Mesoflavibacter sabulilitoris

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Mesoflavibacter

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2809 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T1NIG9|A0A2T1NIG9_9FLAO Cytochrome c oxidase subunit I OS=Mesoflavibacter sabulilitoris OX=1520893 GN=C7H61_04465 PE=3 SV=1
MM1 pKa = 7.29KK2 pKa = 10.26QQLLLTVFILSFFSGLAQNSWKK24 pKa = 10.3KK25 pKa = 10.22ISSNSIEE32 pKa = 4.15NVNLKK37 pKa = 10.54KK38 pKa = 10.47FDD40 pKa = 4.49SNHH43 pKa = 5.87SALFSLDD50 pKa = 2.91IEE52 pKa = 4.27AFKK55 pKa = 11.16AKK57 pKa = 9.96LQSAPLRR64 pKa = 11.84SSSNGQSSTIVSIPDD79 pKa = 3.19EE80 pKa = 4.26TGKK83 pKa = 10.1ILQFRR88 pKa = 11.84VVEE91 pKa = 4.24APVLSKK97 pKa = 10.35EE98 pKa = 5.4LSIQYY103 pKa = 10.14PNIKK107 pKa = 9.18TYY109 pKa = 10.78LGSLVNDD116 pKa = 3.04VTTRR120 pKa = 11.84VRR122 pKa = 11.84FSVTPLGVNVMITRR136 pKa = 11.84ANQEE140 pKa = 3.84LTLIQPVSRR149 pKa = 11.84FSNVEE154 pKa = 3.69HH155 pKa = 6.8IAYY158 pKa = 9.09KK159 pKa = 10.24RR160 pKa = 11.84KK161 pKa = 10.36SINEE165 pKa = 3.69DD166 pKa = 2.99VKK168 pKa = 11.0RR169 pKa = 11.84FEE171 pKa = 4.74CLTDD175 pKa = 3.12EE176 pKa = 4.96TKK178 pKa = 10.41IRR180 pKa = 11.84DD181 pKa = 3.67QNIGQSRR188 pKa = 11.84MMDD191 pKa = 3.33ANDD194 pKa = 3.24QLLRR198 pKa = 11.84DD199 pKa = 3.69YY200 pKa = 10.8RR201 pKa = 11.84IAVSTNGEE209 pKa = 4.13YY210 pKa = 9.48TQFWDD215 pKa = 5.16DD216 pKa = 3.35GNAANGDD223 pKa = 3.85AQQDD227 pKa = 3.27ALAQVVSTLNRR238 pKa = 11.84VNEE241 pKa = 4.21VYY243 pKa = 10.27EE244 pKa = 3.96VDD246 pKa = 3.36MAITFTLVTGTEE258 pKa = 4.29IIFPDD263 pKa = 4.64PATDD267 pKa = 4.21PYY269 pKa = 10.35TGSFNSQLQNEE280 pKa = 4.56LTSTVGEE287 pKa = 3.92ANYY290 pKa = 10.66DD291 pKa = 3.44IGHH294 pKa = 7.04LFAYY298 pKa = 9.97GGNNGNAGCIGCVCEE313 pKa = 5.24DD314 pKa = 3.54NQKK317 pKa = 10.64GSGFSSHH324 pKa = 6.62SFLDD328 pKa = 3.87NDD330 pKa = 3.71GGAYY334 pKa = 9.62MSDD337 pKa = 3.93FFDD340 pKa = 3.54IDD342 pKa = 4.05YY343 pKa = 10.48VPHH346 pKa = 7.08EE347 pKa = 5.17IGHH350 pKa = 5.26QMGANHH356 pKa = 6.59TFAFNTEE363 pKa = 4.08GTGVNSEE370 pKa = 4.47PGSGTTIMGYY380 pKa = 10.88AGITGANDD388 pKa = 3.35VQDD391 pKa = 4.18HH392 pKa = 6.27SDD394 pKa = 3.29AYY396 pKa = 10.31FHH398 pKa = 5.96YY399 pKa = 10.64HH400 pKa = 7.14SINQILTNVASAPNNCATTTVITNNPPVANAGLDD434 pKa = 3.54YY435 pKa = 10.24TIPNGTAFVLKK446 pKa = 10.47GAANDD451 pKa = 3.93ADD453 pKa = 3.98SGDD456 pKa = 3.56VLTYY460 pKa = 9.03TWEE463 pKa = 4.31QIDD466 pKa = 3.93SGSTTSTSFGPAHH479 pKa = 6.79TGPVWRR485 pKa = 11.84SRR487 pKa = 11.84PPSTNPDD494 pKa = 2.74RR495 pKa = 11.84YY496 pKa = 8.66MPIIEE501 pKa = 4.05RR502 pKa = 11.84VIAGEE507 pKa = 3.93LTEE510 pKa = 4.29TNPVEE515 pKa = 4.26TFDD518 pKa = 3.56NSSWEE523 pKa = 4.28TVSTIGRR530 pKa = 11.84TLNFALTVRR539 pKa = 11.84DD540 pKa = 3.54RR541 pKa = 11.84SDD543 pKa = 3.1AGGVGQTPQSDD554 pKa = 4.06FDD556 pKa = 4.15TMTVTVDD563 pKa = 3.61GSSGPFAVTSQTSAVTWDD581 pKa = 3.26AGSTEE586 pKa = 4.41TITWDD591 pKa = 3.38VAGTNTGAVNTPTVNIKK608 pKa = 10.73LSTDD612 pKa = 2.95GGFTYY617 pKa = 9.62PYY619 pKa = 10.79VLATDD624 pKa = 3.84VPNDD628 pKa = 3.64GSHH631 pKa = 7.76DD632 pKa = 3.21ITVPVTGGDD641 pKa = 3.31TATARR646 pKa = 11.84VMVEE650 pKa = 3.58GNNNIFYY657 pKa = 10.49AINSTNFNIQEE668 pKa = 4.17SEE670 pKa = 4.28FVITANNTPVDD681 pKa = 3.75VCSPNDD687 pKa = 3.34AVFNFTYY694 pKa = 9.58NTFLGFSGTTTFSTSGLPAGTTATITPTTATADD727 pKa = 3.66GTAVTVTVTGIGGLSIGNYY746 pKa = 9.37PFTLTGTSGSITSSANVEE764 pKa = 4.34FNVNDD769 pKa = 4.07TNFSTINTLTPALGVTDD786 pKa = 4.03VAPSNAVFTWDD797 pKa = 4.28LDD799 pKa = 4.15PNATSYY805 pKa = 11.15EE806 pKa = 3.96LDD808 pKa = 3.16IATDD812 pKa = 3.41SGFTSIIASQTLTANNYY829 pKa = 7.86TSTSLATQTMYY840 pKa = 8.47WWRR843 pKa = 11.84VRR845 pKa = 11.84AVNDD849 pKa = 4.13CGSGAYY855 pKa = 7.73ATSNFEE861 pKa = 4.25TANIACNTYY870 pKa = 9.53TSNDD874 pKa = 3.48TPIAIPDD881 pKa = 3.86SNTTGISSIITVTDD895 pKa = 3.21VSSIIDD901 pKa = 3.48VNVTINITHH910 pKa = 6.64SYY912 pKa = 10.72VSDD915 pKa = 3.5LTVSLVSPAGTEE927 pKa = 3.37IDD929 pKa = 3.84LSFEE933 pKa = 4.24NGGSGMNYY941 pKa = 8.81TDD943 pKa = 3.87TVFDD947 pKa = 5.28DD948 pKa = 4.96DD949 pKa = 4.4AANPIASGTAPFTGVYY965 pKa = 9.79QPTEE969 pKa = 3.78ALSILNSEE977 pKa = 4.79FSSGDD982 pKa = 2.88WTLKK986 pKa = 10.91VIDD989 pKa = 4.62SYY991 pKa = 12.18GFDD994 pKa = 3.49TGDD997 pKa = 3.32LVNWSIEE1004 pKa = 4.07ICGSPQNDD1012 pKa = 3.45TDD1014 pKa = 4.37GDD1016 pKa = 4.48GIPDD1020 pKa = 3.92GSDD1023 pKa = 2.84NCVNTPNFDD1032 pKa = 3.54QADD1035 pKa = 3.5VDD1037 pKa = 3.91GDD1039 pKa = 4.47GIGDD1043 pKa = 3.75VCDD1046 pKa = 5.41DD1047 pKa = 5.68DD1048 pKa = 5.06IDD1050 pKa = 4.42NDD1052 pKa = 4.98GILNTSDD1059 pKa = 2.88NCIYY1063 pKa = 10.31TPNADD1068 pKa = 3.55QADD1071 pKa = 3.78ADD1073 pKa = 4.1GDD1075 pKa = 4.53GIGDD1079 pKa = 3.73VCDD1082 pKa = 3.99VEE1084 pKa = 4.6CTVYY1088 pKa = 10.76SATDD1092 pKa = 3.64LPITIASTGADD1103 pKa = 3.13ANYY1106 pKa = 9.82TSVINILEE1114 pKa = 4.48DD1115 pKa = 3.79APIADD1120 pKa = 3.96VNVTISINHH1129 pKa = 5.72TWNSDD1134 pKa = 3.16LDD1136 pKa = 3.52ISLISPAGTIIEE1148 pKa = 4.45LTTGNGGSSDD1158 pKa = 3.72NYY1160 pKa = 9.17TNTVFDD1166 pKa = 3.76QDD1168 pKa = 3.33ATASITTGSAPFTGVFMPEE1187 pKa = 3.82GDD1189 pKa = 3.81LSLLNGEE1196 pKa = 4.32MSAGNWTLSVDD1207 pKa = 3.7DD1208 pKa = 4.54TYY1210 pKa = 11.85GPEE1213 pKa = 4.32DD1214 pKa = 3.88GGTILSFDD1222 pKa = 4.53LEE1224 pKa = 4.45LCLFSALSIGNDD1236 pKa = 3.1VLNNTEE1242 pKa = 3.94FSVYY1246 pKa = 10.05PNPNNGEE1253 pKa = 3.91FTIKK1257 pKa = 9.7MKK1259 pKa = 9.82NTVFKK1264 pKa = 10.2EE1265 pKa = 3.84DD1266 pKa = 3.47VEE1268 pKa = 4.53IYY1270 pKa = 10.67VYY1272 pKa = 10.22DD1273 pKa = 3.22IRR1275 pKa = 11.84GRR1277 pKa = 11.84KK1278 pKa = 8.76VFYY1281 pKa = 10.72QSFKK1285 pKa = 10.47NSNNFIQQIRR1295 pKa = 11.84LNDD1298 pKa = 3.76VEE1300 pKa = 4.52SGMYY1304 pKa = 9.49ILNVTDD1310 pKa = 4.5GEE1312 pKa = 4.36NTKK1315 pKa = 9.01TKK1317 pKa = 10.77KK1318 pKa = 10.78LIIDD1322 pKa = 3.93

Molecular weight: 141.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T1NKN2|A0A2T1NKN2_9FLAO MAM protein (Fragment) OS=Mesoflavibacter sabulilitoris OX=1520893 GN=C7H61_02525 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.27RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.09GRR40 pKa = 11.84KK41 pKa = 7.97KK42 pKa = 10.62LSVSSEE48 pKa = 4.03TRR50 pKa = 11.84HH51 pKa = 5.96KK52 pKa = 10.68KK53 pKa = 9.8

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2809

0

2809

940475

38

2410

334.8

37.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.127 ± 0.044

0.744 ± 0.018

5.888 ± 0.04

6.447 ± 0.044

5.296 ± 0.037

5.97 ± 0.05

1.686 ± 0.022

8.05 ± 0.045

8.12 ± 0.083

9.217 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.991 ± 0.023

6.842 ± 0.053

3.238 ± 0.03

3.589 ± 0.03

3.04 ± 0.029

6.289 ± 0.037

6.202 ± 0.058

6.174 ± 0.033

0.95 ± 0.016

4.14 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski