Arthrobacter phage Mendel
Average proteome isoelectric point is 6.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 28 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G3M0K4|A0A3G3M0K4_9CAUD Uncharacterized protein OS=Arthrobacter phage Mendel OX=2484218 GN=1 PE=4 SV=1
MM1 pKa = 6.74 VTTATGVFPYY11 pKa = 10.47 RR12 pKa = 11.84 LQPLNNISAFTYY24 pKa = 8.76 RR25 pKa = 11.84 TGVTYY30 pKa = 11.08 AEE32 pKa = 4.22 MLEE35 pKa = 4.03 EE36 pKa = 3.91 LRR38 pKa = 11.84 VYY40 pKa = 10.71 INDD43 pKa = 3.43 TMRR46 pKa = 11.84 PEE48 pKa = 4.2 FNSVILSVLNDD59 pKa = 3.74 FQAGIEE65 pKa = 4.1 NAEE68 pKa = 4.08 TRR70 pKa = 11.84 VVQSEE75 pKa = 4.84 TEE77 pKa = 4.23 FTALMDD83 pKa = 4.2 DD84 pKa = 3.0 ARR86 pKa = 11.84 AYY88 pKa = 11.03 VDD90 pKa = 4.69 ASVQFINNKK99 pKa = 8.31 TGQAQIQRR107 pKa = 11.84 VTLTAPYY114 pKa = 9.51 TVTIDD119 pKa = 3.7 PLWPNNHH126 pKa = 7.57 PINIVLTQDD135 pKa = 3.04 AAGGHH140 pKa = 5.81 AVTWDD145 pKa = 3.55 SEE147 pKa = 4.58 DD148 pKa = 3.38 INGSVSVSTAPNAEE162 pKa = 3.76 TDD164 pKa = 3.64 FWLVPEE170 pKa = 5.39 GDD172 pKa = 4.18 GTWTAVQYY180 pKa = 8.48 VTAVQLAGEE189 pKa = 4.33 VATINTALGTKK200 pKa = 10.05 ANSSSLATKK209 pKa = 10.12 ADD211 pKa = 3.31 KK212 pKa = 10.23 ATTITTSKK220 pKa = 9.66 PLYY223 pKa = 10.09 GIAGVIRR230 pKa = 11.84 NTGAGWDD237 pKa = 3.9 VINDD241 pKa = 3.87 AGHH244 pKa = 6.0 EE245 pKa = 4.32 PINIDD250 pKa = 3.46 NVVSDD255 pKa = 4.15 GDD257 pKa = 4.42 KK258 pKa = 9.85 ITINYY263 pKa = 6.79 GTLGASKK270 pKa = 10.47 VGTFIVTPDD279 pKa = 3.46 EE280 pKa = 4.32 KK281 pKa = 11.22 LNEE284 pKa = 3.91 QGFQVGASVGPTSTSIYY301 pKa = 9.52 LSRR304 pKa = 11.84 SIPSYY309 pKa = 10.62 SDD311 pKa = 3.2 YY312 pKa = 11.4 VAYY315 pKa = 9.69 TSGAFVSANGVFTCTWNATDD335 pKa = 5.22 GYY337 pKa = 10.64 LQVTHH342 pKa = 6.02 PTIIGSALNVSLAGRR357 pKa = 11.84 PGTALNYY364 pKa = 8.54 TPVVAGDD371 pKa = 3.86 LTTGSTFIAIEE382 pKa = 3.97 FRR384 pKa = 11.84 DD385 pKa = 3.56 AAGNQVMTPDD395 pKa = 2.86 TNMRR399 pKa = 11.84 VFVTHH404 pKa = 6.71 GGGFVPSVDD413 pKa = 3.29 PSTVTTATYY422 pKa = 10.09 PNSNLWIYY430 pKa = 11.44 AVMQDD435 pKa = 3.2
Molecular weight: 46.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.195
IPC2_protein 4.304
IPC_protein 4.279
Toseland 4.088
ProMoST 4.38
Dawson 4.253
Bjellqvist 4.431
Wikipedia 4.164
Rodwell 4.113
Grimsley 3.999
Solomon 4.253
Lehninger 4.215
Nozaki 4.368
DTASelect 4.596
Thurlkill 4.113
EMBOSS 4.177
Sillero 4.406
Patrickios 3.083
IPC_peptide 4.253
IPC2_peptide 4.38
IPC2.peptide.svr19 4.331
Protein with the highest isoelectric point:
>tr|A0A3G3M0F4|A0A3G3M0F4_9CAUD Terminase OS=Arthrobacter phage Mendel OX=2484218 GN=18 PE=4 SV=1
MM1 pKa = 7.92 GDD3 pKa = 3.42 TMHH6 pKa = 7.58 KK7 pKa = 9.8 ATATPLTQLKK17 pKa = 9.81 EE18 pKa = 3.77 RR19 pKa = 11.84 LARR22 pKa = 11.84 LGRR25 pKa = 11.84 NNLHH29 pKa = 5.97 RR30 pKa = 11.84 CYY32 pKa = 10.8 GKK34 pKa = 10.26 RR35 pKa = 11.84 CADD38 pKa = 3.68 HH39 pKa = 6.36 TNNWYY44 pKa = 10.18 AWATGTAKK52 pKa = 10.66
Molecular weight: 5.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.255
IPC2_protein 9.414
IPC_protein 9.604
Toseland 10.175
ProMoST 9.97
Dawson 10.35
Bjellqvist 10.028
Wikipedia 10.496
Rodwell 10.745
Grimsley 10.409
Solomon 10.409
Lehninger 10.379
Nozaki 10.233
DTASelect 9.999
Thurlkill 10.204
EMBOSS 10.57
Sillero 10.277
Patrickios 10.599
IPC_peptide 10.409
IPC2_peptide 9.077
IPC2.peptide.svr19 8.274
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
28
0
28
5872
40
733
209.7
22.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.746 ± 0.465
0.613 ± 0.125
6.233 ± 0.331
5.109 ± 0.5
3.883 ± 0.411
8.345 ± 0.561
1.839 ± 0.253
5.381 ± 0.311
4.155 ± 0.478
7.084 ± 0.414
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.265 ± 0.191
5.211 ± 0.471
5.126 ± 0.349
3.815 ± 0.324
4.905 ± 0.478
4.973 ± 0.256
8.328 ± 0.651
6.574 ± 0.506
1.686 ± 0.113
3.73 ± 0.361
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here