Leptolinea tardivitalis
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2936 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0P6WZT5|A0A0P6WZT5_9CHLR Uncharacterized protein OS=Leptolinea tardivitalis OX=229920 GN=ADM99_02290 PE=4 SV=1
MM1 pKa = 7.52 KK2 pKa = 10.38 KK3 pKa = 9.83 ADD5 pKa = 3.56 FVIRR9 pKa = 11.84 VLVLFSMIVSLLAGPGFIPASVVSAKK35 pKa = 10.36 PSAQEE40 pKa = 4.38 EE41 pKa = 4.86 IPTTAPGNVPTDD53 pKa = 3.69 EE54 pKa = 4.26 PTATPTEE61 pKa = 4.29 IPTEE65 pKa = 4.29 VPTSVPTEE73 pKa = 4.12 VPTEE77 pKa = 3.99 EE78 pKa = 4.26 PVIIPTDD85 pKa = 3.5 IPTEE89 pKa = 4.2 IPSPTPTVDD98 pKa = 2.96 PSLIVTEE105 pKa = 4.91 IPTATPTEE113 pKa = 4.52 TVSVTPTITEE123 pKa = 4.38 TPTEE127 pKa = 4.25 TPEE130 pKa = 5.0 SEE132 pKa = 4.19 GQPFSHH138 pKa = 7.5 LEE140 pKa = 3.65 FAALDD145 pKa = 4.04 DD146 pKa = 4.07 GTPVEE151 pKa = 4.28 EE152 pKa = 4.27 LVIIGSPNPPEE163 pKa = 5.32 GIDD166 pKa = 3.49 RR167 pKa = 11.84 PISAIAMTEE176 pKa = 4.06 GEE178 pKa = 4.67 TEE180 pKa = 4.19 GLTNPLAVPAYY191 pKa = 8.66 NWVYY195 pKa = 10.51 GCSSVSAAMVAGFYY209 pKa = 10.91 DD210 pKa = 3.34 RR211 pKa = 11.84 GGYY214 pKa = 8.66 PNIYY218 pKa = 9.34 TGPTDD223 pKa = 3.75 GGVMPMNNSVWPTWTDD239 pKa = 2.97 SSGDD243 pKa = 3.69 TYY245 pKa = 11.62 PNLPLAASKK254 pKa = 10.66 QGGWTYY260 pKa = 10.71 YY261 pKa = 10.06 PWFHH265 pKa = 6.95
Molecular weight: 28.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.744
IPC2_protein 3.846
IPC_protein 3.783
Toseland 3.605
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.617
Rodwell 3.617
Grimsley 3.516
Solomon 3.719
Lehninger 3.681
Nozaki 3.859
DTASelect 3.986
Thurlkill 3.63
EMBOSS 3.63
Sillero 3.897
Patrickios 0.985
IPC_peptide 3.732
IPC2_peptide 3.872
IPC2.peptide.svr19 3.792
Protein with the highest isoelectric point:
>tr|A0A0P6X7A0|A0A0P6X7A0_9CHLR Uncharacterized protein OS=Leptolinea tardivitalis OX=229920 GN=ADM99_11690 PE=4 SV=1
MM1 pKa = 7.62 KK2 pKa = 10.19 KK3 pKa = 9.77 QAGQAKK9 pKa = 10.17 SGLLDD14 pKa = 5.38 LISTSPLIGRR24 pKa = 11.84 VDD26 pKa = 3.35 ANGIHH31 pKa = 6.37 LTYY34 pKa = 9.47 QTSTRR39 pKa = 11.84 LRR41 pKa = 11.84 LHH43 pKa = 6.84 LPLPHH48 pKa = 7.04 PNVSPLEE55 pKa = 4.26 AGGARR60 pKa = 11.84 LIDD63 pKa = 3.93 HH64 pKa = 7.03 RR65 pKa = 11.84 HH66 pKa = 4.57 NPYY69 pKa = 10.48 RR70 pKa = 11.84 LLPPCIGLRR79 pKa = 3.55
Molecular weight: 8.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.341
IPC2_protein 9.736
IPC_protein 10.599
Toseland 10.672
ProMoST 10.906
Dawson 10.774
Bjellqvist 10.511
Wikipedia 10.994
Rodwell 10.965
Grimsley 10.833
Solomon 10.906
Lehninger 10.877
Nozaki 10.657
DTASelect 10.496
Thurlkill 10.672
EMBOSS 11.067
Sillero 10.701
Patrickios 10.774
IPC_peptide 10.906
IPC2_peptide 9.589
IPC2.peptide.svr19 8.683
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2936
0
2936
975580
48
3940
332.3
36.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.252 ± 0.043
0.955 ± 0.015
5.214 ± 0.031
5.881 ± 0.052
4.273 ± 0.036
7.32 ± 0.043
1.871 ± 0.021
7.199 ± 0.044
4.764 ± 0.039
10.4 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.569 ± 0.02
3.994 ± 0.03
4.932 ± 0.031
3.676 ± 0.036
5.102 ± 0.04
6.438 ± 0.039
5.757 ± 0.053
6.946 ± 0.035
1.456 ± 0.024
3.001 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here