Thermobifida fusca (strain YX)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Nocardiopsaceae; Thermobifida; Thermobifida fusca

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3085 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q47LA5|Q47LA5_THEFY Redox-sensing transcriptional repressor Rex OS=Thermobifida fusca (strain YX) OX=269800 GN=rex PE=3 SV=1
MM1 pKa = 7.59KK2 pKa = 10.35LIRR5 pKa = 11.84YY6 pKa = 7.58GKK8 pKa = 9.43IAGVTLASALALSACGSDD26 pKa = 3.27QAVAPEE32 pKa = 4.3EE33 pKa = 4.33TGGTAAGSVDD43 pKa = 3.92CVQGGGTLAGAGASSQEE60 pKa = 3.92KK61 pKa = 10.7AMAAWVAAYY70 pKa = 8.57TSACADD76 pKa = 3.34TTVNYY81 pKa = 10.7DD82 pKa = 3.4SVGSGAGRR90 pKa = 11.84SQFIEE95 pKa = 4.34GAVSFAGSDD104 pKa = 3.31SPMDD108 pKa = 3.74EE109 pKa = 4.7EE110 pKa = 4.37EE111 pKa = 4.12TAQATEE117 pKa = 3.93RR118 pKa = 11.84CGGSEE123 pKa = 4.0AVHH126 pKa = 6.15LPAYY130 pKa = 9.26ISPIAIIFNLEE141 pKa = 4.02GVDD144 pKa = 3.87SLNLRR149 pKa = 11.84PEE151 pKa = 4.24VIADD155 pKa = 3.81IFNQKK160 pKa = 6.67ITKK163 pKa = 9.23WNDD166 pKa = 2.63EE167 pKa = 4.76AIAADD172 pKa = 4.24NPDD175 pKa = 3.4VDD177 pKa = 5.8LPDD180 pKa = 5.2ADD182 pKa = 4.28IIPVNRR188 pKa = 11.84SDD190 pKa = 4.43EE191 pKa = 4.41SGTTEE196 pKa = 4.66NFVQYY201 pKa = 10.4LSEE204 pKa = 4.38AAPDD208 pKa = 3.54HH209 pKa = 6.52WPHH212 pKa = 6.05EE213 pKa = 4.53VSGDD217 pKa = 3.32WPIPPVEE224 pKa = 4.14AAQGSSGVVSAVQGGTGTIGYY245 pKa = 9.55VDD247 pKa = 4.12ASHH250 pKa = 6.2ATEE253 pKa = 4.59FGTVAVGVGDD263 pKa = 3.74SFVPYY268 pKa = 10.26SPEE271 pKa = 3.3AAAAIVDD278 pKa = 3.9SAEE281 pKa = 3.81PRR283 pKa = 11.84EE284 pKa = 4.38GNTEE288 pKa = 3.6NDD290 pKa = 3.48LAVSLNYY297 pKa = 9.47ATDD300 pKa = 3.37EE301 pKa = 4.66DD302 pKa = 4.48GVYY305 pKa = 10.51PIVLVSYY312 pKa = 9.38EE313 pKa = 4.32IVCLEE318 pKa = 4.53YY319 pKa = 10.73EE320 pKa = 4.5DD321 pKa = 3.69QTEE324 pKa = 3.98ADD326 pKa = 4.0LVKK329 pKa = 10.96SFIGYY334 pKa = 9.31VISEE338 pKa = 4.47EE339 pKa = 4.35GQQAAANEE347 pKa = 4.47AGSAPLSDD355 pKa = 3.34ATRR358 pKa = 11.84DD359 pKa = 3.58KK360 pKa = 10.99IQAVLDD366 pKa = 4.4KK367 pKa = 10.82IQVASS372 pKa = 3.64

Molecular weight: 38.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q47LK0|RL16_THEFY 50S ribosomal protein L16 OS=Thermobifida fusca (strain YX) OX=269800 GN=rplP PE=3 SV=1
MM1 pKa = 7.28GSVVKK6 pKa = 10.49KK7 pKa = 9.45RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.48RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.55LLKK22 pKa = 7.77KK23 pKa = 9.13TRR25 pKa = 11.84IARR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.8KK32 pKa = 9.86

Molecular weight: 3.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3085

0

3085

1025469

32

3629

332.4

35.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.602 ± 0.061

0.803 ± 0.011

5.816 ± 0.039

6.348 ± 0.042

2.827 ± 0.022

8.379 ± 0.042

2.321 ± 0.019

3.881 ± 0.029

1.997 ± 0.032

10.412 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.747 ± 0.017

1.953 ± 0.024

6.117 ± 0.043

3.004 ± 0.028

8.2 ± 0.047

5.215 ± 0.033

6.001 ± 0.029

8.693 ± 0.046

1.496 ± 0.018

2.185 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski