Tannerella forsythia (strain ATCC 43037 / JCM 10827 / CCUG 33226 / KCTC 5666 / FDC 338) (Bacteroides forsythus)
Average proteome isoelectric point is 6.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2978 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G8UK42|G8UK42_TANFA Uncharacterized protein OS=Tannerella forsythia (strain ATCC 43037 / JCM 10827 / CCUG 33226 / KCTC 5666 / FDC 338) OX=203275 GN=BFO_0346 PE=4 SV=1
MM1 pKa = 7.48 KK2 pKa = 10.09 KK3 pKa = 10.1 YY4 pKa = 10.52 RR5 pKa = 11.84 CIVCDD10 pKa = 3.71 YY11 pKa = 10.44 IYY13 pKa = 10.89 DD14 pKa = 4.07 PVEE17 pKa = 4.26 GDD19 pKa = 3.5 PDD21 pKa = 3.9 GGIDD25 pKa = 3.74 PGTAFEE31 pKa = 6.02 DD32 pKa = 4.23 IPDD35 pKa = 3.74 DD36 pKa = 4.06 WVCPLCGVGKK46 pKa = 10.3 DD47 pKa = 3.4 DD48 pKa = 4.99 FEE50 pKa = 4.51 PVEE53 pKa = 4.05
Molecular weight: 5.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.788
IPC2_protein 3.668
IPC_protein 3.617
Toseland 3.414
ProMoST 3.783
Dawson 3.643
Bjellqvist 3.884
Wikipedia 3.617
Rodwell 3.452
Grimsley 3.325
Solomon 3.605
Lehninger 3.567
Nozaki 3.783
DTASelect 4.024
Thurlkill 3.49
EMBOSS 3.63
Sillero 3.757
Patrickios 0.401
IPC_peptide 3.605
IPC2_peptide 3.719
IPC2.peptide.svr19 3.744
Protein with the highest isoelectric point:
>tr|G8UKB5|G8UKB5_TANFA Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase OS=Tannerella forsythia (strain ATCC 43037 / JCM 10827 / CCUG 33226 / KCTC 5666 / FDC 338) OX=203275 GN=BFO_1375 PE=4 SV=1
MM1 pKa = 7.79 RR2 pKa = 11.84 PMIKK6 pKa = 8.66 KK7 pKa = 8.31 TKK9 pKa = 8.39 RR10 pKa = 11.84 QQLGRR15 pKa = 11.84 VLNIALNVFNGISMIIIGFLLMRR38 pKa = 11.84 VFVFGSS44 pKa = 3.28
Molecular weight: 5.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 10.76
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.281
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 12.047
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.065
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2978
0
2978
978559
37
3058
328.6
37.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.098 ± 0.044
1.2 ± 0.019
5.418 ± 0.03
6.416 ± 0.045
4.666 ± 0.035
6.837 ± 0.044
2.086 ± 0.022
6.742 ± 0.039
6.282 ± 0.045
9.093 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.752 ± 0.022
4.693 ± 0.039
3.888 ± 0.027
3.348 ± 0.027
5.563 ± 0.038
6.107 ± 0.039
5.775 ± 0.044
6.394 ± 0.037
1.234 ± 0.017
4.408 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here