Geminiviridae sp.
Average proteome isoelectric point is 8.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A385E372|A0A385E372_9GEMI Uncharacterized protein OS=Geminiviridae sp. OX=2202566 PE=4 SV=1
MM1 pKa = 6.58 TTPAGRR7 pKa = 11.84 IGKK10 pKa = 9.38 ARR12 pKa = 11.84 TKK14 pKa = 10.23 EE15 pKa = 3.82 WFQTFPKK22 pKa = 8.93 WAHH25 pKa = 5.72 EE26 pKa = 3.96 DD27 pKa = 3.56 RR28 pKa = 11.84 EE29 pKa = 4.57 KK30 pKa = 10.63 ILQLLIPLGIQSAKK44 pKa = 10.19 VALEE48 pKa = 3.73 HH49 pKa = 7.12 HH50 pKa = 7.03 EE51 pKa = 4.96 DD52 pKa = 3.81 GTPHH56 pKa = 5.87 FHH58 pKa = 7.76 AIYY61 pKa = 10.69 SLTLKK66 pKa = 8.67 KK67 pKa = 9.17 TKK69 pKa = 9.55 PQLVKK74 pKa = 10.52 YY75 pKa = 9.04 FKK77 pKa = 10.66 INLPDD82 pKa = 3.96 DD83 pKa = 4.02 YY84 pKa = 11.31 KK85 pKa = 10.95 RR86 pKa = 11.84 CTVLRR91 pKa = 11.84 ALEE94 pKa = 4.0 PAKK97 pKa = 10.61 SSKK100 pKa = 10.48 QLTNYY105 pKa = 9.96 LDD107 pKa = 4.95 KK108 pKa = 11.26 EE109 pKa = 4.17 DD110 pKa = 4.21 TDD112 pKa = 4.77 YY113 pKa = 11.35 ISYY116 pKa = 7.86 WQPPNLDD123 pKa = 4.28 HH124 pKa = 7.63 LNDD127 pKa = 3.58 LCSRR131 pKa = 11.84 AGLPDD136 pKa = 3.73 YY137 pKa = 9.39 ATLQVQIRR145 pKa = 11.84 QQRR148 pKa = 11.84 AHH150 pKa = 6.48 EE151 pKa = 4.22 RR152 pKa = 11.84 LCSRR156 pKa = 11.84 ALAAVHH162 pKa = 6.11 EE163 pKa = 4.78 KK164 pKa = 10.24 EE165 pKa = 4.64 LSVSNEE171 pKa = 3.2 FRR173 pKa = 11.84 NLLDD177 pKa = 3.82 SFSGTEE183 pKa = 3.8 NLAIMKK189 pKa = 9.71 NEE191 pKa = 3.83 KK192 pKa = 10.84 GIDD195 pKa = 3.5 TVAMFIASCKK205 pKa = 10.37 DD206 pKa = 3.13 VLKK209 pKa = 9.7 YY210 pKa = 9.38 TGYY213 pKa = 11.35
Molecular weight: 24.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.748
IPC2_protein 7.936
IPC_protein 7.892
Toseland 7.995
ProMoST 8.521
Dawson 8.639
Bjellqvist 8.77
Wikipedia 8.624
Rodwell 8.682
Grimsley 7.892
Solomon 8.814
Lehninger 8.799
Nozaki 8.931
DTASelect 8.595
Thurlkill 8.682
EMBOSS 8.829
Sillero 8.931
Patrickios 4.507
IPC_peptide 8.799
IPC2_peptide 7.585
IPC2.peptide.svr19 7.69
Protein with the highest isoelectric point:
>tr|A0A385E5J9|A0A385E5J9_9GEMI Capsid protein OS=Geminiviridae sp. OX=2202566 PE=4 SV=1
MM1 pKa = 7.89 LEE3 pKa = 4.25 RR4 pKa = 11.84 CTLSPIRR11 pKa = 11.84 CASPVPWGITLRR23 pKa = 11.84 TLVGIVAVVRR33 pKa = 11.84 GTSARR38 pKa = 11.84 VSNPSMNFRR47 pKa = 11.84 SGAISRR53 pKa = 11.84 VRR55 pKa = 11.84 TLRR58 pKa = 11.84 ANEE61 pKa = 3.83 RR62 pKa = 11.84 TVVLRR67 pKa = 11.84 LRR69 pKa = 11.84 LGRR72 pKa = 11.84 LRR74 pKa = 11.84 RR75 pKa = 11.84 LLLILLNAIAPFLYY89 pKa = 10.57 VGLSLDD95 pKa = 4.86 LII97 pKa = 4.66
Molecular weight: 10.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.169
IPC2_protein 10.394
IPC_protein 11.901
Toseland 12.062
ProMoST 12.559
Dawson 12.062
Bjellqvist 12.062
Wikipedia 12.544
Rodwell 11.564
Grimsley 12.106
Solomon 12.559
Lehninger 12.457
Nozaki 12.062
DTASelect 12.062
Thurlkill 12.062
EMBOSS 12.559
Sillero 12.062
Patrickios 11.316
IPC_peptide 12.559
IPC2_peptide 11.55
IPC2.peptide.svr19 9.436
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
635
83
242
158.8
18.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.661 ± 0.458
1.732 ± 0.296
4.724 ± 0.846
4.252 ± 0.765
2.992 ± 0.259
5.669 ± 0.759
2.52 ± 0.631
5.827 ± 0.456
4.724 ± 1.75
10.551 ± 1.896
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.575 ± 0.217
3.937 ± 0.11
5.669 ± 0.507
3.465 ± 1.138
9.606 ± 2.109
6.142 ± 0.453
6.614 ± 0.91
5.669 ± 1.33
1.575 ± 0.573
4.094 ± 0.698
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here