Rhodococcus phage REQ1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 85 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G9FHB8|G9FHB8_9CAUD Uncharacterized protein OS=Rhodococcus phage REQ1 OX=1109712 PE=4 SV=1
MM1 pKa = 7.51SPQRR5 pKa = 11.84LWPYY9 pKa = 9.64TLVAVAAAGTLAFAGCAALSVGDD32 pKa = 4.08GGKK35 pKa = 8.24STVDD39 pKa = 3.4TPPFDD44 pKa = 4.14PFSTSQHH51 pKa = 5.49TADD54 pKa = 4.72ADD56 pKa = 4.07TPPMMFDD63 pKa = 3.66GDD65 pKa = 3.95TGTLRR70 pKa = 11.84VPADD74 pKa = 3.71IAPGTYY80 pKa = 8.55TATPTGEE87 pKa = 3.99GFYY90 pKa = 11.03SVLTCWADD98 pKa = 3.27HH99 pKa = 6.02PSCYY103 pKa = 10.65GEE105 pKa = 3.72TAYY108 pKa = 8.78ITEE111 pKa = 4.28PTKK114 pKa = 10.25ITVPPDD120 pKa = 3.3AVGLTIAYY128 pKa = 9.31IDD130 pKa = 3.79LRR132 pKa = 11.84GG133 pKa = 3.23

Molecular weight: 13.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G9FHB0|G9FHB0_9CAUD Uncharacterized protein OS=Rhodococcus phage REQ1 OX=1109712 PE=4 SV=1
MM1 pKa = 7.64GSHH4 pKa = 6.56NAYY7 pKa = 8.45TPRR10 pKa = 11.84RR11 pKa = 11.84EE12 pKa = 4.48PIPSPWLVLGRR23 pKa = 11.84LVGWGLVMMVGLFLFMFVFAIITTALL49 pKa = 3.36

Molecular weight: 5.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

85

0

85

15833

39

1447

186.3

20.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.339 ± 0.421

0.973 ± 0.161

6.423 ± 0.298

6.038 ± 0.416

3.038 ± 0.172

8.868 ± 0.45

2.154 ± 0.215

4.421 ± 0.276

3.556 ± 0.233

7.958 ± 0.34

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.983 ± 0.204

3.347 ± 0.272

5.04 ± 0.244

3.581 ± 0.178

7.004 ± 0.522

5.987 ± 0.444

7.188 ± 0.61

7.484 ± 0.24

2.128 ± 0.273

2.488 ± 0.247
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski