Enterococcus phage MSF2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Efquatrovirus; unclassified Efquatrovirus

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M2YBK4|A0A6M2YBK4_9CAUD XRE family transcriptional regulator OS=Enterococcus phage MSF2 OX=2577028 GN=msf2phage_53 PE=4 SV=1
MM1 pKa = 7.38YY2 pKa = 9.69IVNIIDD8 pKa = 3.68FEE10 pKa = 4.41ARR12 pKa = 11.84TLAYY16 pKa = 9.8QSEE19 pKa = 4.76VFTTKK24 pKa = 10.32EE25 pKa = 3.43LAEE28 pKa = 4.23EE29 pKa = 4.33YY30 pKa = 9.18MVEE33 pKa = 4.45VMEE36 pKa = 4.81DD37 pKa = 3.22THH39 pKa = 8.21GLDD42 pKa = 3.35VLIIEE47 pKa = 4.46VNSYY51 pKa = 10.57NLEE54 pKa = 3.87TAKK57 pKa = 10.33IVACSS62 pKa = 3.15

Molecular weight: 7.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M2YBM1|A0A6M2YBM1_9CAUD Uncharacterized protein OS=Enterococcus phage MSF2 OX=2577028 GN=msf2phage_58 PE=4 SV=1
MM1 pKa = 7.42SEE3 pKa = 3.56QWSKK7 pKa = 10.98INGYY11 pKa = 10.07DD12 pKa = 3.25GYY14 pKa = 11.1LISSYY19 pKa = 11.68GNVAYY24 pKa = 10.8NDD26 pKa = 3.93GTTKK30 pKa = 10.67KK31 pKa = 9.92PLKK34 pKa = 10.26PYY36 pKa = 10.85AKK38 pKa = 10.59DD39 pKa = 3.32NGYY42 pKa = 11.01LNADD46 pKa = 4.3LYY48 pKa = 11.71KK49 pKa = 10.57NGQRR53 pKa = 11.84TGKK56 pKa = 9.76RR57 pKa = 11.84VHH59 pKa = 6.71ILVAEE64 pKa = 4.25AFVKK68 pKa = 10.45GKK70 pKa = 10.14QEE72 pKa = 4.5GYY74 pKa = 8.61TVDD77 pKa = 3.18HH78 pKa = 7.49KK79 pKa = 11.43DD80 pKa = 3.9RR81 pKa = 11.84NRR83 pKa = 11.84HH84 pKa = 4.74NNKK87 pKa = 9.85ASNLEE92 pKa = 4.1WKK94 pKa = 10.29SVSDD98 pKa = 3.39QNRR101 pKa = 11.84NRR103 pKa = 11.84KK104 pKa = 8.37SWAKK108 pKa = 10.48GGEE111 pKa = 3.97

Molecular weight: 12.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

12364

53

1295

187.3

21.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.694 ± 0.457

0.631 ± 0.108

6.357 ± 0.269

8.007 ± 0.558

4.117 ± 0.24

6.43 ± 0.567

1.601 ± 0.193

6.891 ± 0.223

8.848 ± 0.347

8.234 ± 0.307

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.904 ± 0.217

6.406 ± 0.285

2.968 ± 0.231

3.801 ± 0.266

3.308 ± 0.137

5.071 ± 0.205

6.592 ± 0.411

6.511 ± 0.246

1.294 ± 0.157

4.335 ± 0.346
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski