Nonlabens spongiae

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Nonlabens

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2989 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6MPD0|A0A1W6MPD0_9FLAO Uncharacterized protein OS=Nonlabens spongiae OX=331648 GN=BST97_11475 PE=4 SV=1
MM1 pKa = 7.25MNKK4 pKa = 9.81FYY6 pKa = 9.79ITLITVSVFTAQSWAQVPSNYY27 pKa = 9.46YY28 pKa = 10.71DD29 pKa = 3.31SAAGLTGYY37 pKa = 10.26ALKK40 pKa = 10.84SEE42 pKa = 4.28LANIISANYY51 pKa = 8.44NAQSYY56 pKa = 11.48DD57 pKa = 3.7DD58 pKa = 5.65LRR60 pKa = 11.84DD61 pKa = 3.62LYY63 pKa = 11.19AISDD67 pKa = 3.48NDD69 pKa = 3.45AYY71 pKa = 11.3YY72 pKa = 11.09DD73 pKa = 3.62NGQQTTTILDD83 pKa = 4.26LYY85 pKa = 10.98SEE87 pKa = 4.72NPNGADD93 pKa = 3.65PYY95 pKa = 10.53TFSATNTNDD104 pKa = 2.96RR105 pKa = 11.84CGNYY109 pKa = 9.88SGEE112 pKa = 3.96GDD114 pKa = 3.58CWNRR118 pKa = 11.84EE119 pKa = 4.08HH120 pKa = 7.55IFPQGFFNQLEE131 pKa = 4.28PMRR134 pKa = 11.84SDD136 pKa = 2.84AHH138 pKa = 6.83HH139 pKa = 6.94VIPTDD144 pKa = 3.37GFVNGGRR151 pKa = 11.84SNLPFGEE158 pKa = 4.06VDD160 pKa = 3.6LSGSGIRR167 pKa = 11.84TYY169 pKa = 11.31QNGSRR174 pKa = 11.84KK175 pKa = 9.98GPSATPGYY183 pKa = 8.62TGDD186 pKa = 3.36VFEE189 pKa = 6.24PIDD192 pKa = 3.88EE193 pKa = 4.55FKK195 pKa = 11.47GDD197 pKa = 3.59IARR200 pKa = 11.84MLLYY204 pKa = 10.32FATRR208 pKa = 11.84YY209 pKa = 8.99EE210 pKa = 4.97DD211 pKa = 3.47RR212 pKa = 11.84FNDD215 pKa = 4.13NRR217 pKa = 11.84WDD219 pKa = 3.91SPNATNDD226 pKa = 3.47PRR228 pKa = 11.84DD229 pKa = 3.5GSQDD233 pKa = 2.94QYY235 pKa = 11.45YY236 pKa = 8.05EE237 pKa = 3.45QWYY240 pKa = 9.99IDD242 pKa = 5.18LLLSWHH248 pKa = 6.37AQDD251 pKa = 4.37PVSQRR256 pKa = 11.84EE257 pKa = 3.42IDD259 pKa = 3.9RR260 pKa = 11.84NNDD263 pKa = 2.35IYY265 pKa = 11.59NFQNNANPYY274 pKa = 8.63IDD276 pKa = 4.42NPQFVDD282 pKa = 4.37MIWNSSQAPSGSIFATLTDD301 pKa = 3.72SYY303 pKa = 11.8NDD305 pKa = 3.7VNSNGYY311 pKa = 9.58DD312 pKa = 3.11AGDD315 pKa = 4.32EE316 pKa = 4.04INYY319 pKa = 10.45DD320 pKa = 3.55YY321 pKa = 11.06TIEE324 pKa = 4.11NLGNTTLYY332 pKa = 10.71NVTVTASRR340 pKa = 11.84GNFANTVSPIASIAPGQVINNPFGNLQVVLITQDD374 pKa = 2.9VDD376 pKa = 3.69PNGACDD382 pKa = 5.05VINQLQVTADD392 pKa = 4.2FSANEE397 pKa = 4.06NTGGLSIASDD407 pKa = 4.08DD408 pKa = 3.94PDD410 pKa = 3.52NFQDD414 pKa = 4.15VDD416 pKa = 4.49SNNDD420 pKa = 3.56NLPDD424 pKa = 4.82DD425 pKa = 3.95PTISNVCGGSTGTVSEE441 pKa = 4.68LFISEE446 pKa = 4.47YY447 pKa = 10.45IEE449 pKa = 4.63GSGSNKK455 pKa = 10.16AIEE458 pKa = 4.14IANFTGSQVNLSGYY472 pKa = 9.7SIEE475 pKa = 4.54RR476 pKa = 11.84NANGGSTWSGTISLSGTLDD495 pKa = 3.19NGEE498 pKa = 4.4VYY500 pKa = 10.89VLARR504 pKa = 11.84GNADD508 pKa = 3.34QAILDD513 pKa = 3.88EE514 pKa = 4.59ADD516 pKa = 3.61RR517 pKa = 11.84LIGNGNALDD526 pKa = 4.16FNGNDD531 pKa = 3.41PVGLFRR537 pKa = 11.84NGNLIDD543 pKa = 3.48IVGVFNSGSTDD554 pKa = 3.65FAKK557 pKa = 10.8DD558 pKa = 3.32VVLVRR563 pKa = 11.84KK564 pKa = 9.41PDD566 pKa = 3.52AVVPNLDD573 pKa = 3.77FNLTRR578 pKa = 11.84DD579 pKa = 3.5WNSFGQSNYY588 pKa = 9.46TDD590 pKa = 4.43LGQHH594 pKa = 5.37TVTTASEE601 pKa = 4.06DD602 pKa = 3.52QLIAEE607 pKa = 4.53SFKK610 pKa = 11.2VYY612 pKa = 10.36PNPSKK617 pKa = 11.08DD618 pKa = 3.17GIFYY622 pKa = 10.86FEE624 pKa = 4.28TDD626 pKa = 3.41LEE628 pKa = 4.41EE629 pKa = 5.69IEE631 pKa = 5.66LNVFDD636 pKa = 5.44LSGRR640 pKa = 11.84SISFDD645 pKa = 3.15QTQDD649 pKa = 3.68SIQLEE654 pKa = 4.03QAGIYY659 pKa = 9.92ILTVEE664 pKa = 4.2KK665 pKa = 10.83DD666 pKa = 3.63GNRR669 pKa = 11.84SSLKK673 pKa = 10.47LVLRR677 pKa = 4.67

Molecular weight: 74.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6MJN6|A0A1W6MJN6_9FLAO DUF4835 domain-containing protein OS=Nonlabens spongiae OX=331648 GN=BST97_07335 PE=4 SV=1
MM1 pKa = 6.94AQKK4 pKa = 9.31RR5 pKa = 11.84TYY7 pKa = 10.22QPSKK11 pKa = 9.01RR12 pKa = 11.84KK13 pKa = 9.47RR14 pKa = 11.84RR15 pKa = 11.84NKK17 pKa = 9.49HH18 pKa = 3.94GFRR21 pKa = 11.84EE22 pKa = 4.27RR23 pKa = 11.84MASANGRR30 pKa = 11.84KK31 pKa = 9.04VLARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84AKK39 pKa = 10.09GRR41 pKa = 11.84KK42 pKa = 8.0KK43 pKa = 10.66LSVSTEE49 pKa = 3.69RR50 pKa = 11.84RR51 pKa = 11.84HH52 pKa = 6.27KK53 pKa = 10.62RR54 pKa = 3.14

Molecular weight: 6.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2989

0

2989

982028

38

3707

328.5

37.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.606 ± 0.045

0.744 ± 0.013

6.232 ± 0.052

6.821 ± 0.047

4.977 ± 0.036

6.485 ± 0.055

1.847 ± 0.025

7.349 ± 0.038

6.882 ± 0.073

9.319 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.33 ± 0.027

5.589 ± 0.051

3.428 ± 0.024

3.715 ± 0.029

4.158 ± 0.037

6.577 ± 0.042

5.565 ± 0.05

6.375 ± 0.035

1.037 ± 0.017

3.966 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski