Amino acid dipepetide frequency for Ruminococcaceae bacterium CPB6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.813AlaAla: 11.813 ± 0.21	1.602AlaCys: 1.602 ± 0.055	5.349AlaAsp: 5.349 ± 0.102	5.531AlaGlu: 5.531 ± 0.103	3.538AlaPhe: 3.538 ± 0.078	7.476AlaGly: 7.476 ± 0.127	1.622AlaHis: 1.622 ± 0.057	4.38AlaIle: 4.38 ± 0.1	4.914AlaLys: 4.914 ± 0.098	8.644AlaLeu: 8.644 ± 0.141	2.533AlaMet: 2.533 ± 0.073	2.671AlaAsn: 2.671 ± 0.073	3.049AlaPro: 3.049 ± 0.083	3.606AlaGln: 3.606 ± 0.089	3.909AlaArg: 3.909 ± 0.094	4.871AlaSer: 4.871 ± 0.126	3.542AlaThr: 3.542 ± 0.078	8.173AlaVal: 8.173 ± 0.152	0.621AlaTrp: 0.621 ± 0.034	2.64AlaTyr: 2.64 ± 0.07	0.0AlaXaa: 0.0 ± 0.0
Cys	1.683CysAla: 1.683 ± 0.059	0.457CysCys: 0.457 ± 0.031	0.991CysAsp: 0.991 ± 0.039	0.85CysGlu: 0.85 ± 0.04	0.741CysPhe: 0.741 ± 0.034	2.018CysGly: 2.018 ± 0.066	0.418CysHis: 0.418 ± 0.028	1.034CysIle: 1.034 ± 0.046	0.801CysLys: 0.801 ± 0.04	1.389CysLeu: 1.389 ± 0.047	0.5CysMet: 0.5 ± 0.032	0.527CysAsn: 0.527 ± 0.029	0.922CysPro: 0.922 ± 0.048	0.57CysGln: 0.57 ± 0.033	1.194CysArg: 1.194 ± 0.045	1.219CysSer: 1.219 ± 0.054	1.102CysThr: 1.102 ± 0.044	1.2CysVal: 1.2 ± 0.049	0.15CysTrp: 0.15 ± 0.017	0.5CysTyr: 0.5 ± 0.027	0.0CysXaa: 0.0 ± 0.0
Asp	4.625AspAla: 4.625 ± 0.086	0.956AspCys: 0.956 ± 0.04	2.695AspAsp: 2.695 ± 0.074	3.638AspGlu: 3.638 ± 0.087	2.372AspPhe: 2.372 ± 0.066	4.37AspGly: 4.37 ± 0.098	1.098AspHis: 1.098 ± 0.048	3.78AspIle: 3.78 ± 0.084	2.994AspLys: 2.994 ± 0.081	4.716AspLeu: 4.716 ± 0.101	1.741AspMet: 1.741 ± 0.054	1.845AspAsn: 1.845 ± 0.069	2.047AspPro: 2.047 ± 0.068	1.348AspGln: 1.348 ± 0.044	2.763AspArg: 2.763 ± 0.078	3.312AspSer: 3.312 ± 0.076	3.4AspThr: 3.4 ± 0.09	3.548AspVal: 3.548 ± 0.078	0.568AspTrp: 0.568 ± 0.037	2.118AspTyr: 2.118 ± 0.066	0.0AspXaa: 0.0 ± 0.0
Glu	4.881GluAla: 4.881 ± 0.089	0.714GluCys: 0.714 ± 0.036	3.113GluAsp: 3.113 ± 0.086	4.521GluGlu: 4.521 ± 0.11	1.673GluPhe: 1.673 ± 0.055	3.44GluGly: 3.44 ± 0.081	1.236GluHis: 1.236 ± 0.05	3.718GluIle: 3.718 ± 0.087	5.107GluLys: 5.107 ± 0.102	5.492GluLeu: 5.492 ± 0.109	1.911GluMet: 1.911 ± 0.059	3.168GluAsn: 3.168 ± 0.073	2.096GluPro: 2.096 ± 0.061	2.872GluGln: 2.872 ± 0.071	3.011GluArg: 3.011 ± 0.078	2.97GluSer: 2.97 ± 0.07	3.169GluThr: 3.169 ± 0.07	3.577GluVal: 3.577 ± 0.082	0.462GluTrp: 0.462 ± 0.033	1.727GluTyr: 1.727 ± 0.058	0.0GluXaa: 0.0 ± 0.0
Phe	3.307PheAla: 3.307 ± 0.085	0.888PheCys: 0.888 ± 0.043	2.139PheAsp: 2.139 ± 0.06	1.79PheGlu: 1.79 ± 0.056	1.709PhePhe: 1.709 ± 0.066	3.159PheGly: 3.159 ± 0.078	0.976PheHis: 0.976 ± 0.044	2.164PheIle: 2.164 ± 0.065	1.389PheLys: 1.389 ± 0.054	3.945PheLeu: 3.945 ± 0.089	0.956PheMet: 0.956 ± 0.047	1.234PheAsn: 1.234 ± 0.052	1.629PhePro: 1.629 ± 0.054	1.413PheGln: 1.413 ± 0.052	1.807PheArg: 1.807 ± 0.057	3.198PheSer: 3.198 ± 0.075	2.484PheThr: 2.484 ± 0.071	2.533PheVal: 2.533 ± 0.072	0.372PheTrp: 0.372 ± 0.028	1.455PheTyr: 1.455 ± 0.052	0.0PheXaa: 0.0 ± 0.0
Gly	5.932GlyAla: 5.932 ± 0.124	1.615GlyCys: 1.615 ± 0.051	3.494GlyAsp: 3.494 ± 0.08	3.846GlyGlu: 3.846 ± 0.081	2.953GlyPhe: 2.953 ± 0.073	5.75GlyGly: 5.75 ± 0.118	1.554GlyHis: 1.554 ± 0.05	5.516GlyIle: 5.516 ± 0.123	5.271GlyLys: 5.271 ± 0.087	6.471GlyLeu: 6.471 ± 0.125	2.408GlyMet: 2.408 ± 0.064	2.612GlyAsn: 2.612 ± 0.076	1.884GlyPro: 1.884 ± 0.065	2.51GlyGln: 2.51 ± 0.084	4.019GlyArg: 4.019 ± 0.098	4.773GlySer: 4.773 ± 0.098	4.941GlyThr: 4.941 ± 0.104	5.347GlyVal: 5.347 ± 0.102	0.78GlyTrp: 0.78 ± 0.034	2.712GlyTyr: 2.712 ± 0.073	0.0GlyXaa: 0.0 ± 0.0
His	1.605HisAla: 1.605 ± 0.061	0.493HisCys: 0.493 ± 0.032	1.052HisAsp: 1.052 ± 0.04	0.913HisGlu: 0.913 ± 0.039	0.996HisPhe: 0.996 ± 0.044	1.687HisGly: 1.687 ± 0.052	0.527HisHis: 0.527 ± 0.035	1.42HisIle: 1.42 ± 0.046	1.046HisLys: 1.046 ± 0.044	2.008HisLeu: 2.008 ± 0.065	0.536HisMet: 0.536 ± 0.031	0.765HisAsn: 0.765 ± 0.038	1.175HisPro: 1.175 ± 0.045	0.639HisGln: 0.639 ± 0.041	1.188HisArg: 1.188 ± 0.047	1.255HisSer: 1.255 ± 0.048	1.46HisThr: 1.46 ± 0.052	1.409HisVal: 1.409 ± 0.049	0.255HisTrp: 0.255 ± 0.02	0.692HisTyr: 0.692 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	5.325IleAla: 5.325 ± 0.099	1.221IleCys: 1.221 ± 0.044	3.436IleAsp: 3.436 ± 0.09	2.884IleGlu: 2.884 ± 0.087	2.282IlePhe: 2.282 ± 0.073	4.302IleGly: 4.302 ± 0.111	1.294IleHis: 1.294 ± 0.051	3.501IleIle: 3.501 ± 0.093	2.654IleLys: 2.654 ± 0.087	5.918IleLeu: 5.918 ± 0.124	1.423IleMet: 1.423 ± 0.05	2.078IleAsn: 2.078 ± 0.06	3.015IlePro: 3.015 ± 0.077	2.164IleGln: 2.164 ± 0.062	3.166IleArg: 3.166 ± 0.074	4.329IleSer: 4.329 ± 0.095	3.632IleThr: 3.632 ± 0.078	4.055IleVal: 4.055 ± 0.097	0.517IleTrp: 0.517 ± 0.033	1.96IleTyr: 1.96 ± 0.065	0.0IleXaa: 0.0 ± 0.0
Lys	5.306LysAla: 5.306 ± 0.108	0.687LysCys: 0.687 ± 0.035	3.332LysAsp: 3.332 ± 0.079	4.4LysGlu: 4.4 ± 0.095	1.389LysPhe: 1.389 ± 0.045	3.616LysGly: 3.616 ± 0.09	1.078LysHis: 1.078 ± 0.043	3.693LysIle: 3.693 ± 0.092	4.803LysLys: 4.803 ± 0.095	4.825LysLeu: 4.825 ± 0.105	1.998LysMet: 1.998 ± 0.056	2.787LysAsn: 2.787 ± 0.077	2.294LysPro: 2.294 ± 0.063	2.367LysGln: 2.367 ± 0.073	2.766LysArg: 2.766 ± 0.067	3.149LysSer: 3.149 ± 0.086	3.705LysThr: 3.705 ± 0.083	3.912LysVal: 3.912 ± 0.082	0.512LysTrp: 0.512 ± 0.031	1.972LysTyr: 1.972 ± 0.058	0.0LysXaa: 0.0 ± 0.0
Leu	7.964LeuAla: 7.964 ± 0.116	2.149LeuCys: 2.149 ± 0.072	4.851LeuAsp: 4.851 ± 0.089	5.019LeuGlu: 5.019 ± 0.106	4.006LeuPhe: 4.006 ± 0.11	6.284LeuGly: 6.284 ± 0.096	2.34LeuHis: 2.34 ± 0.058	4.808LeuIle: 4.808 ± 0.1	5.031LeuLys: 5.031 ± 0.1	9.994LeuLeu: 9.994 ± 0.19	2.498LeuMet: 2.498 ± 0.073	3.472LeuAsn: 3.472 ± 0.073	4.854LeuPro: 4.854 ± 0.095	4.468LeuGln: 4.468 ± 0.089	5.148LeuArg: 5.148 ± 0.099	6.497LeuSer: 6.497 ± 0.137	5.765LeuThr: 5.765 ± 0.094	5.713LeuVal: 5.713 ± 0.097	0.838LeuTrp: 0.838 ± 0.039	3.106LeuTyr: 3.106 ± 0.083	0.0LeuXaa: 0.0 ± 0.0
Met	2.586MetAla: 2.586 ± 0.073	0.36MetCys: 0.36 ± 0.025	1.687MetAsp: 1.687 ± 0.058	1.843MetGlu: 1.843 ± 0.054	0.786MetPhe: 0.786 ± 0.032	1.993MetGly: 1.993 ± 0.061	0.568MetHis: 0.568 ± 0.035	1.447MetIle: 1.447 ± 0.053	2.071MetLys: 2.071 ± 0.059	2.844MetLeu: 2.844 ± 0.089	0.743MetMet: 0.743 ± 0.039	1.296MetAsn: 1.296 ± 0.047	1.326MetPro: 1.326 ± 0.046	1.401MetGln: 1.401 ± 0.05	1.472MetArg: 1.472 ± 0.044	1.517MetSer: 1.517 ± 0.055	1.784MetThr: 1.784 ± 0.066	1.858MetVal: 1.858 ± 0.061	0.163MetTrp: 0.163 ± 0.017	0.741MetTyr: 0.741 ± 0.034	0.0MetXaa: 0.0 ± 0.0
Asn	3.417AsnAla: 3.417 ± 0.083	0.675AsnCys: 0.675 ± 0.036	1.872AsnAsp: 1.872 ± 0.053	1.942AsnGlu: 1.942 ± 0.06	1.358AsnPhe: 1.358 ± 0.05	3.526AsnGly: 3.526 ± 0.082	0.772AsnHis: 0.772 ± 0.039	2.491AsnIle: 2.491 ± 0.074	1.823AsnLys: 1.823 ± 0.07	3.429AsnLeu: 3.429 ± 0.083	1.068AsnMet: 1.068 ± 0.043	1.301AsnAsn: 1.301 ± 0.046	2.005AsnPro: 2.005 ± 0.057	1.212AsnGln: 1.212 ± 0.054	1.914AsnArg: 1.914 ± 0.054	2.156AsnSer: 2.156 ± 0.073	2.132AsnThr: 2.132 ± 0.055	2.63AsnVal: 2.63 ± 0.066	0.388AsnTrp: 0.388 ± 0.028	1.35AsnTyr: 1.35 ± 0.055	0.0AsnXaa: 0.0 ± 0.0
Pro	3.878ProAla: 3.878 ± 0.089	0.678ProCys: 0.678 ± 0.038	2.846ProAsp: 2.846 ± 0.068	3.351ProGlu: 3.351 ± 0.078	1.704ProPhe: 1.704 ± 0.061	2.912ProGly: 2.912 ± 0.074	0.865ProHis: 0.865 ± 0.037	2.127ProIle: 2.127 ± 0.071	2.272ProLys: 2.272 ± 0.069	3.678ProLeu: 3.678 ± 0.095	1.069ProMet: 1.069 ± 0.043	1.547ProAsn: 1.547 ± 0.058	1.457ProPro: 1.457 ± 0.054	1.986ProGln: 1.986 ± 0.063	1.648ProArg: 1.648 ± 0.054	2.173ProSer: 2.173 ± 0.058	2.059ProThr: 2.059 ± 0.062	3.489ProVal: 3.489 ± 0.084	0.384ProTrp: 0.384 ± 0.026	1.488ProTyr: 1.488 ± 0.051	0.0ProXaa: 0.0 ± 0.0
Gln	3.463GlnAla: 3.463 ± 0.084	0.471GlnCys: 0.471 ± 0.033	1.826GlnAsp: 1.826 ± 0.053	2.476GlnGlu: 2.476 ± 0.056	1.33GlnPhe: 1.33 ± 0.045	2.156GlnGly: 2.156 ± 0.069	0.818GlnHis: 0.818 ± 0.038	2.391GlnIle: 2.391 ± 0.055	3.48GlnLys: 3.48 ± 0.095	3.775GlnLeu: 3.775 ± 0.094	1.44GlnMet: 1.44 ± 0.05	1.882GlnAsn: 1.882 ± 0.061	1.644GlnPro: 1.644 ± 0.051	2.345GlnGln: 2.345 ± 0.082	2.039GlnArg: 2.039 ± 0.059	1.923GlnSer: 1.923 ± 0.058	2.34GlnThr: 2.34 ± 0.08	2.552GlnVal: 2.552 ± 0.073	0.357GlnTrp: 0.357 ± 0.025	1.433GlnTyr: 1.433 ± 0.055	0.0GlnXaa: 0.0 ± 0.0
Arg	3.909ArgAla: 3.909 ± 0.092	0.933ArgCys: 0.933 ± 0.039	2.423ArgAsp: 2.423 ± 0.083	3.351ArgGlu: 3.351 ± 0.087	2.176ArgPhe: 2.176 ± 0.068	3.152ArgGly: 3.152 ± 0.077	1.078ArgHis: 1.078 ± 0.042	3.331ArgIle: 3.331 ± 0.085	3.239ArgLys: 3.239 ± 0.077	4.985ArgLeu: 4.985 ± 0.103	1.6ArgMet: 1.6 ± 0.05	1.829ArgAsn: 1.829 ± 0.067	2.027ArgPro: 2.027 ± 0.065	2.3ArgGln: 2.3 ± 0.069	3.572ArgArg: 3.572 ± 0.108	2.88ArgSer: 2.88 ± 0.078	2.916ArgThr: 2.916 ± 0.075	3.37ArgVal: 3.37 ± 0.076	0.568ArgTrp: 0.568 ± 0.032	1.855ArgTyr: 1.855 ± 0.058	0.0ArgXaa: 0.0 ± 0.0
Ser	5.963SerAla: 5.963 ± 0.124	1.115SerCys: 1.115 ± 0.042	3.159SerAsp: 3.159 ± 0.09	3.118SerGlu: 3.118 ± 0.076	2.676SerPhe: 2.676 ± 0.06	5.711SerGly: 5.711 ± 0.105	1.256SerHis: 1.256 ± 0.049	3.535SerIle: 3.535 ± 0.078	3.013SerLys: 3.013 ± 0.081	5.611SerLeu: 5.611 ± 0.114	1.695SerMet: 1.695 ± 0.05	2.01SerAsn: 2.01 ± 0.069	2.391SerPro: 2.391 ± 0.062	2.079SerGln: 2.079 ± 0.064	3.278SerArg: 3.278 ± 0.083	4.674SerSer: 4.674 ± 0.164	3.409SerThr: 3.409 ± 0.101	4.56SerVal: 4.56 ± 0.098	0.609SerTrp: 0.609 ± 0.037	2.052SerTyr: 2.052 ± 0.068	0.0SerXaa: 0.0 ± 0.0
Thr	6.289ThrAla: 6.289 ± 0.129	0.916ThrCys: 0.916 ± 0.042	3.433ThrAsp: 3.433 ± 0.084	3.297ThrGlu: 3.297 ± 0.071	2.285ThrPhe: 2.285 ± 0.067	5.012ThrGly: 5.012 ± 0.102	1.148ThrHis: 1.148 ± 0.045	3.31ThrIle: 3.31 ± 0.092	2.697ThrLys: 2.697 ± 0.067	5.488ThrLeu: 5.488 ± 0.097	1.411ThrMet: 1.411 ± 0.044	2.047ThrAsn: 2.047 ± 0.057	2.69ThrPro: 2.69 ± 0.079	1.943ThrGln: 1.943 ± 0.066	2.263ThrArg: 2.263 ± 0.068	3.21ThrSer: 3.21 ± 0.084	2.926ThrThr: 2.926 ± 0.086	4.994ThrVal: 4.994 ± 0.117	0.481ThrTrp: 0.481 ± 0.033	1.838ThrTyr: 1.838 ± 0.064	0.0ThrXaa: 0.0 ± 0.0
Val	5.104ValAla: 5.104 ± 0.099	1.488ValCys: 1.488 ± 0.05	3.557ValAsp: 3.557 ± 0.088	3.701ValGlu: 3.701 ± 0.085	2.771ValPhe: 2.771 ± 0.073	4.572ValGly: 4.572 ± 0.116	1.433ValHis: 1.433 ± 0.054	4.089ValIle: 4.089 ± 0.085	3.742ValLys: 3.742 ± 0.084	7.704ValLeu: 7.704 ± 0.127	1.841ValMet: 1.841 ± 0.057	2.562ValAsn: 2.562 ± 0.061	3.457ValPro: 3.457 ± 0.087	3.033ValGln: 3.033 ± 0.085	3.771ValArg: 3.771 ± 0.082	5.201ValSer: 5.201 ± 0.109	4.393ValThr: 4.393 ± 0.088	4.718ValVal: 4.718 ± 0.105	0.729ValTrp: 0.729 ± 0.033	2.316ValTyr: 2.316 ± 0.072	0.0ValXaa: 0.0 ± 0.0
Trp	0.675TrpAla: 0.675 ± 0.038	0.197TrpCys: 0.197 ± 0.02	0.58TrpAsp: 0.58 ± 0.035	0.571TrpGlu: 0.571 ± 0.031	0.394TrpPhe: 0.394 ± 0.029	0.612TrpGly: 0.612 ± 0.032	0.211TrpHis: 0.211 ± 0.02	0.544TrpIle: 0.544 ± 0.031	0.602TrpLys: 0.602 ± 0.032	0.85TrpLeu: 0.85 ± 0.038	0.315TrpMet: 0.315 ± 0.024	0.505TrpAsn: 0.505 ± 0.037	0.252TrpPro: 0.252 ± 0.022	0.496TrpGln: 0.496 ± 0.031	0.445TrpArg: 0.445 ± 0.031	0.527TrpSer: 0.527 ± 0.034	0.418TrpThr: 0.418 ± 0.029	0.547TrpVal: 0.547 ± 0.03	0.095TrpTrp: 0.095 ± 0.014	0.364TrpTyr: 0.364 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.756TyrAla: 2.756 ± 0.075	0.649TyrCys: 0.649 ± 0.031	2.1TyrAsp: 2.1 ± 0.061	1.818TyrGlu: 1.818 ± 0.056	1.408TyrPhe: 1.408 ± 0.052	2.639TyrGly: 2.639 ± 0.063	0.83TyrHis: 0.83 ± 0.04	1.877TyrIle: 1.877 ± 0.064	1.644TyrLys: 1.644 ± 0.059	3.038TyrLeu: 3.038 ± 0.081	0.799TyrMet: 0.799 ± 0.039	1.324TyrAsn: 1.324 ± 0.051	1.401TyrPro: 1.401 ± 0.047	1.386TyrGln: 1.386 ± 0.047	2.122TyrArg: 2.122 ± 0.072	2.081TyrSer: 2.081 ± 0.064	2.101TyrThr: 2.101 ± 0.069	1.996TyrVal: 1.996 ± 0.058	0.352TyrTrp: 0.352 ± 0.029	1.358TyrTyr: 1.358 ± 0.06	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 1921 proteins (588157 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---