Simian immunodeficiency virus (isolate EK505) (SIV-cpz) (Chimpanzee immunodeficiency virus)
Average proteome isoelectric point is 7.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q1A245|REV_SIVEK Protein Rev OS=Simian immunodeficiency virus (isolate EK505) OX=388912 GN=rev PE=3 SV=1
MM1 pKa = 7.62 LLLIKK6 pKa = 10.64 LGFIGLAIEE15 pKa = 4.03 TLIVIVVWAIVYY27 pKa = 9.82 RR28 pKa = 11.84 IYY30 pKa = 11.02 RR31 pKa = 11.84 EE32 pKa = 3.96 VKK34 pKa = 8.82 VEE36 pKa = 4.0 EE37 pKa = 5.16 KK38 pKa = 10.32 ISQLRR43 pKa = 11.84 QRR45 pKa = 11.84 IRR47 pKa = 11.84 DD48 pKa = 3.56 RR49 pKa = 11.84 AEE51 pKa = 4.07 DD52 pKa = 3.62 SGNEE56 pKa = 3.89 SDD58 pKa = 5.71 GDD60 pKa = 4.02 AEE62 pKa = 4.16 EE63 pKa = 4.73 LANLLPPDD71 pKa = 5.9 RR72 pKa = 11.84 IDD74 pKa = 3.21 QDD76 pKa = 3.08 NWVV79 pKa = 3.52
Molecular weight: 9.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.747
IPC2_protein 4.647
IPC_protein 4.495
Toseland 4.329
ProMoST 4.596
Dawson 4.444
Bjellqvist 4.596
Wikipedia 4.317
Rodwell 4.329
Grimsley 4.24
Solomon 4.431
Lehninger 4.38
Nozaki 4.546
DTASelect 4.698
Thurlkill 4.342
EMBOSS 4.329
Sillero 4.596
Patrickios 4.304
IPC_peptide 4.431
IPC2_peptide 4.584
IPC2.peptide.svr19 4.562
Protein with the highest isoelectric point:
>sp|Q1A246|TAT_SIVEK Protein Tat OS=Simian immunodeficiency virus (isolate EK505) OX=388912 GN=tat PE=3 SV=1
MM1 pKa = 7.78 AGRR4 pKa = 11.84 SGVNDD9 pKa = 3.64 EE10 pKa = 4.36 EE11 pKa = 4.38 LLRR14 pKa = 11.84 AIRR17 pKa = 11.84 VIKK20 pKa = 10.18 ILYY23 pKa = 8.97 QSNPYY28 pKa = 9.86 PNDD31 pKa = 3.19 SGTRR35 pKa = 11.84 QARR38 pKa = 11.84 KK39 pKa = 8.34 NRR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 WRR46 pKa = 11.84 ARR48 pKa = 11.84 QRR50 pKa = 11.84 QIRR53 pKa = 11.84 ALSEE57 pKa = 4.76 RR58 pKa = 11.84 ILSSCVGGLQEE69 pKa = 5.31 PSTLPLPPLDD79 pKa = 3.83 RR80 pKa = 11.84 LSLNPEE86 pKa = 3.77 EE87 pKa = 5.36 DD88 pKa = 3.41 LGTSEE93 pKa = 4.65 TEE95 pKa = 4.26 HH96 pKa = 6.28 PQGTATTT103 pKa = 4.06
Molecular weight: 11.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.336
IPC2_protein 9.37
IPC_protein 10.438
Toseland 10.394
ProMoST 10.262
Dawson 10.54
Bjellqvist 10.335
Wikipedia 10.818
Rodwell 10.496
Grimsley 10.613
Solomon 10.701
Lehninger 10.657
Nozaki 10.409
DTASelect 10.321
Thurlkill 10.423
EMBOSS 10.818
Sillero 10.482
Patrickios 10.292
IPC_peptide 10.701
IPC2_peptide 9.531
IPC2.peptide.svr19 8.581
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
3562
79
1448
395.8
44.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.345 ± 0.698
1.965 ± 0.337
3.734 ± 0.364
7.103 ± 0.752
2.442 ± 0.255
7.383 ± 0.228
2.021 ± 0.462
6.541 ± 1.007
6.429 ± 1.015
8.703 ± 0.611
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.302 ± 0.306
5.053 ± 0.766
5.503 ± 0.545
6.176 ± 0.167
5.924 ± 0.824
4.66 ± 0.473
6.092 ± 0.555
6.12 ± 0.418
2.695 ± 0.281
2.807 ± 0.22
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here