Chlamydia trachomatis (strain D/UW-3/Cx)
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 895 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|O84808|RS18_CHLTR 30S ribosomal protein S18 OS=Chlamydia trachomatis (strain D/UW-3/Cx) OX=272561 GN=rpsR PE=3 SV=1
MM1 pKa = 7.86 RR2 pKa = 11.84 FLLALFSLILVLPATEE18 pKa = 4.36 AFSTEE23 pKa = 4.43 DD24 pKa = 3.36 KK25 pKa = 10.85 QCQQEE30 pKa = 4.14 AEE32 pKa = 4.2 EE33 pKa = 5.04 DD34 pKa = 3.9 CSQVADD40 pKa = 3.51 TCVFYY45 pKa = 10.94 SYY47 pKa = 11.85 AEE49 pKa = 4.24 GLEE52 pKa = 4.03 HH53 pKa = 7.41 ARR55 pKa = 11.84 DD56 pKa = 3.67 EE57 pKa = 4.74 GKK59 pKa = 8.63 LTLVVLLDD67 pKa = 3.32 TSGYY71 pKa = 10.38 SFEE74 pKa = 4.34 TLADD78 pKa = 3.5 AAHH81 pKa = 6.39 AMEE84 pKa = 5.18 SSLLSTFADD93 pKa = 3.88 FVVLSRR99 pKa = 11.84 RR100 pKa = 11.84 EE101 pKa = 4.35 AVPLIYY107 pKa = 10.28 PPVPDD112 pKa = 4.14 PMVGEE117 pKa = 3.71 IALFLEE123 pKa = 4.72 AFSDD127 pKa = 3.72 QTFPSQPVIVTLAIGASSAEE147 pKa = 3.83 IMDD150 pKa = 3.33 ITEE153 pKa = 4.24 IPSINPEE160 pKa = 3.83 FVEE163 pKa = 4.33
Molecular weight: 17.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.768
IPC2_protein 3.872
IPC_protein 3.808
Toseland 3.63
ProMoST 3.923
Dawson 3.77
Bjellqvist 3.973
Wikipedia 3.668
Rodwell 3.643
Grimsley 3.541
Solomon 3.757
Lehninger 3.719
Nozaki 3.897
DTASelect 4.037
Thurlkill 3.668
EMBOSS 3.681
Sillero 3.923
Patrickios 1.85
IPC_peptide 3.77
IPC2_peptide 3.91
IPC2.peptide.svr19 3.848
Protein with the highest isoelectric point:
>tr|O84803|O84803_CHLTR CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase OS=Chlamydia trachomatis (strain D/UW-3/Cx) OX=272561 GN=pgsA_2 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.29 QPSKK9 pKa = 9.17 RR10 pKa = 11.84 KK11 pKa = 9.43 RR12 pKa = 11.84 RR13 pKa = 11.84 NSVGFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.34 SGRR28 pKa = 11.84 NLLNRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 HH37 pKa = 5.26 GRR39 pKa = 11.84 HH40 pKa = 5.61 SLIDD44 pKa = 3.51 LL45 pKa = 4.14
Molecular weight: 5.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 10.891
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.954
Rodwell 12.135
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.871
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.101
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
895
0
895
312168
45
1786
348.8
38.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.532 ± 0.066
1.626 ± 0.032
4.524 ± 0.059
6.613 ± 0.076
4.829 ± 0.061
6.347 ± 0.084
2.304 ± 0.044
6.607 ± 0.062
5.755 ± 0.07
11.226 ± 0.093
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.051 ± 0.028
3.502 ± 0.064
4.369 ± 0.058
4.193 ± 0.058
4.838 ± 0.067
8.117 ± 0.093
5.12 ± 0.082
6.421 ± 0.066
0.956 ± 0.029
3.068 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here