Opitutus sp. GAS368

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Opitutae; Opitutales; Opitutaceae; Opitutus; unclassified Opitutus

Average proteome isoelectric point is 7.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3522 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H1TGM8|A0A1H1TGM8_9BACT 3-methyladenine DNA glycosylase AlkD OS=Opitutus sp. GAS368 OX=1882749 GN=SAMN05444173_3361 PE=4 SV=1
MM1 pKa = 7.71HH2 pKa = 8.08DD3 pKa = 4.11SLNIEE8 pKa = 4.65CPHH11 pKa = 7.37CGEE14 pKa = 4.39TFSLALDD21 pKa = 3.72TSEE24 pKa = 4.44GSAEE28 pKa = 3.92FTVDD32 pKa = 4.66CEE34 pKa = 4.7VCCRR38 pKa = 11.84PMTVSVRR45 pKa = 11.84ISDD48 pKa = 3.88DD49 pKa = 3.87GEE51 pKa = 4.3LEE53 pKa = 4.22GVDD56 pKa = 3.62VTEE59 pKa = 4.42EE60 pKa = 3.81

Molecular weight: 6.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H1NIL1|A0A1H1NIL1_9BACT Putative glutamate--cysteine ligase 2 OS=Opitutus sp. GAS368 OX=1882749 GN=SAMN05444173_1544 PE=3 SV=1
MM1 pKa = 7.52KK2 pKa = 8.83PTFRR6 pKa = 11.84PHH8 pKa = 5.67RR9 pKa = 11.84KK10 pKa = 8.93KK11 pKa = 10.33RR12 pKa = 11.84ARR14 pKa = 11.84KK15 pKa = 8.4IGFRR19 pKa = 11.84ARR21 pKa = 11.84KK22 pKa = 6.85ATRR25 pKa = 11.84GGRR28 pKa = 11.84KK29 pKa = 8.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.87GRR39 pKa = 11.84KK40 pKa = 8.92RR41 pKa = 11.84LTVVV45 pKa = 3.08

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3522

0

3522

1231825

29

8496

349.8

38.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.205 ± 0.055

0.899 ± 0.014

4.964 ± 0.036

5.188 ± 0.053

4.044 ± 0.029

8.297 ± 0.082

2.239 ± 0.025

4.544 ± 0.034

3.889 ± 0.049

10.796 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.915 ± 0.018

2.94 ± 0.047

5.649 ± 0.042

3.384 ± 0.027

6.787 ± 0.06

5.077 ± 0.042

5.821 ± 0.1

7.278 ± 0.035

1.557 ± 0.024

2.527 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski