Acidianus two-tailed virus 2

Taxonomy: Viruses; Bicaudaviridae; Bicaudavirus; unclassified Bicaudavirus

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C9EG19|A0A1C9EG19_9VIRU Uncharacterized protein OS=Acidianus two-tailed virus 2 OX=1898549 PE=4 SV=1
MM1 pKa = 7.45GLAPSTTTLAIALVFLGISLIFLVPVMVQGIFSIVSGVFTVVSNEE46 pKa = 4.13SNTTGYY52 pKa = 8.76PQSTEE57 pKa = 4.0VVQLSNQTASVSVPSQYY74 pKa = 11.61ANTYY78 pKa = 9.28NLYY81 pKa = 10.72LSFISFVGSIFTDD94 pKa = 5.11PIALIMLILVSLIIVLFAFYY114 pKa = 10.8YY115 pKa = 10.43KK116 pKa = 10.72NQGG119 pKa = 3.05

Molecular weight: 12.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C9EG49|A0A1C9EG49_9VIRU Radical SAM protein OS=Acidianus two-tailed virus 2 OX=1898549 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 10.47VKK4 pKa = 10.3LVKK7 pKa = 10.7AEE9 pKa = 3.8ARR11 pKa = 11.84TNSSFYY17 pKa = 9.68YY18 pKa = 10.47LVFISMMLILLRR30 pKa = 11.84YY31 pKa = 8.35IVNIRR36 pKa = 11.84SLSFRR41 pKa = 11.84RR42 pKa = 11.84VKK44 pKa = 10.53VSKK47 pKa = 10.8KK48 pKa = 9.88NLLVRR53 pKa = 11.84AGGFDD58 pKa = 3.71SKK60 pKa = 11.43SLEE63 pKa = 4.08NLSMGIIFIIVLLAVGGFIVADD85 pKa = 3.88LFVTLGSYY93 pKa = 8.76TPSVSSSNPLYY104 pKa = 10.63NATTAVVHH112 pKa = 6.29GMQALGGLFSSSIGLITILIIIAIVSVVIALLRR145 pKa = 11.84SSFSSGGTGTT155 pKa = 3.75

Molecular weight: 16.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

17675

37

1944

280.6

31.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.969 ± 0.294

0.475 ± 0.085

4.611 ± 0.612

7.004 ± 0.757

4.588 ± 0.354

5.516 ± 0.504

1.103 ± 0.129

7.615 ± 0.274

7.83 ± 0.737

9.714 ± 0.441

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.184 ± 0.165

5.437 ± 0.299

4.775 ± 0.275

4.102 ± 0.335

3.479 ± 0.337

6.936 ± 0.56

6.184 ± 0.471

7.021 ± 0.353

0.792 ± 0.148

4.662 ± 0.444
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski