Streptococcus satellite phage Javan418

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZND1|A0A4D5ZND1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan418 OX=2558693 GN=JavanS418_0008 PE=4 SV=1
MM1 pKa = 7.74IDD3 pKa = 5.03DD4 pKa = 6.14IIPEE8 pKa = 4.39LNDD11 pKa = 3.55LQTLTTVALIDD22 pKa = 4.71DD23 pKa = 4.6NLNTSQAVLRR33 pKa = 11.84VIEE36 pKa = 4.06QKK38 pKa = 10.11IPRR41 pKa = 11.84LIEE44 pKa = 3.76ILEE47 pKa = 4.17EE48 pKa = 4.27LDD50 pKa = 3.45NGG52 pKa = 4.14

Molecular weight: 5.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZSH6|A0A4D5ZSH6_9VIRU DNA primase OS=Streptococcus satellite phage Javan418 OX=2558693 GN=JavanS418_0011 PE=4 SV=1
MM1 pKa = 7.51NIKK4 pKa = 10.04EE5 pKa = 4.29VIKK8 pKa = 10.83KK9 pKa = 10.43DD10 pKa = 3.21GTKK13 pKa = 9.85VYY15 pKa = 10.42RR16 pKa = 11.84SNVYY20 pKa = 10.53LGVDD24 pKa = 3.99SITGKK29 pKa = 10.34KK30 pKa = 10.21ARR32 pKa = 11.84TSVTARR38 pKa = 11.84TKK40 pKa = 10.93KK41 pKa = 9.82EE42 pKa = 3.91LKK44 pKa = 10.19QKK46 pKa = 10.6AKK48 pKa = 10.17QAITAFINNGYY59 pKa = 5.21TTKK62 pKa = 10.34TKK64 pKa = 10.73TNIRR68 pKa = 11.84TYY70 pKa = 11.15KK71 pKa = 9.7EE72 pKa = 3.7LAYY75 pKa = 10.49LWWDD79 pKa = 3.34SCKK82 pKa = 9.88NTVKK86 pKa = 10.52INTRR90 pKa = 11.84KK91 pKa = 9.89AVEE94 pKa = 3.95GQIRR98 pKa = 11.84VHH100 pKa = 6.67LLPAFGDD107 pKa = 3.81YY108 pKa = 10.62KK109 pKa = 10.88LEE111 pKa = 4.17KK112 pKa = 9.34LTTPIIQQQVNKK124 pKa = 8.81WANDD128 pKa = 3.35YY129 pKa = 11.53NKK131 pKa = 10.46GKK133 pKa = 10.19KK134 pKa = 9.96GSYY137 pKa = 8.57KK138 pKa = 10.37HH139 pKa = 6.14YY140 pKa = 10.4NQLHH144 pKa = 6.17ALNKK148 pKa = 10.23RR149 pKa = 11.84ILQYY153 pKa = 11.28AVIMQLIPFNPARR166 pKa = 11.84EE167 pKa = 4.22VIVPRR172 pKa = 11.84KK173 pKa = 9.67KK174 pKa = 8.87QTEE177 pKa = 4.14EE178 pKa = 3.63IKK180 pKa = 10.67IKK182 pKa = 10.62FLDD185 pKa = 3.76KK186 pKa = 11.2QEE188 pKa = 4.57LKK190 pKa = 10.68QFYY193 pKa = 10.2DD194 pKa = 3.74YY195 pKa = 11.63LDD197 pKa = 3.84TLDD200 pKa = 3.24KK201 pKa = 10.91SKK203 pKa = 11.21YY204 pKa = 8.38YY205 pKa = 11.15NLFDD209 pKa = 3.55VVLYY213 pKa = 8.25KK214 pKa = 10.5TLLATGCRR222 pKa = 11.84IGEE225 pKa = 4.15VLALEE230 pKa = 4.42WSDD233 pKa = 3.44IDD235 pKa = 5.15LDD237 pKa = 3.77NGYY240 pKa = 10.33ISINKK245 pKa = 7.82TLNRR249 pKa = 11.84DD250 pKa = 3.77DD251 pKa = 4.94EE252 pKa = 5.09VNCPKK257 pKa = 10.71SKK259 pKa = 10.67AGYY262 pKa = 9.69RR263 pKa = 11.84DD264 pKa = 3.13ISIDD268 pKa = 3.12KK269 pKa = 9.51ATILMLKK276 pKa = 9.61HH277 pKa = 5.3YY278 pKa = 10.2KK279 pKa = 9.78NRR281 pKa = 11.84QTIQAWQHH289 pKa = 5.19KK290 pKa = 8.15RR291 pKa = 11.84TEE293 pKa = 4.35TVVFSTFVNKK303 pKa = 10.04YY304 pKa = 9.36ASQPALRR311 pKa = 11.84RR312 pKa = 11.84RR313 pKa = 11.84LEE315 pKa = 3.89RR316 pKa = 11.84HH317 pKa = 5.84LKK319 pKa = 8.68NAGVSYY325 pKa = 10.82VAFHH329 pKa = 6.71GLRR332 pKa = 11.84HH333 pKa = 4.61THH335 pKa = 5.07ATIMLNAGIQPKK347 pKa = 10.11DD348 pKa = 3.26LQYY351 pKa = 11.63RR352 pKa = 11.84LGHH355 pKa = 6.02SNISMTLNTYY365 pKa = 8.1VHH367 pKa = 5.2VTKK370 pKa = 10.79EE371 pKa = 3.89GAKK374 pKa = 9.74NSASFFEE381 pKa = 4.46SAINSLGG388 pKa = 3.34

Molecular weight: 44.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14

0

14

2402

52

545

171.6

19.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.495 ± 0.424

0.5 ± 0.163

6.203 ± 0.503

7.286 ± 0.986

4.163 ± 0.416

4.288 ± 0.431

1.582 ± 0.311

7.702 ± 0.501

9.992 ± 0.555

9.908 ± 0.447

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.248 ± 0.344

5.662 ± 0.413

3.122 ± 0.495

5.329 ± 0.488

4.038 ± 0.395

4.996 ± 0.352

6.495 ± 0.57

4.746 ± 0.268

1.041 ± 0.106

5.204 ± 0.362
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski