Streptococcus satellite phage Javan387

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZLU0|A0A4D5ZLU0_9VIRU HTH cro/C1-type domain-containing protein OS=Streptococcus satellite phage Javan387 OX=2558671 GN=JavanS387_0003 PE=4 SV=1
MM1 pKa = 7.2KK2 pKa = 9.64TLSDD6 pKa = 3.59TPKK9 pKa = 9.76TFTFTTTLPNFEE21 pKa = 4.31QAQKK25 pKa = 10.83AGSALIGYY33 pKa = 7.54MIGTYY38 pKa = 7.57EE39 pKa = 4.35QSNIDD44 pKa = 2.91ITYY47 pKa = 8.42TGNSIQVDD55 pKa = 4.25YY56 pKa = 11.19ISDD59 pKa = 3.6EE60 pKa = 4.19DD61 pKa = 3.63LTEE64 pKa = 4.09SFEE67 pKa = 5.63RR68 pKa = 11.84ITNSLNHH75 pKa = 6.52TDD77 pKa = 4.41DD78 pKa = 4.35FEE80 pKa = 6.71DD81 pKa = 4.68YY82 pKa = 11.35EE83 pKa = 4.95DD84 pKa = 5.96FEE86 pKa = 7.21DD87 pKa = 6.31DD88 pKa = 5.15DD89 pKa = 4.06MTIKK93 pKa = 10.93GDD95 pKa = 3.73LDD97 pKa = 3.66NYY99 pKa = 8.62TALIGSFDD107 pKa = 3.9TLGEE111 pKa = 4.09AQAFTEE117 pKa = 4.58NLDD120 pKa = 4.44DD121 pKa = 6.72DD122 pKa = 4.55LTNGNNFIYY131 pKa = 8.79EE132 pKa = 4.09QCPNTVVLYY141 pKa = 8.63VSPQDD146 pKa = 3.9TIASSTIDD154 pKa = 3.69KK155 pKa = 10.75LIEE158 pKa = 4.29AYY160 pKa = 10.34VEE162 pKa = 4.2HH163 pKa = 6.72EE164 pKa = 4.52AGYY167 pKa = 10.81EE168 pKa = 3.73PDD170 pKa = 3.52TFRR173 pKa = 11.84LSYY176 pKa = 10.89HH177 pKa = 6.44DD178 pKa = 3.81MRR180 pKa = 11.84VSQLQSLDD188 pKa = 3.56EE189 pKa = 4.29DD190 pKa = 4.14TLINDD195 pKa = 4.09IIAYY199 pKa = 9.12EE200 pKa = 4.44LEE202 pKa = 4.52LLDD205 pKa = 4.44HH206 pKa = 7.54ADD208 pKa = 3.89RR209 pKa = 11.84LLSDD213 pKa = 4.22EE214 pKa = 5.14PLPLDD219 pKa = 4.17DD220 pKa = 3.37QHH222 pKa = 8.21GFEE225 pKa = 4.67TIEE228 pKa = 4.04LLGDD232 pKa = 3.73DD233 pKa = 4.96VIDD236 pKa = 4.37LVKK239 pKa = 10.76EE240 pKa = 3.72LDD242 pKa = 3.68TNKK245 pKa = 10.06EE246 pKa = 3.3FDD248 pKa = 4.71GIHH251 pKa = 7.27DD252 pKa = 4.33YY253 pKa = 11.0IIGGG257 pKa = 3.65

Molecular weight: 29.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZNB7|A0A4D5ZNB7_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan387 OX=2558671 GN=JavanS387_0020 PE=4 SV=1
MM1 pKa = 7.52SNYY4 pKa = 10.02KK5 pKa = 8.6VTQIYY10 pKa = 10.32RR11 pKa = 11.84DD12 pKa = 3.95DD13 pKa = 4.07KK14 pKa = 11.35STITKK19 pKa = 10.14NKK21 pKa = 10.2AEE23 pKa = 4.71AIEE26 pKa = 4.21KK27 pKa = 9.53KK28 pKa = 10.03KK29 pKa = 9.98KK30 pKa = 9.38HH31 pKa = 5.28EE32 pKa = 3.75RR33 pKa = 11.84VTRR36 pKa = 11.84FKK38 pKa = 10.85RR39 pKa = 11.84IRR41 pKa = 11.84KK42 pKa = 9.08RR43 pKa = 11.84GKK45 pKa = 9.37

Molecular weight: 5.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22

0

22

3213

44

524

146.0

16.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.855 ± 0.509

0.591 ± 0.148

6.785 ± 0.699

8.061 ± 0.909

3.61 ± 0.323

4.046 ± 0.357

1.65 ± 0.243

8.372 ± 0.479

10.115 ± 0.913

10.333 ± 0.518

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.521 ± 0.17

6.567 ± 0.492

3.019 ± 0.49

3.486 ± 0.361

4.015 ± 0.382

5.664 ± 0.511

6.411 ± 0.648

4.046 ± 0.375

0.622 ± 0.129

5.229 ± 0.369
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski