Gordonia phage Apricot
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 101 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345L185|A0A345L185_9CAUD Uncharacterized protein OS=Gordonia phage Apricot OX=2250319 GN=78 PE=4 SV=1
MM1 pKa = 7.6 SIADD5 pKa = 3.71 DD6 pKa = 3.73 ARR8 pKa = 11.84 AKK10 pKa = 11.0 LEE12 pKa = 4.55 GITPGSWSVDD22 pKa = 3.58 YY23 pKa = 7.56 EE24 pKa = 4.3 TSDD27 pKa = 3.44 CFPYY31 pKa = 9.49 EE32 pKa = 4.16 TYY34 pKa = 10.03 PYY36 pKa = 10.09 AISGPRR42 pKa = 11.84 NSGLDD47 pKa = 3.49 VPHH50 pKa = 7.19 VSDD53 pKa = 4.64 EE54 pKa = 3.83 YY55 pKa = 9.6 RR56 pKa = 11.84 AEE58 pKa = 4.29 YY59 pKa = 8.9 GHH61 pKa = 6.45 QMSEE65 pKa = 3.9 VCGISDD71 pKa = 3.82 QDD73 pKa = 3.84 AEE75 pKa = 4.92 FIAAAPDD82 pKa = 3.81 LVRR85 pKa = 11.84 GLLAEE90 pKa = 4.78 LDD92 pKa = 3.73 QAKK95 pKa = 10.47 GMDD98 pKa = 3.84 LSKK101 pKa = 10.52 IAAVTPCTCSAARR114 pKa = 11.84 AQRR117 pKa = 11.84 DD118 pKa = 3.28 AALATIQEE126 pKa = 4.05 VRR128 pKa = 11.84 DD129 pKa = 3.73 YY130 pKa = 10.71 CEE132 pKa = 4.83 SRR134 pKa = 11.84 ITDD137 pKa = 4.13 PGDD140 pKa = 4.15 GIDD143 pKa = 4.77 DD144 pKa = 5.27 HH145 pKa = 7.9 IDD147 pKa = 3.5 PADD150 pKa = 3.77 LLRR153 pKa = 11.84 ILGGEE158 pKa = 4.14 SS159 pKa = 2.9
Molecular weight: 17.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.972
IPC2_protein 4.075
IPC_protein 4.062
Toseland 3.859
ProMoST 4.215
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.973
Rodwell 3.897
Grimsley 3.77
Solomon 4.05
Lehninger 3.999
Nozaki 4.164
DTASelect 4.406
Thurlkill 3.897
EMBOSS 3.986
Sillero 4.19
Patrickios 2.74
IPC_peptide 4.037
IPC2_peptide 4.164
IPC2.peptide.svr19 4.086
Protein with the highest isoelectric point:
>tr|A0A345L193|A0A345L193_9CAUD Uncharacterized protein OS=Gordonia phage Apricot OX=2250319 GN=86 PE=4 SV=1
MM1 pKa = 7.69 SDD3 pKa = 2.69 ILVRR7 pKa = 11.84 RR8 pKa = 11.84 ARR10 pKa = 11.84 TRR12 pKa = 11.84 PRR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 SHH18 pKa = 6.65 KK19 pKa = 10.81 GGIHH23 pKa = 5.43 YY24 pKa = 9.87 WNVYY28 pKa = 8.49 DD29 pKa = 4.65 FRR31 pKa = 11.84 GPKK34 pKa = 9.94 VFIRR38 pKa = 11.84 QYY40 pKa = 9.25 TFSFHH45 pKa = 6.58 GAALNAAIALSKK57 pKa = 11.49 GEE59 pKa = 4.58 GINPAALFAVQRR71 pKa = 11.84 IEE73 pKa = 4.15 HH74 pKa = 6.72 GSMQDD79 pKa = 3.11 PRR81 pKa = 11.84 RR82 pKa = 11.84 DD83 pKa = 3.09 EE84 pKa = 4.47 EE85 pKa = 4.49 YY86 pKa = 10.53 CGRR89 pKa = 11.84 GVCAKK94 pKa = 10.03 MSGHH98 pKa = 7.53 PGRR101 pKa = 11.84 CGVV104 pKa = 3.49
Molecular weight: 11.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.677
IPC_protein 10.584
Toseland 10.482
ProMoST 10.277
Dawson 10.643
Bjellqvist 10.409
Wikipedia 10.891
Rodwell 10.745
Grimsley 10.716
Solomon 10.745
Lehninger 10.716
Nozaki 10.511
DTASelect 10.394
Thurlkill 10.526
EMBOSS 10.891
Sillero 10.584
Patrickios 10.467
IPC_peptide 10.745
IPC2_peptide 9.677
IPC2.peptide.svr19 8.416
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
101
0
101
16775
28
1803
166.1
18.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.772 ± 0.505
1.019 ± 0.148
6.557 ± 0.237
6.194 ± 0.299
2.343 ± 0.161
8.25 ± 0.373
2.259 ± 0.198
4.387 ± 0.16
3.356 ± 0.224
7.887 ± 0.201
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.247 ± 0.138
2.945 ± 0.187
5.621 ± 0.239
3.41 ± 0.249
7.684 ± 0.365
6.051 ± 0.228
6.671 ± 0.224
7.642 ± 0.445
2.206 ± 0.175
2.498 ± 0.124
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here