Rhodococcus phage REQ3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G9FH64|G9FH64_9CAUD Uncharacterized protein OS=Rhodococcus phage REQ3 OX=1109714 PE=4 SV=1
MM1 pKa = 6.38QTFIEE6 pKa = 4.33RR7 pKa = 11.84GEE9 pKa = 4.38VIALDD14 pKa = 4.65CSHH17 pKa = 6.92QDD19 pKa = 2.97TILFYY24 pKa = 10.84DD25 pKa = 4.34PSYY28 pKa = 11.4GPYY31 pKa = 8.99EE32 pKa = 4.1KK33 pKa = 10.44CVEE36 pKa = 4.23CEE38 pKa = 4.15VILDD42 pKa = 4.19EE43 pKa = 5.4EE44 pKa = 4.9DD45 pKa = 3.9ALADD49 pKa = 3.57NDD51 pKa = 4.05LTDD54 pKa = 3.89EE55 pKa = 4.49EE56 pKa = 5.04VVADD60 pKa = 3.88AGVWVAIWLSALAWTAVVGLGLVWWAVTAA89 pKa = 4.27

Molecular weight: 9.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G9FH49|G9FH49_9CAUD Uncharacterized protein OS=Rhodococcus phage REQ3 OX=1109714 PE=4 SV=1
MM1 pKa = 7.77PRR3 pKa = 11.84PSLPVGAHH11 pKa = 4.91GRR13 pKa = 11.84ISRR16 pKa = 11.84TKK18 pKa = 10.64LPDD21 pKa = 3.5GRR23 pKa = 11.84WRR25 pKa = 11.84AACRR29 pKa = 11.84FRR31 pKa = 11.84DD32 pKa = 3.44ADD34 pKa = 3.55GVTRR38 pKa = 11.84QVVRR42 pKa = 11.84YY43 pKa = 6.86TPPTVDD49 pKa = 3.46RR50 pKa = 11.84DD51 pKa = 3.71KK52 pKa = 11.25TGAAAEE58 pKa = 4.13RR59 pKa = 11.84ALVDD63 pKa = 3.61ALKK66 pKa = 10.83GRR68 pKa = 11.84STTGDD73 pKa = 3.49LSADD77 pKa = 3.49SRR79 pKa = 11.84VSEE82 pKa = 3.76LWMAYY87 pKa = 9.71RR88 pKa = 11.84AQLEE92 pKa = 4.41EE93 pKa = 4.51KK94 pKa = 10.34NRR96 pKa = 11.84SQSTLQDD103 pKa = 3.73YY104 pKa = 11.32DD105 pKa = 4.06RR106 pKa = 11.84MAAKK110 pKa = 10.21ILDD113 pKa = 3.68GLGNLRR119 pKa = 11.84VRR121 pKa = 11.84EE122 pKa = 4.21ATTQRR127 pKa = 11.84LDD129 pKa = 3.31TFVRR133 pKa = 11.84EE134 pKa = 3.55IATRR138 pKa = 11.84QGAGTGKK145 pKa = 10.0KK146 pKa = 10.25AKK148 pKa = 9.47TILSGMFRR156 pKa = 11.84IAVRR160 pKa = 11.84YY161 pKa = 8.89GAVQANPVRR170 pKa = 11.84EE171 pKa = 4.25VTDD174 pKa = 4.0LGAGRR179 pKa = 11.84KK180 pKa = 8.82KK181 pKa = 10.09RR182 pKa = 11.84AKK184 pKa = 10.85SMDD187 pKa = 3.56RR188 pKa = 11.84EE189 pKa = 4.25LLVQLLADD197 pKa = 3.82VRR199 pKa = 11.84GSEE202 pKa = 4.35APCPVVLSEE211 pKa = 4.04AQIKK215 pKa = 10.48RR216 pKa = 11.84GVKK219 pKa = 6.35TTSKK223 pKa = 10.25AGQVPSVAQFCQAADD238 pKa = 3.71LADD241 pKa = 5.16LIVMFAATGARR252 pKa = 11.84IGEE255 pKa = 4.07VLGIRR260 pKa = 11.84WEE262 pKa = 4.19DD263 pKa = 2.95VDD265 pKa = 4.3LKK267 pKa = 11.06KK268 pKa = 10.31RR269 pKa = 11.84TVAIAGKK276 pKa = 8.74VIRR279 pKa = 11.84VKK281 pKa = 11.01GDD283 pKa = 3.11GLVRR287 pKa = 11.84EE288 pKa = 5.08DD289 pKa = 3.47STKK292 pKa = 10.23TEE294 pKa = 3.74SGLRR298 pKa = 11.84QLPLPGFAVEE308 pKa = 4.12MLEE311 pKa = 4.21KK312 pKa = 10.53RR313 pKa = 11.84LVDD316 pKa = 3.22RR317 pKa = 11.84TGPMVFPSKK326 pKa = 10.74VGTLRR331 pKa = 11.84DD332 pKa = 3.52PDD334 pKa = 3.81TVQRR338 pKa = 11.84QWRR341 pKa = 11.84QVRR344 pKa = 11.84AALDD348 pKa = 3.99LEE350 pKa = 4.58WVTTHH355 pKa = 6.35TFRR358 pKa = 11.84KK359 pKa = 7.48TVATILDD366 pKa = 4.19DD367 pKa = 4.23EE368 pKa = 4.9GLTARR373 pKa = 11.84QAADD377 pKa = 3.45HH378 pKa = 6.79LGHH381 pKa = 6.68AQVSMTQDD389 pKa = 2.98VYY391 pKa = 11.15LGRR394 pKa = 11.84GRR396 pKa = 11.84THH398 pKa = 6.46SAAAAALDD406 pKa = 3.57AAVAKK411 pKa = 10.48RR412 pKa = 3.45

Molecular weight: 45.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

12346

40

1585

205.8

22.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.765 ± 0.488

0.923 ± 0.205

6.707 ± 0.276

6.14 ± 0.356

2.576 ± 0.178

8.57 ± 0.562

1.96 ± 0.212

4.674 ± 0.245

3.532 ± 0.321

7.614 ± 0.241

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.236 ± 0.137

3.005 ± 0.191

5.435 ± 0.337

3.515 ± 0.231

7.079 ± 0.478

5.613 ± 0.251

7.031 ± 0.33

6.699 ± 0.327

1.717 ± 0.15

2.211 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski