Amino acid dipepetide frequency for Mycobacterium phage Pharaoh

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.668AlaAla: 12.668 ± 1.229
0.573AlaCys: 0.573 ± 0.174
5.984AlaAsp: 5.984 ± 0.673
7.193AlaGlu: 7.193 ± 0.748
4.329AlaPhe: 4.329 ± 0.647
8.085AlaGly: 8.085 ± 0.958
1.655AlaHis: 1.655 ± 0.348
5.284AlaIle: 5.284 ± 0.595
4.52AlaLys: 4.52 ± 0.642
8.721AlaLeu: 8.721 ± 1.03
2.928AlaMet: 2.928 ± 0.399
2.992AlaAsn: 2.992 ± 0.465
5.475AlaPro: 5.475 ± 0.98
4.329AlaGln: 4.329 ± 0.748
5.666AlaArg: 5.666 ± 0.586
5.284AlaSer: 5.284 ± 0.564
6.238AlaThr: 6.238 ± 0.673
6.939AlaVal: 6.939 ± 0.667
1.591AlaTrp: 1.591 ± 0.361
2.228AlaTyr: 2.228 ± 0.38
0.0AlaXaa: 0.0 ± 0.0
Cys
0.764CysAla: 0.764 ± 0.192
0.0CysCys: 0.0 ± 0.0
0.637CysAsp: 0.637 ± 0.211
0.382CysGlu: 0.382 ± 0.164
0.382CysPhe: 0.382 ± 0.154
0.637CysGly: 0.637 ± 0.178
0.318CysHis: 0.318 ± 0.167
0.191CysIle: 0.191 ± 0.098
0.255CysLys: 0.255 ± 0.121
0.573CysLeu: 0.573 ± 0.206
0.255CysMet: 0.255 ± 0.133
0.318CysAsn: 0.318 ± 0.129
0.255CysPro: 0.255 ± 0.154
0.064CysGln: 0.064 ± 0.061
0.764CysArg: 0.764 ± 0.263
0.127CysSer: 0.127 ± 0.091
0.509CysThr: 0.509 ± 0.183
0.509CysVal: 0.509 ± 0.161
0.382CysTrp: 0.382 ± 0.162
0.191CysTyr: 0.191 ± 0.103
0.0CysXaa: 0.0 ± 0.0
Asp
5.984AspAla: 5.984 ± 0.603
0.446AspCys: 0.446 ± 0.156
2.61AspAsp: 2.61 ± 0.343
4.074AspGlu: 4.074 ± 0.513
2.355AspPhe: 2.355 ± 0.365
4.965AspGly: 4.965 ± 0.632
1.591AspHis: 1.591 ± 0.41
2.61AspIle: 2.61 ± 0.449
2.61AspLys: 2.61 ± 0.388
5.857AspLeu: 5.857 ± 0.624
1.337AspMet: 1.337 ± 0.26
1.337AspAsn: 1.337 ± 0.293
4.265AspPro: 4.265 ± 0.516
1.973AspGln: 1.973 ± 0.371
2.928AspArg: 2.928 ± 0.454
3.756AspSer: 3.756 ± 0.488
4.52AspThr: 4.52 ± 0.565
3.947AspVal: 3.947 ± 0.624
1.146AspTrp: 1.146 ± 0.25
2.546AspTyr: 2.546 ± 0.309
0.0AspXaa: 0.0 ± 0.0
Glu
6.939GluAla: 6.939 ± 0.721
0.064GluCys: 0.064 ± 0.07
3.756GluAsp: 3.756 ± 0.673
4.329GluGlu: 4.329 ± 0.625
2.483GluPhe: 2.483 ± 0.363
4.52GluGly: 4.52 ± 0.478
1.464GluHis: 1.464 ± 0.368
3.628GluIle: 3.628 ± 0.513
2.228GluLys: 2.228 ± 0.432
5.347GluLeu: 5.347 ± 0.684
2.101GluMet: 2.101 ± 0.355
2.164GluAsn: 2.164 ± 0.266
2.546GluPro: 2.546 ± 0.536
2.419GluGln: 2.419 ± 0.356
4.392GluArg: 4.392 ± 0.678
2.928GluSer: 2.928 ± 0.419
3.374GluThr: 3.374 ± 0.557
4.52GluVal: 4.52 ± 0.52
1.146GluTrp: 1.146 ± 0.261
2.101GluTyr: 2.101 ± 0.312
0.0GluXaa: 0.0 ± 0.0
Phe
3.883PheAla: 3.883 ± 0.423
0.255PheCys: 0.255 ± 0.119
2.037PheAsp: 2.037 ± 0.402
1.973PheGlu: 1.973 ± 0.314
0.7PhePhe: 0.7 ± 0.187
3.756PheGly: 3.756 ± 0.485
0.764PheHis: 0.764 ± 0.198
1.782PheIle: 1.782 ± 0.32
1.528PheLys: 1.528 ± 0.345
2.292PheLeu: 2.292 ± 0.457
0.382PheMet: 0.382 ± 0.145
1.209PheAsn: 1.209 ± 0.262
1.91PhePro: 1.91 ± 0.299
0.955PheGln: 0.955 ± 0.272
1.91PheArg: 1.91 ± 0.259
1.719PheSer: 1.719 ± 0.293
2.737PheThr: 2.737 ± 0.42
2.61PheVal: 2.61 ± 0.438
0.7PheTrp: 0.7 ± 0.217
0.828PheTyr: 0.828 ± 0.218
0.0PheXaa: 0.0 ± 0.0
Gly
7.193GlyAla: 7.193 ± 0.935
0.637GlyCys: 0.637 ± 0.224
6.62GlyAsp: 6.62 ± 0.83
4.647GlyGlu: 4.647 ± 0.64
2.101GlyPhe: 2.101 ± 0.276
8.148GlyGly: 8.148 ± 1.487
1.846GlyHis: 1.846 ± 0.368
3.819GlyIle: 3.819 ± 0.627
3.756GlyLys: 3.756 ± 0.597
6.047GlyLeu: 6.047 ± 0.834
1.655GlyMet: 1.655 ± 0.259
3.119GlyAsn: 3.119 ± 0.453
4.52GlyPro: 4.52 ± 1.072
3.247GlyGln: 3.247 ± 0.626
4.711GlyArg: 4.711 ± 0.501
4.774GlySer: 4.774 ± 0.531
5.22GlyThr: 5.22 ± 0.56
6.62GlyVal: 6.62 ± 0.748
1.846GlyTrp: 1.846 ± 0.275
2.674GlyTyr: 2.674 ± 0.364
0.0GlyXaa: 0.0 ± 0.0
His
2.355HisAla: 2.355 ± 0.417
0.127HisCys: 0.127 ± 0.096
1.019HisAsp: 1.019 ± 0.258
1.082HisGlu: 1.082 ± 0.297
0.764HisPhe: 0.764 ± 0.261
1.655HisGly: 1.655 ± 0.347
0.637HisHis: 0.637 ± 0.204
0.891HisIle: 0.891 ± 0.252
0.891HisLys: 0.891 ± 0.227
1.464HisLeu: 1.464 ± 0.372
0.509HisMet: 0.509 ± 0.188
0.191HisAsn: 0.191 ± 0.11
0.891HisPro: 0.891 ± 0.258
0.828HisGln: 0.828 ± 0.219
1.273HisArg: 1.273 ± 0.314
1.019HisSer: 1.019 ± 0.225
1.4HisThr: 1.4 ± 0.226
1.464HisVal: 1.464 ± 0.345
0.255HisTrp: 0.255 ± 0.149
0.637HisTyr: 0.637 ± 0.283
0.0HisXaa: 0.0 ± 0.0
Ile
5.029IleAla: 5.029 ± 0.557
0.382IleCys: 0.382 ± 0.144
3.374IleAsp: 3.374 ± 0.366
4.774IleGlu: 4.774 ± 0.593
1.591IlePhe: 1.591 ± 0.35
3.438IleGly: 3.438 ± 0.437
0.637IleHis: 0.637 ± 0.203
1.528IleIle: 1.528 ± 0.277
3.056IleLys: 3.056 ± 0.408
3.565IleLeu: 3.565 ± 0.411
0.637IleMet: 0.637 ± 0.175
2.355IleAsn: 2.355 ± 0.353
3.628IlePro: 3.628 ± 0.443
1.528IleGln: 1.528 ± 0.43
3.501IleArg: 3.501 ± 0.456
2.292IleSer: 2.292 ± 0.422
3.692IleThr: 3.692 ± 0.432
2.737IleVal: 2.737 ± 0.41
0.828IleTrp: 0.828 ± 0.244
0.891IleTyr: 0.891 ± 0.228
0.0IleXaa: 0.0 ± 0.0
Lys
5.602LysAla: 5.602 ± 0.691
0.382LysCys: 0.382 ± 0.189
2.037LysAsp: 2.037 ± 0.38
2.228LysGlu: 2.228 ± 0.371
0.891LysPhe: 0.891 ± 0.193
3.883LysGly: 3.883 ± 0.565
0.573LysHis: 0.573 ± 0.208
2.292LysIle: 2.292 ± 0.366
2.355LysLys: 2.355 ± 0.427
4.647LysLeu: 4.647 ± 0.551
0.891LysMet: 0.891 ± 0.225
1.146LysAsn: 1.146 ± 0.247
3.183LysPro: 3.183 ± 0.449
1.528LysGln: 1.528 ± 0.271
2.546LysArg: 2.546 ± 0.369
2.355LysSer: 2.355 ± 0.304
2.737LysThr: 2.737 ± 0.523
2.928LysVal: 2.928 ± 0.41
1.082LysTrp: 1.082 ± 0.23
1.082LysTyr: 1.082 ± 0.262
0.0LysXaa: 0.0 ± 0.0
Leu
9.358LeuAla: 9.358 ± 0.783
0.764LeuCys: 0.764 ± 0.193
4.711LeuAsp: 4.711 ± 0.411
4.265LeuGlu: 4.265 ± 0.604
2.292LeuPhe: 2.292 ± 0.344
5.602LeuGly: 5.602 ± 0.642
1.528LeuHis: 1.528 ± 0.289
4.329LeuIle: 4.329 ± 0.451
3.31LeuLys: 3.31 ± 0.437
5.093LeuLeu: 5.093 ± 0.497
2.355LeuMet: 2.355 ± 0.436
2.355LeuAsn: 2.355 ± 0.502
5.411LeuPro: 5.411 ± 0.588
2.419LeuGln: 2.419 ± 0.486
5.156LeuArg: 5.156 ± 0.737
6.111LeuSer: 6.111 ± 0.584
5.793LeuThr: 5.793 ± 0.59
5.093LeuVal: 5.093 ± 0.532
1.528LeuTrp: 1.528 ± 0.324
2.355LeuTyr: 2.355 ± 0.462
0.0LeuXaa: 0.0 ± 0.0
Met
3.183MetAla: 3.183 ± 0.462
0.064MetCys: 0.064 ± 0.06
1.464MetAsp: 1.464 ± 0.302
1.082MetGlu: 1.082 ± 0.235
0.764MetPhe: 0.764 ± 0.207
1.464MetGly: 1.464 ± 0.311
0.509MetHis: 0.509 ± 0.18
0.955MetIle: 0.955 ± 0.26
1.337MetLys: 1.337 ± 0.294
1.91MetLeu: 1.91 ± 0.378
0.509MetMet: 0.509 ± 0.164
0.955MetAsn: 0.955 ± 0.257
1.273MetPro: 1.273 ± 0.269
1.146MetGln: 1.146 ± 0.238
1.4MetArg: 1.4 ± 0.309
2.355MetSer: 2.355 ± 0.36
2.292MetThr: 2.292 ± 0.267
1.719MetVal: 1.719 ± 0.28
0.7MetTrp: 0.7 ± 0.182
0.573MetTyr: 0.573 ± 0.166
0.0MetXaa: 0.0 ± 0.0
Asn
3.31AsnAla: 3.31 ± 0.595
0.382AsnCys: 0.382 ± 0.168
2.037AsnAsp: 2.037 ± 0.328
1.782AsnGlu: 1.782 ± 0.337
0.446AsnPhe: 0.446 ± 0.19
3.438AsnGly: 3.438 ± 0.59
0.828AsnHis: 0.828 ± 0.187
1.464AsnIle: 1.464 ± 0.26
1.019AsnLys: 1.019 ± 0.268
3.183AsnLeu: 3.183 ± 0.451
1.146AsnMet: 1.146 ± 0.257
0.828AsnAsn: 0.828 ± 0.261
2.483AsnPro: 2.483 ± 0.433
1.019AsnGln: 1.019 ± 0.287
1.655AsnArg: 1.655 ± 0.296
1.464AsnSer: 1.464 ± 0.323
1.528AsnThr: 1.528 ± 0.274
2.61AsnVal: 2.61 ± 0.39
0.573AsnTrp: 0.573 ± 0.173
1.019AsnTyr: 1.019 ± 0.238
0.0AsnXaa: 0.0 ± 0.0
Pro
4.965ProAla: 4.965 ± 0.679
0.382ProCys: 0.382 ± 0.188
3.565ProAsp: 3.565 ± 0.53
4.392ProGlu: 4.392 ± 0.663
2.101ProPhe: 2.101 ± 0.424
4.902ProGly: 4.902 ± 0.714
1.146ProHis: 1.146 ± 0.26
3.374ProIle: 3.374 ± 0.351
2.674ProLys: 2.674 ± 0.506
3.565ProLeu: 3.565 ± 0.498
1.146ProMet: 1.146 ± 0.291
2.419ProAsn: 2.419 ± 0.437
2.546ProPro: 2.546 ± 0.461
2.355ProGln: 2.355 ± 0.492
3.119ProArg: 3.119 ± 0.443
3.056ProSer: 3.056 ± 0.557
5.093ProThr: 5.093 ± 0.59
4.392ProVal: 4.392 ± 0.459
0.828ProTrp: 0.828 ± 0.293
1.655ProTyr: 1.655 ± 0.289
0.0ProXaa: 0.0 ± 0.0
Gln
4.329GlnAla: 4.329 ± 0.603
0.318GlnCys: 0.318 ± 0.156
1.528GlnAsp: 1.528 ± 0.323
1.782GlnGlu: 1.782 ± 0.337
1.273GlnPhe: 1.273 ± 0.242
4.583GlnGly: 4.583 ± 1.544
0.7GlnHis: 0.7 ± 0.242
3.119GlnIle: 3.119 ± 0.44
1.464GlnLys: 1.464 ± 0.322
2.928GlnLeu: 2.928 ± 0.568
1.146GlnMet: 1.146 ± 0.241
1.082GlnAsn: 1.082 ± 0.296
1.655GlnPro: 1.655 ± 0.366
2.164GlnGln: 2.164 ± 0.579
2.037GlnArg: 2.037 ± 0.323
2.292GlnSer: 2.292 ± 0.328
2.355GlnThr: 2.355 ± 0.322
1.91GlnVal: 1.91 ± 0.399
0.637GlnTrp: 0.637 ± 0.196
1.209GlnTyr: 1.209 ± 0.23
0.0GlnXaa: 0.0 ± 0.0
Arg
5.093ArgAla: 5.093 ± 0.563
0.637ArgCys: 0.637 ± 0.232
4.201ArgAsp: 4.201 ± 0.487
3.883ArgGlu: 3.883 ± 0.548
2.483ArgPhe: 2.483 ± 0.508
4.329ArgGly: 4.329 ± 0.566
1.528ArgHis: 1.528 ± 0.346
3.438ArgIle: 3.438 ± 0.473
2.419ArgLys: 2.419 ± 0.385
5.347ArgLeu: 5.347 ± 0.676
2.101ArgMet: 2.101 ± 0.337
2.101ArgAsn: 2.101 ± 0.353
2.992ArgPro: 2.992 ± 0.423
2.355ArgGln: 2.355 ± 0.359
5.284ArgArg: 5.284 ± 0.799
2.737ArgSer: 2.737 ± 0.399
3.438ArgThr: 3.438 ± 0.462
4.138ArgVal: 4.138 ± 0.454
1.337ArgTrp: 1.337 ± 0.256
2.228ArgTyr: 2.228 ± 0.398
0.0ArgXaa: 0.0 ± 0.0
Ser
4.392SerAla: 4.392 ± 0.501
0.764SerCys: 0.764 ± 0.233
3.501SerAsp: 3.501 ± 0.458
4.329SerGlu: 4.329 ± 0.574
2.928SerPhe: 2.928 ± 0.456
5.22SerGly: 5.22 ± 0.682
0.764SerHis: 0.764 ± 0.193
2.037SerIle: 2.037 ± 0.346
2.355SerLys: 2.355 ± 0.463
4.265SerLeu: 4.265 ± 0.535
1.719SerMet: 1.719 ± 0.348
1.146SerAsn: 1.146 ± 0.24
3.438SerPro: 3.438 ± 0.427
1.91SerGln: 1.91 ± 0.361
4.01SerArg: 4.01 ± 0.492
3.247SerSer: 3.247 ± 0.507
2.801SerThr: 2.801 ± 0.337
4.201SerVal: 4.201 ± 0.435
1.464SerTrp: 1.464 ± 0.25
1.464SerTyr: 1.464 ± 0.352
0.0SerXaa: 0.0 ± 0.0
Thr
7.066ThrAla: 7.066 ± 0.662
0.318ThrCys: 0.318 ± 0.142
3.819ThrAsp: 3.819 ± 0.622
2.928ThrGlu: 2.928 ± 0.439
2.419ThrPhe: 2.419 ± 0.297
6.238ThrGly: 6.238 ± 0.743
1.464ThrHis: 1.464 ± 0.325
3.056ThrIle: 3.056 ± 0.521
3.31ThrLys: 3.31 ± 0.51
5.22ThrLeu: 5.22 ± 0.71
1.846ThrMet: 1.846 ± 0.339
1.719ThrAsn: 1.719 ± 0.333
4.774ThrPro: 4.774 ± 0.616
2.865ThrGln: 2.865 ± 0.453
3.501ThrArg: 3.501 ± 0.471
4.074ThrSer: 4.074 ± 0.484
3.692ThrThr: 3.692 ± 0.618
4.583ThrVal: 4.583 ± 0.528
0.891ThrTrp: 0.891 ± 0.262
1.91ThrTyr: 1.91 ± 0.347
0.0ThrXaa: 0.0 ± 0.0
Val
6.748ValAla: 6.748 ± 0.787
0.509ValCys: 0.509 ± 0.221
4.711ValAsp: 4.711 ± 0.541
4.201ValGlu: 4.201 ± 0.519
2.419ValPhe: 2.419 ± 0.438
4.583ValGly: 4.583 ± 0.534
1.019ValHis: 1.019 ± 0.207
2.737ValIle: 2.737 ± 0.407
3.947ValLys: 3.947 ± 0.453
5.666ValLeu: 5.666 ± 0.619
1.528ValMet: 1.528 ± 0.239
2.992ValAsn: 2.992 ± 0.442
3.883ValPro: 3.883 ± 0.342
2.61ValGln: 2.61 ± 0.454
5.22ValArg: 5.22 ± 0.608
3.947ValSer: 3.947 ± 0.479
4.902ValThr: 4.902 ± 0.614
5.857ValVal: 5.857 ± 0.54
1.337ValTrp: 1.337 ± 0.254
1.973ValTyr: 1.973 ± 0.345
0.0ValXaa: 0.0 ± 0.0
Trp
1.973TrpAla: 1.973 ± 0.342
0.382TrpCys: 0.382 ± 0.175
1.273TrpAsp: 1.273 ± 0.277
1.019TrpGlu: 1.019 ± 0.266
0.509TrpPhe: 0.509 ± 0.208
1.082TrpGly: 1.082 ± 0.32
0.255TrpHis: 0.255 ± 0.128
1.273TrpIle: 1.273 ± 0.267
0.764TrpLys: 0.764 ± 0.219
1.209TrpLeu: 1.209 ± 0.245
0.637TrpMet: 0.637 ± 0.184
0.573TrpAsn: 0.573 ± 0.215
1.019TrpPro: 1.019 ± 0.33
1.4TrpGln: 1.4 ± 0.344
1.082TrpArg: 1.082 ± 0.245
0.7TrpSer: 0.7 ± 0.233
1.4TrpThr: 1.4 ± 0.323
1.528TrpVal: 1.528 ± 0.293
0.382TrpTrp: 0.382 ± 0.173
0.446TrpTyr: 0.446 ± 0.176
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.846TyrAla: 1.846 ± 0.323
0.127TyrCys: 0.127 ± 0.1
1.973TyrAsp: 1.973 ± 0.337
2.101TyrGlu: 2.101 ± 0.383
0.7TyrPhe: 0.7 ± 0.193
2.674TyrGly: 2.674 ± 0.415
0.127TyrHis: 0.127 ± 0.085
1.655TyrIle: 1.655 ± 0.219
0.573TyrLys: 0.573 ± 0.222
2.928TyrLeu: 2.928 ± 0.471
0.764TyrMet: 0.764 ± 0.232
1.209TyrAsn: 1.209 ± 0.34
1.528TyrPro: 1.528 ± 0.305
1.4TyrGln: 1.4 ± 0.313
1.91TyrArg: 1.91 ± 0.408
1.719TyrSer: 1.719 ± 0.3
1.973TyrThr: 1.973 ± 0.337
2.483TyrVal: 2.483 ± 0.433
0.318TyrTrp: 0.318 ± 0.16
0.891TyrTyr: 0.891 ± 0.271
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 80 proteins (15710 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski