Rhesus cytomegalovirus (strain 68-1) (RhCMV)
Average proteome isoelectric point is 7.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 223 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q7TFW5|Q7TFW5_RHCM6 Rh16 OS=Rhesus cytomegalovirus (strain 68-1) OX=103930 PE=4 SV=1
MM1 pKa = 7.54 FISGVLVQEE10 pKa = 4.42 VVVVVYY16 pKa = 10.14 EE17 pKa = 4.22 LFLDD21 pKa = 3.92 TFVDD25 pKa = 3.48 SFDD28 pKa = 3.55 NVFVYY33 pKa = 10.0 DD34 pKa = 3.51 VRR36 pKa = 11.84 VTSEE40 pKa = 4.03 VQDD43 pKa = 3.88 AFCDD47 pKa = 3.91 VYY49 pKa = 11.63 EE50 pKa = 4.16 MLVTVLDD57 pKa = 3.37 IGVVVFPCEE66 pKa = 4.07 VLADD70 pKa = 4.18 PYY72 pKa = 11.21 VVVHH76 pKa = 6.07 VVLLIYY82 pKa = 10.38 EE83 pKa = 4.44 LFVDD87 pKa = 4.12 LGVVEE92 pKa = 4.84 SVVNSVVILTLDD104 pKa = 3.39 EE105 pKa = 4.87 LFVEE109 pKa = 4.87 LSVARR114 pKa = 11.84 IGQNMTKK121 pKa = 10.61
Molecular weight: 13.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.877
IPC2_protein 3.592
IPC_protein 3.528
Toseland 3.338
ProMoST 3.719
Dawson 3.528
Bjellqvist 3.694
Wikipedia 3.478
Rodwell 3.376
Grimsley 3.249
Solomon 3.503
Lehninger 3.452
Nozaki 3.668
DTASelect 3.846
Thurlkill 3.401
EMBOSS 3.49
Sillero 3.656
Patrickios 0.223
IPC_peptide 3.49
IPC2_peptide 3.63
IPC2.peptide.svr19 3.665
Protein with the highest isoelectric point:
>tr|Q7TFS9|Q7TFS9_RHCM6 Rh52 OS=Rhesus cytomegalovirus (strain 68-1) OX=103930 PE=4 SV=1
MM1 pKa = 8.27 KK2 pKa = 10.46 MMMTTMKK9 pKa = 9.62 RR10 pKa = 11.84 QRR12 pKa = 11.84 WMVMRR17 pKa = 11.84 AWATPPRR24 pKa = 11.84 PMIGLRR30 pKa = 11.84 GRR32 pKa = 11.84 HH33 pKa = 4.89 LLGRR37 pKa = 11.84 TSCYY41 pKa = 9.87 LYY43 pKa = 10.42 KK44 pKa = 10.33 IKK46 pKa = 10.56 FQITGSFPTCKK57 pKa = 9.65 RR58 pKa = 11.84 RR59 pKa = 11.84 SKK61 pKa = 10.79 AVSTAARR68 pKa = 11.84 SVQWVYY74 pKa = 9.47 FWEE77 pKa = 4.35 MRR79 pKa = 11.84 VRR81 pKa = 11.84 WCLKK85 pKa = 9.41 PQLDD89 pKa = 3.66 MDD91 pKa = 4.93 GIEE94 pKa = 4.65 AFCEE98 pKa = 4.82 FYY100 pKa = 10.16 STLSIRR106 pKa = 11.84 DD107 pKa = 3.11 QDD109 pKa = 3.77 DD110 pKa = 3.59 FNRR113 pKa = 11.84 EE114 pKa = 3.31 VDD116 pKa = 3.51 LGVQHH121 pKa = 7.28 RR122 pKa = 11.84 RR123 pKa = 11.84 LQQLRR128 pKa = 11.84 RR129 pKa = 11.84 ARR131 pKa = 11.84 QNLKK135 pKa = 10.39 RR136 pKa = 11.84 EE137 pKa = 4.62 CICSGGG143 pKa = 3.42
Molecular weight: 17.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.663
IPC_protein 10.54
Toseland 10.73
ProMoST 10.482
Dawson 10.818
Bjellqvist 10.57
Wikipedia 11.052
Rodwell 10.979
Grimsley 10.862
Solomon 10.965
Lehninger 10.935
Nozaki 10.745
DTASelect 10.555
Thurlkill 10.73
EMBOSS 11.14
Sillero 10.76
Patrickios 10.701
IPC_peptide 10.979
IPC2_peptide 9.867
IPC2.peptide.svr19 8.555
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
223
0
223
66627
100
2177
298.8
33.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.64 ± 0.179
2.675 ± 0.095
4.479 ± 0.137
4.824 ± 0.133
4.171 ± 0.124
4.84 ± 0.12
3.284 ± 0.104
5.094 ± 0.13
3.584 ± 0.136
9.886 ± 0.193
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.534 ± 0.077
4.127 ± 0.119
5.654 ± 0.194
3.904 ± 0.137
7.009 ± 0.202
7.967 ± 0.17
7.213 ± 0.26
7.114 ± 0.158
1.483 ± 0.092
3.518 ± 0.097
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here