Thermoplasmatales archaeon SCGC AB-540-F20

Taxonomy: cellular organisms; Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Thermoplasmatales; unclassified Thermoplasmatales

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1255 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M7TFT6|M7TFT6_9ARCH Thioredoxin OS=Thermoplasmatales archaeon SCGC AB-540-F20 OX=1242866 GN=MBGDF03_00437 PE=3 SV=1
MM1 pKa = 7.48KK2 pKa = 10.31KK3 pKa = 9.78IISSILVILFVLAGLEE19 pKa = 4.25VIGISNDD26 pKa = 2.89KK27 pKa = 10.17TIEE30 pKa = 3.92NFYY33 pKa = 11.09EE34 pKa = 4.3SDD36 pKa = 3.54FKK38 pKa = 11.72GLFDD42 pKa = 5.91DD43 pKa = 3.99ISVYY47 pKa = 10.16RR48 pKa = 11.84YY49 pKa = 7.52QTACQGKK56 pKa = 8.73VPFSMWSIDD65 pKa = 3.44ALYY68 pKa = 10.37TNSEE72 pKa = 4.17NVNEE76 pKa = 4.06NLYY79 pKa = 10.71FEE81 pKa = 4.97QIHH84 pKa = 6.67SIGFDD89 pKa = 2.99RR90 pKa = 11.84DD91 pKa = 3.64YY92 pKa = 11.16PIGEE96 pKa = 4.91IIWQYY101 pKa = 11.54LLTIYY106 pKa = 10.58DD107 pKa = 4.46PSPKK111 pKa = 10.08AIAPIEE117 pKa = 4.84DD118 pKa = 3.55INGDD122 pKa = 3.96GISDD126 pKa = 4.09VIVCSEE132 pKa = 4.44DD133 pKa = 3.12DD134 pKa = 3.36HH135 pKa = 7.27VRR137 pKa = 11.84CFGGGAIGTGVVLWGHH153 pKa = 6.73EE154 pKa = 3.99IYY156 pKa = 10.97AGDD159 pKa = 3.92VYY161 pKa = 11.12NQNGLDD167 pKa = 4.12IIDD170 pKa = 4.94DD171 pKa = 3.83VDD173 pKa = 4.14GDD175 pKa = 4.04GYY177 pKa = 11.31EE178 pKa = 4.14DD179 pKa = 3.99VVVGAAWGARR189 pKa = 11.84LIRR192 pKa = 11.84CISGIDD198 pKa = 3.7GSTIWTHH205 pKa = 5.82DD206 pKa = 3.06THH208 pKa = 7.03EE209 pKa = 4.42YY210 pKa = 10.96GGGGWVYY217 pKa = 9.96MVNCSYY223 pKa = 11.21DD224 pKa = 3.45YY225 pKa = 11.52DD226 pKa = 5.01GDD228 pKa = 4.14GVKK231 pKa = 10.51DD232 pKa = 3.7VLATCGDD239 pKa = 4.09DD240 pKa = 5.19SSDD243 pKa = 3.43TGPKK247 pKa = 9.28RR248 pKa = 11.84VYY250 pKa = 10.79CLDD253 pKa = 3.75GEE255 pKa = 4.74DD256 pKa = 5.15GVSIWEE262 pKa = 4.36RR263 pKa = 11.84PLGGPGFSVIGVEE276 pKa = 5.14DD277 pKa = 3.79FTGDD281 pKa = 4.31GIPDD285 pKa = 3.67VVAGCSNEE293 pKa = 3.91AEE295 pKa = 4.38TIGYY299 pKa = 10.05AKK301 pKa = 10.26GINGDD306 pKa = 3.04TGAQVWSKK314 pKa = 8.4TASGSSVWALEE325 pKa = 3.87QIEE328 pKa = 5.72DD329 pKa = 3.55ITGDD333 pKa = 3.73GIKK336 pKa = 10.5DD337 pKa = 3.52VIIGDD342 pKa = 3.95FSGNIYY348 pKa = 10.81GLDD351 pKa = 3.48ATDD354 pKa = 4.56GGQEE358 pKa = 3.95YY359 pKa = 8.42STSIGTAIITRR370 pKa = 11.84FAKK373 pKa = 10.82LNDD376 pKa = 3.28VDD378 pKa = 4.73YY379 pKa = 10.74SGHH382 pKa = 6.33PEE384 pKa = 3.61IVPAHH389 pKa = 6.11SSIHH393 pKa = 4.42TTQLIDD399 pKa = 4.25AEE401 pKa = 4.76DD402 pKa = 4.14GSIIWSHH409 pKa = 5.84GVADD413 pKa = 4.11QPWNVARR420 pKa = 11.84ISDD423 pKa = 3.78VSGDD427 pKa = 4.0GIDD430 pKa = 3.98DD431 pKa = 3.87VLVGTLYY438 pKa = 11.0NSNYY442 pKa = 10.12CYY444 pKa = 10.53FLDD447 pKa = 4.03GTNGSEE453 pKa = 5.01LEE455 pKa = 4.32TIAYY459 pKa = 7.64GQAVDD464 pKa = 5.56AINAIPDD471 pKa = 3.61VVADD475 pKa = 4.18GSMEE479 pKa = 3.98MVAGGRR485 pKa = 11.84DD486 pKa = 3.47GKK488 pKa = 10.3IVCFSGGLNAFSPNVEE504 pKa = 3.88VEE506 pKa = 4.03ADD508 pKa = 4.74FKK510 pKa = 11.86ADD512 pKa = 3.08ITEE515 pKa = 4.35GKK517 pKa = 10.62APLTVHH523 pKa = 6.25FTDD526 pKa = 5.58LSIAEE531 pKa = 4.28NTTITSWEE539 pKa = 3.87WDD541 pKa = 3.51FDD543 pKa = 3.76NDD545 pKa = 5.42DD546 pKa = 4.77IIDD549 pKa = 4.05SEE551 pKa = 4.39EE552 pKa = 3.91QNPVWTYY559 pKa = 9.9TEE561 pKa = 4.02GGIYY565 pKa = 9.26TVSLTVSDD573 pKa = 3.59GTIFDD578 pKa = 4.42TEE580 pKa = 4.25TKK582 pKa = 10.23ADD584 pKa = 4.2YY585 pKa = 10.58IAVSQITLEE594 pKa = 4.21IGDD597 pKa = 3.6ITGGLFNVNAVIKK610 pKa = 9.53NTGTIEE616 pKa = 3.73ATGAHH621 pKa = 5.12WRR623 pKa = 11.84ITLEE627 pKa = 3.96GGFILLGRR635 pKa = 11.84NTSGEE640 pKa = 4.16NLSITAGGEE649 pKa = 3.98EE650 pKa = 4.5TVSSKK655 pKa = 11.12LILGFGQTTVTVEE668 pKa = 3.32VWIPDD673 pKa = 3.71GPSDD677 pKa = 3.5TRR679 pKa = 11.84EE680 pKa = 3.52QGGFVIIFFIKK691 pKa = 10.22VNPGGII697 pKa = 3.57

Molecular weight: 74.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M7TDA3|M7TDA3_9ARCH Uncharacterized protein OS=Thermoplasmatales archaeon SCGC AB-540-F20 OX=1242866 GN=MBGDF03_01192 PE=4 SV=1
SS1 pKa = 7.15IYY3 pKa = 10.64NFCFHH8 pKa = 7.01SIIVLSRR15 pKa = 11.84NYY17 pKa = 9.81FLRR20 pKa = 11.84VNKK23 pKa = 8.87VTDD26 pKa = 3.81LVYY29 pKa = 10.13MKK31 pKa = 10.62KK32 pKa = 10.52LNQKK36 pKa = 8.11KK37 pKa = 9.05VRR39 pKa = 11.84WIVRR43 pKa = 11.84EE44 pKa = 3.82MEE46 pKa = 4.1KK47 pKa = 10.72GDD49 pKa = 3.35RR50 pKa = 11.84SVYY53 pKa = 10.33RR54 pKa = 11.84IAKK57 pKa = 8.92TMDD60 pKa = 2.63ITPRR64 pKa = 11.84WVRR67 pKa = 11.84EE68 pKa = 3.71IYY70 pKa = 9.96RR71 pKa = 11.84DD72 pKa = 3.51HH73 pKa = 6.52QRR75 pKa = 11.84TGRR78 pKa = 11.84YY79 pKa = 8.96LYY81 pKa = 7.1PTKK84 pKa = 10.27PGRR87 pKa = 11.84KK88 pKa = 8.04PRR90 pKa = 11.84SISNEE95 pKa = 3.8RR96 pKa = 11.84EE97 pKa = 3.84RR98 pKa = 11.84SCC100 pKa = 5.17

Molecular weight: 12.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1255

0

1255

295448

22

1733

235.4

26.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.334 ± 0.068

1.359 ± 0.038

6.193 ± 0.072

6.733 ± 0.072

4.581 ± 0.055

7.009 ± 0.068

1.842 ± 0.031

9.104 ± 0.094

7.555 ± 0.134

8.35 ± 0.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.391 ± 0.037

5.597 ± 0.089

3.886 ± 0.051

2.447 ± 0.035

3.692 ± 0.061

6.453 ± 0.083

5.702 ± 0.075

6.26 ± 0.054

1.465 ± 0.042

4.047 ± 0.056
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski