Mycobacterium phage Theia

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 5.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N9SKX2|A0A0N9SKX2_9CAUD Major tail protein OS=Mycobacterium phage Theia OX=1718172 GN=17 PE=4 SV=1
MM1 pKa = 7.16QMAKK5 pKa = 9.85IYY7 pKa = 9.54VTDD10 pKa = 4.24LAAYY14 pKa = 10.38NNGDD18 pKa = 4.94LVGKK22 pKa = 9.34WIDD25 pKa = 3.2LHH27 pKa = 6.1QFSDD31 pKa = 4.0VEE33 pKa = 4.23DD34 pKa = 3.92LKK36 pKa = 11.51SEE38 pKa = 4.07IKK40 pKa = 9.1THH42 pKa = 5.32VLLDD46 pKa = 3.71GHH48 pKa = 6.12EE49 pKa = 3.98EE50 pKa = 4.0WAIHH54 pKa = 4.98DD55 pKa = 3.87HH56 pKa = 6.5EE57 pKa = 5.23GFGEE61 pKa = 4.06LRR63 pKa = 11.84ISEE66 pKa = 4.44YY67 pKa = 11.08EE68 pKa = 4.13DD69 pKa = 4.35LDD71 pKa = 3.83VMLEE75 pKa = 3.88LSEE78 pKa = 5.07AVEE81 pKa = 3.97KK82 pKa = 11.14HH83 pKa = 5.72GDD85 pKa = 3.7PFVAWIGDD93 pKa = 3.54IVGDD97 pKa = 3.4IGYY100 pKa = 9.64FGSVSEE106 pKa = 4.83AVAQFEE112 pKa = 4.32DD113 pKa = 5.01VYY115 pKa = 10.22IGPMTIEE122 pKa = 4.62DD123 pKa = 3.76YY124 pKa = 10.57AYY126 pKa = 9.76EE127 pKa = 4.08YY128 pKa = 11.32AEE130 pKa = 4.25EE131 pKa = 4.13VLGLRR136 pKa = 11.84GTALEE141 pKa = 4.23YY142 pKa = 10.74FDD144 pKa = 4.16TEE146 pKa = 3.93KK147 pKa = 10.56FARR150 pKa = 11.84DD151 pKa = 3.51LEE153 pKa = 4.42ASGDD157 pKa = 3.87VSEE160 pKa = 4.43AWYY163 pKa = 10.33DD164 pKa = 3.22GEE166 pKa = 4.72YY167 pKa = 10.77YY168 pKa = 10.83VFRR171 pKa = 11.84SWW173 pKa = 3.74

Molecular weight: 19.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N9RS49|A0A0N9RS49_9CAUD Lysin B OS=Mycobacterium phage Theia OX=1718172 GN=7 PE=4 SV=1
MM1 pKa = 7.49NKK3 pKa = 8.0QQRR6 pKa = 11.84VYY8 pKa = 11.11LDD10 pKa = 3.04GKK12 pKa = 10.56VYY14 pKa = 10.09VARR17 pKa = 11.84GNHH20 pKa = 4.97IVRR23 pKa = 11.84IRR25 pKa = 3.18

Molecular weight: 3.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

87

0

87

15963

25

1112

183.5

20.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.284 ± 0.359

0.864 ± 0.132

7.004 ± 0.212

7.467 ± 0.294

3.383 ± 0.2

8.526 ± 0.483

1.936 ± 0.15

4.993 ± 0.167

4.636 ± 0.294

8.232 ± 0.296

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.337 ± 0.113

3.326 ± 0.155

5.03 ± 0.258

3.345 ± 0.249

5.932 ± 0.304

5.419 ± 0.187

5.851 ± 0.274

7.448 ± 0.272

1.948 ± 0.151

3.038 ± 0.247
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski