Torque teno sus virus 1a
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F8TX29|F8TX29_9VIRU ORF2 OS=Torque teno sus virus 1a OX=687386 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.08 FLYY4 pKa = 9.97 WEE6 pKa = 4.53 EE7 pKa = 3.91 AWLTAIEE14 pKa = 5.38 GYY16 pKa = 10.76 HH17 pKa = 6.65 NLDD20 pKa = 3.54 CRR22 pKa = 11.84 CGNWYY27 pKa = 10.07 DD28 pKa = 3.46 HH29 pKa = 6.1 LQRR32 pKa = 11.84 LCALDD37 pKa = 3.6 QLDD40 pKa = 4.17 AVAAAAIEE48 pKa = 4.35 RR49 pKa = 11.84 EE50 pKa = 4.44 DD51 pKa = 4.2 GDD53 pKa = 4.0 GDD55 pKa = 4.16 AATTDD60 pKa = 4.16 TITDD64 pKa = 3.84 GDD66 pKa = 4.08 PGVAGGG72 pKa = 4.02
Molecular weight: 7.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.771
IPC2_protein 3.783
IPC_protein 3.732
Toseland 3.528
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.706
Rodwell 3.567
Grimsley 3.439
Solomon 3.719
Lehninger 3.681
Nozaki 3.872
DTASelect 4.113
Thurlkill 3.605
EMBOSS 3.719
Sillero 3.859
Patrickios 0.477
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.771
Protein with the highest isoelectric point:
>tr|F8TX29|F8TX29_9VIRU ORF2 OS=Torque teno sus virus 1a OX=687386 GN=ORF2 PE=4 SV=1
MM1 pKa = 8.06 RR2 pKa = 11.84 FRR4 pKa = 11.84 PIRR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 YY14 pKa = 9.34 RR15 pKa = 11.84 KK16 pKa = 9.55 RR17 pKa = 11.84 RR18 pKa = 11.84 WGWRR22 pKa = 11.84 RR23 pKa = 11.84 RR24 pKa = 11.84 YY25 pKa = 9.16 YY26 pKa = 8.7 RR27 pKa = 11.84 HH28 pKa = 6.54 HH29 pKa = 5.95 YY30 pKa = 9.16 RR31 pKa = 11.84 RR32 pKa = 11.84 RR33 pKa = 11.84 PWRR36 pKa = 11.84 RR37 pKa = 11.84 WRR39 pKa = 11.84 VRR41 pKa = 11.84 RR42 pKa = 11.84 WRR44 pKa = 11.84 RR45 pKa = 11.84 SVFRR49 pKa = 11.84 RR50 pKa = 11.84 GGRR53 pKa = 11.84 RR54 pKa = 11.84 ARR56 pKa = 11.84 PYY58 pKa = 10.18 RR59 pKa = 11.84 ISAFNPRR66 pKa = 11.84 VMRR69 pKa = 11.84 RR70 pKa = 11.84 IVIQGWWPLIQCLPGQEE87 pKa = 4.09 HH88 pKa = 6.75 LRR90 pKa = 11.84 YY91 pKa = 9.65 RR92 pKa = 11.84 PLNWDD97 pKa = 3.3 IEE99 pKa = 4.48 KK100 pKa = 10.44 EE101 pKa = 4.22 WVVEE105 pKa = 3.97 KK106 pKa = 10.95 DD107 pKa = 3.44 KK108 pKa = 11.52 EE109 pKa = 4.14 PDD111 pKa = 2.97 KK112 pKa = 10.85 MGYY115 pKa = 9.11 LVQYY119 pKa = 10.1 GGGWGSGHH127 pKa = 6.31 ITLEE131 pKa = 4.07 DD132 pKa = 4.17 LFNEE136 pKa = 4.14 HH137 pKa = 5.92 QKK139 pKa = 8.42 WRR141 pKa = 11.84 NMWSKK146 pKa = 11.53 SNDD149 pKa = 2.85 GMDD152 pKa = 3.68 LVRR155 pKa = 11.84 YY156 pKa = 7.33 FGCKK160 pKa = 8.81 IYY162 pKa = 10.43 LYY164 pKa = 9.82 PLEE167 pKa = 5.55 DD168 pKa = 2.88 VDD170 pKa = 4.93 YY171 pKa = 9.82 WFWWDD176 pKa = 3.06 TDD178 pKa = 3.77 FKK180 pKa = 11.64 NNYY183 pKa = 6.99 EE184 pKa = 4.07 DD185 pKa = 5.33 RR186 pKa = 11.84 IKK188 pKa = 10.44 EE189 pKa = 3.98 YY190 pKa = 10.45 CQPSVMLMAKK200 pKa = 8.32 RR201 pKa = 11.84 TKK203 pKa = 10.03 IVVARR208 pKa = 11.84 KK209 pKa = 8.72 RR210 pKa = 11.84 AQHH213 pKa = 5.56 RR214 pKa = 11.84 RR215 pKa = 11.84 RR216 pKa = 11.84 VKK218 pKa = 10.46 KK219 pKa = 10.42 IFIPPPSRR227 pKa = 11.84 DD228 pKa = 3.04 TTQWKK233 pKa = 9.0 FQTEE237 pKa = 4.5 FCKK240 pKa = 10.7 QPLFTWAAGLIEE252 pKa = 4.38 LQRR255 pKa = 11.84 PFDD258 pKa = 4.05 FNGGFRR264 pKa = 11.84 NAWWLEE270 pKa = 3.49 PRR272 pKa = 11.84 NEE274 pKa = 4.27 AGEE277 pKa = 4.1 MDD279 pKa = 5.56 YY280 pKa = 9.86 ITLWGRR286 pKa = 11.84 VPPQGDD292 pKa = 3.19 TDD294 pKa = 3.81 MPDD297 pKa = 3.28 PNTFKK302 pKa = 8.72 TTTQNKK308 pKa = 9.47 NYY310 pKa = 9.93 SIQPGKK316 pKa = 10.05 EE317 pKa = 3.79 NNVYY321 pKa = 10.44 PIMAYY326 pKa = 9.16 IRR328 pKa = 11.84 QEE330 pKa = 3.79 NQQPVVNWCVCYY342 pKa = 10.89 NKK344 pKa = 10.42 TIDD347 pKa = 3.15 RR348 pKa = 11.84 WRR350 pKa = 11.84 KK351 pKa = 6.27 TQAATLKK358 pKa = 10.18 IGNLRR363 pKa = 11.84 GLCVRR368 pKa = 11.84 EE369 pKa = 4.55 LSRR372 pKa = 11.84 QEE374 pKa = 3.59 MTYY377 pKa = 10.05 KK378 pKa = 10.02 WKK380 pKa = 10.57 QGEE383 pKa = 4.03 FSKK386 pKa = 10.55 AYY388 pKa = 9.41 HH389 pKa = 6.63 DD390 pKa = 3.18 RR391 pKa = 11.84 WGYY394 pKa = 7.7 NTYY397 pKa = 10.33 FVTIDD402 pKa = 3.37 GRR404 pKa = 11.84 KK405 pKa = 7.74 GTEE408 pKa = 3.89 NPTVQIWNFNDD419 pKa = 3.9 KK420 pKa = 9.38 ITVQSGHH427 pKa = 6.48 ILNFAFNIQKK437 pKa = 10.88 SNDD440 pKa = 3.41 NLKK443 pKa = 11.03 DD444 pKa = 3.35 SDD446 pKa = 3.89 FGKK449 pKa = 10.42 IGLPKK454 pKa = 10.24 SPHH457 pKa = 6.57 DD458 pKa = 4.62 LDD460 pKa = 5.33 FGHH463 pKa = 7.24 KK464 pKa = 9.92 NRR466 pKa = 11.84 FGPFVVKK473 pKa = 10.77 NEE475 pKa = 3.89 PVEE478 pKa = 4.23 FQLNPPRR485 pKa = 11.84 AINVWVRR492 pKa = 11.84 YY493 pKa = 10.0 KK494 pKa = 10.85 FFFQFGGEE502 pKa = 4.11 YY503 pKa = 10.14 QPPTGIRR510 pKa = 11.84 DD511 pKa = 3.64 PCLDD515 pKa = 3.5 NPAYY519 pKa = 9.14 PVPQSTGVTHH529 pKa = 7.11 PLFAGAGGITAQVGPSGLTARR550 pKa = 11.84 TIRR553 pKa = 11.84 ALCASPPKK561 pKa = 10.62 AQTSQCAFLGGDD573 pKa = 3.03 RR574 pKa = 11.84 SEE576 pKa = 4.09 EE577 pKa = 4.14 EE578 pKa = 3.98 KK579 pKa = 10.83 RR580 pKa = 11.84 KK581 pKa = 8.29 EE582 pKa = 4.12 TQEE585 pKa = 4.05 TSSEE589 pKa = 4.11 SSITSAEE596 pKa = 4.07 SSTEE600 pKa = 3.94 GDD602 pKa = 3.09 GSTDD606 pKa = 3.12 EE607 pKa = 4.12 EE608 pKa = 4.11 EE609 pKa = 3.9 RR610 pKa = 11.84 RR611 pKa = 11.84 EE612 pKa = 3.76 RR613 pKa = 11.84 RR614 pKa = 11.84 RR615 pKa = 11.84 RR616 pKa = 11.84 RR617 pKa = 11.84 RR618 pKa = 11.84 KK619 pKa = 8.84 RR620 pKa = 11.84 QLKK623 pKa = 10.15 LFYY626 pKa = 10.37 QRR628 pKa = 11.84 LTDD631 pKa = 4.24 RR632 pKa = 11.84 YY633 pKa = 9.55 LEE635 pKa = 4.11 HH636 pKa = 6.71 KK637 pKa = 10.16 RR638 pKa = 11.84 RR639 pKa = 11.84 RR640 pKa = 11.84 LSEE643 pKa = 3.83
Molecular weight: 77.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.299
IPC2_protein 9.531
IPC_protein 9.984
Toseland 10.277
ProMoST 9.984
Dawson 10.452
Bjellqvist 10.16
Wikipedia 10.643
Rodwell 10.745
Grimsley 10.526
Solomon 10.496
Lehninger 10.452
Nozaki 10.277
DTASelect 10.145
Thurlkill 10.321
EMBOSS 10.687
Sillero 10.379
Patrickios 10.306
IPC_peptide 10.496
IPC2_peptide 9.033
IPC2.peptide.svr19 8.607
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
715
72
643
357.5
42.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.035 ± 4.19
1.818 ± 0.961
5.874 ± 3.279
6.014 ± 0.381
4.615 ± 1.32
7.273 ± 1.57
1.958 ± 0.335
4.615 ± 0.184
5.734 ± 2.346
5.874 ± 1.574
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.678 ± 0.118
4.755 ± 0.809
5.734 ± 1.778
4.895 ± 0.866
11.748 ± 3.102
3.916 ± 1.602
5.594 ± 0.552
4.476 ± 0.695
4.056 ± 0.045
4.336 ± 0.069
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here