Amino acid dipepetide frequency for Mycobacterium phage Philonius

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.217AlaAla: 19.217 ± 2.808
0.495AlaCys: 0.495 ± 0.221
9.397AlaAsp: 9.397 ± 0.85
8.902AlaGlu: 8.902 ± 1.198
3.109AlaPhe: 3.109 ± 0.488
9.891AlaGly: 9.891 ± 1.311
2.473AlaHis: 2.473 ± 0.406
5.511AlaIle: 5.511 ± 0.667
3.674AlaLys: 3.674 ± 0.683
9.043AlaLeu: 9.043 ± 0.906
2.261AlaMet: 2.261 ± 0.426
3.391AlaAsn: 3.391 ± 0.644
7.136AlaPro: 7.136 ± 0.756
5.299AlaGln: 5.299 ± 0.763
7.701AlaArg: 7.701 ± 0.818
6.217AlaSer: 6.217 ± 0.658
8.408AlaThr: 8.408 ± 0.844
7.348AlaVal: 7.348 ± 0.72
1.837AlaTrp: 1.837 ± 0.434
1.978AlaTyr: 1.978 ± 0.396
0.0AlaXaa: 0.0 ± 0.0
Cys
1.554CysAla: 1.554 ± 0.329
0.212CysCys: 0.212 ± 0.109
1.06CysAsp: 1.06 ± 0.292
0.495CysGlu: 0.495 ± 0.211
0.071CysPhe: 0.071 ± 0.072
1.484CysGly: 1.484 ± 0.425
0.212CysHis: 0.212 ± 0.127
0.141CysIle: 0.141 ± 0.103
0.141CysLys: 0.141 ± 0.095
0.707CysLeu: 0.707 ± 0.219
0.212CysMet: 0.212 ± 0.122
0.353CysAsn: 0.353 ± 0.144
1.554CysPro: 1.554 ± 0.428
0.707CysGln: 0.707 ± 0.251
0.918CysArg: 0.918 ± 0.271
0.495CysSer: 0.495 ± 0.18
0.636CysThr: 0.636 ± 0.201
0.707CysVal: 0.707 ± 0.208
0.212CysTrp: 0.212 ± 0.133
0.141CysTyr: 0.141 ± 0.092
0.0CysXaa: 0.0 ± 0.0
Asp
8.478AspAla: 8.478 ± 0.827
1.201AspCys: 1.201 ± 0.364
5.228AspAsp: 5.228 ± 0.739
4.592AspGlu: 4.592 ± 0.703
1.554AspPhe: 1.554 ± 0.341
6.783AspGly: 6.783 ± 0.654
1.342AspHis: 1.342 ± 0.327
3.109AspIle: 3.109 ± 0.427
1.484AspLys: 1.484 ± 0.327
6.147AspLeu: 6.147 ± 0.641
1.554AspMet: 1.554 ± 0.282
1.696AspAsn: 1.696 ± 0.394
4.027AspPro: 4.027 ± 0.583
2.402AspGln: 2.402 ± 0.439
4.027AspArg: 4.027 ± 0.723
3.745AspSer: 3.745 ± 0.569
3.038AspThr: 3.038 ± 0.492
3.533AspVal: 3.533 ± 0.489
1.06AspTrp: 1.06 ± 0.303
1.272AspTyr: 1.272 ± 0.325
0.0AspXaa: 0.0 ± 0.0
Glu
5.652GluAla: 5.652 ± 0.643
0.848GluCys: 0.848 ± 0.253
3.674GluAsp: 3.674 ± 0.497
1.272GluGlu: 1.272 ± 0.322
2.614GluPhe: 2.614 ± 0.461
2.897GluGly: 2.897 ± 0.565
1.413GluHis: 1.413 ± 0.31
2.897GluIle: 2.897 ± 0.498
1.837GluLys: 1.837 ± 0.322
5.37GluLeu: 5.37 ± 0.735
1.837GluMet: 1.837 ± 0.42
1.696GluAsn: 1.696 ± 0.311
3.179GluPro: 3.179 ± 0.603
2.967GluGln: 2.967 ± 0.446
4.239GluArg: 4.239 ± 0.733
3.109GluSer: 3.109 ± 0.487
4.027GluThr: 4.027 ± 0.66
3.815GluVal: 3.815 ± 0.569
0.989GluTrp: 0.989 ± 0.266
1.342GluTyr: 1.342 ± 0.384
0.0GluXaa: 0.0 ± 0.0
Phe
3.391PheAla: 3.391 ± 0.468
0.353PheCys: 0.353 ± 0.19
1.272PheAsp: 1.272 ± 0.38
1.625PheGlu: 1.625 ± 0.355
0.989PhePhe: 0.989 ± 0.321
3.462PheGly: 3.462 ± 0.474
0.848PheHis: 0.848 ± 0.262
1.413PheIle: 1.413 ± 0.31
0.848PheLys: 0.848 ± 0.199
1.554PheLeu: 1.554 ± 0.327
0.636PheMet: 0.636 ± 0.265
0.848PheAsn: 0.848 ± 0.233
1.06PhePro: 1.06 ± 0.265
0.565PheGln: 0.565 ± 0.207
1.484PheArg: 1.484 ± 0.314
1.554PheSer: 1.554 ± 0.31
2.402PheThr: 2.402 ± 0.364
2.543PheVal: 2.543 ± 0.432
0.212PheTrp: 0.212 ± 0.126
1.13PheTyr: 1.13 ± 0.297
0.0PheXaa: 0.0 ± 0.0
Gly
8.831GlyAla: 8.831 ± 1.304
0.848GlyCys: 0.848 ± 0.358
5.652GlyAsp: 5.652 ± 0.629
3.745GlyGlu: 3.745 ± 0.674
2.685GlyPhe: 2.685 ± 0.516
9.114GlyGly: 9.114 ± 1.51
1.554GlyHis: 1.554 ± 0.304
4.098GlyIle: 4.098 ± 0.789
2.331GlyLys: 2.331 ± 0.366
6.5GlyLeu: 6.5 ± 0.668
1.554GlyMet: 1.554 ± 0.283
3.25GlyAsn: 3.25 ± 0.616
4.804GlyPro: 4.804 ± 0.638
4.31GlyGln: 4.31 ± 0.641
5.511GlyArg: 5.511 ± 0.511
4.875GlySer: 4.875 ± 0.672
6.429GlyThr: 6.429 ± 0.884
6.924GlyVal: 6.924 ± 0.683
2.402GlyTrp: 2.402 ± 0.386
2.685GlyTyr: 2.685 ± 0.59
0.0GlyXaa: 0.0 ± 0.0
His
1.625HisAla: 1.625 ± 0.393
0.565HisCys: 0.565 ± 0.196
1.554HisAsp: 1.554 ± 0.31
1.342HisGlu: 1.342 ± 0.336
0.353HisPhe: 0.353 ± 0.144
1.766HisGly: 1.766 ± 0.364
0.918HisHis: 0.918 ± 0.299
0.918HisIle: 0.918 ± 0.239
0.495HisLys: 0.495 ± 0.204
1.484HisLeu: 1.484 ± 0.423
0.353HisMet: 0.353 ± 0.164
0.424HisAsn: 0.424 ± 0.149
1.342HisPro: 1.342 ± 0.334
0.989HisGln: 0.989 ± 0.31
1.908HisArg: 1.908 ± 0.411
0.424HisSer: 0.424 ± 0.169
1.554HisThr: 1.554 ± 0.289
1.13HisVal: 1.13 ± 0.3
0.424HisTrp: 0.424 ± 0.142
0.848HisTyr: 0.848 ± 0.239
0.0HisXaa: 0.0 ± 0.0
Ile
5.864IleAla: 5.864 ± 0.589
0.141IleCys: 0.141 ± 0.086
3.391IleAsp: 3.391 ± 0.5
4.239IleGlu: 4.239 ± 0.428
0.777IlePhe: 0.777 ± 0.268
4.239IleGly: 4.239 ± 0.616
1.413IleHis: 1.413 ± 0.358
1.201IleIle: 1.201 ± 0.269
0.918IleLys: 0.918 ± 0.291
2.331IleLeu: 2.331 ± 0.429
0.212IleMet: 0.212 ± 0.135
2.12IleAsn: 2.12 ± 0.364
2.755IlePro: 2.755 ± 0.481
1.342IleGln: 1.342 ± 0.319
3.886IleArg: 3.886 ± 0.457
2.12IleSer: 2.12 ± 0.38
3.886IleThr: 3.886 ± 0.566
2.897IleVal: 2.897 ± 0.577
0.777IleTrp: 0.777 ± 0.247
0.848IleTyr: 0.848 ± 0.227
0.0IleXaa: 0.0 ± 0.0
Lys
3.886LysAla: 3.886 ± 0.868
0.353LysCys: 0.353 ± 0.177
0.918LysAsp: 0.918 ± 0.273
1.201LysGlu: 1.201 ± 0.225
0.918LysPhe: 0.918 ± 0.232
2.402LysGly: 2.402 ± 0.462
0.565LysHis: 0.565 ± 0.194
1.342LysIle: 1.342 ± 0.271
0.565LysLys: 0.565 ± 0.175
2.402LysLeu: 2.402 ± 0.492
0.636LysMet: 0.636 ± 0.218
0.565LysAsn: 0.565 ± 0.204
2.897LysPro: 2.897 ± 0.438
1.272LysGln: 1.272 ± 0.345
2.12LysArg: 2.12 ± 0.448
1.766LysSer: 1.766 ± 0.347
1.837LysThr: 1.837 ± 0.381
2.19LysVal: 2.19 ± 0.368
0.777LysTrp: 0.777 ± 0.219
0.777LysTyr: 0.777 ± 0.222
0.0LysXaa: 0.0 ± 0.0
Leu
10.456LeuAla: 10.456 ± 1.017
0.989LeuCys: 0.989 ± 0.277
5.228LeuAsp: 5.228 ± 0.628
3.038LeuGlu: 3.038 ± 0.343
2.543LeuPhe: 2.543 ± 0.46
7.772LeuGly: 7.772 ± 1.044
1.484LeuHis: 1.484 ± 0.343
3.462LeuIle: 3.462 ± 0.384
2.473LeuLys: 2.473 ± 0.518
6.994LeuLeu: 6.994 ± 0.724
0.848LeuMet: 0.848 ± 0.272
2.614LeuAsn: 2.614 ± 0.465
4.522LeuPro: 4.522 ± 0.767
2.614LeuGln: 2.614 ± 0.44
5.652LeuArg: 5.652 ± 0.724
3.956LeuSer: 3.956 ± 0.555
5.793LeuThr: 5.793 ± 0.764
5.158LeuVal: 5.158 ± 0.611
1.484LeuTrp: 1.484 ± 0.285
1.201LeuTyr: 1.201 ± 0.272
0.0LeuXaa: 0.0 ± 0.0
Met
3.038MetAla: 3.038 ± 0.473
0.212MetCys: 0.212 ± 0.151
0.495MetAsp: 0.495 ± 0.233
0.777MetGlu: 0.777 ± 0.285
0.989MetPhe: 0.989 ± 0.257
0.636MetGly: 0.636 ± 0.173
0.212MetHis: 0.212 ± 0.124
0.989MetIle: 0.989 ± 0.3
0.707MetLys: 0.707 ± 0.241
1.201MetLeu: 1.201 ± 0.287
0.495MetMet: 0.495 ± 0.155
0.848MetAsn: 0.848 ± 0.259
1.201MetPro: 1.201 ± 0.23
0.707MetGln: 0.707 ± 0.21
1.342MetArg: 1.342 ± 0.355
2.685MetSer: 2.685 ± 0.436
1.696MetThr: 1.696 ± 0.311
0.848MetVal: 0.848 ± 0.178
0.707MetTrp: 0.707 ± 0.289
0.353MetTyr: 0.353 ± 0.14
0.0MetXaa: 0.0 ± 0.0
Asn
3.25AsnAla: 3.25 ± 0.515
0.495AsnCys: 0.495 ± 0.175
1.484AsnAsp: 1.484 ± 0.288
1.201AsnGlu: 1.201 ± 0.32
0.918AsnPhe: 0.918 ± 0.258
3.533AsnGly: 3.533 ± 0.671
0.283AsnHis: 0.283 ± 0.171
1.625AsnIle: 1.625 ± 0.402
0.777AsnLys: 0.777 ± 0.29
3.109AsnLeu: 3.109 ± 0.528
0.424AsnMet: 0.424 ± 0.151
1.272AsnAsn: 1.272 ± 0.31
2.967AsnPro: 2.967 ± 0.421
1.342AsnGln: 1.342 ± 0.3
2.049AsnArg: 2.049 ± 0.329
1.484AsnSer: 1.484 ± 0.312
1.908AsnThr: 1.908 ± 0.321
1.766AsnVal: 1.766 ± 0.378
0.565AsnTrp: 0.565 ± 0.19
0.565AsnTyr: 0.565 ± 0.25
0.0AsnXaa: 0.0 ± 0.0
Pro
7.418ProAla: 7.418 ± 0.875
0.495ProCys: 0.495 ± 0.214
4.804ProAsp: 4.804 ± 0.569
4.804ProGlu: 4.804 ± 0.64
1.837ProPhe: 1.837 ± 0.362
6.217ProGly: 6.217 ± 0.894
1.272ProHis: 1.272 ± 0.263
2.473ProIle: 2.473 ± 0.408
1.413ProLys: 1.413 ± 0.333
4.31ProLeu: 4.31 ± 0.589
1.554ProMet: 1.554 ± 0.367
1.978ProAsn: 1.978 ± 0.369
4.168ProPro: 4.168 ± 0.564
2.19ProGln: 2.19 ± 0.353
2.897ProArg: 2.897 ± 0.414
3.25ProSer: 3.25 ± 0.541
4.31ProThr: 4.31 ± 0.519
4.804ProVal: 4.804 ± 0.545
1.484ProTrp: 1.484 ± 0.4
1.272ProTyr: 1.272 ± 0.387
0.0ProXaa: 0.0 ± 0.0
Gln
5.158GlnAla: 5.158 ± 0.931
0.353GlnCys: 0.353 ± 0.163
1.837GlnAsp: 1.837 ± 0.498
1.484GlnGlu: 1.484 ± 0.37
1.06GlnPhe: 1.06 ± 0.212
2.12GlnGly: 2.12 ± 0.377
1.201GlnHis: 1.201 ± 0.312
2.826GlnIle: 2.826 ± 0.496
1.13GlnLys: 1.13 ± 0.246
4.31GlnLeu: 4.31 ± 0.543
0.918GlnMet: 0.918 ± 0.219
0.636GlnAsn: 0.636 ± 0.245
2.402GlnPro: 2.402 ± 0.449
1.554GlnGln: 1.554 ± 0.443
3.815GlnArg: 3.815 ± 0.66
1.766GlnSer: 1.766 ± 0.401
2.473GlnThr: 2.473 ± 0.391
2.967GlnVal: 2.967 ± 0.473
0.636GlnTrp: 0.636 ± 0.174
0.848GlnTyr: 0.848 ± 0.248
0.0GlnXaa: 0.0 ± 0.0
Arg
7.065ArgAla: 7.065 ± 0.817
1.13ArgCys: 1.13 ± 0.329
4.946ArgAsp: 4.946 ± 0.744
4.239ArgGlu: 4.239 ± 0.681
1.837ArgPhe: 1.837 ± 0.479
5.087ArgGly: 5.087 ± 0.679
1.484ArgHis: 1.484 ± 0.364
3.674ArgIle: 3.674 ± 0.54
2.543ArgLys: 2.543 ± 0.459
4.592ArgLeu: 4.592 ± 0.458
2.19ArgMet: 2.19 ± 0.557
2.12ArgAsn: 2.12 ± 0.35
4.31ArgPro: 4.31 ± 0.554
2.897ArgGln: 2.897 ± 0.676
5.652ArgArg: 5.652 ± 0.83
2.473ArgSer: 2.473 ± 0.472
4.027ArgThr: 4.027 ± 0.593
4.38ArgVal: 4.38 ± 0.535
1.554ArgTrp: 1.554 ± 0.309
1.766ArgTyr: 1.766 ± 0.387
0.0ArgXaa: 0.0 ± 0.0
Ser
7.701SerAla: 7.701 ± 0.94
0.424SerCys: 0.424 ± 0.195
3.462SerAsp: 3.462 ± 0.459
2.049SerGlu: 2.049 ± 0.419
1.625SerPhe: 1.625 ± 0.286
5.44SerGly: 5.44 ± 0.665
0.565SerHis: 0.565 ± 0.203
1.554SerIle: 1.554 ± 0.403
1.908SerLys: 1.908 ± 0.397
4.027SerLeu: 4.027 ± 0.64
1.766SerMet: 1.766 ± 0.314
1.201SerAsn: 1.201 ± 0.275
3.391SerPro: 3.391 ± 0.378
1.908SerGln: 1.908 ± 0.448
2.897SerArg: 2.897 ± 0.523
3.109SerSer: 3.109 ± 0.446
2.967SerThr: 2.967 ± 0.397
3.321SerVal: 3.321 ± 0.443
1.06SerTrp: 1.06 ± 0.265
1.554SerTyr: 1.554 ± 0.328
0.0SerXaa: 0.0 ± 0.0
Thr
8.831ThrAla: 8.831 ± 0.695
0.565ThrCys: 0.565 ± 0.206
4.31ThrAsp: 4.31 ± 0.642
3.674ThrGlu: 3.674 ± 0.552
1.625ThrPhe: 1.625 ± 0.332
6.712ThrGly: 6.712 ± 0.981
0.918ThrHis: 0.918 ± 0.253
3.25ThrIle: 3.25 ± 0.469
2.331ThrLys: 2.331 ± 0.457
5.44ThrLeu: 5.44 ± 0.734
1.13ThrMet: 1.13 ± 0.295
1.837ThrAsn: 1.837 ± 0.343
5.793ThrPro: 5.793 ± 0.594
2.261ThrGln: 2.261 ± 0.389
3.745ThrArg: 3.745 ± 0.504
3.109ThrSer: 3.109 ± 0.54
4.875ThrThr: 4.875 ± 0.755
6.641ThrVal: 6.641 ± 0.961
0.918ThrTrp: 0.918 ± 0.265
1.342ThrTyr: 1.342 ± 0.272
0.0ThrXaa: 0.0 ± 0.0
Val
8.337ValAla: 8.337 ± 0.945
1.06ValCys: 1.06 ± 0.29
4.31ValAsp: 4.31 ± 0.493
5.37ValGlu: 5.37 ± 0.786
1.625ValPhe: 1.625 ± 0.392
5.44ValGly: 5.44 ± 0.689
1.06ValHis: 1.06 ± 0.287
3.321ValIle: 3.321 ± 0.465
2.897ValLys: 2.897 ± 0.532
4.946ValLeu: 4.946 ± 0.529
0.848ValMet: 0.848 ± 0.252
2.755ValAsn: 2.755 ± 0.526
3.391ValPro: 3.391 ± 0.535
2.543ValGln: 2.543 ± 0.361
5.158ValArg: 5.158 ± 0.728
3.533ValSer: 3.533 ± 0.471
5.581ValThr: 5.581 ± 0.637
4.38ValVal: 4.38 ± 0.616
0.918ValTrp: 0.918 ± 0.249
1.201ValTyr: 1.201 ± 0.254
0.0ValXaa: 0.0 ± 0.0
Trp
1.413TrpAla: 1.413 ± 0.314
0.707TrpCys: 0.707 ± 0.267
1.06TrpAsp: 1.06 ± 0.23
0.353TrpGlu: 0.353 ± 0.149
0.424TrpPhe: 0.424 ± 0.18
0.989TrpGly: 0.989 ± 0.211
0.565TrpHis: 0.565 ± 0.197
0.848TrpIle: 0.848 ± 0.255
0.283TrpLys: 0.283 ± 0.161
2.19TrpLeu: 2.19 ± 0.411
0.283TrpMet: 0.283 ± 0.135
0.636TrpAsn: 0.636 ± 0.19
1.13TrpPro: 1.13 ± 0.334
1.06TrpGln: 1.06 ± 0.264
1.484TrpArg: 1.484 ± 0.334
1.272TrpSer: 1.272 ± 0.265
1.696TrpThr: 1.696 ± 0.32
1.554TrpVal: 1.554 ± 0.275
0.707TrpTrp: 0.707 ± 0.215
0.565TrpTyr: 0.565 ± 0.183
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.12TyrAla: 2.12 ± 0.394
0.565TyrCys: 0.565 ± 0.206
2.19TyrAsp: 2.19 ± 0.315
1.272TyrGlu: 1.272 ± 0.232
0.636TyrPhe: 0.636 ± 0.228
2.12TyrGly: 2.12 ± 0.398
0.636TyrHis: 0.636 ± 0.223
0.707TyrIle: 0.707 ± 0.214
0.636TyrLys: 0.636 ± 0.222
1.625TyrLeu: 1.625 ± 0.265
0.353TyrMet: 0.353 ± 0.142
0.777TyrAsn: 0.777 ± 0.237
0.918TyrPro: 0.918 ± 0.256
0.565TyrGln: 0.565 ± 0.189
1.484TyrArg: 1.484 ± 0.299
1.06TyrSer: 1.06 ± 0.268
1.766TyrThr: 1.766 ± 0.381
1.766TyrVal: 1.766 ± 0.487
0.495TyrTrp: 0.495 ± 0.232
0.636TyrTyr: 0.636 ± 0.24
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 72 proteins (14155 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski