Pepper yellow vein Mali virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q6RCV0|Q6RCV0_9GEMI Protein V2 OS=Pepper yellow vein Mali virus OX=260378 GN=V1 PE=3 SV=1
MM1 pKa = 8.06WDD3 pKa = 3.35PLLNEE8 pKa = 4.55FPEE11 pKa = 4.6SVHH14 pKa = 6.55GFRR17 pKa = 11.84CMLAIKK23 pKa = 9.6YY24 pKa = 8.3LQAVEE29 pKa = 4.12EE30 pKa = 4.52TYY32 pKa = 10.93EE33 pKa = 4.29PNTLGHH39 pKa = 6.99DD40 pKa = 5.01LIRR43 pKa = 11.84DD44 pKa = 4.58LISVIRR50 pKa = 11.84ARR52 pKa = 11.84DD53 pKa = 3.49YY54 pKa = 11.1VEE56 pKa = 3.35ATRR59 pKa = 11.84RR60 pKa = 11.84YY61 pKa = 8.17NHH63 pKa = 5.64FHH65 pKa = 6.46ARR67 pKa = 11.84LEE69 pKa = 4.47GSSKK73 pKa = 11.3AEE75 pKa = 3.67LRR77 pKa = 11.84QPIHH81 pKa = 6.29QPCCCLHH88 pKa = 6.35CPRR91 pKa = 11.84HH92 pKa = 5.2KK93 pKa = 10.27QASIMDD99 pKa = 4.35LPAHH103 pKa = 5.71VSKK106 pKa = 9.03TQDD109 pKa = 3.13VQNVQKK115 pKa = 10.64PP116 pKa = 3.36

Molecular weight:
13.49 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q6RCV0|Q6RCV0_9GEMI Protein V2 OS=Pepper yellow vein Mali virus OX=260378 GN=V1 PE=3 SV=1
MM1 pKa = 7.7SKK3 pKa = 10.44RR4 pKa = 11.84PGDD7 pKa = 3.95IIISTPVSKK16 pKa = 10.56VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.04SPYY27 pKa = 8.46TSRR30 pKa = 11.84AAVSIAQGINRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84SWTYY47 pKa = 9.26RR48 pKa = 11.84PMYY51 pKa = 9.54RR52 pKa = 11.84KK53 pKa = 9.31PRR55 pKa = 11.84MYY57 pKa = 11.01RR58 pKa = 11.84MFRR61 pKa = 11.84SPDD64 pKa = 3.23VPRR67 pKa = 11.84GCEE70 pKa = 4.34GPCKK74 pKa = 9.32VQSYY78 pKa = 6.99EE79 pKa = 3.67QRR81 pKa = 11.84DD82 pKa = 4.05DD83 pKa = 3.72VKK85 pKa = 9.5HH86 pKa = 5.48TGIVRR91 pKa = 11.84CVSDD95 pKa = 3.36VTRR98 pKa = 11.84GPGITHH104 pKa = 6.76RR105 pKa = 11.84VGKK108 pKa = 9.68RR109 pKa = 11.84FCIKK113 pKa = 10.26SIYY116 pKa = 9.81ILGKK120 pKa = 9.09IWMDD124 pKa = 3.64EE125 pKa = 4.09NIKK128 pKa = 10.06KK129 pKa = 9.97QNHH132 pKa = 4.95TNQVMFFLVRR142 pKa = 11.84DD143 pKa = 3.69RR144 pKa = 11.84RR145 pKa = 11.84PYY147 pKa = 8.77GTSPQDD153 pKa = 3.2FGQVFNMFDD162 pKa = 3.99NEE164 pKa = 4.11PSTATVKK171 pKa = 10.81NDD173 pKa = 2.84LRR175 pKa = 11.84DD176 pKa = 3.47RR177 pKa = 11.84YY178 pKa = 9.28QVLRR182 pKa = 11.84KK183 pKa = 8.77FHH185 pKa = 5.93ATVVGGPSGMKK196 pKa = 9.47EE197 pKa = 3.54QALVKK202 pKa = 10.43RR203 pKa = 11.84FFRR206 pKa = 11.84VNTHH210 pKa = 4.29VVYY213 pKa = 10.8NHH215 pKa = 5.77QEE217 pKa = 3.45AAKK220 pKa = 10.4YY221 pKa = 9.25EE222 pKa = 4.13NHH224 pKa = 6.46TEE226 pKa = 3.99NALLLYY232 pKa = 7.29MACTHH237 pKa = 7.07ASNPVYY243 pKa = 9.86ATLKK247 pKa = 9.83IRR249 pKa = 11.84IYY251 pKa = 10.7LYY253 pKa = 10.86DD254 pKa = 3.57AVTNN258 pKa = 4.23

Molecular weight:
30.03 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1082

85

354

180.3

20.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.268 ± 0.701

2.218 ± 0.376

4.713 ± 0.499

4.621 ± 0.764

4.529 ± 0.615

5.176 ± 0.454

4.251 ± 0.893

5.545 ± 0.804

5.545 ± 0.859

7.394 ± 1.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.218 ± 0.435

5.823 ± 0.962

6.007 ± 0.646

4.806 ± 0.624

7.116 ± 1.2

7.301 ± 1.076

6.562 ± 0.981

5.823 ± 1.063

1.386 ± 0.236

3.697 ± 0.49

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski