Diatraea saccharalis granulovirus
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 125 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R7EYV4|A0A0R7EYV4_9BBAC Uncharacterized protein OS=Diatraea saccharalis granulovirus OX=1675862 GN=ORF-11 PE=4 SV=1
MM1 pKa = 7.57 EE2 pKa = 5.65 SDD4 pKa = 4.41 EE5 pKa = 5.45 SILDD9 pKa = 4.05 NILKK13 pKa = 10.86 DD14 pKa = 4.03 NVRR17 pKa = 11.84 LLNNKK22 pKa = 8.55 YY23 pKa = 10.76 LIFNVVDD30 pKa = 3.47 IDD32 pKa = 4.09 DD33 pKa = 4.21 EE34 pKa = 4.68 LRR36 pKa = 11.84 TSCYY40 pKa = 10.64 GILNLPTDD48 pKa = 4.04 TNQQEE53 pKa = 4.59 TLSSSSQSGEE63 pKa = 4.12 LYY65 pKa = 10.71 SDD67 pKa = 3.63 TNEE70 pKa = 3.99 GTNKK74 pKa = 9.99 NNQQ77 pKa = 3.0
Molecular weight: 8.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.159
IPC2_protein 3.923
IPC_protein 3.834
Toseland 3.643
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.77
Rodwell 3.668
Grimsley 3.554
Solomon 3.808
Lehninger 3.77
Nozaki 3.961
DTASelect 4.151
Thurlkill 3.706
EMBOSS 3.783
Sillero 3.961
Patrickios 3.389
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.881
Protein with the highest isoelectric point:
>tr|A0A0R7EYT3|A0A0R7EYT3_9BBAC Uncharacterized protein OS=Diatraea saccharalis granulovirus OX=1675862 GN=ORF-73 PE=4 SV=1
MM1 pKa = 6.52 TRR3 pKa = 11.84 RR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 SLRR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 SRR13 pKa = 11.84 SPRR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 RR19 pKa = 11.84 SRR21 pKa = 11.84 SPTQHH26 pKa = 4.86 YY27 pKa = 9.71 RR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 SRR32 pKa = 11.84 SRR34 pKa = 11.84 STSPYY39 pKa = 8.58 RR40 pKa = 11.84 RR41 pKa = 11.84 RR42 pKa = 11.84 SRR44 pKa = 11.84 SRR46 pKa = 11.84 SRR48 pKa = 11.84 SPYY51 pKa = 7.33 RR52 pKa = 11.84 RR53 pKa = 11.84 RR54 pKa = 11.84 YY55 pKa = 9.11 SRR57 pKa = 11.84 HH58 pKa = 4.42 EE59 pKa = 3.84 QVV61 pKa = 3.45
Molecular weight: 7.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.375
IPC2_protein 11.111
IPC_protein 12.413
Toseland 12.559
ProMoST 13.071
Dawson 12.559
Bjellqvist 12.559
Wikipedia 13.042
Rodwell 12.062
Grimsley 12.603
Solomon 13.071
Lehninger 12.969
Nozaki 12.559
DTASelect 12.559
Thurlkill 12.559
EMBOSS 13.071
Sillero 12.559
Patrickios 11.784
IPC_peptide 13.071
IPC2_peptide 12.062
IPC2.peptide.svr19 9.137
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
125
0
125
31082
49
1127
248.7
29.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.201 ± 0.163
2.513 ± 0.126
6.155 ± 0.148
6.666 ± 0.274
5.46 ± 0.203
3.748 ± 0.174
2.133 ± 0.081
7.339 ± 0.185
7.779 ± 0.337
9.539 ± 0.172
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.725 ± 0.128
7.915 ± 0.185
3.272 ± 0.296
2.76 ± 0.101
4.717 ± 0.151
5.569 ± 0.165
5.778 ± 0.172
6.65 ± 0.175
1.097 ± 0.082
4.964 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here