Koribacter versatilis (strain Ellin345)
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4771 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q1IMF6|Q1IMF6_KORVE Polysulphide reductase NrfD OS=Koribacter versatilis (strain Ellin345) OX=204669 GN=Acid345_2943 PE=3 SV=1
MM1 pKa = 7.23 RR2 pKa = 11.84 QVAAVSFFLVFTAVFSAYY20 pKa = 10.59 SQDD23 pKa = 3.02 LTQAKK28 pKa = 7.43 RR29 pKa = 11.84 TPAASGSTQPNVFLTPKK46 pKa = 9.65 QYY48 pKa = 10.08 PAGPSGVTSIAKK60 pKa = 10.23 GDD62 pKa = 3.95 FNNDD66 pKa = 2.2 SYY68 pKa = 11.77 MDD70 pKa = 3.59 VAVTNVSGTITVLLGKK86 pKa = 10.62 GDD88 pKa = 3.9 GTFQAPVSYY97 pKa = 9.09 PALSSPVSIAAADD110 pKa = 3.87 LNGDD114 pKa = 3.83 GKK116 pKa = 11.32 LDD118 pKa = 3.73 LAVANSGSGSISVFLGNGDD137 pKa = 3.67 GTFQSHH143 pKa = 5.6 TDD145 pKa = 3.47 VAVGTSVQMLTVADD159 pKa = 4.33 FNGDD163 pKa = 3.82 GKK165 pKa = 10.11 PDD167 pKa = 3.66 LAVLVDD173 pKa = 3.62 GMVSVLIGKK182 pKa = 9.88 GDD184 pKa = 3.42 ATFNAIGEE192 pKa = 4.39 YY193 pKa = 10.51 AKK195 pKa = 10.74 PCATYY200 pKa = 10.68 LATGDD205 pKa = 3.8 FNGDD209 pKa = 3.04 GKK211 pKa = 9.5 TDD213 pKa = 3.18 IVAGRR218 pKa = 11.84 QCVLLGNGDD227 pKa = 3.82 GTFQPPVGSQKK238 pKa = 10.23 IGNTVSTAVGDD249 pKa = 3.75 INGDD253 pKa = 3.42 GKK255 pKa = 11.3 LDD257 pKa = 4.71 LIEE260 pKa = 5.21 GGIGDD265 pKa = 3.73 SDD267 pKa = 3.69 GTPRR271 pKa = 11.84 ALVVVLLGNGDD282 pKa = 3.81 GTFQPPQGFFGYY294 pKa = 10.62 GSGVQGLLLADD305 pKa = 3.96 VNGDD309 pKa = 3.45 SHH311 pKa = 8.07 PDD313 pKa = 3.04 IVLSSSEE320 pKa = 3.88 NVEE323 pKa = 4.25 VVNGKK328 pKa = 10.39 GDD330 pKa = 3.62 GTFEE334 pKa = 4.62 PGVLYY339 pKa = 9.87 PVGNRR344 pKa = 11.84 PVAGGLVLSDD354 pKa = 3.25 FTGSGRR360 pKa = 11.84 LDD362 pKa = 3.54 LAVLTSCANPSICGDD377 pKa = 3.65 GAVTLLRR384 pKa = 11.84 GKK386 pKa = 11.1 GDD388 pKa = 3.53 GTYY391 pKa = 10.17 VAPASYY397 pKa = 10.83 YY398 pKa = 9.91 IFEE401 pKa = 4.3 GDD403 pKa = 3.29 EE404 pKa = 4.0 RR405 pKa = 11.84 FDD407 pKa = 4.82 AVGGWVAVGDD417 pKa = 3.82 FDD419 pKa = 6.4 GDD421 pKa = 3.63 GKK423 pKa = 11.35 LDD425 pKa = 3.53 VLEE428 pKa = 4.66 VFDD431 pKa = 3.82 TRR433 pKa = 11.84 AFISLGNGDD442 pKa = 3.78 GTVQTGQRR450 pKa = 11.84 YY451 pKa = 7.86 YY452 pKa = 10.65 QVAYY456 pKa = 10.24 QSDD459 pKa = 4.2 GAVVGDD465 pKa = 3.76 FNGDD469 pKa = 3.33 GKK471 pKa = 11.22 LDD473 pKa = 3.54 AAILHH478 pKa = 6.2 SCDD481 pKa = 3.38 VFYY484 pKa = 11.14 SDD486 pKa = 5.35 PGPGNPPPYY495 pKa = 9.24 CVSAGSVGVLLGNGNGTWQRR515 pKa = 11.84 WPEE518 pKa = 3.8 SLYY521 pKa = 10.94 FGVGDD526 pKa = 4.18 TPTSIATGDD535 pKa = 3.56 FNQDD539 pKa = 2.46 GKK541 pKa = 11.51 LDD543 pKa = 3.94 LVVSDD548 pKa = 4.54 GANAYY553 pKa = 9.35 ILLGNGDD560 pKa = 3.64 GTFPVHH566 pKa = 5.63 QAYY569 pKa = 7.26 PTGAAADD576 pKa = 3.97 YY577 pKa = 10.66 LNPFLPNAQSVVVGDD592 pKa = 4.12 FNGDD596 pKa = 3.53 GAPDD600 pKa = 3.59 VAVSNSDD607 pKa = 2.9 GGIAVLLGNGDD618 pKa = 3.53 GTLRR622 pKa = 11.84 APQLFPAIKK631 pKa = 9.93 SSQSLAIGDD640 pKa = 4.04 LNRR643 pKa = 11.84 DD644 pKa = 3.17 GKK646 pKa = 11.13 LDD648 pKa = 3.41 IVASDD653 pKa = 3.79 GSGSISIFLGNGDD666 pKa = 3.87 GTFQTSKK673 pKa = 10.49 VYY675 pKa = 10.57 AAVGSQSVTVGDD687 pKa = 4.02 FNGDD691 pKa = 3.64 GILDD695 pKa = 3.78 VASGTGHH702 pKa = 5.69 TVSLLLGNGDD712 pKa = 4.23 GSLQPPVNYY721 pKa = 9.74 IVGLSATGLAAGDD734 pKa = 3.98 FNGDD738 pKa = 3.17 GALDD742 pKa = 3.91 LVTEE746 pKa = 4.75 DD747 pKa = 5.85 FSILLNRR754 pKa = 11.84 QGTQLNVQSSRR765 pKa = 11.84 NPSNVGQPVTFTVTSAASLPEE786 pKa = 3.98 TGLPSGTITLRR797 pKa = 11.84 DD798 pKa = 3.42 GSTVLGEE805 pKa = 4.24 SGVSGVFDD813 pKa = 3.68 VKK815 pKa = 11.16 VSGLTAGTHH824 pKa = 5.91 QITATYY830 pKa = 10.46 SGDD833 pKa = 3.73 NNFQPHH839 pKa = 4.7 TTAILTEE846 pKa = 4.45 HH847 pKa = 6.35 VGAPATMISPALGSILTSTTVTFAWKK873 pKa = 9.6 AAAGASQYY881 pKa = 11.61 SLYY884 pKa = 10.85 LGTKK888 pKa = 9.4 PGRR891 pKa = 11.84 DD892 pKa = 3.28 DD893 pKa = 3.79 LGYY896 pKa = 11.14 VNAHH900 pKa = 6.42 SSTSATVKK908 pKa = 10.37 NLPSTGSSLYY918 pKa = 8.75 VTLFSLVGGVYY929 pKa = 10.29 YY930 pKa = 10.86 SNSYY934 pKa = 9.58 TYY936 pKa = 10.48 ILPGTPAKK944 pKa = 10.9 AKK946 pKa = 7.3 MTSPLPGTMLVGKK959 pKa = 9.61 DD960 pKa = 3.36 ATFTWSHH967 pKa = 5.34 GTGVTYY973 pKa = 10.74 YY974 pKa = 10.6 SLYY977 pKa = 10.9 VGTKK981 pKa = 10.14 GYY983 pKa = 7.77 GTHH986 pKa = 7.45 DD987 pKa = 4.19 LDD989 pKa = 5.68 FINATTTSASVSNLPADD1006 pKa = 3.85 GSTIYY1011 pKa = 10.8 VQVNSYY1017 pKa = 10.61 IDD1019 pKa = 3.86 GAWTSQSYY1027 pKa = 8.68 TYY1029 pKa = 10.39 ISGSGTPAPATMISPTPGSSISGNSATFTWTSGVGVSEE1067 pKa = 4.32 FSLYY1071 pKa = 10.9 VGTGGVGSHH1080 pKa = 6.79 NIAFIEE1086 pKa = 4.25 TGTTSATVTGLPATGATIYY1105 pKa = 10.7 VRR1107 pKa = 11.84 LNSFVNGAWQWVDD1120 pKa = 2.79 YY1121 pKa = 10.71 SYY1123 pKa = 11.62 RR1124 pKa = 11.84 NPP1126 pKa = 3.45
Molecular weight: 114.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.819
IPC2_protein 4.05
IPC_protein 4.113
Toseland 3.872
ProMoST 4.291
Dawson 4.126
Bjellqvist 4.279
Wikipedia 4.088
Rodwell 3.935
Grimsley 3.77
Solomon 4.126
Lehninger 4.075
Nozaki 4.228
DTASelect 4.558
Thurlkill 3.923
EMBOSS 4.101
Sillero 4.24
Patrickios 1.43
IPC_peptide 4.113
IPC2_peptide 4.215
IPC2.peptide.svr19 4.104
Protein with the highest isoelectric point:
>tr|Q1IV87|Q1IV87_KORVE Uncharacterized protein OS=Koribacter versatilis (strain Ellin345) OX=204669 GN=Acid345_0208 PE=4 SV=1
MM1 pKa = 8.0 PKK3 pKa = 9.02 RR4 pKa = 11.84 TFQPNRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 SKK15 pKa = 8.4 THH17 pKa = 5.66 GFRR20 pKa = 11.84 TRR22 pKa = 11.84 MKK24 pKa = 8.81 TKK26 pKa = 10.13 SGAAVLSRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.64 GRR40 pKa = 11.84 KK41 pKa = 8.44 RR42 pKa = 11.84 VSVSAGYY49 pKa = 10.39 RR50 pKa = 11.84 DD51 pKa = 3.36
Molecular weight: 5.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.373
IPC2_protein 10.935
IPC_protein 12.369
Toseland 12.53
ProMoST 13.027
Dawson 12.53
Bjellqvist 12.53
Wikipedia 13.013
Rodwell 12.223
Grimsley 12.574
Solomon 13.027
Lehninger 12.925
Nozaki 12.53
DTASelect 12.53
Thurlkill 12.53
EMBOSS 13.027
Sillero 12.53
Patrickios 11.959
IPC_peptide 13.027
IPC2_peptide 12.018
IPC2.peptide.svr19 9.083
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4771
0
4771
1677413
37
3121
351.6
38.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.494 ± 0.04
0.903 ± 0.012
5.257 ± 0.027
5.713 ± 0.042
4.053 ± 0.023
7.895 ± 0.039
2.242 ± 0.018
5.205 ± 0.027
4.473 ± 0.029
9.353 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.341 ± 0.018
3.542 ± 0.032
5.016 ± 0.025
3.735 ± 0.022
5.969 ± 0.04
6.252 ± 0.03
5.872 ± 0.038
7.52 ± 0.03
1.359 ± 0.017
2.808 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here