Actinobacillus pleuropneumoniae serotype 5b (strain L20)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Actinobacillus; Actinobacillus pleuropneumoniae; Actinobacillus pleuropneumoniae serovar 5

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2004 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A3N2C5|A3N2C5_ACTP2 Uncharacterized protein OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) OX=416269 GN=APL_1477 PE=4 SV=1
MM1 pKa = 7.5SNIQIPLIFTDD12 pKa = 4.01AAAKK16 pKa = 9.64KK17 pKa = 9.6VKK19 pKa = 10.5SLIEE23 pKa = 4.49GEE25 pKa = 4.46DD26 pKa = 3.6NPNLRR31 pKa = 11.84LRR33 pKa = 11.84VYY35 pKa = 9.12ITGGGCSGFQYY46 pKa = 11.03GFTFDD51 pKa = 4.33DD52 pKa = 4.34QINEE56 pKa = 3.85GDD58 pKa = 3.83LTIEE62 pKa = 4.05NQNVGLVVDD71 pKa = 4.64PMSLQYY77 pKa = 11.06LIGGTVDD84 pKa = 3.33YY85 pKa = 11.24TEE87 pKa = 5.04GLDD90 pKa = 3.38GSRR93 pKa = 11.84FVVQNPNASSTCGCGSSFSII113 pKa = 5.02

Molecular weight: 12.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|A3N3P3|PROA_ACTP2 Gamma-glutamyl phosphate reductase OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) OX=416269 GN=proA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.15GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2004

0

2004

651114

37

3347

324.9

36.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.773 ± 0.062

0.977 ± 0.019

4.96 ± 0.043

6.432 ± 0.062

4.466 ± 0.039

6.775 ± 0.072

2.028 ± 0.027

6.76 ± 0.048

6.223 ± 0.047

10.466 ± 0.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.393 ± 0.028

4.687 ± 0.06

3.723 ± 0.036

4.809 ± 0.054

4.442 ± 0.051

5.764 ± 0.045

5.237 ± 0.061

6.801 ± 0.055

1.147 ± 0.018

3.138 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski