Hungateiclostridium clariflavum (strain DSM 19732 / NBRC 101661 / EBR45) (Clostridium clariflavum)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Acetivibrio; Acetivibrio clariflavus

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3774 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8LTS4|G8LTS4_HUNCD DNA topoisomerase OS=Hungateiclostridium clariflavum (strain DSM 19732 / NBRC 101661 / EBR45) OX=720554 GN=Clocl_4153 PE=3 SV=1
MM1 pKa = 7.58EE2 pKa = 5.93KK3 pKa = 10.47YY4 pKa = 10.01VCTVCGYY11 pKa = 10.13IYY13 pKa = 10.55DD14 pKa = 4.27PAEE17 pKa = 4.29GDD19 pKa = 3.61PDD21 pKa = 4.11GGIAPGTPFEE31 pKa = 5.98DD32 pKa = 4.8IPDD35 pKa = 3.67DD36 pKa = 3.95WVCPVCGVGKK46 pKa = 10.54DD47 pKa = 3.57MFEE50 pKa = 4.2KK51 pKa = 10.85AA52 pKa = 3.83

Molecular weight: 5.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8M2G5|G8M2G5_HUNCD 50S ribosomal protein L29 OS=Hungateiclostridium clariflavum (strain DSM 19732 / NBRC 101661 / EBR45) OX=720554 GN=rpmC PE=3 SV=1
MM1 pKa = 7.63AKK3 pKa = 10.04KK4 pKa = 10.5SLIVKK9 pKa = 7.16QQRR12 pKa = 11.84PPKK15 pKa = 9.55YY16 pKa = 8.85KK17 pKa = 8.14TRR19 pKa = 11.84AYY21 pKa = 10.26NRR23 pKa = 11.84CKK25 pKa = 10.67LCGRR29 pKa = 11.84PHH31 pKa = 7.73AYY33 pKa = 8.4MRR35 pKa = 11.84KK36 pKa = 9.41FGICRR41 pKa = 11.84LCFRR45 pKa = 11.84EE46 pKa = 4.1LAYY49 pKa = 9.85KK50 pKa = 10.19GQIPGVKK57 pKa = 9.25KK58 pKa = 10.9ASWW61 pKa = 3.03

Molecular weight: 7.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3774

0

3774

1259243

29

4062

333.7

37.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.109 ± 0.036

1.203 ± 0.016

5.76 ± 0.03

7.47 ± 0.041

4.396 ± 0.03

6.41 ± 0.035

1.366 ± 0.016

9.044 ± 0.042

8.321 ± 0.037

8.767 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.465 ± 0.019

5.665 ± 0.035

3.262 ± 0.029

2.578 ± 0.019

3.962 ± 0.027

6.27 ± 0.034

4.997 ± 0.046

6.725 ± 0.033

0.881 ± 0.014

4.351 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski