Amino acid dipepetide frequency for Pontimonas phage phiPsal1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.778AlaAla: 7.778 ± 1.004
0.767AlaCys: 0.767 ± 0.311
4.711AlaAsp: 4.711 ± 0.791
6.573AlaGlu: 6.573 ± 0.999
4.053AlaPhe: 4.053 ± 0.68
8.326AlaGly: 8.326 ± 0.937
1.205AlaHis: 1.205 ± 0.442
5.368AlaIle: 5.368 ± 0.749
3.506AlaLys: 3.506 ± 0.702
8.983AlaLeu: 8.983 ± 0.919
2.41AlaMet: 2.41 ± 0.494
3.506AlaAsn: 3.506 ± 0.752
3.287AlaPro: 3.287 ± 0.637
4.053AlaGln: 4.053 ± 0.512
7.34AlaArg: 7.34 ± 1.022
6.025AlaSer: 6.025 ± 0.608
6.025AlaThr: 6.025 ± 0.839
9.641AlaVal: 9.641 ± 1.227
0.986AlaTrp: 0.986 ± 0.292
1.643AlaTyr: 1.643 ± 0.481
0.0AlaXaa: 0.0 ± 0.0
Cys
0.329CysAla: 0.329 ± 0.173
0.0CysCys: 0.0 ± 0.0
0.11CysAsp: 0.11 ± 0.113
0.767CysGlu: 0.767 ± 0.309
0.329CysPhe: 0.329 ± 0.186
0.329CysGly: 0.329 ± 0.198
0.11CysHis: 0.11 ± 0.104
0.438CysIle: 0.438 ± 0.235
0.11CysLys: 0.11 ± 0.093
0.657CysLeu: 0.657 ± 0.263
0.329CysMet: 0.329 ± 0.233
0.11CysAsn: 0.11 ± 0.104
0.438CysPro: 0.438 ± 0.206
0.548CysGln: 0.548 ± 0.27
0.219CysArg: 0.219 ± 0.134
0.438CysSer: 0.438 ± 0.204
0.219CysThr: 0.219 ± 0.142
0.329CysVal: 0.329 ± 0.206
0.11CysTrp: 0.11 ± 0.093
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
4.382AspAla: 4.382 ± 0.763
0.219AspCys: 0.219 ± 0.144
4.711AspAsp: 4.711 ± 0.636
4.82AspGlu: 4.82 ± 0.681
2.41AspPhe: 2.41 ± 0.505
6.135AspGly: 6.135 ± 0.652
0.657AspHis: 0.657 ± 0.229
3.834AspIle: 3.834 ± 0.544
2.082AspLys: 2.082 ± 0.41
7.011AspLeu: 7.011 ± 0.889
0.876AspMet: 0.876 ± 0.292
3.396AspAsn: 3.396 ± 0.545
2.848AspPro: 2.848 ± 0.571
2.41AspGln: 2.41 ± 0.546
3.506AspArg: 3.506 ± 0.618
3.067AspSer: 3.067 ± 0.459
1.972AspThr: 1.972 ± 0.378
5.587AspVal: 5.587 ± 0.76
1.534AspTrp: 1.534 ± 0.41
1.862AspTyr: 1.862 ± 0.468
0.0AspXaa: 0.0 ± 0.0
Glu
8.326GluAla: 8.326 ± 1.294
0.329GluCys: 0.329 ± 0.157
4.382GluAsp: 4.382 ± 0.59
5.149GluGlu: 5.149 ± 1.133
2.629GluPhe: 2.629 ± 0.491
4.492GluGly: 4.492 ± 0.927
1.424GluHis: 1.424 ± 0.457
2.739GluIle: 2.739 ± 0.52
2.958GluLys: 2.958 ± 0.531
5.916GluLeu: 5.916 ± 0.887
1.753GluMet: 1.753 ± 0.495
2.848GluAsn: 2.848 ± 0.588
3.287GluPro: 3.287 ± 0.532
2.52GluGln: 2.52 ± 0.524
4.382GluArg: 4.382 ± 0.55
3.177GluSer: 3.177 ± 0.649
5.149GluThr: 5.149 ± 0.773
5.368GluVal: 5.368 ± 0.75
1.315GluTrp: 1.315 ± 0.403
1.972GluTyr: 1.972 ± 0.521
0.0GluXaa: 0.0 ± 0.0
Phe
3.287PheAla: 3.287 ± 0.799
0.329PheCys: 0.329 ± 0.189
4.601PheAsp: 4.601 ± 0.702
3.396PheGlu: 3.396 ± 0.614
1.424PhePhe: 1.424 ± 0.342
5.039PheGly: 5.039 ± 0.705
1.096PheHis: 1.096 ± 0.386
2.52PheIle: 2.52 ± 0.485
0.767PheLys: 0.767 ± 0.267
2.629PheLeu: 2.629 ± 0.564
1.315PheMet: 1.315 ± 0.355
1.643PheAsn: 1.643 ± 0.363
1.205PhePro: 1.205 ± 0.282
0.986PheGln: 0.986 ± 0.341
1.972PheArg: 1.972 ± 0.4
2.958PheSer: 2.958 ± 0.61
2.958PheThr: 2.958 ± 0.571
2.082PheVal: 2.082 ± 0.492
0.548PheTrp: 0.548 ± 0.283
0.986PheTyr: 0.986 ± 0.334
0.0PheXaa: 0.0 ± 0.0
Gly
5.916GlyAla: 5.916 ± 1.024
0.329GlyCys: 0.329 ± 0.215
4.82GlyAsp: 4.82 ± 0.696
6.573GlyGlu: 6.573 ± 0.838
5.478GlyPhe: 5.478 ± 0.807
6.464GlyGly: 6.464 ± 0.887
2.301GlyHis: 2.301 ± 0.529
3.396GlyIle: 3.396 ± 0.823
3.725GlyLys: 3.725 ± 0.628
7.669GlyLeu: 7.669 ± 1.012
1.862GlyMet: 1.862 ± 0.55
2.958GlyAsn: 2.958 ± 0.567
1.862GlyPro: 1.862 ± 0.386
2.629GlyGln: 2.629 ± 0.622
4.601GlyArg: 4.601 ± 0.56
5.806GlySer: 5.806 ± 0.796
4.711GlyThr: 4.711 ± 0.742
7.121GlyVal: 7.121 ± 0.712
1.643GlyTrp: 1.643 ± 0.445
2.301GlyTyr: 2.301 ± 0.711
0.0GlyXaa: 0.0 ± 0.0
His
1.315HisAla: 1.315 ± 0.509
0.11HisCys: 0.11 ± 0.11
1.753HisAsp: 1.753 ± 0.49
1.315HisGlu: 1.315 ± 0.444
0.548HisPhe: 0.548 ± 0.25
1.315HisGly: 1.315 ± 0.455
0.438HisHis: 0.438 ± 0.242
0.876HisIle: 0.876 ± 0.304
1.096HisLys: 1.096 ± 0.421
2.191HisLeu: 2.191 ± 0.413
0.329HisMet: 0.329 ± 0.175
0.876HisAsn: 0.876 ± 0.264
0.986HisPro: 0.986 ± 0.29
0.219HisGln: 0.219 ± 0.148
1.205HisArg: 1.205 ± 0.457
0.657HisSer: 0.657 ± 0.277
1.534HisThr: 1.534 ± 0.428
1.643HisVal: 1.643 ± 0.379
0.11HisTrp: 0.11 ± 0.095
0.438HisTyr: 0.438 ± 0.202
0.0HisXaa: 0.0 ± 0.0
Ile
5.697IleAla: 5.697 ± 0.733
0.438IleCys: 0.438 ± 0.262
3.615IleAsp: 3.615 ± 0.762
5.039IleGlu: 5.039 ± 0.802
1.643IlePhe: 1.643 ± 0.444
3.944IleGly: 3.944 ± 0.875
0.876IleHis: 0.876 ± 0.335
2.082IleIle: 2.082 ± 0.577
2.301IleLys: 2.301 ± 0.444
2.082IleLeu: 2.082 ± 0.429
0.438IleMet: 0.438 ± 0.234
1.424IleAsn: 1.424 ± 0.356
3.067IlePro: 3.067 ± 0.688
1.972IleGln: 1.972 ± 0.426
2.41IleArg: 2.41 ± 0.461
2.301IleSer: 2.301 ± 0.484
2.739IleThr: 2.739 ± 0.529
4.053IleVal: 4.053 ± 0.664
0.329IleTrp: 0.329 ± 0.193
0.986IleTyr: 0.986 ± 0.374
0.0IleXaa: 0.0 ± 0.0
Lys
4.273LysAla: 4.273 ± 0.565
0.11LysCys: 0.11 ± 0.127
2.191LysAsp: 2.191 ± 0.746
1.096LysGlu: 1.096 ± 0.356
1.534LysPhe: 1.534 ± 0.489
3.396LysGly: 3.396 ± 0.775
0.767LysHis: 0.767 ± 0.347
2.301LysIle: 2.301 ± 0.503
2.191LysLys: 2.191 ± 0.47
4.163LysLeu: 4.163 ± 0.588
1.972LysMet: 1.972 ± 0.443
1.096LysAsn: 1.096 ± 0.328
2.629LysPro: 2.629 ± 0.707
1.972LysGln: 1.972 ± 0.435
2.739LysArg: 2.739 ± 0.546
2.41LysSer: 2.41 ± 0.51
2.082LysThr: 2.082 ± 0.509
3.396LysVal: 3.396 ± 0.585
1.096LysTrp: 1.096 ± 0.408
0.438LysTyr: 0.438 ± 0.271
0.0LysXaa: 0.0 ± 0.0
Leu
8.874LeuAla: 8.874 ± 1.003
0.657LeuCys: 0.657 ± 0.3
6.354LeuAsp: 6.354 ± 1.069
7.997LeuGlu: 7.997 ± 0.898
2.41LeuPhe: 2.41 ± 0.442
7.669LeuGly: 7.669 ± 0.918
1.096LeuHis: 1.096 ± 0.312
3.725LeuIle: 3.725 ± 0.658
2.958LeuLys: 2.958 ± 0.588
6.683LeuLeu: 6.683 ± 1.001
3.177LeuMet: 3.177 ± 0.747
2.848LeuAsn: 2.848 ± 0.396
4.382LeuPro: 4.382 ± 0.704
2.41LeuGln: 2.41 ± 0.559
5.478LeuArg: 5.478 ± 0.543
3.506LeuSer: 3.506 ± 0.579
5.697LeuThr: 5.697 ± 0.7
7.011LeuVal: 7.011 ± 1.241
1.643LeuTrp: 1.643 ± 0.397
1.862LeuTyr: 1.862 ± 0.599
0.0LeuXaa: 0.0 ± 0.0
Met
3.615MetAla: 3.615 ± 0.564
0.219MetCys: 0.219 ± 0.173
1.643MetAsp: 1.643 ± 0.358
1.424MetGlu: 1.424 ± 0.373
1.534MetPhe: 1.534 ± 0.324
2.191MetGly: 2.191 ± 0.405
0.219MetHis: 0.219 ± 0.152
1.315MetIle: 1.315 ± 0.418
1.096MetLys: 1.096 ± 0.464
1.534MetLeu: 1.534 ± 0.444
0.329MetMet: 0.329 ± 0.16
0.986MetAsn: 0.986 ± 0.339
1.643MetPro: 1.643 ± 0.469
0.548MetGln: 0.548 ± 0.237
1.205MetArg: 1.205 ± 0.401
1.972MetSer: 1.972 ± 0.404
1.534MetThr: 1.534 ± 0.383
1.205MetVal: 1.205 ± 0.431
0.329MetTrp: 0.329 ± 0.191
0.0MetTyr: 0.0 ± 0.0
0.0MetXaa: 0.0 ± 0.0
Asn
3.725AsnAla: 3.725 ± 0.559
0.11AsnCys: 0.11 ± 0.104
1.534AsnAsp: 1.534 ± 0.497
2.848AsnGlu: 2.848 ± 0.493
1.424AsnPhe: 1.424 ± 0.32
3.177AsnGly: 3.177 ± 0.414
1.205AsnHis: 1.205 ± 0.292
1.643AsnIle: 1.643 ± 0.39
1.753AsnLys: 1.753 ± 0.434
3.506AsnLeu: 3.506 ± 0.714
0.876AsnMet: 0.876 ± 0.309
0.548AsnAsn: 0.548 ± 0.203
2.41AsnPro: 2.41 ± 0.435
1.424AsnGln: 1.424 ± 0.459
2.41AsnArg: 2.41 ± 0.52
2.301AsnSer: 2.301 ± 0.372
1.972AsnThr: 1.972 ± 0.391
2.629AsnVal: 2.629 ± 0.493
0.438AsnTrp: 0.438 ± 0.211
0.876AsnTyr: 0.876 ± 0.285
0.0AsnXaa: 0.0 ± 0.0
Pro
4.273ProAla: 4.273 ± 0.826
0.219ProCys: 0.219 ± 0.142
3.944ProAsp: 3.944 ± 0.762
3.287ProGlu: 3.287 ± 0.684
1.424ProPhe: 1.424 ± 0.345
3.506ProGly: 3.506 ± 0.578
1.753ProHis: 1.753 ± 0.709
2.52ProIle: 2.52 ± 0.459
2.629ProLys: 2.629 ± 0.559
2.629ProLeu: 2.629 ± 0.702
0.548ProMet: 0.548 ± 0.254
2.082ProAsn: 2.082 ± 0.657
1.862ProPro: 1.862 ± 0.354
1.643ProGln: 1.643 ± 0.35
2.41ProArg: 2.41 ± 0.57
2.848ProSer: 2.848 ± 0.478
3.067ProThr: 3.067 ± 0.579
4.163ProVal: 4.163 ± 0.741
0.438ProTrp: 0.438 ± 0.202
0.767ProTyr: 0.767 ± 0.253
0.0ProXaa: 0.0 ± 0.0
Gln
3.506GlnAla: 3.506 ± 0.605
0.11GlnCys: 0.11 ± 0.101
1.753GlnAsp: 1.753 ± 0.385
1.753GlnGlu: 1.753 ± 0.44
1.315GlnPhe: 1.315 ± 0.391
2.191GlnGly: 2.191 ± 0.552
0.876GlnHis: 0.876 ± 0.296
1.534GlnIle: 1.534 ± 0.508
1.534GlnLys: 1.534 ± 0.365
3.287GlnLeu: 3.287 ± 0.478
0.876GlnMet: 0.876 ± 0.246
0.986GlnAsn: 0.986 ± 0.359
1.315GlnPro: 1.315 ± 0.425
1.862GlnGln: 1.862 ± 0.612
2.082GlnArg: 2.082 ± 0.511
1.862GlnSer: 1.862 ± 0.355
2.082GlnThr: 2.082 ± 0.489
3.834GlnVal: 3.834 ± 0.773
0.986GlnTrp: 0.986 ± 0.328
1.096GlnTyr: 1.096 ± 0.29
0.0GlnXaa: 0.0 ± 0.0
Arg
5.587ArgAla: 5.587 ± 0.925
0.329ArgCys: 0.329 ± 0.202
3.834ArgAsp: 3.834 ± 0.594
3.396ArgGlu: 3.396 ± 0.526
2.739ArgPhe: 2.739 ± 0.533
4.382ArgGly: 4.382 ± 0.636
1.315ArgHis: 1.315 ± 0.395
2.958ArgIle: 2.958 ± 0.507
2.191ArgLys: 2.191 ± 0.538
5.368ArgLeu: 5.368 ± 0.752
2.301ArgMet: 2.301 ± 0.355
3.177ArgAsn: 3.177 ± 0.768
2.301ArgPro: 2.301 ± 0.672
1.862ArgGln: 1.862 ± 0.454
5.259ArgArg: 5.259 ± 0.824
2.848ArgSer: 2.848 ± 0.467
3.396ArgThr: 3.396 ± 0.743
5.368ArgVal: 5.368 ± 0.866
1.643ArgTrp: 1.643 ± 0.397
1.205ArgTyr: 1.205 ± 0.293
0.0ArgXaa: 0.0 ± 0.0
Ser
5.259SerAla: 5.259 ± 0.867
0.219SerCys: 0.219 ± 0.139
2.52SerAsp: 2.52 ± 0.356
4.492SerGlu: 4.492 ± 0.796
2.848SerPhe: 2.848 ± 0.489
4.492SerGly: 4.492 ± 0.842
0.876SerHis: 0.876 ± 0.334
2.629SerIle: 2.629 ± 0.55
2.41SerLys: 2.41 ± 0.475
4.93SerLeu: 4.93 ± 0.654
1.862SerMet: 1.862 ± 0.327
2.301SerAsn: 2.301 ± 0.628
2.739SerPro: 2.739 ± 0.536
2.301SerGln: 2.301 ± 0.439
3.287SerArg: 3.287 ± 0.607
3.506SerSer: 3.506 ± 0.594
4.382SerThr: 4.382 ± 0.684
3.944SerVal: 3.944 ± 0.596
1.315SerTrp: 1.315 ± 0.465
1.315SerTyr: 1.315 ± 0.325
0.0SerXaa: 0.0 ± 0.0
Thr
7.669ThrAla: 7.669 ± 0.872
0.767ThrCys: 0.767 ± 0.281
3.177ThrAsp: 3.177 ± 0.592
3.177ThrGlu: 3.177 ± 0.551
2.52ThrPhe: 2.52 ± 0.644
4.93ThrGly: 4.93 ± 0.695
0.876ThrHis: 0.876 ± 0.312
2.191ThrIle: 2.191 ± 0.482
2.41ThrLys: 2.41 ± 0.727
5.916ThrLeu: 5.916 ± 0.869
0.876ThrMet: 0.876 ± 0.369
1.643ThrAsn: 1.643 ± 0.528
3.177ThrPro: 3.177 ± 0.666
1.096ThrGln: 1.096 ± 0.307
3.287ThrArg: 3.287 ± 0.575
3.834ThrSer: 3.834 ± 0.641
2.958ThrThr: 2.958 ± 0.755
6.683ThrVal: 6.683 ± 0.935
1.534ThrTrp: 1.534 ± 0.34
1.424ThrTyr: 1.424 ± 0.364
0.0ThrXaa: 0.0 ± 0.0
Val
8.874ValAla: 8.874 ± 1.09
0.438ValCys: 0.438 ± 0.21
5.039ValAsp: 5.039 ± 0.762
4.93ValGlu: 4.93 ± 0.683
4.053ValPhe: 4.053 ± 0.797
5.806ValGly: 5.806 ± 0.665
1.096ValHis: 1.096 ± 0.348
3.944ValIle: 3.944 ± 0.619
4.163ValLys: 4.163 ± 0.803
7.559ValLeu: 7.559 ± 1.097
1.424ValMet: 1.424 ± 0.427
2.739ValAsn: 2.739 ± 0.556
4.382ValPro: 4.382 ± 0.758
2.191ValGln: 2.191 ± 0.613
5.368ValArg: 5.368 ± 0.619
5.259ValSer: 5.259 ± 0.864
5.478ValThr: 5.478 ± 1.099
8.107ValVal: 8.107 ± 1.887
1.315ValTrp: 1.315 ± 0.345
2.191ValTyr: 2.191 ± 0.459
0.0ValXaa: 0.0 ± 0.0
Trp
1.643TrpAla: 1.643 ± 0.477
0.0TrpCys: 0.0 ± 0.0
1.096TrpAsp: 1.096 ± 0.393
0.986TrpGlu: 0.986 ± 0.444
0.657TrpPhe: 0.657 ± 0.298
1.972TrpGly: 1.972 ± 0.438
0.657TrpHis: 0.657 ± 0.254
0.767TrpIle: 0.767 ± 0.253
0.986TrpLys: 0.986 ± 0.226
1.972TrpLeu: 1.972 ± 0.533
0.438TrpMet: 0.438 ± 0.217
0.438TrpAsn: 0.438 ± 0.245
0.657TrpPro: 0.657 ± 0.218
0.986TrpGln: 0.986 ± 0.316
0.767TrpArg: 0.767 ± 0.321
1.096TrpSer: 1.096 ± 0.517
0.986TrpThr: 0.986 ± 0.325
1.315TrpVal: 1.315 ± 0.385
0.767TrpTrp: 0.767 ± 0.37
0.219TrpTyr: 0.219 ± 0.151
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.862TyrAla: 1.862 ± 0.431
0.219TyrCys: 0.219 ± 0.141
1.534TyrAsp: 1.534 ± 0.321
1.096TyrGlu: 1.096 ± 0.314
0.767TyrPhe: 0.767 ± 0.331
2.41TyrGly: 2.41 ± 0.452
0.11TyrHis: 0.11 ± 0.102
0.657TyrIle: 0.657 ± 0.256
0.986TyrLys: 0.986 ± 0.359
1.753TyrLeu: 1.753 ± 0.413
0.438TyrMet: 0.438 ± 0.216
1.205TyrAsn: 1.205 ± 0.38
1.534TyrPro: 1.534 ± 0.48
1.205TyrGln: 1.205 ± 0.326
1.534TyrArg: 1.534 ± 0.418
1.753TyrSer: 1.753 ± 0.386
1.205TyrThr: 1.205 ± 0.329
0.876TyrVal: 0.876 ± 0.315
0.329TyrTrp: 0.329 ± 0.199
0.219TyrTyr: 0.219 ± 0.144
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (9129 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski