Cynomolgus macaque cytomegalovirus strain Mauritius
Average proteome isoelectric point is 7.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 290 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K1H0C2|A0A0K1H0C2_9BETA Uncharacterized protein OS=Cynomolgus macaque cytomegalovirus strain Mauritius OX=1690255 GN=Cy20 PE=4 SV=1
MM1 pKa = 7.5 RR2 pKa = 11.84 WVAMRR7 pKa = 11.84 WLLLIVATSAKK18 pKa = 10.29 ASSTTAGNTSTPTASTITSTVTTASTTNMSTPSPASSPATTNTTTSSPSTKK69 pKa = 10.33 SSTSSTNTTITTTGTASATTPKK91 pKa = 10.4 SSTSVTSSPANSTSTSTKK109 pKa = 9.78 PSTTNTSSPITTSSPASNTSTTTSSTSTVNGTNSTTEE146 pKa = 4.13 TNTTSLPDD154 pKa = 3.27 TTADD158 pKa = 3.45 MNVTTTEE165 pKa = 4.5 PYY167 pKa = 10.96 NSTGYY172 pKa = 11.03 EE173 pKa = 3.7 NVTINITTPSPYY185 pKa = 10.0 EE186 pKa = 3.61 ALQIVDD192 pKa = 4.79 LCNEE196 pKa = 4.43 TISIVFKK203 pKa = 11.14 DD204 pKa = 3.61 PGEE207 pKa = 4.06 EE208 pKa = 4.2 DD209 pKa = 3.46 EE210 pKa = 5.09 SSEE213 pKa = 3.95 TTEE216 pKa = 4.2 YY217 pKa = 11.17 SDD219 pKa = 4.21 EE220 pKa = 4.3 EE221 pKa = 4.3 PVASSNEE228 pKa = 3.83 DD229 pKa = 3.2 DD230 pKa = 3.48 STYY233 pKa = 10.3 FPQSPGYY240 pKa = 7.88 TLTYY244 pKa = 9.43 DD245 pKa = 3.78 TEE247 pKa = 4.17 DD248 pKa = 3.27 TIYY251 pKa = 10.65 FQATCDD257 pKa = 4.08 RR258 pKa = 11.84 NDD260 pKa = 3.33 TYY262 pKa = 11.56 NITSCDD268 pKa = 3.68 YY269 pKa = 10.21 TNKK272 pKa = 10.2 SVNSWSTVTSVSFFPPTLTPCHH294 pKa = 6.56 KK295 pKa = 9.56 PVAIIKK301 pKa = 9.88 IGNDD305 pKa = 3.25 SLVVSASATSNLVDD319 pKa = 5.76 AIYY322 pKa = 10.99 KK323 pKa = 10.49 LLGLPDD329 pKa = 3.78 VNSDD333 pKa = 4.72 FINQLGRR340 pKa = 11.84 YY341 pKa = 8.7 HH342 pKa = 7.95 PITLQGQIEE351 pKa = 4.19 YY352 pKa = 9.97 RR353 pKa = 11.84 DD354 pKa = 3.61 WYY356 pKa = 7.62 TTEE359 pKa = 3.67
Molecular weight: 38.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.891
IPC2_protein 4.101
IPC_protein 4.075
Toseland 3.872
ProMoST 4.177
Dawson 4.037
Bjellqvist 4.228
Wikipedia 3.948
Rodwell 3.897
Grimsley 3.783
Solomon 4.037
Lehninger 3.986
Nozaki 4.151
DTASelect 4.355
Thurlkill 3.91
EMBOSS 3.961
Sillero 4.19
Patrickios 0.757
IPC_peptide 4.037
IPC2_peptide 4.164
IPC2.peptide.svr19 4.082
Protein with the highest isoelectric point:
>tr|A0A0K1H019|A0A0K1H019_9BETA Uncharacterized protein OS=Cynomolgus macaque cytomegalovirus strain Mauritius OX=1690255 GN=Cy83 PE=4 SV=1
MM1 pKa = 6.66 VQIHH5 pKa = 6.22 EE6 pKa = 4.41 SCGTVRR12 pKa = 11.84 GPGRR16 pKa = 11.84 SRR18 pKa = 11.84 ALHH21 pKa = 6.12 RR22 pKa = 11.84 SVALLPLSDD31 pKa = 4.37 ASGDD35 pKa = 3.15 RR36 pKa = 11.84 HH37 pKa = 5.49 GRR39 pKa = 11.84 CRR41 pKa = 11.84 QDD43 pKa = 2.96 VQYY46 pKa = 11.06 SGAGCEE52 pKa = 4.09 PKK54 pKa = 10.18 LRR56 pKa = 11.84 HH57 pKa = 5.84 YY58 pKa = 10.43 RR59 pKa = 11.84 DD60 pKa = 3.09 HH61 pKa = 7.32 RR62 pKa = 11.84 NRR64 pKa = 11.84 RR65 pKa = 11.84 PEE67 pKa = 3.99 PQRR70 pKa = 11.84 DD71 pKa = 4.05 SQPHH75 pKa = 5.74 ALGAGEE81 pKa = 5.38 DD82 pKa = 3.84 DD83 pKa = 5.75 LSRR86 pKa = 11.84 LWFQQQTCASGRR98 pKa = 11.84 GRR100 pKa = 11.84 QRR102 pKa = 11.84 YY103 pKa = 9.11 SEE105 pKa = 4.53 AVPDD109 pKa = 3.59 LRR111 pKa = 11.84 VARR114 pKa = 11.84 RR115 pKa = 11.84 KK116 pKa = 9.4 HH117 pKa = 5.72 HH118 pKa = 5.79 STSASEE124 pKa = 4.1 RR125 pKa = 11.84 SLDD128 pKa = 3.5 LLAGHH133 pKa = 6.74 RR134 pKa = 11.84 RR135 pKa = 11.84 HH136 pKa = 6.0 YY137 pKa = 11.1 GKK139 pKa = 9.87 ILKK142 pKa = 10.05 YY143 pKa = 10.43 VGTKK147 pKa = 10.17 GVSS150 pKa = 2.92
Molecular weight: 16.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.347
IPC2_protein 9.619
IPC_protein 10.613
Toseland 10.54
ProMoST 10.379
Dawson 10.687
Bjellqvist 10.467
Wikipedia 10.95
Rodwell 10.716
Grimsley 10.745
Solomon 10.818
Lehninger 10.774
Nozaki 10.57
DTASelect 10.452
Thurlkill 10.57
EMBOSS 10.965
Sillero 10.628
Patrickios 10.409
IPC_peptide 10.818
IPC2_peptide 9.765
IPC2.peptide.svr19 8.492
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
290
0
290
72865
29
2179
251.3
28.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.763 ± 0.146
2.743 ± 0.104
4.451 ± 0.123
4.857 ± 0.135
4.116 ± 0.101
4.916 ± 0.158
3.318 ± 0.114
5.096 ± 0.139
3.663 ± 0.131
9.901 ± 0.171
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.665 ± 0.078
3.996 ± 0.113
5.754 ± 0.169
3.94 ± 0.134
6.903 ± 0.198
7.954 ± 0.154
6.986 ± 0.236
7.08 ± 0.148
1.433 ± 0.082
3.464 ± 0.098
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here