Amino acid dipepetide frequency for Cryobacterium sp. TmT2-48-2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.888AlaAla: 19.888 ± 0.222	0.684AlaCys: 0.684 ± 0.028	7.709AlaAsp: 7.709 ± 0.097	7.766AlaGlu: 7.766 ± 0.108	3.857AlaPhe: 3.857 ± 0.061	12.601AlaGly: 12.601 ± 0.138	2.29AlaHis: 2.29 ± 0.042	5.514AlaIle: 5.514 ± 0.078	2.691AlaLys: 2.691 ± 0.064	13.904AlaLeu: 13.904 ± 0.163	2.375AlaMet: 2.375 ± 0.057	2.574AlaAsn: 2.574 ± 0.058	6.299AlaPro: 6.299 ± 0.107	3.575AlaGln: 3.575 ± 0.058	8.593AlaArg: 8.593 ± 0.13	7.082AlaSer: 7.082 ± 0.089	7.565AlaThr: 7.565 ± 0.094	11.141AlaVal: 11.141 ± 0.127	1.727AlaTrp: 1.727 ± 0.044	2.197AlaTyr: 2.197 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	0.672CysAla: 0.672 ± 0.026	0.061CysCys: 0.061 ± 0.008	0.341CysAsp: 0.341 ± 0.022	0.273CysGlu: 0.273 ± 0.019	0.177CysPhe: 0.177 ± 0.015	0.594CysGly: 0.594 ± 0.028	0.126CysHis: 0.126 ± 0.011	0.192CysIle: 0.192 ± 0.014	0.096CysLys: 0.096 ± 0.011	0.516CysLeu: 0.516 ± 0.027	0.084CysMet: 0.084 ± 0.009	0.116CysAsn: 0.116 ± 0.013	0.307CysPro: 0.307 ± 0.017	0.126CysGln: 0.126 ± 0.012	0.373CysArg: 0.373 ± 0.021	0.374CysSer: 0.374 ± 0.021	0.382CysThr: 0.382 ± 0.021	0.475CysVal: 0.475 ± 0.027	0.084CysTrp: 0.084 ± 0.009	0.118CysTyr: 0.118 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.952AspAla: 7.952 ± 0.102	0.285AspCys: 0.285 ± 0.018	3.566AspAsp: 3.566 ± 0.079	3.66AspGlu: 3.66 ± 0.064	1.927AspPhe: 1.927 ± 0.05	5.281AspGly: 5.281 ± 0.077	1.144AspHis: 1.144 ± 0.035	2.422AspIle: 2.422 ± 0.052	1.097AspLys: 1.097 ± 0.036	6.388AspLeu: 6.388 ± 0.088	0.776AspMet: 0.776 ± 0.025	1.141AspAsn: 1.141 ± 0.037	3.954AspPro: 3.954 ± 0.07	1.832AspGln: 1.832 ± 0.041	4.324AspArg: 4.324 ± 0.098	2.996AspSer: 2.996 ± 0.061	3.289AspThr: 3.289 ± 0.072	4.901AspVal: 4.901 ± 0.075	0.913AspTrp: 0.913 ± 0.032	1.36AspTyr: 1.36 ± 0.035	0.002AspXaa: 0.002 ± 0.002
Glu	6.48GluAla: 6.48 ± 0.09	0.244GluCys: 0.244 ± 0.016	2.343GluAsp: 2.343 ± 0.054	2.651GluGlu: 2.651 ± 0.061	1.782GluPhe: 1.782 ± 0.051	3.501GluGly: 3.501 ± 0.067	1.278GluHis: 1.278 ± 0.036	2.767GluIle: 2.767 ± 0.065	1.512GluLys: 1.512 ± 0.048	6.367GluLeu: 6.367 ± 0.087	0.997GluMet: 0.997 ± 0.031	1.337GluAsn: 1.337 ± 0.045	2.902GluPro: 2.902 ± 0.057	2.057GluGln: 2.057 ± 0.049	4.326GluArg: 4.326 ± 0.082	3.015GluSer: 3.015 ± 0.056	3.186GluThr: 3.186 ± 0.06	4.235GluVal: 4.235 ± 0.074	0.764GluTrp: 0.764 ± 0.028	1.079GluTyr: 1.079 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	4.042PheAla: 4.042 ± 0.08	0.201PheCys: 0.201 ± 0.016	2.368PheAsp: 2.368 ± 0.048	1.777PheGlu: 1.777 ± 0.045	1.153PhePhe: 1.153 ± 0.04	3.588PheGly: 3.588 ± 0.068	0.586PheHis: 0.586 ± 0.024	1.351PheIle: 1.351 ± 0.044	0.601PheLys: 0.601 ± 0.026	3.125PheLeu: 3.125 ± 0.074	0.491PheMet: 0.491 ± 0.025	0.788PheAsn: 0.788 ± 0.031	1.514PhePro: 1.514 ± 0.04	0.851PheGln: 0.851 ± 0.03	1.803PheArg: 1.803 ± 0.048	1.974PheSer: 1.974 ± 0.051	2.255PheThr: 2.255 ± 0.05	2.914PheVal: 2.914 ± 0.058	0.497PheTrp: 0.497 ± 0.024	0.744PheTyr: 0.744 ± 0.033	0.0PheXaa: 0.0 ± 0.0
Gly	10.224GlyAla: 10.224 ± 0.128	0.619GlyCys: 0.619 ± 0.029	4.779GlyAsp: 4.779 ± 0.078	4.469GlyGlu: 4.469 ± 0.077	3.414GlyPhe: 3.414 ± 0.068	7.164GlyGly: 7.164 ± 0.107	1.866GlyHis: 1.866 ± 0.045	4.906GlyIle: 4.906 ± 0.069	2.293GlyLys: 2.293 ± 0.051	9.532GlyLeu: 9.532 ± 0.108	1.905GlyMet: 1.905 ± 0.05	1.931GlyAsn: 1.931 ± 0.051	3.937GlyPro: 3.937 ± 0.075	2.788GlyGln: 2.788 ± 0.064	6.16GlyArg: 6.16 ± 0.087	5.646GlySer: 5.646 ± 0.081	6.092GlyThr: 6.092 ± 0.092	7.591GlyVal: 7.591 ± 0.089	1.546GlyTrp: 1.546 ± 0.049	2.217GlyTyr: 2.217 ± 0.06	0.0GlyXaa: 0.0 ± 0.0
His	2.209HisAla: 2.209 ± 0.053	0.138HisCys: 0.138 ± 0.012	1.212HisAsp: 1.212 ± 0.036	1.039HisGlu: 1.039 ± 0.034	0.539HisPhe: 0.539 ± 0.025	1.905HisGly: 1.905 ± 0.049	0.564HisHis: 0.564 ± 0.028	0.744HisIle: 0.744 ± 0.029	0.337HisLys: 0.337 ± 0.02	2.144HisLeu: 2.144 ± 0.052	0.278HisMet: 0.278 ± 0.019	0.422HisAsn: 0.422 ± 0.022	1.53HisPro: 1.53 ± 0.036	0.55HisGln: 0.55 ± 0.025	1.543HisArg: 1.543 ± 0.044	1.127HisSer: 1.127 ± 0.034	1.084HisThr: 1.084 ± 0.036	1.45HisVal: 1.45 ± 0.049	0.286HisTrp: 0.286 ± 0.015	0.45HisTyr: 0.45 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	6.281IleAla: 6.281 ± 0.089	0.266IleCys: 0.266 ± 0.017	3.498IleAsp: 3.498 ± 0.059	2.805IleGlu: 2.805 ± 0.062	1.367IlePhe: 1.367 ± 0.043	4.609IleGly: 4.609 ± 0.086	0.726IleHis: 0.726 ± 0.03	2.062IleIle: 2.062 ± 0.049	0.95IleLys: 0.95 ± 0.038	4.157IleLeu: 4.157 ± 0.079	0.72IleMet: 0.72 ± 0.033	1.091IleAsn: 1.091 ± 0.037	2.462IlePro: 2.462 ± 0.053	1.117IleGln: 1.117 ± 0.035	3.032IleArg: 3.032 ± 0.055	2.54IleSer: 2.54 ± 0.052	2.961IleThr: 2.961 ± 0.065	4.71IleVal: 4.71 ± 0.079	0.521IleTrp: 0.521 ± 0.024	0.816IleTyr: 0.816 ± 0.028	0.0IleXaa: 0.0 ± 0.0
Lys	2.626LysAla: 2.626 ± 0.063	0.085LysCys: 0.085 ± 0.011	1.132LysAsp: 1.132 ± 0.042	0.99LysGlu: 0.99 ± 0.038	0.622LysPhe: 0.622 ± 0.029	1.586LysGly: 1.586 ± 0.049	0.456LysHis: 0.456 ± 0.019	1.117LysIle: 1.117 ± 0.039	0.909LysLys: 0.909 ± 0.042	2.155LysLeu: 2.155 ± 0.047	0.531LysMet: 0.531 ± 0.024	0.672LysAsn: 0.672 ± 0.03	1.383LysPro: 1.383 ± 0.038	0.776LysGln: 0.776 ± 0.029	1.665LysArg: 1.665 ± 0.047	1.414LysSer: 1.414 ± 0.041	1.498LysThr: 1.498 ± 0.045	1.785LysVal: 1.785 ± 0.052	0.281LysTrp: 0.281 ± 0.017	0.492LysTyr: 0.492 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	15.059LeuAla: 15.059 ± 0.179	0.575LeuCys: 0.575 ± 0.029	6.473LeuAsp: 6.473 ± 0.091	5.029LeuGlu: 5.029 ± 0.079	3.111LeuPhe: 3.111 ± 0.07	9.769LeuGly: 9.769 ± 0.105	1.984LeuHis: 1.984 ± 0.047	4.965LeuIle: 4.965 ± 0.082	2.113LeuLys: 2.113 ± 0.05	11.272LeuLeu: 11.272 ± 0.148	1.779LeuMet: 1.779 ± 0.041	2.315LeuAsn: 2.315 ± 0.057	5.722LeuPro: 5.722 ± 0.093	2.579LeuGln: 2.579 ± 0.047	7.242LeuArg: 7.242 ± 0.108	6.246LeuSer: 6.246 ± 0.097	6.952LeuThr: 6.952 ± 0.089	9.629LeuVal: 9.629 ± 0.123	1.222LeuTrp: 1.222 ± 0.041	1.724LeuTyr: 1.724 ± 0.043	0.0LeuXaa: 0.0 ± 0.0
Met	2.182MetAla: 2.182 ± 0.046	0.09MetCys: 0.09 ± 0.011	0.773MetAsp: 0.773 ± 0.029	0.706MetGlu: 0.706 ± 0.027	0.578MetPhe: 0.578 ± 0.028	1.316MetGly: 1.316 ± 0.044	0.333MetHis: 0.333 ± 0.017	0.949MetIle: 0.949 ± 0.034	0.535MetLys: 0.535 ± 0.024	1.945MetLeu: 1.945 ± 0.047	0.327MetMet: 0.327 ± 0.019	0.54MetAsn: 0.54 ± 0.022	1.091MetPro: 1.091 ± 0.035	0.537MetGln: 0.537 ± 0.029	1.25MetArg: 1.25 ± 0.038	1.417MetSer: 1.417 ± 0.043	1.652MetThr: 1.652 ± 0.045	1.472MetVal: 1.472 ± 0.04	0.177MetTrp: 0.177 ± 0.013	0.251MetTyr: 0.251 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	2.693AsnAla: 2.693 ± 0.057	0.146AsnCys: 0.146 ± 0.012	1.267AsnAsp: 1.267 ± 0.042	1.135AsnGlu: 1.135 ± 0.039	0.75AsnPhe: 0.75 ± 0.032	2.142AsnGly: 2.142 ± 0.049	0.418AsnHis: 0.418 ± 0.022	0.938AsnIle: 0.938 ± 0.035	0.479AsnLys: 0.479 ± 0.026	2.381AsnLeu: 2.381 ± 0.052	0.366AsnMet: 0.366 ± 0.021	0.566AsnAsn: 0.566 ± 0.029	1.779AsnPro: 1.779 ± 0.051	0.715AsnGln: 0.715 ± 0.026	1.525AsnArg: 1.525 ± 0.04	1.198AsnSer: 1.198 ± 0.04	1.414AsnThr: 1.414 ± 0.048	1.812AsnVal: 1.812 ± 0.045	0.346AsnTrp: 0.346 ± 0.02	0.552AsnTyr: 0.552 ± 0.027	0.0AsnXaa: 0.0 ± 0.0
Pro	7.559ProAla: 7.559 ± 0.122	0.219ProCys: 0.219 ± 0.014	3.804ProAsp: 3.804 ± 0.061	3.601ProGlu: 3.601 ± 0.06	1.678ProPhe: 1.678 ± 0.051	5.33ProGly: 5.33 ± 0.084	1.068ProHis: 1.068 ± 0.035	2.217ProIle: 2.217 ± 0.046	1.105ProLys: 1.105 ± 0.037	5.003ProLeu: 5.003 ± 0.079	0.947ProMet: 0.947 ± 0.032	1.243ProAsn: 1.243 ± 0.036	2.352ProPro: 2.352 ± 0.059	1.388ProGln: 1.388 ± 0.035	3.28ProArg: 3.28 ± 0.062	3.068ProSer: 3.068 ± 0.062	3.52ProThr: 3.52 ± 0.071	5.034ProVal: 5.034 ± 0.084	0.831ProTrp: 0.831 ± 0.028	0.984ProTyr: 0.984 ± 0.036	0.001ProXaa: 0.001 ± 0.001
Gln	3.834GlnAla: 3.834 ± 0.077	0.141GlnCys: 0.141 ± 0.013	1.314GlnAsp: 1.314 ± 0.039	1.211GlnGlu: 1.211 ± 0.034	0.862GlnPhe: 0.862 ± 0.027	2.192GlnGly: 2.192 ± 0.045	0.613GlnHis: 0.613 ± 0.026	1.613GlnIle: 1.613 ± 0.041	0.805GlnLys: 0.805 ± 0.031	3.189GlnLeu: 3.189 ± 0.059	0.537GlnMet: 0.537 ± 0.022	0.763GlnAsn: 0.763 ± 0.03	1.465GlnPro: 1.465 ± 0.042	1.073GlnGln: 1.073 ± 0.039	2.158GlnArg: 2.158 ± 0.055	1.739GlnSer: 1.739 ± 0.04	1.669GlnThr: 1.669 ± 0.044	2.635GlnVal: 2.635 ± 0.062	0.399GlnTrp: 0.399 ± 0.02	0.554GlnTyr: 0.554 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	8.225ArgAla: 8.225 ± 0.121	0.344ArgCys: 0.344 ± 0.02	3.889ArgAsp: 3.889 ± 0.073	3.701ArgGlu: 3.701 ± 0.075	2.459ArgPhe: 2.459 ± 0.055	5.135ArgGly: 5.135 ± 0.079	1.505ArgHis: 1.505 ± 0.041	3.381ArgIle: 3.381 ± 0.061	1.586ArgLys: 1.586 ± 0.05	7.56ArgLeu: 7.56 ± 0.102	1.695ArgMet: 1.695 ± 0.042	1.523ArgAsn: 1.523 ± 0.041	3.621ArgPro: 3.621 ± 0.073	2.198ArgGln: 2.198 ± 0.046	5.877ArgArg: 5.877 ± 0.124	4.284ArgSer: 4.284 ± 0.077	4.108ArgThr: 4.108 ± 0.066	5.665ArgVal: 5.665 ± 0.09	1.049ArgTrp: 1.049 ± 0.034	1.548ArgTyr: 1.548 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	7.204SerAla: 7.204 ± 0.095	0.319SerCys: 0.319 ± 0.02	3.168SerAsp: 3.168 ± 0.054	2.739SerGlu: 2.739 ± 0.054	1.983SerPhe: 1.983 ± 0.047	5.92SerGly: 5.92 ± 0.093	1.057SerHis: 1.057 ± 0.034	2.753SerIle: 2.753 ± 0.059	1.219SerLys: 1.219 ± 0.039	5.87SerLeu: 5.87 ± 0.075	1.165SerMet: 1.165 ± 0.038	1.275SerAsn: 1.275 ± 0.035	3.312SerPro: 3.312 ± 0.061	1.535SerGln: 1.535 ± 0.037	3.877SerArg: 3.877 ± 0.063	3.477SerSer: 3.477 ± 0.071	3.929SerThr: 3.929 ± 0.07	5.413SerVal: 5.413 ± 0.089	0.846SerTrp: 0.846 ± 0.03	1.263SerTyr: 1.263 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	7.975ThrAla: 7.975 ± 0.096	0.308ThrCys: 0.308 ± 0.019	4.16ThrAsp: 4.16 ± 0.062	3.375ThrGlu: 3.375 ± 0.063	1.982ThrPhe: 1.982 ± 0.051	6.486ThrGly: 6.486 ± 0.091	1.162ThrHis: 1.162 ± 0.039	3.004ThrIle: 3.004 ± 0.055	1.28ThrLys: 1.28 ± 0.045	6.527ThrLeu: 6.527 ± 0.089	1.062ThrMet: 1.062 ± 0.031	1.395ThrAsn: 1.395 ± 0.045	4.042ThrPro: 4.042 ± 0.077	1.534ThrGln: 1.534 ± 0.043	3.95ThrArg: 3.95 ± 0.068	3.419ThrSer: 3.419 ± 0.069	3.809ThrThr: 3.809 ± 0.08	6.234ThrVal: 6.234 ± 0.086	0.788ThrTrp: 0.788 ± 0.036	1.092ThrTyr: 1.092 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	11.092ValAla: 11.092 ± 0.126	0.534ValCys: 0.534 ± 0.028	5.467ValAsp: 5.467 ± 0.084	4.336ValGlu: 4.336 ± 0.078	3.088ValPhe: 3.088 ± 0.055	7.163ValGly: 7.163 ± 0.096	1.734ValHis: 1.734 ± 0.039	4.414ValIle: 4.414 ± 0.079	1.838ValLys: 1.838 ± 0.048	9.585ValLeu: 9.585 ± 0.116	1.52ValMet: 1.52 ± 0.043	2.003ValAsn: 2.003 ± 0.048	4.771ValPro: 4.771 ± 0.084	2.355ValGln: 2.355 ± 0.052	5.71ValArg: 5.71 ± 0.085	5.206ValSer: 5.206 ± 0.079	6.186ValThr: 6.186 ± 0.087	8.16ValVal: 8.16 ± 0.122	1.054ValTrp: 1.054 ± 0.035	1.592ValTyr: 1.592 ± 0.042	0.0ValXaa: 0.0 ± 0.0
Trp	1.561TrpAla: 1.561 ± 0.044	0.098TrpCys: 0.098 ± 0.009	0.707TrpAsp: 0.707 ± 0.03	0.511TrpGlu: 0.511 ± 0.025	0.553TrpPhe: 0.553 ± 0.027	0.931TrpGly: 0.931 ± 0.034	0.318TrpHis: 0.318 ± 0.019	0.657TrpIle: 0.657 ± 0.025	0.32TrpLys: 0.32 ± 0.023	1.8TrpLeu: 1.8 ± 0.048	0.318TrpMet: 0.318 ± 0.021	0.419TrpAsn: 0.419 ± 0.023	0.719TrpPro: 0.719 ± 0.029	0.592TrpGln: 0.592 ± 0.027	1.02TrpArg: 1.02 ± 0.035	0.863TrpSer: 0.863 ± 0.033	0.889TrpThr: 0.889 ± 0.03	1.073TrpVal: 1.073 ± 0.033	0.307TrpTrp: 0.307 ± 0.019	0.296TrpTyr: 0.296 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.275TyrAla: 2.275 ± 0.054	0.129TyrCys: 0.129 ± 0.011	1.242TyrAsp: 1.242 ± 0.041	1.048TyrGlu: 1.048 ± 0.035	0.741TyrPhe: 0.741 ± 0.026	1.869TyrGly: 1.869 ± 0.045	0.324TyrHis: 0.324 ± 0.021	0.725TyrIle: 0.725 ± 0.031	0.409TyrLys: 0.409 ± 0.02	2.337TyrLeu: 2.337 ± 0.05	0.247TyrMet: 0.247 ± 0.017	0.505TyrAsn: 0.505 ± 0.026	1.071TyrPro: 1.071 ± 0.034	0.589TyrGln: 0.589 ± 0.026	1.645TyrArg: 1.645 ± 0.046	1.236TyrSer: 1.236 ± 0.041	1.143TyrThr: 1.143 ± 0.035	1.48TyrVal: 1.48 ± 0.037	0.313TyrTrp: 0.313 ± 0.02	0.478TyrTyr: 0.478 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.001	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2868 proteins (931452 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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