Pepper yellow vein Mali virus
Average proteome isoelectric point is 7.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q6RCV0|Q6RCV0_9GEMI Protein V2 OS=Pepper yellow vein Mali virus OX=260378 GN=V1 PE=3 SV=1
MM1 pKa = 8.06 WDD3 pKa = 3.35 PLLNEE8 pKa = 4.55 FPEE11 pKa = 4.6 SVHH14 pKa = 6.55 GFRR17 pKa = 11.84 CMLAIKK23 pKa = 9.6 YY24 pKa = 8.3 LQAVEE29 pKa = 4.12 EE30 pKa = 4.52 TYY32 pKa = 10.93 EE33 pKa = 4.29 PNTLGHH39 pKa = 6.99 DD40 pKa = 5.01 LIRR43 pKa = 11.84 DD44 pKa = 4.58 LISVIRR50 pKa = 11.84 ARR52 pKa = 11.84 DD53 pKa = 3.49 YY54 pKa = 11.1 VEE56 pKa = 3.35 ATRR59 pKa = 11.84 RR60 pKa = 11.84 YY61 pKa = 8.17 NHH63 pKa = 5.64 FHH65 pKa = 6.46 ARR67 pKa = 11.84 LEE69 pKa = 4.47 GSSKK73 pKa = 11.3 AEE75 pKa = 3.67 LRR77 pKa = 11.84 QPIHH81 pKa = 6.29 QPCCCLHH88 pKa = 6.35 CPRR91 pKa = 11.84 HH92 pKa = 5.2 KK93 pKa = 10.27 QASIMDD99 pKa = 4.35 LPAHH103 pKa = 5.71 VSKK106 pKa = 9.03 TQDD109 pKa = 3.13 VQNVQKK115 pKa = 10.64 PP116 pKa = 3.36
Molecular weight: 13.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.584
IPC2_protein 6.504
IPC_protein 6.722
Toseland 6.766
ProMoST 7.249
Dawson 7.205
Bjellqvist 7.073
Wikipedia 7.176
Rodwell 7.205
Grimsley 6.868
Solomon 7.263
Lehninger 7.278
Nozaki 7.439
DTASelect 7.512
Thurlkill 7.541
EMBOSS 7.571
Sillero 7.629
Patrickios 4.024
IPC_peptide 7.263
IPC2_peptide 7.322
IPC2.peptide.svr19 7.119
Protein with the highest isoelectric point:
>tr|Q6RCV0|Q6RCV0_9GEMI Protein V2 OS=Pepper yellow vein Mali virus OX=260378 GN=V1 PE=3 SV=1
MM1 pKa = 7.7 SKK3 pKa = 10.44 RR4 pKa = 11.84 PGDD7 pKa = 3.95 IIISTPVSKK16 pKa = 10.56 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFDD24 pKa = 3.04 SPYY27 pKa = 8.46 TSRR30 pKa = 11.84 AAVSIAQGINRR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 SWTYY47 pKa = 9.26 RR48 pKa = 11.84 PMYY51 pKa = 9.54 RR52 pKa = 11.84 KK53 pKa = 9.31 PRR55 pKa = 11.84 MYY57 pKa = 11.01 RR58 pKa = 11.84 MFRR61 pKa = 11.84 SPDD64 pKa = 3.23 VPRR67 pKa = 11.84 GCEE70 pKa = 4.34 GPCKK74 pKa = 9.32 VQSYY78 pKa = 6.99 EE79 pKa = 3.67 QRR81 pKa = 11.84 DD82 pKa = 4.05 DD83 pKa = 3.72 VKK85 pKa = 9.5 HH86 pKa = 5.48 TGIVRR91 pKa = 11.84 CVSDD95 pKa = 3.36 VTRR98 pKa = 11.84 GPGITHH104 pKa = 6.76 RR105 pKa = 11.84 VGKK108 pKa = 9.68 RR109 pKa = 11.84 FCIKK113 pKa = 10.26 SIYY116 pKa = 9.81 ILGKK120 pKa = 9.09 IWMDD124 pKa = 3.64 EE125 pKa = 4.09 NIKK128 pKa = 10.06 KK129 pKa = 9.97 QNHH132 pKa = 4.95 TNQVMFFLVRR142 pKa = 11.84 DD143 pKa = 3.69 RR144 pKa = 11.84 RR145 pKa = 11.84 PYY147 pKa = 8.77 GTSPQDD153 pKa = 3.2 FGQVFNMFDD162 pKa = 3.99 NEE164 pKa = 4.11 PSTATVKK171 pKa = 10.81 NDD173 pKa = 2.84 LRR175 pKa = 11.84 DD176 pKa = 3.47 RR177 pKa = 11.84 YY178 pKa = 9.28 QVLRR182 pKa = 11.84 KK183 pKa = 8.77 FHH185 pKa = 5.93 ATVVGGPSGMKK196 pKa = 9.47 EE197 pKa = 3.54 QALVKK202 pKa = 10.43 RR203 pKa = 11.84 FFRR206 pKa = 11.84 VNTHH210 pKa = 4.29 VVYY213 pKa = 10.8 NHH215 pKa = 5.77 QEE217 pKa = 3.45 AAKK220 pKa = 10.4 YY221 pKa = 9.25 EE222 pKa = 4.13 NHH224 pKa = 6.46 TEE226 pKa = 3.99 NALLLYY232 pKa = 7.29 MACTHH237 pKa = 7.07 ASNPVYY243 pKa = 9.86 ATLKK247 pKa = 9.83 IRR249 pKa = 11.84 IYY251 pKa = 10.7 LYY253 pKa = 10.86 DD254 pKa = 3.57 AVTNN258 pKa = 4.23
Molecular weight: 30.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.3
IPC2_protein 9.619
IPC_protein 10.072
Toseland 10.175
ProMoST 9.94
Dawson 10.394
Bjellqvist 10.116
Wikipedia 10.599
Rodwell 10.687
Grimsley 10.482
Solomon 10.438
Lehninger 10.394
Nozaki 10.204
DTASelect 10.101
Thurlkill 10.248
EMBOSS 10.599
Sillero 10.321
Patrickios 10.189
IPC_peptide 10.423
IPC2_peptide 9.063
IPC2.peptide.svr19 8.57
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1082
85
354
180.3
20.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.268 ± 0.701
2.218 ± 0.376
4.713 ± 0.499
4.621 ± 0.764
4.529 ± 0.615
5.176 ± 0.454
4.251 ± 0.893
5.545 ± 0.804
5.545 ± 0.859
7.394 ± 1.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.218 ± 0.435
5.823 ± 0.962
6.007 ± 0.646
4.806 ± 0.624
7.116 ± 1.2
7.301 ± 1.076
6.562 ± 0.981
5.823 ± 1.063
1.386 ± 0.236
3.697 ± 0.49
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here