Carnobacterium iners
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2071 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1X7NM43|A0A1X7NM43_9LACT 4-hydroxy-tetrahydrodipicolinate synthase OS=Carnobacterium iners OX=1073423 GN=dapA PE=3 SV=1
MM1 pKa = 7.21 SHH3 pKa = 7.04 DD4 pKa = 3.98 HH5 pKa = 6.6 DD6 pKa = 4.73 HH7 pKa = 7.02 EE8 pKa = 4.28 EE9 pKa = 4.64 HH10 pKa = 5.57 EE11 pKa = 5.36 HH12 pKa = 5.2 ITIIDD17 pKa = 3.63 EE18 pKa = 4.13 QGNEE22 pKa = 3.91 EE23 pKa = 4.41 LYY25 pKa = 10.85 EE26 pKa = 3.9 ILFTFDD32 pKa = 3.6 SDD34 pKa = 4.57 DD35 pKa = 3.75 FEE37 pKa = 5.52 KK38 pKa = 10.82 SYY40 pKa = 11.56 VLVYY44 pKa = 9.75 PAGSEE49 pKa = 3.79 EE50 pKa = 4.38 GEE52 pKa = 4.23 EE53 pKa = 4.4 VEE55 pKa = 4.2 LQAYY59 pKa = 10.03 SYY61 pKa = 11.12 VEE63 pKa = 4.21 KK64 pKa = 10.75 EE65 pKa = 4.21 DD66 pKa = 4.05 GKK68 pKa = 10.98 QGDD71 pKa = 4.18 LQPIEE76 pKa = 4.5 TDD78 pKa = 3.79 EE79 pKa = 4.13 EE80 pKa = 4.17 WDD82 pKa = 3.67 MIEE85 pKa = 4.26 EE86 pKa = 4.12 VLNTFMADD94 pKa = 3.02 EE95 pKa = 4.6 DD96 pKa = 3.98
Molecular weight: 11.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.741
IPC2_protein 3.732
IPC_protein 3.694
Toseland 3.503
ProMoST 3.783
Dawson 3.656
Bjellqvist 3.846
Wikipedia 3.554
Rodwell 3.528
Grimsley 3.414
Solomon 3.643
Lehninger 3.592
Nozaki 3.77
DTASelect 3.91
Thurlkill 3.541
EMBOSS 3.567
Sillero 3.808
Patrickios 0.947
IPC_peptide 3.643
IPC2_peptide 3.783
IPC2.peptide.svr19 3.742
Protein with the highest isoelectric point:
>tr|A0A1X7NPV1|A0A1X7NPV1_9LACT DNA polymerase I OS=Carnobacterium iners OX=1073423 GN=polA PE=3 SV=1
MM1 pKa = 7.92 PKK3 pKa = 10.12 QKK5 pKa = 7.54 THH7 pKa = 6.73 RR8 pKa = 11.84 GSAKK12 pKa = 9.69 RR13 pKa = 11.84 FKK15 pKa = 10.09 RR16 pKa = 11.84 TGNGGLKK23 pKa = 9.46 RR24 pKa = 11.84 HH25 pKa = 5.83 HH26 pKa = 7.17 AKK28 pKa = 9.39 TSHH31 pKa = 5.29 MFANKK36 pKa = 8.87 SQKK39 pKa = 8.6 QKK41 pKa = 10.83 RR42 pKa = 11.84 KK43 pKa = 8.61 LRR45 pKa = 11.84 KK46 pKa = 8.72 SAMVSSGDD54 pKa = 3.46 MKK56 pKa = 10.9 RR57 pKa = 11.84 IRR59 pKa = 11.84 QQLANWW65 pKa = 3.68
Molecular weight: 7.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.359
IPC2_protein 10.804
IPC_protein 12.325
Toseland 12.515
ProMoST 12.983
Dawson 12.515
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.457
Grimsley 12.544
Solomon 12.983
Lehninger 12.896
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.179
IPC_peptide 12.998
IPC2_peptide 11.974
IPC2.peptide.svr19 8.983
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2071
0
2071
641523
40
1447
309.8
34.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.717 ± 0.055
0.531 ± 0.014
5.22 ± 0.038
7.515 ± 0.059
4.475 ± 0.038
6.461 ± 0.049
1.716 ± 0.025
8.453 ± 0.049
7.57 ± 0.049
9.921 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.699 ± 0.024
4.874 ± 0.041
3.194 ± 0.031
3.714 ± 0.037
3.665 ± 0.036
6.327 ± 0.039
5.873 ± 0.033
6.685 ± 0.036
0.819 ± 0.017
3.571 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here