Klebsiella indica
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4627 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5R9LJ50|A0A5R9LJ50_9ENTR Pyridoxal phosphate homeostasis protein OS=Klebsiella indica OX=2582917 GN=FE839_10735 PE=3 SV=1
MM1 pKa = 7.67 NIGLFYY7 pKa = 10.91 GSSTCYY13 pKa = 10.49 TEE15 pKa = 3.84 MAAEE19 pKa = 5.12 KK20 pKa = 10.0 IRR22 pKa = 11.84 DD23 pKa = 3.65 IIGPEE28 pKa = 4.02 LVTLHH33 pKa = 6.12 NLKK36 pKa = 10.49 DD37 pKa = 3.85 DD38 pKa = 4.22 SPALMTQYY46 pKa = 11.26 DD47 pKa = 4.24 VLILGIPTWDD57 pKa = 3.26 FGEE60 pKa = 4.22 IQEE63 pKa = 4.46 DD64 pKa = 4.11 WEE66 pKa = 4.63 AVWSQLDD73 pKa = 3.73 TLDD76 pKa = 3.79 LQGKK80 pKa = 8.79 IVALYY85 pKa = 11.07 GMGDD89 pKa = 3.29 QLGYY93 pKa = 11.02 GEE95 pKa = 4.87 WFLDD99 pKa = 3.74 ALGMLHH105 pKa = 7.48 DD106 pKa = 4.52 KK107 pKa = 10.74 LATRR111 pKa = 11.84 GVTFIGYY118 pKa = 8.26 WPTEE122 pKa = 4.02 GYY124 pKa = 10.38 EE125 pKa = 4.13 FTSAKK130 pKa = 9.95 PVIADD135 pKa = 3.4 GQLFVGLALDD145 pKa = 3.64 EE146 pKa = 4.38 TNQYY150 pKa = 10.89 DD151 pKa = 4.3 LSDD154 pKa = 3.61 EE155 pKa = 5.41 RR156 pKa = 11.84 IQSWCEE162 pKa = 3.63 QILGEE167 pKa = 4.27 MAEE170 pKa = 4.22 QFAA173 pKa = 4.38
Molecular weight: 19.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.722
IPC2_protein 3.859
IPC_protein 3.834
Toseland 3.63
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.732
Rodwell 3.656
Grimsley 3.541
Solomon 3.795
Lehninger 3.757
Nozaki 3.923
DTASelect 4.126
Thurlkill 3.681
EMBOSS 3.745
Sillero 3.948
Patrickios 1.036
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.826
Protein with the highest isoelectric point:
>tr|A0A5R9LHW8|A0A5R9LHW8_9ENTR Transposase (Fragment) OS=Klebsiella indica OX=2582917 GN=FE839_12520 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 NRR14 pKa = 11.84 SHH16 pKa = 7.16 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.22 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LTVSKK46 pKa = 10.99
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4627
0
4627
1489319
14
1840
321.9
35.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.974 ± 0.041
1.139 ± 0.012
5.087 ± 0.028
5.414 ± 0.033
3.881 ± 0.025
7.537 ± 0.033
2.326 ± 0.019
6.006 ± 0.032
4.002 ± 0.031
11.016 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.781 ± 0.018
3.634 ± 0.026
4.528 ± 0.019
4.423 ± 0.028
5.85 ± 0.032
5.895 ± 0.024
5.216 ± 0.025
7.012 ± 0.029
1.537 ± 0.016
2.743 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here