Pseudoxanthomonas sp. Root65

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Pseudoxanthomonas; unclassified Pseudoxanthomonas

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3422 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q8FH27|A0A0Q8FH27_9GAMM Phosphoglycerate kinase OS=Pseudoxanthomonas sp. Root65 OX=1736576 GN=pgk PE=3 SV=1
MM1 pKa = 6.27TTVVSLPTAAAAPDD15 pKa = 3.85YY16 pKa = 10.98QSIDD20 pKa = 3.26RR21 pKa = 11.84PLNFTPAAAAKK32 pKa = 9.72VRR34 pKa = 11.84EE35 pKa = 4.53LIQEE39 pKa = 4.23EE40 pKa = 4.79GSDD43 pKa = 3.64ALALRR48 pKa = 11.84VYY50 pKa = 9.98IQGGGCSGFQYY61 pKa = 10.65GFEE64 pKa = 4.07FDD66 pKa = 3.9EE67 pKa = 4.48NRR69 pKa = 11.84ADD71 pKa = 4.77DD72 pKa = 4.89DD73 pKa = 4.28VAVDD77 pKa = 3.63TDD79 pKa = 4.39GVSLLVDD86 pKa = 4.08PLSLQYY92 pKa = 11.71LMGAEE97 pKa = 3.92VDD99 pKa = 3.92YY100 pKa = 11.68SEE102 pKa = 4.94SLQGAQFVIRR112 pKa = 11.84NPNAKK117 pKa = 6.55TTCGCGSSFSMM128 pKa = 5.09

Molecular weight: 13.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q8FAS0|A0A0Q8FAS0_9GAMM Transcription-repair-coupling factor OS=Pseudoxanthomonas sp. Root65 OX=1736576 GN=mfd PE=3 SV=1
MM1 pKa = 7.51PALAAFALDD10 pKa = 3.38ALIFIGLAWGVWRR23 pKa = 11.84VLGRR27 pKa = 11.84AIPIAVIPIIIGLGLAATGVLPAGWGVPSKK57 pKa = 11.07LGGQLGFVGVLLLAFTAGLEE77 pKa = 4.4TRR79 pKa = 11.84QSAHH83 pKa = 6.75LPPTSEE89 pKa = 3.98AAPLPRR95 pKa = 11.84TQALRR100 pKa = 11.84FMASAGMALLLPFALGTLAAHH121 pKa = 6.75YY122 pKa = 9.74YY123 pKa = 8.52FTGLPGWDD131 pKa = 3.65APRR134 pKa = 11.84GGAWMGSMAIGLCLAVSALPVLIGIVRR161 pKa = 11.84EE162 pKa = 4.11LGPLHH167 pKa = 7.35RR168 pKa = 11.84PLTQLALRR176 pKa = 11.84IAVIDD181 pKa = 5.1DD182 pKa = 3.33AVLWIGLALLLLAAEE197 pKa = 5.06GGSLLAGASVLHH209 pKa = 5.67VLALGVAILLAAAGTLAQRR228 pKa = 11.84LRR230 pKa = 11.84APPPAWLVWVSLPVFMVAGAWASSQLGLHH259 pKa = 6.62ALLGAYY265 pKa = 9.88FGGAMVPPAWGRR277 pKa = 11.84RR278 pKa = 11.84LPVEE282 pKa = 3.97RR283 pKa = 11.84LGQFALIGLAPLFFGHH299 pKa = 6.53SGLKK303 pKa = 9.54IDD305 pKa = 4.83GSVLGWASLQASLALLVLAVVAKK328 pKa = 10.08LAAVLACPPASHH340 pKa = 7.55LSRR343 pKa = 11.84RR344 pKa = 11.84DD345 pKa = 3.34ALAVGTLLQCKK356 pKa = 9.89GLMEE360 pKa = 4.36IVAATILRR368 pKa = 11.84DD369 pKa = 3.3KK370 pKa = 11.32GLLSEE375 pKa = 4.57HH376 pKa = 6.72AFAALVTLAVLSTLLTGPFFRR397 pKa = 11.84ALVKK401 pKa = 10.45RR402 pKa = 11.84RR403 pKa = 11.84AAQPQSGSQPAA414 pKa = 3.15

Molecular weight: 42.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3422

0

3422

1126097

41

3138

329.1

35.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.061 ± 0.062

0.777 ± 0.012

6.106 ± 0.034

5.24 ± 0.037

3.382 ± 0.028

8.547 ± 0.045

2.258 ± 0.023

4.059 ± 0.03

2.746 ± 0.037

10.714 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.193 ± 0.02

2.4 ± 0.029

5.355 ± 0.034

3.729 ± 0.023

7.627 ± 0.047

4.989 ± 0.034

5.247 ± 0.044

7.682 ± 0.034

1.551 ± 0.02

2.334 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski