pKa = 4.13GSFGYY70 pKa = 10.62DD71 pKa = 3.03FGKK74 pKa = 10.04HH75 pKa = 4.31FRR77 pKa = 11.84ADD79 pKa = 3.08VSYY82 pKa = 10.3TNTISTIVTDD92 pKa = 3.91HH93 pKa = 5.37QAKK96 pKa = 9.78FGSIILNGYY105 pKa = 8.95IDD107 pKa = 5.95LPIEE111 pKa = 4.26DD112 pKa = 4.58TKK114 pKa = 9.44WTPFIGAGYY123 pKa = 10.63GSTNVDD129 pKa = 2.99AVNLCTAGGTDD140 pKa = 3.92DD141 pKa = 4.52CTDD144 pKa = 3.56DD145 pKa = 3.43VATYY149 pKa = 9.98SLSGGISYY157 pKa = 11.0ALNSDD162 pKa = 3.35TDD164 pKa = 3.45ITGKK168 pKa = 8.26ITYY171 pKa = 10.14LGFDD175 pKa = 4.71TINVTDD181 pKa = 5.18DD182 pKa = 3.47GTLVTVTEE190 pKa = 4.4SEE192 pKa = 4.39TLSAHH197 pKa = 6.27IGVKK201 pKa = 10.57FKK203 pKa = 11.03FF204 pKa = 3.66

Molecular weight: 21.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A3PE45|A3PE45_PROM0 Uncharacterized protein OS=Prochlorococcus marinus (strain MIT 9301) OX=167546 GN=P9301_13971 PE=4 SV=1
MM1 pKa = 7.41TKK3 pKa = 9.05RR4 pKa = 11.84TFGGTSRR11 pKa = 11.84KK12 pKa = 9.13RR13 pKa = 11.84KK14 pKa = 8.17RR15 pKa = 11.84VSGFRR20 pKa = 11.84VRR22 pKa = 11.84MRR24 pKa = 11.84SHH26 pKa = 6.25TGRR29 pKa = 11.84RR30 pKa = 11.84VIKK33 pKa = 10.15SRR35 pKa = 11.84RR36 pKa = 11.84QKK38 pKa = 9.67GRR40 pKa = 11.84EE41 pKa = 3.79RR42 pKa = 11.84IAVV45 pKa = 3.46

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1906

0

1906

496198

29

1523

260.3

29.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.401 ± 0.06

1.164 ± 0.022

5.128 ± 0.049

6.73 ± 0.057

4.955 ± 0.053

6.261 ± 0.055

1.498 ± 0.024

9.04 ± 0.065

8.605 ± 0.067

10.744 ± 0.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.857 ± 0.025

6.494 ± 0.067

3.631 ± 0.03

3.035 ± 0.027

3.921 ± 0.04

7.732 ± 0.043

4.438 ± 0.036

5.27 ± 0.051

1.256 ± 0.026

2.838 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski