Influenza A virus (A/swine/Korea/S452/2004(H9N2))

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus; Influenza A virus; H9N2 subtype

Average proteome isoelectric point is 7.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5Q156|Q5Q156_9INFA Non-structural protein 1 OS=Influenza A virus (A/swine/Korea/S452/2004(H9N2)) OX=300749 GN=NS PE=3 SV=1
MM1 pKa = 7.82EE2 pKa = 5.88DD3 pKa = 4.23FVRR6 pKa = 11.84QCFNPMIVEE15 pKa = 4.33LAEE18 pKa = 4.23KK19 pKa = 10.56AMKK22 pKa = 10.25EE23 pKa = 3.99YY24 pKa = 11.35GEE26 pKa = 4.4DD27 pKa = 3.65PKK29 pKa = 10.9IEE31 pKa = 4.27TNKK34 pKa = 9.68FAAICSHH41 pKa = 6.98LEE43 pKa = 3.77VCFMYY48 pKa = 10.77SDD50 pKa = 3.5FHH52 pKa = 8.77FIDD55 pKa = 3.6EE56 pKa = 4.51RR57 pKa = 11.84GEE59 pKa = 4.25SIIVEE64 pKa = 4.23SGDD67 pKa = 3.45PNALLKK73 pKa = 10.77HH74 pKa = 5.93RR75 pKa = 11.84FEE77 pKa = 6.44IIEE80 pKa = 4.15GRR82 pKa = 11.84DD83 pKa = 2.82RR84 pKa = 11.84TMAWTVVNSICNTTGVEE101 pKa = 4.21KK102 pKa = 10.67PKK104 pKa = 10.37FLPGLYY110 pKa = 9.71DD111 pKa = 3.41YY112 pKa = 11.32KK113 pKa = 10.79EE114 pKa = 3.77NRR116 pKa = 11.84FIEE119 pKa = 4.17IGVTRR124 pKa = 11.84RR125 pKa = 11.84EE126 pKa = 3.6VHH128 pKa = 6.1IYY130 pKa = 10.32YY131 pKa = 10.35LEE133 pKa = 4.16KK134 pKa = 10.68ANKK137 pKa = 8.54IKK139 pKa = 10.7SEE141 pKa = 3.97KK142 pKa = 7.72THH144 pKa = 6.1IHH146 pKa = 5.76IFSFTGEE153 pKa = 3.81EE154 pKa = 4.08MATKK158 pKa = 10.24SDD160 pKa = 3.61YY161 pKa = 11.14TLDD164 pKa = 3.49EE165 pKa = 4.2EE166 pKa = 4.91SRR168 pKa = 11.84ARR170 pKa = 11.84TKK172 pKa = 10.34TRR174 pKa = 11.84LFTIRR179 pKa = 11.84QEE181 pKa = 3.98MAGRR185 pKa = 11.84GLWDD189 pKa = 3.4SFRR192 pKa = 11.84QSEE195 pKa = 4.27RR196 pKa = 11.84GEE198 pKa = 3.89EE199 pKa = 4.16TIEE202 pKa = 3.68EE203 pKa = 4.17RR204 pKa = 11.84FEE206 pKa = 3.72ITGTMRR212 pKa = 11.84RR213 pKa = 11.84LADD216 pKa = 3.3QSLPPNFSSLEE227 pKa = 3.83NFRR230 pKa = 11.84AYY232 pKa = 11.05VDD234 pKa = 3.5GFEE237 pKa = 4.68PNGCIEE243 pKa = 4.47GKK245 pKa = 9.96LSQMSKK251 pKa = 9.86EE252 pKa = 3.84VNARR256 pKa = 11.84IEE258 pKa = 4.18PFLRR262 pKa = 11.84TTPRR266 pKa = 11.84PLRR269 pKa = 11.84LPDD272 pKa = 4.62GPPCSQRR279 pKa = 11.84SKK281 pKa = 11.21FLLLDD286 pKa = 3.78ALKK289 pKa = 10.88LSIEE293 pKa = 4.56DD294 pKa = 4.07PSHH297 pKa = 6.12EE298 pKa = 4.34GEE300 pKa = 5.39GIPLYY305 pKa = 10.72DD306 pKa = 5.54AIKK309 pKa = 10.45CMKK312 pKa = 9.82TFFGWKK318 pKa = 8.64EE319 pKa = 3.72PNIVKK324 pKa = 9.51PHH326 pKa = 6.17EE327 pKa = 4.45KK328 pKa = 10.38GINPNYY334 pKa = 9.91LLAWKK339 pKa = 9.87QVLAEE344 pKa = 4.25LQDD347 pKa = 3.52IEE349 pKa = 4.52NEE351 pKa = 4.07EE352 pKa = 4.77KK353 pKa = 10.44IPKK356 pKa = 7.57TKK358 pKa = 10.66NMKK361 pKa = 8.76KK362 pKa = 8.75TSQLRR367 pKa = 11.84WALGEE372 pKa = 3.86NMAPEE377 pKa = 4.21KK378 pKa = 10.99VDD380 pKa = 5.73FEE382 pKa = 4.6DD383 pKa = 5.35CKK385 pKa = 11.23DD386 pKa = 3.43ISDD389 pKa = 4.29LKK391 pKa = 10.97QYY393 pKa = 11.3DD394 pKa = 3.37SDD396 pKa = 4.03EE397 pKa = 4.52PEE399 pKa = 4.25SRR401 pKa = 11.84SLASWIQNEE410 pKa = 4.05FNKK413 pKa = 10.52ACEE416 pKa = 4.39LTDD419 pKa = 3.77SSWIEE424 pKa = 3.62LDD426 pKa = 4.1EE427 pKa = 5.66IGEE430 pKa = 4.15DD431 pKa = 3.56VAPIEE436 pKa = 5.34HH437 pKa = 6.74IASMRR442 pKa = 11.84RR443 pKa = 11.84NYY445 pKa = 8.17FTAEE449 pKa = 3.92VSHH452 pKa = 6.6CRR454 pKa = 11.84ATEE457 pKa = 3.87YY458 pKa = 10.63IMKK461 pKa = 10.18GVYY464 pKa = 10.04INTALLNASCAAMDD478 pKa = 4.67DD479 pKa = 4.09FQLIPMISKK488 pKa = 10.11CRR490 pKa = 11.84TKK492 pKa = 10.51EE493 pKa = 3.85GRR495 pKa = 11.84PKK497 pKa = 9.22TNLYY501 pKa = 10.81GFIIKK506 pKa = 9.94GRR508 pKa = 11.84SHH510 pKa = 7.02LRR512 pKa = 11.84NDD514 pKa = 3.25TDD516 pKa = 3.55VVNFVSMEE524 pKa = 4.12FSLTDD529 pKa = 3.59PRR531 pKa = 11.84LEE533 pKa = 3.7PHH535 pKa = 6.34KK536 pKa = 10.03WEE538 pKa = 4.59KK539 pKa = 11.01YY540 pKa = 9.0CVLEE544 pKa = 4.19IGNMLLRR551 pKa = 11.84TAIGQVSRR559 pKa = 11.84PMFLYY564 pKa = 10.52VRR566 pKa = 11.84TNGTSKK572 pKa = 10.61IKK574 pKa = 9.96MKK576 pKa = 9.82WGMEE580 pKa = 3.71MRR582 pKa = 11.84RR583 pKa = 11.84CLLQSLQQIEE593 pKa = 4.49SMIEE597 pKa = 3.67AEE599 pKa = 4.44SSIKK603 pKa = 10.3EE604 pKa = 3.68KK605 pKa = 11.36DD606 pKa = 3.08MTKK609 pKa = 10.61EE610 pKa = 3.6FFEE613 pKa = 4.76NKK615 pKa = 9.96SEE617 pKa = 3.88TWPIGEE623 pKa = 4.22SPKK626 pKa = 10.75GVEE629 pKa = 4.03EE630 pKa = 4.32GSIGKK635 pKa = 8.56VCRR638 pKa = 11.84TLLAKK643 pKa = 10.3SVFNSLYY650 pKa = 10.64ASPQLEE656 pKa = 4.04GFQLSQEE663 pKa = 4.0ITSIVQALRR672 pKa = 11.84DD673 pKa = 3.79NLEE676 pKa = 4.4PGTFDD681 pKa = 5.09LGGLYY686 pKa = 9.85EE687 pKa = 5.25AIEE690 pKa = 4.38EE691 pKa = 4.37CLINDD696 pKa = 3.63PWVLLNASWFNSFLTHH712 pKa = 6.92ALKK715 pKa = 10.78

Molecular weight: 82.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5Q155|Q5Q155_9INFA Polymerase acidic protein OS=Influenza A virus (A/swine/Korea/S452/2004(H9N2)) OX=300749 GN=PA PE=3 SV=1
SS1 pKa = 7.07KK2 pKa = 10.44SRR4 pKa = 11.84SIIFNMEE11 pKa = 4.05RR12 pKa = 11.84IKK14 pKa = 10.54EE15 pKa = 4.02LRR17 pKa = 11.84DD18 pKa = 3.29LMSQSRR24 pKa = 11.84TRR26 pKa = 11.84EE27 pKa = 3.86ILTKK31 pKa = 8.81TTVDD35 pKa = 2.86HH36 pKa = 5.76MAIIKK41 pKa = 10.06KK42 pKa = 7.84YY43 pKa = 8.29TSGRR47 pKa = 11.84QEE49 pKa = 3.99KK50 pKa = 10.64NPALRR55 pKa = 11.84MKK57 pKa = 10.19WMMAMKK63 pKa = 10.45YY64 pKa = 10.07PITADD69 pKa = 3.19KK70 pKa = 10.67RR71 pKa = 11.84IMEE74 pKa = 4.94MIPEE78 pKa = 4.3RR79 pKa = 11.84NEE81 pKa = 3.52QGQTLWSKK89 pKa = 9.91TNDD92 pKa = 3.41AGSDD96 pKa = 3.25KK97 pKa = 11.49VMVSPLAVTWWNRR110 pKa = 11.84NGPTTSTIHH119 pKa = 5.48YY120 pKa = 7.04PKK122 pKa = 10.33VYY124 pKa = 8.46KK125 pKa = 10.24TYY127 pKa = 9.43FEE129 pKa = 4.01KK130 pKa = 11.11VEE132 pKa = 4.08RR133 pKa = 11.84LKK135 pKa = 11.08HH136 pKa = 4.83GTFGPIHH143 pKa = 6.46FRR145 pKa = 11.84NQVKK149 pKa = 9.56IRR151 pKa = 11.84RR152 pKa = 11.84RR153 pKa = 11.84VDD155 pKa = 3.35TNPGHH160 pKa = 7.41ADD162 pKa = 3.79LSAKK166 pKa = 9.4EE167 pKa = 3.98AQDD170 pKa = 3.52VIMEE174 pKa = 4.23VVFPNEE180 pKa = 3.21VGARR184 pKa = 11.84ILTSEE189 pKa = 4.22SQLTITKK196 pKa = 9.33EE197 pKa = 4.15KK198 pKa = 10.77KK199 pKa = 10.27EE200 pKa = 3.94EE201 pKa = 3.95LQDD204 pKa = 3.83CKK206 pKa = 10.5IAPLMVAYY214 pKa = 9.47MLEE217 pKa = 4.18RR218 pKa = 11.84EE219 pKa = 4.67LVRR222 pKa = 11.84KK223 pKa = 7.77TRR225 pKa = 11.84FLPVAGGTSSVYY237 pKa = 10.37IEE239 pKa = 4.48VLHH242 pKa = 5.98LTQGTCWEE250 pKa = 4.0QMYY253 pKa = 9.43TPGGEE258 pKa = 4.01VRR260 pKa = 11.84NDD262 pKa = 3.78DD263 pKa = 3.61VDD265 pKa = 3.41QSLIIAARR273 pKa = 11.84NIVRR277 pKa = 11.84RR278 pKa = 11.84ATVSADD284 pKa = 3.25PLASLLEE291 pKa = 4.39MCHH294 pKa = 5.41GTQIGGIRR302 pKa = 11.84MVDD305 pKa = 3.44ILRR308 pKa = 11.84QNPTEE313 pKa = 3.98EE314 pKa = 4.03QAVDD318 pKa = 2.95ICKK321 pKa = 10.24AAMGLRR327 pKa = 11.84ISSSFSFGGFTFKK340 pKa = 10.05RR341 pKa = 11.84TSGASVKK348 pKa = 10.55KK349 pKa = 10.29EE350 pKa = 3.84EE351 pKa = 4.24EE352 pKa = 4.22VLTGNLQTLKK362 pKa = 10.51ISVHH366 pKa = 5.45EE367 pKa = 4.8GYY369 pKa = 10.87EE370 pKa = 3.95EE371 pKa = 3.84FTMVGRR377 pKa = 11.84RR378 pKa = 11.84ATALLRR384 pKa = 11.84KK385 pKa = 8.11ATRR388 pKa = 11.84RR389 pKa = 11.84LIQLIVSGRR398 pKa = 11.84DD399 pKa = 3.25EE400 pKa = 3.97QSITEE405 pKa = 4.46AIIVAMVFSQEE416 pKa = 3.62DD417 pKa = 3.9CMIKK421 pKa = 10.33AVRR424 pKa = 11.84GDD426 pKa = 3.68LNFVNRR432 pKa = 11.84ANQRR436 pKa = 11.84LNPMHH441 pKa = 6.37QLLRR445 pKa = 11.84HH446 pKa = 5.04FQKK449 pKa = 10.58DD450 pKa = 3.4AKK452 pKa = 11.02VLFQNWGIEE461 pKa = 4.2PIDD464 pKa = 3.91NVMGMIGILPDD475 pKa = 3.56MTPSTEE481 pKa = 3.52MSLRR485 pKa = 11.84GVRR488 pKa = 11.84VSKK491 pKa = 10.41MGVDD495 pKa = 4.11EE496 pKa = 4.4YY497 pKa = 11.53SSTEE501 pKa = 3.91RR502 pKa = 11.84VVVSIDD508 pKa = 2.74RR509 pKa = 11.84FLRR512 pKa = 11.84VRR514 pKa = 11.84DD515 pKa = 3.41QRR517 pKa = 11.84GNILLSPEE525 pKa = 4.37EE526 pKa = 3.98VSEE529 pKa = 4.32TQGTEE534 pKa = 3.64KK535 pKa = 10.4LTITYY540 pKa = 9.18SSSMMWEE547 pKa = 3.7INGPEE552 pKa = 3.98SVLVNTYY559 pKa = 7.57QWIIRR564 pKa = 11.84NWEE567 pKa = 3.94TVKK570 pKa = 10.56IQWSQDD576 pKa = 2.67PTMLYY581 pKa = 10.5NKK583 pKa = 9.92VEE585 pKa = 4.18FEE587 pKa = 4.34PFQSLVPKK595 pKa = 10.24AARR598 pKa = 11.84GQYY601 pKa = 10.02SGFVRR606 pKa = 11.84TLFQQMRR613 pKa = 11.84DD614 pKa = 3.48VLGTFDD620 pKa = 3.43TVQIIKK626 pKa = 10.36LLPFAAAPPEE636 pKa = 3.92QSRR639 pKa = 11.84MQFSSLTVNVRR650 pKa = 11.84GSGMRR655 pKa = 11.84ILVRR659 pKa = 11.84GNSPVFNYY667 pKa = 10.56NKK669 pKa = 8.71ATKK672 pKa = 10.14RR673 pKa = 11.84LTVLGKK679 pKa = 10.13DD680 pKa = 3.2AGALTEE686 pKa = 5.33DD687 pKa = 4.67PDD689 pKa = 3.58EE690 pKa = 4.54GTAGVEE696 pKa = 4.29SAVLRR701 pKa = 11.84GFLILGKK708 pKa = 9.47EE709 pKa = 4.26DD710 pKa = 3.46KK711 pKa = 10.5RR712 pKa = 11.84YY713 pKa = 10.65GPALSINEE721 pKa = 4.34LSNLAKK727 pKa = 10.63GEE729 pKa = 4.09KK730 pKa = 10.32ANVLIGQGDD739 pKa = 3.9VVLVMKK745 pKa = 10.39RR746 pKa = 11.84KK747 pKa = 9.29RR748 pKa = 11.84DD749 pKa = 3.62SSILTDD755 pKa = 3.31SQTATKK761 pKa = 9.73RR762 pKa = 11.84IRR764 pKa = 11.84MAINN768 pKa = 3.15

Molecular weight: 86.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

4250

230

768

531.3

60.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.929 ± 0.455

1.859 ± 0.451

4.659 ± 0.202

7.176 ± 0.744

3.718 ± 0.291

6.847 ± 0.463

1.671 ± 0.147

6.612 ± 0.307

5.812 ± 0.405

7.788 ± 0.498

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.765 ± 0.393

5.388 ± 0.541

3.882 ± 0.198

3.953 ± 0.277

6.635 ± 0.648

7.459 ± 0.442

6.706 ± 0.366

5.788 ± 0.52

1.6 ± 0.175

2.753 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski