Wuhan nido-like virus 1
Average proteome isoelectric point is 7.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KJ46|A0A1L3KJ46_9VIRU 1ab OS=Wuhan nido-like virus 1 OX=1923743 PE=4 SV=1
MM1 pKa = 7.69 ISMRR5 pKa = 11.84 AVCGILALLAISGVLSHH22 pKa = 6.44 TVVNYY27 pKa = 8.88 NHH29 pKa = 6.59 EE30 pKa = 5.08 LIPEE34 pKa = 4.68 DD35 pKa = 5.0 FQFTDD40 pKa = 3.7 CPDD43 pKa = 4.13 RR44 pKa = 11.84 IIIRR48 pKa = 11.84 DD49 pKa = 3.66 GLNKK53 pKa = 10.15 YY54 pKa = 9.63 NEE56 pKa = 4.1 NVTVYY61 pKa = 10.05 FLRR64 pKa = 11.84 DD65 pKa = 3.56 KK66 pKa = 11.09 NGKK69 pKa = 8.92 DD70 pKa = 3.0 ANKK73 pKa = 10.37 RR74 pKa = 11.84 LDD76 pKa = 3.45 QLGVGVSLHH85 pKa = 6.56 GYY87 pKa = 6.94 WPKK90 pKa = 10.16 PRR92 pKa = 11.84 CYY94 pKa = 10.54 NDD96 pKa = 2.95 NGVIKK101 pKa = 10.12 SVKK104 pKa = 10.18 SFMEE108 pKa = 3.87 RR109 pKa = 11.84 QVISQSWDD117 pKa = 2.95 LKK119 pKa = 10.8 KK120 pKa = 10.59 EE121 pKa = 3.89 YY122 pKa = 11.06 NEE124 pKa = 3.47 IRR126 pKa = 11.84 KK127 pKa = 9.11 FIIDD131 pKa = 3.3 KK132 pKa = 10.33 DD133 pKa = 4.16 YY134 pKa = 9.14 RR135 pKa = 11.84 DD136 pKa = 3.13 KK137 pKa = 10.85 WISHH141 pKa = 4.86 TSKK144 pKa = 10.04 WDD146 pKa = 2.96 QWYY149 pKa = 8.98 NNEE152 pKa = 4.1 YY153 pKa = 10.17 KK154 pKa = 10.54 KK155 pKa = 10.26 HH156 pKa = 5.56 FFNEE160 pKa = 4.89 GYY162 pKa = 10.75 NLDD165 pKa = 3.83 KK166 pKa = 11.13 FFMKK170 pKa = 10.68 VEE172 pKa = 4.03 DD173 pKa = 4.19 SIEE176 pKa = 3.95 NLKK179 pKa = 10.34 IKK181 pKa = 9.94 PLSPHH186 pKa = 5.89 EE187 pKa = 4.29 CMFSKK192 pKa = 10.46 DD193 pKa = 3.28 KK194 pKa = 10.81 KK195 pKa = 9.85 INRR198 pKa = 11.84 VTQLVTSEE206 pKa = 4.27 TPEE209 pKa = 4.0 KK210 pKa = 10.67 NSCKK214 pKa = 9.92 KK215 pKa = 10.55 DD216 pKa = 3.53 FEE218 pKa = 4.53 YY219 pKa = 10.78 KK220 pKa = 10.25 IINEE224 pKa = 4.06 NQVVKK229 pKa = 10.83 EE230 pKa = 4.27 DD231 pKa = 3.98 GFPVHH236 pKa = 7.32 IDD238 pKa = 3.43 EE239 pKa = 5.54 NGKK242 pKa = 10.05 LMQKK246 pKa = 10.52 DD247 pKa = 3.47 PTGNPSLYY255 pKa = 10.59 DD256 pKa = 3.3 QIEE259 pKa = 3.94 QFFTHH264 pKa = 6.91 FEE266 pKa = 3.71 MDD268 pKa = 2.97 MEE270 pKa = 4.89 YY271 pKa = 8.84 ITDD274 pKa = 5.44 GITSIKK280 pKa = 10.68 NYY282 pKa = 10.24 LSEE285 pKa = 5.5 FKK287 pKa = 11.0 GFWHH291 pKa = 6.97 ILATGFWSMSLTAWQLFGKK310 pKa = 9.04 VKK312 pKa = 9.7 KK313 pKa = 10.75 GKK315 pKa = 9.84 IIYY318 pKa = 9.19 IHH320 pKa = 5.9 EE321 pKa = 4.45 FEE323 pKa = 5.23 LFEE326 pKa = 4.24 TYY328 pKa = 10.66 YY329 pKa = 10.89 QFILHH334 pKa = 6.28 KK335 pKa = 9.93 HH336 pKa = 6.04 HH337 pKa = 6.56 IVVFGFGVTLSLFLASMLDD356 pKa = 3.99 SATLWIISIVLNVGFFINYY375 pKa = 8.81 FFGLNKK381 pKa = 10.1 KK382 pKa = 7.46 EE383 pKa = 4.18 NKK385 pKa = 9.75 GKK387 pKa = 10.47 LFIAGAVVMLVYY399 pKa = 11.23 SMGCYY404 pKa = 8.82 STTTYY409 pKa = 10.93 YY410 pKa = 10.92 EE411 pKa = 3.92 NGFKK415 pKa = 10.38 RR416 pKa = 11.84 VNDD419 pKa = 3.33 VDD421 pKa = 3.58 ITQVPASDD429 pKa = 4.68 RR430 pKa = 11.84 IDD432 pKa = 3.83 LEE434 pKa = 4.51 MYY436 pKa = 10.88 LSDD439 pKa = 3.59 NVYY442 pKa = 10.56 KK443 pKa = 11.05 AFGGYY448 pKa = 10.36 YY449 pKa = 9.33 NLSLIYY455 pKa = 9.23 VTIICMVMLVNMVRR469 pKa = 11.84 LFKK472 pKa = 10.38 VWKK475 pKa = 9.53 HH476 pKa = 4.62 LTDD479 pKa = 3.31 VEE481 pKa = 4.81 SFTKK485 pKa = 10.53 EE486 pKa = 3.74 SAQKK490 pKa = 10.43 KK491 pKa = 9.19 IVVAINGFTFALTTMMLSYY510 pKa = 10.39 IYY512 pKa = 10.95 LNMTRR517 pKa = 11.84 VITIDD522 pKa = 2.96 NSLIINQDD530 pKa = 3.13 VAHH533 pKa = 6.78 SKK535 pKa = 8.93 TKK537 pKa = 10.42 YY538 pKa = 9.16 IAIIMLILTALNEE551 pKa = 3.96 IMAFMPYY558 pKa = 10.13 AQAPINLMMTISVLVMCVLKK578 pKa = 10.78 KK579 pKa = 10.77 SLITEE584 pKa = 4.59 FVDD587 pKa = 2.81 ILYY590 pKa = 10.46 RR591 pKa = 11.84 GFLMYY596 pKa = 10.07 YY597 pKa = 9.87 HH598 pKa = 7.04 SPTGSSLLIFAASLVALIMFKK619 pKa = 9.57 YY620 pKa = 9.19 TYY622 pKa = 10.37 RR623 pKa = 11.84 HH624 pKa = 5.13 FNMWLLMLEE633 pKa = 5.25 FITLVPFVKK642 pKa = 10.41 FVNRR646 pKa = 11.84 LPIINLEE653 pKa = 4.19 KK654 pKa = 10.58 NVKK657 pKa = 9.89 NLLEE661 pKa = 4.26 RR662 pKa = 11.84 MGLEE666 pKa = 4.35 DD667 pKa = 5.21 LLPVYY672 pKa = 10.05 IVLMVLYY679 pKa = 11.05 VMLKK683 pKa = 10.42 LNGKK687 pKa = 8.35 IVRR690 pKa = 11.84 TPKK693 pKa = 10.18 QYY695 pKa = 11.39 YY696 pKa = 9.44 KK697 pKa = 10.82 VINGKK702 pKa = 10.29 LIMHH706 pKa = 7.01 GNLGKK711 pKa = 10.42 CIKK714 pKa = 10.6 NITLVFMEE722 pKa = 4.45 ILVVLAMISWALGTVYY738 pKa = 10.9 GLVRR742 pKa = 11.84 QYY744 pKa = 11.72 LDD746 pKa = 3.58 NNTEE750 pKa = 4.59 NNASWYY756 pKa = 8.83 VHH758 pKa = 4.97 MAFGIFCLWQIGVKK772 pKa = 9.79 ILLINSIRR780 pKa = 11.84 KK781 pKa = 9.17 VEE783 pKa = 4.19 TEE785 pKa = 3.96 TSKK788 pKa = 8.07 TTMISMIVYY797 pKa = 9.53 AAVFGLFMIYY807 pKa = 10.31 VGLQYY812 pKa = 10.46 LVWMIRR818 pKa = 11.84 YY819 pKa = 8.16 DD820 pKa = 3.42 QYY822 pKa = 11.16 INGIIGVFFNGMPYY836 pKa = 10.06 IFGTMIVEE844 pKa = 4.67 IIRR847 pKa = 11.84 RR848 pKa = 11.84 LIAPIFCLMRR858 pKa = 11.84 EE859 pKa = 4.11 RR860 pKa = 11.84 FYY862 pKa = 10.83 NTHH865 pKa = 7.57 DD866 pKa = 3.24 EE867 pKa = 4.46 FTRR870 pKa = 11.84 NMLSKK875 pKa = 11.18 AEE877 pKa = 4.5 TLNQWITNSCLMVKK891 pKa = 10.52 LSISIIYY898 pKa = 9.82 ALSAMVKK905 pKa = 8.57 TGWIAMIYY913 pKa = 9.13 NHH915 pKa = 6.37 DD916 pKa = 4.71 HH917 pKa = 6.83 IEE919 pKa = 4.62 TIASNYY925 pKa = 9.39 EE926 pKa = 3.75 VFQTVIVLCATFAALFEE943 pKa = 4.07 FDD945 pKa = 4.57 KK946 pKa = 11.24 IIVEE950 pKa = 4.22 WFLGEE955 pKa = 3.94 IQVKK959 pKa = 10.12 EE960 pKa = 3.87 KK961 pKa = 10.02 LTYY964 pKa = 10.49 EE965 pKa = 3.82 RR966 pKa = 11.84 VKK968 pKa = 11.41 YY969 pKa = 11.0 NDD971 pKa = 3.07 IKK973 pKa = 11.08 RR974 pKa = 11.84 NAIFTDD980 pKa = 3.96 DD981 pKa = 3.72 LWQEE985 pKa = 4.09 LTKK988 pKa = 10.83 DD989 pKa = 3.5 DD990 pKa = 3.71 QDD992 pKa = 3.26 RR993 pKa = 11.84 VEE995 pKa = 3.97 MSKK998 pKa = 11.09 NKK1000 pKa = 9.44 TNLGEE1005 pKa = 4.33 VKK1007 pKa = 9.9 TGTKK1011 pKa = 9.96 RR1012 pKa = 11.84 NYY1014 pKa = 10.54 DD1015 pKa = 3.29 NARR1018 pKa = 11.84 RR1019 pKa = 11.84 LLNEE1023 pKa = 4.2 TEE1025 pKa = 4.29 SDD1027 pKa = 3.48 LTTTKK1032 pKa = 8.8 QTEE1035 pKa = 4.4 VFTEE1039 pKa = 3.76 VLPAVNEE1046 pKa = 3.93 RR1047 pKa = 11.84 DD1048 pKa = 3.31 MSKK1051 pKa = 10.01 IIHH1054 pKa = 6.32 KK1055 pKa = 10.56 SKK1057 pKa = 10.61 FVLKK1061 pKa = 9.51 EE1062 pKa = 3.71 KK1063 pKa = 10.56 KK1064 pKa = 10.11 YY1065 pKa = 10.49 VIQNEE1070 pKa = 4.19 QCKK1073 pKa = 10.37 LDD1075 pKa = 4.98 DD1076 pKa = 3.8 EE1077 pKa = 5.37 LEE1079 pKa = 4.33 INFEE1083 pKa = 4.71 DD1084 pKa = 4.76 YY1085 pKa = 11.25 SKK1087 pKa = 11.56 NPNTYY1092 pKa = 9.22 IQNEE1096 pKa = 4.36 DD1097 pKa = 4.31 YY1098 pKa = 10.53 IPLMMEE1104 pKa = 3.85 IDD1106 pKa = 3.86 SKK1108 pKa = 11.17 IKK1110 pKa = 9.95 EE1111 pKa = 4.46 KK1112 pKa = 9.43 KK1113 pKa = 7.97 TAMGLQSVGQKK1124 pKa = 10.08 NAVHH1128 pKa = 6.26 MEE1130 pKa = 4.34 MISKK1134 pKa = 10.32 AGQNLQKK1141 pKa = 10.79 LCDD1144 pKa = 3.76 IYY1146 pKa = 11.58 SPLAGYY1152 pKa = 10.72 GRR1154 pKa = 11.84 FSVVQEE1160 pKa = 4.28 TNWRR1164 pKa = 11.84 FVNFLIPYY1172 pKa = 8.16 MSLSSFFLEE1181 pKa = 4.72 VIHH1184 pKa = 6.63 GIWGGMDD1191 pKa = 3.33 KK1192 pKa = 10.95 LAGLLSVDD1200 pKa = 3.75 LTKK1203 pKa = 10.8 TPIILAAKK1211 pKa = 9.92 GNAKK1215 pKa = 10.56 DD1216 pKa = 3.91 EE1217 pKa = 4.33 ITNKK1221 pKa = 10.59 DD1222 pKa = 2.7 IFTIEE1227 pKa = 3.91 EE1228 pKa = 4.19 YY1229 pKa = 10.91 FRR1231 pKa = 11.84 ITGKK1235 pKa = 8.57 TYY1237 pKa = 10.78 KK1238 pKa = 10.29 DD1239 pKa = 3.37 LRR1241 pKa = 11.84 DD1242 pKa = 4.05 HH1243 pKa = 7.44 IKK1245 pKa = 9.12 NTDD1248 pKa = 3.44 EE1249 pKa = 4.32 KK1250 pKa = 10.86 HH1251 pKa = 7.26 DD1252 pKa = 4.07 NLIPNSDD1259 pKa = 3.85 TIYY1262 pKa = 11.02 VVGSSKK1268 pKa = 11.05 GKK1270 pKa = 10.28 LFVAFDD1276 pKa = 4.39 LNIVTQLEE1284 pKa = 3.97 QRR1286 pKa = 11.84 EE1287 pKa = 4.21 NSSIMGEE1294 pKa = 3.99 NIFGVFHH1301 pKa = 6.58 YY1302 pKa = 11.13 YY1303 pKa = 10.07 EE1304 pKa = 4.83 LEE1306 pKa = 3.81 QARR1309 pKa = 11.84 TVRR1312 pKa = 11.84 PSRR1315 pKa = 11.84 IEE1317 pKa = 3.7 NDD1319 pKa = 3.18 MTLSTSGTFGGLRR1332 pKa = 11.84 YY1333 pKa = 9.54 SVPKK1337 pKa = 9.77 GTGLLYY1343 pKa = 10.96 CNSLNMYY1350 pKa = 10.12 LHH1352 pKa = 5.72 EE1353 pKa = 5.42 TIDD1356 pKa = 3.77 NMDD1359 pKa = 3.73 KK1360 pKa = 11.53 LEE1362 pKa = 4.06 VNNDD1366 pKa = 3.66 FKK1368 pKa = 11.7 DD1369 pKa = 3.19 KK1370 pKa = 10.82 DD1371 pKa = 3.9 YY1372 pKa = 11.22 PDD1374 pKa = 3.57 WLLGIMALSVGKK1386 pKa = 10.74 VYY1388 pKa = 9.25 LTKK1391 pKa = 10.33 QDD1393 pKa = 3.69 AYY1395 pKa = 9.36 TGAEE1399 pKa = 4.56 GYY1401 pKa = 9.81 IGSCATVNYY1410 pKa = 9.86 AGKK1413 pKa = 9.79 LHH1415 pKa = 7.34 IITNKK1420 pKa = 9.12 HH1421 pKa = 3.66 VVMGYY1426 pKa = 10.44 GKK1428 pKa = 9.3 EE1429 pKa = 3.92 HH1430 pKa = 7.3 PLRR1433 pKa = 11.84 TKK1435 pKa = 10.87 SSIGYY1440 pKa = 9.65 VGTHH1444 pKa = 6.1 KK1445 pKa = 10.76 NVEE1448 pKa = 4.15 ARR1450 pKa = 11.84 FMLQGKK1456 pKa = 8.81 VYY1458 pKa = 9.02 NVQMEE1463 pKa = 4.49 TLVAAGDD1470 pKa = 3.99 DD1471 pKa = 3.78 FVLYY1475 pKa = 10.82 SIDD1478 pKa = 3.53 SEE1480 pKa = 4.34 MEE1482 pKa = 3.7 RR1483 pKa = 11.84 RR1484 pKa = 11.84 LSRR1487 pKa = 11.84 ALGPIGIQEE1496 pKa = 4.1 AVNYY1500 pKa = 9.28 FPSFSEE1506 pKa = 4.06 NMMKK1510 pKa = 9.95 TGIYY1514 pKa = 10.25 LSATRR1519 pKa = 11.84 QTDD1522 pKa = 3.36 RR1523 pKa = 11.84 TIDD1526 pKa = 3.53 DD1527 pKa = 3.85 EE1528 pKa = 4.42 MNKK1531 pKa = 9.68 ISLRR1535 pKa = 11.84 NTLVQWDD1542 pKa = 4.27 SARR1545 pKa = 11.84 QIYY1548 pKa = 10.32 NILGYY1553 pKa = 6.37 TTQGMSGSPIFHH1565 pKa = 7.21 IEE1567 pKa = 3.48 QDD1569 pKa = 3.32 AKK1571 pKa = 9.51 RR1572 pKa = 11.84 TKK1574 pKa = 10.16 IYY1576 pKa = 10.84 LVGLNSAVEE1585 pKa = 4.18 SLEE1588 pKa = 3.65 RR1589 pKa = 11.84 HH1590 pKa = 4.43 QVHH1593 pKa = 5.52 MTRR1596 pKa = 11.84 IIDD1599 pKa = 3.45 AFKK1602 pKa = 10.81 IKK1604 pKa = 10.23 DD1605 pKa = 3.53 VCDD1608 pKa = 3.67 SFSKK1612 pKa = 11.01 GNARR1616 pKa = 11.84 SVNEE1620 pKa = 3.93 NCILPCLTEE1629 pKa = 3.79 QLQFDD1634 pKa = 3.95 MQQAYY1639 pKa = 10.48 SINFCGIDD1647 pKa = 3.86 VEE1649 pKa = 4.7 EE1650 pKa = 4.38 KK1651 pKa = 9.28 WGGGIWPILKK1661 pKa = 10.57 NNTLLCNKK1669 pKa = 9.64 GKK1671 pKa = 10.55 YY1672 pKa = 9.64 DD1673 pKa = 3.84 EE1674 pKa = 4.32 QKK1676 pKa = 9.91 KK1677 pKa = 9.56 AQYY1680 pKa = 9.19 FWNIYY1685 pKa = 6.54 NHH1687 pKa = 5.82 EE1688 pKa = 4.25 MMMSEE1693 pKa = 4.31 SPAKK1697 pKa = 9.47 IDD1699 pKa = 3.44 HH1700 pKa = 6.96 DD1701 pKa = 4.18 LVSEE1705 pKa = 4.15 MFEE1708 pKa = 4.31 SCAVTTDD1715 pKa = 3.05 IKK1717 pKa = 10.94 HH1718 pKa = 5.99 EE1719 pKa = 4.15 KK1720 pKa = 10.09 LLEE1723 pKa = 4.75 IDD1725 pKa = 3.37 MRR1727 pKa = 11.84 SEE1729 pKa = 4.58 GFCMMDD1735 pKa = 3.09 MLKK1738 pKa = 10.43 LINIIRR1744 pKa = 11.84 TKK1746 pKa = 10.57 DD1747 pKa = 3.24 SKK1749 pKa = 11.39 GKK1751 pKa = 9.89 SRR1753 pKa = 11.84 YY1754 pKa = 9.89 DD1755 pKa = 3.05 GFSFRR1760 pKa = 11.84 WNNLKK1765 pKa = 10.19 EE1766 pKa = 4.02 YY1767 pKa = 10.85 KK1768 pKa = 10.3 FINNSSDD1775 pKa = 2.56 TWIFNIQICKK1785 pKa = 9.78 QDD1787 pKa = 2.95 IDD1789 pKa = 3.5 NRR1791 pKa = 11.84 TIIVEE1796 pKa = 4.24 DD1797 pKa = 3.67 NFLHH1801 pKa = 6.98 SFFASIEE1808 pKa = 3.98 SDD1810 pKa = 3.37 DD1811 pKa = 5.26 DD1812 pKa = 3.57 YY1813 pKa = 10.77 TTIYY1817 pKa = 11.21 DD1818 pKa = 4.14 KK1819 pKa = 11.17 VLKK1822 pKa = 9.97 NAPIDD1827 pKa = 3.8 KK1828 pKa = 10.31 VVTGTWLGNQLMVTTYY1844 pKa = 10.99 DD1845 pKa = 3.05 KK1846 pKa = 11.63 KK1847 pKa = 11.06 EE1848 pKa = 4.11 FNLCTTGTLGTLYY1861 pKa = 9.24 MYY1863 pKa = 10.7 CGEE1866 pKa = 4.7 LDD1868 pKa = 3.47 KK1869 pKa = 10.95 KK1870 pKa = 10.56 IPSVPVTEE1878 pKa = 5.41 EE1879 pKa = 3.61 EE1880 pKa = 5.37 FEE1882 pKa = 4.25 DD1883 pKa = 5.12 LKK1885 pKa = 11.58 NNPDD1889 pKa = 3.43 KK1890 pKa = 11.07 RR1891 pKa = 11.84 QEE1893 pKa = 3.6 IYY1895 pKa = 11.22 NRR1897 pKa = 11.84 IKK1899 pKa = 10.64 NLKK1902 pKa = 8.6 EE1903 pKa = 3.9 DD1904 pKa = 3.84 KK1905 pKa = 10.69 KK1906 pKa = 11.09 QIAADD1911 pKa = 3.77 LEE1913 pKa = 4.38 KK1914 pKa = 10.53 KK1915 pKa = 9.91 RR1916 pKa = 11.84 RR1917 pKa = 11.84 QITDD1921 pKa = 2.92 EE1922 pKa = 4.07 KK1923 pKa = 10.91 QSYY1926 pKa = 10.0 KK1927 pKa = 10.64 YY1928 pKa = 10.51 LEE1930 pKa = 4.2 KK1931 pKa = 10.78 ANEE1934 pKa = 4.11 YY1935 pKa = 10.47 EE1936 pKa = 5.23 SVINDD1941 pKa = 3.23 YY1942 pKa = 10.62 KK1943 pKa = 11.19 KK1944 pKa = 10.29 KK1945 pKa = 10.62 VKK1947 pKa = 10.27 EE1948 pKa = 3.85 FEE1950 pKa = 4.22 QNNKK1954 pKa = 8.59 NVQGLLQEE1962 pKa = 5.15 LKK1964 pKa = 10.15 AQKK1967 pKa = 10.45 KK1968 pKa = 8.22 EE1969 pKa = 3.38 IEE1971 pKa = 3.9 MRR1973 pKa = 11.84 YY1974 pKa = 8.23 NHH1976 pKa = 7.07 LMQEE1980 pKa = 3.88 KK1981 pKa = 10.35 RR1982 pKa = 11.84 NNKK1985 pKa = 8.66 KK1986 pKa = 10.69 DD1987 pKa = 3.61 KK1988 pKa = 10.77 IEE1990 pKa = 3.88 EE1991 pKa = 4.04 AKK1993 pKa = 10.49 KK1994 pKa = 10.03 EE1995 pKa = 3.81 RR1996 pKa = 11.84 EE1997 pKa = 3.95 AFKK2000 pKa = 11.06 NEE2002 pKa = 3.53 EE2003 pKa = 4.0 KK2004 pKa = 11.02 GIIDD2008 pKa = 4.56 EE2009 pKa = 4.3 IEE2011 pKa = 3.96 KK2012 pKa = 10.59 KK2013 pKa = 10.49 INEE2016 pKa = 3.77 LRR2018 pKa = 11.84 GNINEE2023 pKa = 4.36 SKK2025 pKa = 10.43 EE2026 pKa = 3.99 QVKK2029 pKa = 9.85 QLIEE2033 pKa = 3.93 SRR2035 pKa = 11.84 RR2036 pKa = 11.84 LIGEE2040 pKa = 4.16 NNHH2043 pKa = 6.24 HH2044 pKa = 6.09 KK2045 pKa = 10.46 RR2046 pKa = 11.84 KK2047 pKa = 10.11 EE2048 pKa = 3.81 IFLKK2052 pKa = 11.13 NEE2054 pKa = 3.54 IEE2056 pKa = 4.02 EE2057 pKa = 4.19 LTRR2060 pKa = 11.84 EE2061 pKa = 3.95 IEE2063 pKa = 3.84 ALQIKK2068 pKa = 10.21 KK2069 pKa = 10.21 EE2070 pKa = 4.19 DD2071 pKa = 3.57 EE2072 pKa = 4.14 LKK2074 pKa = 10.96 VIKK2077 pKa = 10.35 QKK2079 pKa = 10.99 LKK2081 pKa = 10.38 EE2082 pKa = 4.14 NQIPPPEE2089 pKa = 5.08 KK2090 pKa = 9.89 PDD2092 pKa = 3.37 VQNPEE2097 pKa = 4.58 LSPEE2101 pKa = 3.96 MTQMRR2106 pKa = 11.84 KK2107 pKa = 8.92 EE2108 pKa = 4.05 VTNSRR2113 pKa = 11.84 KK2114 pKa = 9.08 MIRR2117 pKa = 11.84 DD2118 pKa = 3.05 IDD2120 pKa = 3.81 QKK2122 pKa = 10.74 IDD2124 pKa = 3.4 EE2125 pKa = 5.03 LKK2127 pKa = 10.42 TMLEE2131 pKa = 4.12 HH2132 pKa = 7.74 KK2133 pKa = 10.43 SINLNSKK2140 pKa = 8.06 VTVANVQVVTDD2151 pKa = 4.07 KK2152 pKa = 11.26 VKK2154 pKa = 10.69 QINDD2158 pKa = 3.45 FVEE2161 pKa = 4.4 QQCRR2165 pKa = 11.84 TIQTEE2170 pKa = 3.83 DD2171 pKa = 3.74 GYY2173 pKa = 11.68 NSLMEE2178 pKa = 4.13 TATRR2182 pKa = 11.84 LIDD2185 pKa = 4.45 SLEE2188 pKa = 3.8 QDD2190 pKa = 3.92 LLDD2193 pKa = 5.68 DD2194 pKa = 6.07 FEE2196 pKa = 6.26 ALQNSYY2202 pKa = 11.66 YY2203 pKa = 10.99 NLLEE2207 pKa = 4.39 KK2208 pKa = 10.99 DD2209 pKa = 3.12 PFEE2212 pKa = 3.87 EE2213 pKa = 4.12 VKK2215 pKa = 10.42 RR2216 pKa = 11.84 KK2217 pKa = 9.91 KK2218 pKa = 10.22 KK2219 pKa = 9.76 SQPRR2223 pKa = 11.84 SDD2225 pKa = 3.44 EE2226 pKa = 4.01 EE2227 pKa = 4.03 KK2228 pKa = 10.32 NLRR2231 pKa = 11.84 KK2232 pKa = 9.15 RR2233 pKa = 11.84 DD2234 pKa = 3.64 YY2235 pKa = 11.14 DD2236 pKa = 3.8 RR2237 pKa = 11.84 EE2238 pKa = 3.96 EE2239 pKa = 4.05 LQNSGEE2245 pKa = 4.09 EE2246 pKa = 4.03 RR2247 pKa = 11.84 DD2248 pKa = 3.73 QKK2250 pKa = 11.29 KK2251 pKa = 9.0 PWRR2254 pKa = 11.84 GNRR2257 pKa = 11.84 FEE2259 pKa = 4.71 VLNSLPKK2266 pKa = 9.63 DD2267 pKa = 3.92 TQPSTSKK2274 pKa = 10.92 DD2275 pKa = 3.59 SEE2277 pKa = 4.54 TKK2279 pKa = 10.05 QQDD2282 pKa = 3.02 IPIEE2286 pKa = 3.84 ISIEE2290 pKa = 4.01 EE2291 pKa = 4.31 MEE2293 pKa = 5.51 KK2294 pKa = 10.29 MMKK2297 pKa = 9.96 GQKK2300 pKa = 9.3 QEE2302 pKa = 4.19 SKK2304 pKa = 10.65 KK2305 pKa = 10.81 LRR2307 pKa = 11.84 DD2308 pKa = 3.27 RR2309 pKa = 11.84 TYY2311 pKa = 11.64 DD2312 pKa = 3.11 RR2313 pKa = 11.84 VKK2315 pKa = 10.23 TVLKK2319 pKa = 10.27 QFKK2322 pKa = 9.56 NGHH2325 pKa = 5.08 KK2326 pKa = 9.59 TFDD2329 pKa = 3.03 NWGQRR2334 pKa = 11.84 IDD2336 pKa = 3.46 GKK2338 pKa = 10.07 FYY2340 pKa = 10.54 RR2341 pKa = 11.84 IKK2343 pKa = 10.32 IEE2345 pKa = 4.53 KK2346 pKa = 9.67 KK2347 pKa = 9.86 DD2348 pKa = 3.65 LPKK2351 pKa = 10.5 LVEE2354 pKa = 3.92 IQRR2357 pKa = 11.84 EE2358 pKa = 4.04 FCEE2361 pKa = 4.3 NEE2363 pKa = 3.62 WEE2365 pKa = 4.04 ISKK2368 pKa = 11.33 AEE2370 pKa = 4.46 LDD2372 pKa = 3.56 EE2373 pKa = 4.82 AMNGPVEE2380 pKa = 4.62 KK2381 pKa = 10.68 KK2382 pKa = 9.91 FANQFLGTNRR2392 pKa = 11.84 YY2393 pKa = 8.89 IKK2395 pKa = 9.84 MRR2397 pKa = 11.84 LEE2399 pKa = 4.25 SKK2401 pKa = 9.2 TDD2403 pKa = 3.12 KK2404 pKa = 10.8 AQNGIVNMIFSTEE2417 pKa = 3.65 SKK2419 pKa = 9.98 ATVKK2423 pKa = 10.69 SQIEE2427 pKa = 3.89 QGRR2430 pKa = 11.84 TEE2432 pKa = 4.17 SEE2434 pKa = 3.61 LMRR2437 pKa = 11.84 NNNYY2441 pKa = 10.71 AIFDD2445 pKa = 4.15 KK2446 pKa = 11.0 QEE2448 pKa = 3.7 QWPGWAASLVDD2459 pKa = 3.24 NWFKK2463 pKa = 11.08 NEE2465 pKa = 4.04 KK2466 pKa = 9.86 IIPIWARR2473 pKa = 11.84 QLSVRR2478 pKa = 11.84 QISEE2482 pKa = 3.85 KK2483 pKa = 10.06 FVTVIQVSDD2492 pKa = 3.27 KK2493 pKa = 10.55 MKK2495 pKa = 10.25 PLLTNEE2501 pKa = 3.73 QDD2503 pKa = 3.94 SNSFLYY2509 pKa = 9.53 STVVPRR2515 pKa = 11.84 TRR2517 pKa = 11.84 KK2518 pKa = 9.18 VNWRR2522 pKa = 11.84 VEE2524 pKa = 4.18 LTDD2527 pKa = 3.47 VFPVKK2532 pKa = 10.73 DD2533 pKa = 3.53 KK2534 pKa = 10.87 LRR2536 pKa = 11.84 KK2537 pKa = 9.18 NRR2539 pKa = 11.84 AHH2541 pKa = 6.42 YY2542 pKa = 10.34 EE2543 pKa = 4.09 PVFPEE2548 pKa = 3.95 YY2549 pKa = 10.99 LKK2551 pKa = 8.27 TTWVDD2556 pKa = 4.35 DD2557 pKa = 3.86 MILYY2561 pKa = 10.18 HH2562 pKa = 6.03 KK2563 pKa = 9.48 TKK2565 pKa = 10.9 DD2566 pKa = 3.52 DD2567 pKa = 3.84 FKK2569 pKa = 11.46 SYY2571 pKa = 10.35 ISHH2574 pKa = 6.16 EE2575 pKa = 4.17 QQLVHH2580 pKa = 7.44 VSDD2583 pKa = 4.2 EE2584 pKa = 3.86 NFEE2587 pKa = 4.34 IIKK2590 pKa = 9.89 PIIDD2594 pKa = 3.82 EE2595 pKa = 4.19 LHH2597 pKa = 6.95 DD2598 pKa = 3.44 KK2599 pKa = 10.76 HH2600 pKa = 6.73 FKK2602 pKa = 10.49 KK2603 pKa = 10.87 FNNSLSWDD2611 pKa = 4.08 TFDD2614 pKa = 3.93 HH2615 pKa = 5.7 TTGQKK2620 pKa = 9.36 IKK2622 pKa = 10.46 QIAEE2626 pKa = 3.62 IDD2628 pKa = 3.66 VEE2630 pKa = 5.15 DD2631 pKa = 3.7 NFKK2634 pKa = 10.92 RR2635 pKa = 11.84 ITDD2638 pKa = 3.51 SKK2640 pKa = 10.87 GQIFQDD2646 pKa = 3.66 VNSLLKK2652 pKa = 9.31 YY2653 pKa = 8.55 TNHH2656 pKa = 6.42 DD2657 pKa = 3.86 HH2658 pKa = 7.75 IITEE2662 pKa = 4.79 KK2663 pKa = 9.6 IHH2665 pKa = 5.82 KK2666 pKa = 9.0 QIPEE2670 pKa = 4.18 EE2671 pKa = 3.72 IDD2673 pKa = 3.25 YY2674 pKa = 11.4 VKK2676 pKa = 10.29 IYY2678 pKa = 9.67 TLSDD2682 pKa = 2.88 ARR2684 pKa = 11.84 NNIINGFNKK2693 pKa = 9.93 IADD2696 pKa = 3.5 SGYY2699 pKa = 10.41 VHH2701 pKa = 7.02 EE2702 pKa = 5.63 GWDD2705 pKa = 3.52 KK2706 pKa = 11.67 NIGFDD2711 pKa = 4.24 LDD2713 pKa = 3.73 EE2714 pKa = 4.97 FNISIRR2720 pKa = 11.84 SYY2722 pKa = 9.71 KK2723 pKa = 7.96 TAQYY2727 pKa = 7.41 PTVGKK2732 pKa = 10.72 YY2733 pKa = 7.24 YY2734 pKa = 10.54 EE2735 pKa = 4.32 RR2736 pKa = 11.84 YY2737 pKa = 9.86 KK2738 pKa = 11.23 NLINNPDD2745 pKa = 3.53 TAIEE2749 pKa = 4.47 SIVIDD2754 pKa = 3.87 NVMEE2758 pKa = 4.58 DD2759 pKa = 3.69 LLIEE2763 pKa = 4.54 KK2764 pKa = 10.1 GFNTQQLLSNSDD2776 pKa = 3.66 YY2777 pKa = 11.37 YY2778 pKa = 10.11 ITEE2781 pKa = 3.78 KK2782 pKa = 10.88 NIYY2785 pKa = 9.66 HH2786 pKa = 6.19 LTDD2789 pKa = 4.19 SIARR2793 pKa = 11.84 YY2794 pKa = 9.32 NLSPLPRR2801 pKa = 11.84 SEE2803 pKa = 4.35 QLQNDD2808 pKa = 3.13 VDD2810 pKa = 3.9 EE2811 pKa = 5.48 LKK2813 pKa = 10.91 FFAYY2817 pKa = 10.08 CLYY2820 pKa = 11.22 NNDD2823 pKa = 3.75 RR2824 pKa = 11.84 KK2825 pKa = 10.41 IGLSGFRR2832 pKa = 11.84 PDD2834 pKa = 5.06 SFIKK2838 pKa = 10.99 VNGQSAPGYY2847 pKa = 8.64 GLNRR2851 pKa = 11.84 IFKK2854 pKa = 10.58 SNEE2857 pKa = 3.41 NLKK2860 pKa = 10.77 DD2861 pKa = 3.74 FLPAYY2866 pKa = 9.69 VDD2868 pKa = 3.92 YY2869 pKa = 11.62 YY2870 pKa = 11.25 HH2871 pKa = 7.55 DD2872 pKa = 4.32 LVLNYY2877 pKa = 10.32 GYY2879 pKa = 10.12 IPSCIYY2885 pKa = 10.61 KK2886 pKa = 10.21 SFEE2889 pKa = 3.95 KK2890 pKa = 10.91 LCVQKK2895 pKa = 10.73 KK2896 pKa = 9.27 EE2897 pKa = 4.08 KK2898 pKa = 10.06 IKK2900 pKa = 10.32 EE2901 pKa = 3.58 IRR2903 pKa = 11.84 TISGAPSWLSALGKK2917 pKa = 10.24 QYY2919 pKa = 11.43 AHH2921 pKa = 7.24 PEE2923 pKa = 3.47 EE2924 pKa = 4.79 SLYY2927 pKa = 9.82 QKK2929 pKa = 10.81 CMNEE2933 pKa = 3.98 TTLKK2937 pKa = 10.59 LGQNFYY2943 pKa = 11.48 GGGFDD2948 pKa = 4.19 YY2949 pKa = 10.58 FAEE2952 pKa = 4.21 EE2953 pKa = 4.62 LLASSNEE2960 pKa = 3.86 QILYY2964 pKa = 7.56 RR2965 pKa = 11.84 TSDD2968 pKa = 3.39 FSKK2971 pKa = 10.9 NDD2973 pKa = 3.13 IFEE2976 pKa = 4.2 HH2977 pKa = 6.65 VYY2979 pKa = 10.42 VKK2981 pKa = 10.64 EE2982 pKa = 4.46 LISKK2986 pKa = 9.73 NRR2988 pKa = 11.84 IEE2990 pKa = 4.18 VSGFTDD2996 pKa = 3.65 EE2997 pKa = 4.46 EE2998 pKa = 4.27 KK2999 pKa = 10.67 KK3000 pKa = 10.51 IYY3002 pKa = 10.06 CALHH3006 pKa = 5.91 EE3007 pKa = 4.88 NNTNFIRR3014 pKa = 11.84 STPWGEE3020 pKa = 3.99 LYY3022 pKa = 10.39 QVEE3025 pKa = 4.39 TGKK3028 pKa = 10.82 ASGSPWTCIDD3038 pKa = 3.18 NCKK3041 pKa = 10.15 KK3042 pKa = 10.07 HH3043 pKa = 7.03 DD3044 pKa = 4.74 GILYY3048 pKa = 8.41 VQAAKK3053 pKa = 9.59 MICKK3057 pKa = 10.19 EE3058 pKa = 3.75 KK3059 pKa = 10.73 DD3060 pKa = 2.98 IEE3062 pKa = 4.31 FKK3064 pKa = 10.62 PDD3066 pKa = 3.4 NFKK3069 pKa = 11.09 LLYY3072 pKa = 10.6 NIICQGDD3079 pKa = 3.95 EE3080 pKa = 3.84 IDD3082 pKa = 5.25 FKK3084 pKa = 11.33 CFSDD3088 pKa = 4.39 DD3089 pKa = 4.59 AIHH3092 pKa = 6.5 KK3093 pKa = 7.46 VKK3095 pKa = 10.19 PVYY3098 pKa = 10.32 VKK3100 pKa = 10.27 YY3101 pKa = 10.35 FINGEE3106 pKa = 3.77 QDD3108 pKa = 2.88 IEE3110 pKa = 4.39 TKK3112 pKa = 10.14 INKK3115 pKa = 9.08 NMGVLIKK3122 pKa = 10.17 PNKK3125 pKa = 8.37 ISEE3128 pKa = 4.25 WTDD3131 pKa = 2.91 KK3132 pKa = 11.15 LDD3134 pKa = 3.5 YY3135 pKa = 11.17 DD3136 pKa = 4.03 FEE3138 pKa = 5.11 KK3139 pKa = 11.05 KK3140 pKa = 10.28 EE3141 pKa = 4.34 GICWLGNHH3149 pKa = 5.11 FTKK3152 pKa = 11.08 YY3153 pKa = 8.64 EE3154 pKa = 4.24 GKK3156 pKa = 8.74 WYY3158 pKa = 9.16 PIRR3161 pKa = 11.84 SEE3163 pKa = 4.11 EE3164 pKa = 4.06 KK3165 pKa = 10.07 VLFGLLSINPEE3176 pKa = 3.87 SRR3178 pKa = 11.84 VGKK3181 pKa = 9.89 DD3182 pKa = 3.03 VNLNPDD3188 pKa = 3.09 TTLAYY3193 pKa = 10.0 SYY3195 pKa = 11.01 RR3196 pKa = 11.84 LAKK3199 pKa = 10.41 LFSFMLFVVPNEE3211 pKa = 4.09 RR3212 pKa = 11.84 VFKK3215 pKa = 10.56 LLEE3218 pKa = 4.3 TIRR3221 pKa = 11.84 SEE3223 pKa = 4.04 VVQRR3227 pKa = 11.84 IKK3229 pKa = 11.26 AKK3231 pKa = 10.84 VPDD3234 pKa = 4.43 TKK3236 pKa = 11.22 LSMYY3240 pKa = 10.95 DD3241 pKa = 3.39 LLEE3244 pKa = 4.31 KK3245 pKa = 10.49 VDD3247 pKa = 3.97 EE3248 pKa = 4.75 KK3249 pKa = 11.15 FVSLWIAAEE3258 pKa = 4.1 KK3259 pKa = 11.08 VKK3261 pKa = 10.52 MEE3263 pKa = 5.49 DD3264 pKa = 3.64 KK3265 pKa = 11.29 NCDD3268 pKa = 2.91 IWSHH3272 pKa = 5.34 TDD3274 pKa = 3.52 FKK3276 pKa = 11.7 NFIGKK3281 pKa = 9.76 EE3282 pKa = 3.47 ILLYY3286 pKa = 10.69 TNTVKK3291 pKa = 10.87 LNALIYY3297 pKa = 8.96 EE3298 pKa = 4.52 GQRR3301 pKa = 11.84 TGFDD3305 pKa = 3.57 FSHH3308 pKa = 6.83 GSNVIIDD3315 pKa = 4.25 LLKK3318 pKa = 10.55 EE3319 pKa = 4.17 SNVEE3323 pKa = 3.54 IKK3325 pKa = 10.67 EE3326 pKa = 4.08 NLEE3329 pKa = 4.09 STILAANHH3337 pKa = 5.94 VDD3339 pKa = 3.85 DD3340 pKa = 4.77 IFMRR3344 pKa = 11.84 KK3345 pKa = 8.27 QMPFAHH3351 pKa = 6.17 VNNKK3355 pKa = 9.74 NIPKK3359 pKa = 9.88 ILSGLMNTVYY3369 pKa = 10.91 NSTCDD3374 pKa = 3.7 PEE3376 pKa = 4.54 VLNMRR3381 pKa = 11.84 INNFLSIRR3389 pKa = 11.84 LHH3391 pKa = 6.59 IDD3393 pKa = 2.99 YY3394 pKa = 10.53 DD3395 pKa = 3.66 IEE3397 pKa = 4.31 QINQLGYY3404 pKa = 8.43 KK3405 pKa = 9.08 TVVLTQKK3412 pKa = 10.99 NIINYY3417 pKa = 9.22 KK3418 pKa = 10.43 KK3419 pKa = 10.05 IGTDD3423 pKa = 3.08 VRR3425 pKa = 11.84 LLIALPRR3432 pKa = 11.84 LNEE3435 pKa = 3.57 IQNIYY3440 pKa = 10.69 SIAQLFNGRR3449 pKa = 11.84 SYY3451 pKa = 10.99 IYY3453 pKa = 10.14 IEE3455 pKa = 3.85 TDD3457 pKa = 2.89 KK3458 pKa = 11.02 VHH3460 pKa = 6.93 KK3461 pKa = 10.6 VDD3463 pKa = 5.61 LSYY3466 pKa = 11.54 LFDD3469 pKa = 3.99 NFEE3472 pKa = 4.0 QKK3474 pKa = 10.45 NRR3476 pKa = 11.84 WLIHH3480 pKa = 5.9 EE3481 pKa = 4.4 GTYY3484 pKa = 10.3 LYY3486 pKa = 10.65 EE3487 pKa = 4.62 FKK3489 pKa = 10.68 RR3490 pKa = 11.84 RR3491 pKa = 11.84 NNTIEE3496 pKa = 3.69 RR3497 pKa = 11.84 TLNYY3501 pKa = 9.3 SACSSYY3507 pKa = 10.37 STDD3510 pKa = 3.23 EE3511 pKa = 4.08 FNKK3514 pKa = 10.47 EE3515 pKa = 3.36 NLQFFEE3521 pKa = 4.96 KK3522 pKa = 10.54 DD3523 pKa = 3.14 KK3524 pKa = 11.15 KK3525 pKa = 11.03 DD3526 pKa = 3.47 PNAFKK3531 pKa = 10.56 DD3532 pKa = 3.54 YY3533 pKa = 10.71 QYY3535 pKa = 10.82 RR3536 pKa = 11.84 KK3537 pKa = 10.51 NMLEE3541 pKa = 4.08 HH3542 pKa = 7.13 YY3543 pKa = 8.64 EE3544 pKa = 4.34 VNCDD3548 pKa = 3.42 PRR3550 pKa = 11.84 FVEE3553 pKa = 4.39 YY3554 pKa = 9.85 MLHH3557 pKa = 6.69 HH3558 pKa = 6.48 EE3559 pKa = 4.13 QTICEE3564 pKa = 4.28 NFNVDD3569 pKa = 3.34 QRR3571 pKa = 11.84 EE3572 pKa = 4.36 SNHH3575 pKa = 6.66 FIQSINGGPGTGKK3588 pKa = 5.76 TTKK3591 pKa = 10.34 LIEE3594 pKa = 4.46 VIEE3597 pKa = 4.0 NLKK3600 pKa = 10.67 DD3601 pKa = 3.42 KK3602 pKa = 10.75 NYY3604 pKa = 10.47 KK3605 pKa = 10.17 LLVVASANEE3614 pKa = 3.92 QISNIGNRR3622 pKa = 11.84 MMKK3625 pKa = 10.53 KK3626 pKa = 9.73 NMKK3629 pKa = 9.99 FGMSVSQKK3637 pKa = 10.3 ARR3639 pKa = 11.84 EE3640 pKa = 4.15 LNLVPQDD3647 pKa = 3.72 LLNYY3651 pKa = 6.77 TNKK3654 pKa = 10.16 YY3655 pKa = 8.42 KK3656 pKa = 10.76 RR3657 pKa = 11.84 SDD3659 pKa = 3.04 IMLCTMHH3666 pKa = 6.84 TAYY3669 pKa = 9.4 TKK3671 pKa = 10.37 KK3672 pKa = 10.21 YY3673 pKa = 8.55 RR3674 pKa = 11.84 SDD3676 pKa = 3.64 IILVDD3681 pKa = 3.18 EE3682 pKa = 4.78 CSRR3685 pKa = 11.84 VTIAEE3690 pKa = 4.13 MVLFLCRR3697 pKa = 11.84 VSPFYY3702 pKa = 10.87 SKK3704 pKa = 10.63 HH3705 pKa = 4.52 VNYY3708 pKa = 10.01 DD3709 pKa = 3.26 VKK3711 pKa = 10.86 KK3712 pKa = 10.68 LYY3714 pKa = 11.05 LFGDD3718 pKa = 4.23 KK3719 pKa = 10.66 YY3720 pKa = 9.71 QANVFNPDD3728 pKa = 3.16 NLQTNFMQPLSSLIPTEE3745 pKa = 3.79 DD3746 pKa = 3.53 TIFLNRR3752 pKa = 11.84 TYY3754 pKa = 10.97 RR3755 pKa = 11.84 FGPNIVEE3762 pKa = 4.12 ILNSIAYY3769 pKa = 10.05 AEE3771 pKa = 4.47 LPLVAEE3777 pKa = 4.7 KK3778 pKa = 11.04 KK3779 pKa = 10.23 EE3780 pKa = 3.87 DD3781 pKa = 3.52 TKK3783 pKa = 10.95 IDD3785 pKa = 3.57 TRR3787 pKa = 11.84 FTCYY3791 pKa = 9.46 IGKK3794 pKa = 9.64 QIHH3797 pKa = 6.03 HH3798 pKa = 6.89 EE3799 pKa = 4.08 KK3800 pKa = 10.44 LSEE3803 pKa = 4.03 INGEE3807 pKa = 3.76 QSYY3810 pKa = 11.26 VNIEE3814 pKa = 3.69 NARR3817 pKa = 11.84 YY3818 pKa = 10.19 VMEE3821 pKa = 4.34 SLKK3824 pKa = 10.58 KK3825 pKa = 8.23 EE3826 pKa = 4.02 HH3827 pKa = 6.18 GMEE3830 pKa = 3.91 SAKK3833 pKa = 10.54 IIVPYY3838 pKa = 10.29 RR3839 pKa = 11.84 GQQRR3843 pKa = 11.84 LYY3845 pKa = 8.52 KK3846 pKa = 9.69 HH3847 pKa = 6.83 HH3848 pKa = 6.6 YY3849 pKa = 8.94 NEE3851 pKa = 4.83 DD3852 pKa = 3.14 VSTIDD3857 pKa = 3.11 ASQGRR3862 pKa = 11.84 EE3863 pKa = 3.88 YY3864 pKa = 11.5 DD3865 pKa = 3.88 HH3866 pKa = 7.36 VILDD3870 pKa = 3.94 LTRR3873 pKa = 11.84 CNANGNCGFSNEE3885 pKa = 3.92 LFRR3888 pKa = 11.84 FIVSITRR3895 pKa = 11.84 CKK3897 pKa = 10.33 QSLTIVGCNKK3907 pKa = 9.41 VVNGTHH3913 pKa = 6.65 FARR3916 pKa = 11.84 VCSRR3920 pKa = 11.84 GTDD3923 pKa = 3.21 TFTKK3927 pKa = 10.38 CSQQ3930 pKa = 2.97
Molecular weight: 458.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.302
IPC2_protein 6.415
IPC_protein 6.44
Toseland 6.62
ProMoST 6.868
Dawson 6.825
Bjellqvist 6.868
Wikipedia 6.825
Rodwell 6.825
Grimsley 6.751
Solomon 6.825
Lehninger 6.825
Nozaki 7.132
DTASelect 7.234
Thurlkill 7.263
EMBOSS 7.234
Sillero 7.234
Patrickios 4.228
IPC_peptide 6.839
IPC2_peptide 6.81
IPC2.peptide.svr19 6.831
Protein with the highest isoelectric point:
>tr|A0A1L3KJ25|A0A1L3KJ25_9VIRU Putative structural polyprotein OS=Wuhan nido-like virus 1 OX=1923743 PE=4 SV=1
MM1 pKa = 7.46 NGNANMSANNQFGNRR16 pKa = 11.84 RR17 pKa = 11.84 SRR19 pKa = 11.84 SIQRR23 pKa = 11.84 SNGNNQQFPNQGQQNNNFRR42 pKa = 11.84 QQPKK46 pKa = 9.79 QGQSKK51 pKa = 9.67 PFQNPFNGNFQGYY64 pKa = 8.38 QPQQFNGNRR73 pKa = 11.84 GFSQNRR79 pKa = 11.84 SNSQTRR85 pKa = 11.84 FNQRR89 pKa = 11.84 QVSWNQQPWNQNQQNKK105 pKa = 10.0 NKK107 pKa = 8.33 VQRR110 pKa = 11.84 KK111 pKa = 8.1 IISVPSLYY119 pKa = 7.77 TTKK122 pKa = 10.46 EE123 pKa = 3.71 GRR125 pKa = 11.84 KK126 pKa = 8.07 PTISDD131 pKa = 3.3 QPFNIVVEE139 pKa = 4.59 SNNTSRR145 pKa = 11.84 YY146 pKa = 7.37 TVSQYY151 pKa = 11.65 LFMALYY157 pKa = 9.57 FSIYY161 pKa = 10.5 GPIDD165 pKa = 3.63 GKK167 pKa = 10.8 SLSGKK172 pKa = 9.73 RR173 pKa = 11.84 IPVEE177 pKa = 3.86 TNIDD181 pKa = 3.68 HH182 pKa = 5.2 VTKK185 pKa = 10.83 DD186 pKa = 3.6 RR187 pKa = 11.84 IEE189 pKa = 3.75 NLYY192 pKa = 10.69 RR193 pKa = 11.84 GVISNLKK200 pKa = 7.82 TSKK203 pKa = 10.38 KK204 pKa = 8.32 RR205 pKa = 11.84 TEE207 pKa = 4.06 VTDD210 pKa = 5.15 DD211 pKa = 2.81 IPKK214 pKa = 10.45 RR215 pKa = 11.84 FDD217 pKa = 3.48 CLTVGNDD224 pKa = 2.99 WAKK227 pKa = 10.88 FNASDD232 pKa = 4.1 ASANGSGEE240 pKa = 3.88 QSTSKK245 pKa = 10.9 SLWDD249 pKa = 3.43
Molecular weight: 28.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.256
IPC2_protein 9.648
IPC_protein 9.94
Toseland 10.482
ProMoST 10.087
Dawson 10.613
Bjellqvist 10.248
Wikipedia 10.76
Rodwell 11.052
Grimsley 10.657
Solomon 10.657
Lehninger 10.628
Nozaki 10.452
DTASelect 10.248
Thurlkill 10.482
EMBOSS 10.877
Sillero 10.526
Patrickios 10.76
IPC_peptide 10.657
IPC2_peptide 8.887
IPC2.peptide.svr19 8.69
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
5666
249
3930
1888.7
220.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.124 ± 0.522
1.677 ± 0.435
5.542 ± 0.495
7.024 ± 0.819
5.365 ± 0.666
4.677 ± 0.339
2.153 ± 0.281
8.507 ± 0.582
9.089 ± 0.435
8.189 ± 0.759
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.665 ± 0.467
7.43 ± 1.007
2.859 ± 0.446
4.236 ± 1.137
4.059 ± 0.424
6.424 ± 0.849
5.206 ± 0.291
5.665 ± 0.408
1.306 ± 0.075
4.801 ± 0.302
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here