Potamochoerus porcus polyomavirus 1
Average proteome isoelectric point is 7.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S1CJI5|A0A2S1CJI5_9POLY Alto OS=Potamochoerus porcus polyomavirus 1 OX=2170410 GN=Alto PE=4 SV=1
MM1 pKa = 7.41 SLQRR5 pKa = 11.84 VWCSLRR11 pKa = 11.84 RR12 pKa = 11.84 HH13 pKa = 5.83 LKK15 pKa = 9.85 SNRR18 pKa = 11.84 LLMCKK23 pKa = 9.46 ILMEE27 pKa = 4.34 LLKK30 pKa = 10.31 MLVEE34 pKa = 4.22 LLDD37 pKa = 3.95 RR38 pKa = 11.84 GDD40 pKa = 3.85 YY41 pKa = 10.97 VDD43 pKa = 5.61 GEE45 pKa = 4.82 GQFSVDD51 pKa = 4.95 DD52 pKa = 4.06 LDD54 pKa = 5.21 ACVEE58 pKa = 4.22 YY59 pKa = 10.27 IQRR62 pKa = 11.84 LAQQAEE68 pKa = 4.27 AEE70 pKa = 4.36 EE71 pKa = 4.61 TTGEE75 pKa = 4.28 VTQEE79 pKa = 3.86 TGAGACAKK87 pKa = 9.15 QAPDD91 pKa = 3.1 EE92 pKa = 4.46 SYY94 pKa = 11.19 EE95 pKa = 4.05 FPEE98 pKa = 6.01 LIATYY103 pKa = 10.14 LASCLSLCLKK113 pKa = 10.26 AARR116 pKa = 11.84 EE117 pKa = 4.18 TTEE120 pKa = 3.71 KK121 pKa = 10.68 GRR123 pKa = 11.84 KK124 pKa = 7.86 QGARR128 pKa = 11.84 GKK130 pKa = 10.26 FKK132 pKa = 10.81 QQ133 pKa = 3.49
Molecular weight: 14.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.161
IPC2_protein 5.359
IPC_protein 5.194
Toseland 5.092
ProMoST 5.296
Dawson 5.143
Bjellqvist 5.283
Wikipedia 4.991
Rodwell 5.08
Grimsley 5.003
Solomon 5.143
Lehninger 5.105
Nozaki 5.258
DTASelect 5.359
Thurlkill 5.092
EMBOSS 5.016
Sillero 5.334
Patrickios 4.037
IPC_peptide 5.156
IPC2_peptide 5.334
IPC2.peptide.svr19 5.408
Protein with the highest isoelectric point:
>tr|A0A2S1CJI6|A0A2S1CJI6_9POLY Capsid protein VP1 OS=Potamochoerus porcus polyomavirus 1 OX=2170410 GN=VP1 PE=3 SV=1
MM1 pKa = 6.99 EE2 pKa = 4.66 QVSGTNGGRR11 pKa = 11.84 TSMSRR16 pKa = 11.84 TCIVMKK22 pKa = 10.61 SSSHH26 pKa = 5.04 QMKK29 pKa = 8.61 TAPRR33 pKa = 11.84 RR34 pKa = 11.84 VPPRR38 pKa = 11.84 VRR40 pKa = 11.84 QHH42 pKa = 4.99 RR43 pKa = 11.84 QRR45 pKa = 11.84 LRR47 pKa = 11.84 EE48 pKa = 4.25 AGPLPSSPKK57 pKa = 10.19 NLRR60 pKa = 11.84 TMM62 pKa = 4.31
Molecular weight: 7.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.217
IPC2_protein 10.438
IPC_protein 12.018
Toseland 12.179
ProMoST 12.676
Dawson 12.193
Bjellqvist 12.179
Wikipedia 12.661
Rodwell 11.857
Grimsley 12.223
Solomon 12.676
Lehninger 12.574
Nozaki 12.179
DTASelect 12.179
Thurlkill 12.179
EMBOSS 12.676
Sillero 12.179
Patrickios 11.594
IPC_peptide 12.676
IPC2_peptide 11.667
IPC2.peptide.svr19 9.282
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1824
62
616
260.6
29.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.182 ± 1.6
2.248 ± 0.646
4.167 ± 0.363
7.73 ± 0.652
3.454 ± 0.283
7.346 ± 0.647
2.248 ± 0.535
3.509 ± 0.365
5.921 ± 0.911
9.868 ± 0.45
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.686 ± 0.297
4.715 ± 0.526
6.689 ± 0.832
4.55 ± 0.331
6.469 ± 0.795
5.099 ± 0.341
5.647 ± 0.49
5.811 ± 0.485
1.371 ± 0.196
3.289 ± 0.278
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here