Amino acid dipepetide frequency for Eubacterium saphenum ATCC 49989

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.323AlaAla: 6.323 ± 0.213
0.996AlaCys: 0.996 ± 0.07
4.43AlaAsp: 4.43 ± 0.127
4.844AlaGlu: 4.844 ± 0.127
3.023AlaPhe: 3.023 ± 0.104
6.068AlaGly: 6.068 ± 0.127
1.075AlaHis: 1.075 ± 0.063
5.882AlaIle: 5.882 ± 0.16
6.783AlaLys: 6.783 ± 0.193
6.789AlaLeu: 6.789 ± 0.168
2.308AlaMet: 2.308 ± 0.086
3.395AlaAsn: 3.395 ± 0.125
2.009AlaPro: 2.009 ± 0.104
1.534AlaGln: 1.534 ± 0.062
3.051AlaArg: 3.051 ± 0.105
4.448AlaSer: 4.448 ± 0.13
3.632AlaThr: 3.632 ± 0.125
6.037AlaVal: 6.037 ± 0.166
0.438AlaTrp: 0.438 ± 0.034
2.512AlaTyr: 2.512 ± 0.096
0.0AlaXaa: 0.0 ± 0.0
Cys
0.764CysAla: 0.764 ± 0.047
0.183CysCys: 0.183 ± 0.021
0.722CysAsp: 0.722 ± 0.05
0.84CysGlu: 0.84 ± 0.053
0.481CysPhe: 0.481 ± 0.038
1.093CysGly: 1.093 ± 0.062
0.149CysHis: 0.149 ± 0.022
1.114CysIle: 1.114 ± 0.063
1.023CysLys: 1.023 ± 0.071
0.734CysLeu: 0.734 ± 0.052
0.359CysMet: 0.359 ± 0.033
0.466CysAsn: 0.466 ± 0.039
0.374CysPro: 0.374 ± 0.034
0.167CysGln: 0.167 ± 0.027
0.448CysArg: 0.448 ± 0.043
0.709CysSer: 0.709 ± 0.052
0.588CysThr: 0.588 ± 0.046
0.609CysVal: 0.609 ± 0.046
0.067CysTrp: 0.067 ± 0.016
0.356CysTyr: 0.356 ± 0.032
0.0CysXaa: 0.0 ± 0.0
Asp
4.299AspAla: 4.299 ± 0.113
0.53AspCys: 0.53 ± 0.046
3.419AspAsp: 3.419 ± 0.114
5.681AspGlu: 5.681 ± 0.175
2.831AspPhe: 2.831 ± 0.098
4.125AspGly: 4.125 ± 0.14
0.722AspHis: 0.722 ± 0.048
5.964AspIle: 5.964 ± 0.17
6.049AspLys: 6.049 ± 0.189
4.554AspLeu: 4.554 ± 0.14
2.028AspMet: 2.028 ± 0.102
2.557AspAsn: 2.557 ± 0.096
1.671AspPro: 1.671 ± 0.082
0.81AspGln: 0.81 ± 0.056
2.512AspArg: 2.512 ± 0.091
3.188AspSer: 3.188 ± 0.111
2.81AspThr: 2.81 ± 0.124
5.157AspVal: 5.157 ± 0.145
0.307AspTrp: 0.307 ± 0.031
2.341AspTyr: 2.341 ± 0.093
0.0AspXaa: 0.0 ± 0.0
Glu
5.507GluAla: 5.507 ± 0.153
0.703GluCys: 0.703 ± 0.051
4.497GluAsp: 4.497 ± 0.141
5.523GluGlu: 5.523 ± 0.204
2.95GluPhe: 2.95 ± 0.099
4.932GluGly: 4.932 ± 0.127
1.099GluHis: 1.099 ± 0.067
5.915GluIle: 5.915 ± 0.145
7.197GluLys: 7.197 ± 0.162
6.275GluLeu: 6.275 ± 0.179
1.976GluMet: 1.976 ± 0.075
4.21GluAsn: 4.21 ± 0.14
1.65GluPro: 1.65 ± 0.077
1.586GluGln: 1.586 ± 0.066
3.032GluArg: 3.032 ± 0.096
3.276GluSer: 3.276 ± 0.116
2.585GluThr: 2.585 ± 0.089
4.962GluVal: 4.962 ± 0.136
0.353GluTrp: 0.353 ± 0.029
2.92GluTyr: 2.92 ± 0.105
0.0GluXaa: 0.0 ± 0.0
Phe
3.014PheAla: 3.014 ± 0.115
0.469PheCys: 0.469 ± 0.041
2.847PheAsp: 2.847 ± 0.117
2.755PheGlu: 2.755 ± 0.089
1.863PhePhe: 1.863 ± 0.084
2.621PheGly: 2.621 ± 0.112
0.56PheHis: 0.56 ± 0.04
3.361PheIle: 3.361 ± 0.145
3.982PheLys: 3.982 ± 0.13
3.55PheLeu: 3.55 ± 0.126
1.196PheMet: 1.196 ± 0.073
1.936PheAsn: 1.936 ± 0.074
1.136PhePro: 1.136 ± 0.072
0.664PheGln: 0.664 ± 0.047
1.702PheArg: 1.702 ± 0.078
3.084PheSer: 3.084 ± 0.107
2.417PheThr: 2.417 ± 0.103
2.536PheVal: 2.536 ± 0.108
0.244PheTrp: 0.244 ± 0.029
1.382PheTyr: 1.382 ± 0.075
0.0PheXaa: 0.0 ± 0.0
Gly
5.504GlyAla: 5.504 ± 0.156
1.032GlyCys: 1.032 ± 0.065
4.293GlyAsp: 4.293 ± 0.111
4.676GlyGlu: 4.676 ± 0.17
3.312GlyPhe: 3.312 ± 0.12
5.002GlyGly: 5.002 ± 0.174
1.187GlyHis: 1.187 ± 0.051
6.804GlyIle: 6.804 ± 0.17
6.734GlyLys: 6.734 ± 0.173
5.672GlyLeu: 5.672 ± 0.183
2.149GlyMet: 2.149 ± 0.091
3.239GlyAsn: 3.239 ± 0.126
1.099GlyPro: 1.099 ± 0.064
1.467GlyGln: 1.467 ± 0.066
3.264GlyArg: 3.264 ± 0.113
4.649GlySer: 4.649 ± 0.107
3.385GlyThr: 3.385 ± 0.105
4.618GlyVal: 4.618 ± 0.113
0.627GlyTrp: 0.627 ± 0.08
2.932GlyTyr: 2.932 ± 0.128
0.0GlyXaa: 0.0 ± 0.0
His
1.014HisAla: 1.014 ± 0.061
0.183HisCys: 0.183 ± 0.023
0.746HisAsp: 0.746 ± 0.05
1.196HisGlu: 1.196 ± 0.058
0.697HisPhe: 0.697 ± 0.049
1.206HisGly: 1.206 ± 0.073
0.28HisHis: 0.28 ± 0.033
1.352HisIle: 1.352 ± 0.067
1.187HisLys: 1.187 ± 0.071
1.175HisLeu: 1.175 ± 0.07
0.429HisMet: 0.429 ± 0.038
0.661HisAsn: 0.661 ± 0.039
0.7HisPro: 0.7 ± 0.047
0.32HisGln: 0.32 ± 0.029
0.758HisArg: 0.758 ± 0.054
1.014HisSer: 1.014 ± 0.056
0.77HisThr: 0.77 ± 0.045
0.919HisVal: 0.919 ± 0.052
0.113HisTrp: 0.113 ± 0.02
0.527HisTyr: 0.527 ± 0.04
0.0HisXaa: 0.0 ± 0.0
Ile
6.686IleAla: 6.686 ± 0.205
0.996IleCys: 0.996 ± 0.064
5.489IleAsp: 5.489 ± 0.139
6.165IleGlu: 6.165 ± 0.161
3.188IlePhe: 3.188 ± 0.111
5.41IleGly: 5.41 ± 0.165
1.157IleHis: 1.157 ± 0.069
6.241IleIle: 6.241 ± 0.212
7.456IleLys: 7.456 ± 0.147
6.771IleLeu: 6.771 ± 0.225
2.015IleMet: 2.015 ± 0.096
4.067IleAsn: 4.067 ± 0.103
2.758IlePro: 2.758 ± 0.106
1.51IleGln: 1.51 ± 0.075
3.309IleArg: 3.309 ± 0.099
5.69IleSer: 5.69 ± 0.159
4.564IleThr: 4.564 ± 0.13
5.516IleVal: 5.516 ± 0.153
0.368IleTrp: 0.368 ± 0.039
2.655IleTyr: 2.655 ± 0.088
0.0IleXaa: 0.0 ± 0.0
Lys
6.737LysAla: 6.737 ± 0.181
0.731LysCys: 0.731 ± 0.057
5.669LysAsp: 5.669 ± 0.16
7.084LysGlu: 7.084 ± 0.187
3.154LysPhe: 3.154 ± 0.093
6.034LysGly: 6.034 ± 0.172
1.364LysHis: 1.364 ± 0.064
7.16LysIle: 7.16 ± 0.177
9.922LysLys: 9.922 ± 0.246
7.934LysLeu: 7.934 ± 0.164
2.658LysMet: 2.658 ± 0.09
5.538LysAsn: 5.538 ± 0.155
2.585LysPro: 2.585 ± 0.111
2.369LysGln: 2.369 ± 0.087
4.372LysArg: 4.372 ± 0.125
5.291LysSer: 5.291 ± 0.124
4.667LysThr: 4.667 ± 0.132
6.247LysVal: 6.247 ± 0.204
0.929LysTrp: 0.929 ± 0.1
3.452LysTyr: 3.452 ± 0.125
0.0LysXaa: 0.0 ± 0.0
Leu
6.661LeuAla: 6.661 ± 0.171
0.986LeuCys: 0.986 ± 0.063
4.99LeuAsp: 4.99 ± 0.147
5.468LeuGlu: 5.468 ± 0.158
3.218LeuPhe: 3.218 ± 0.122
6.095LeuGly: 6.095 ± 0.15
1.248LeuHis: 1.248 ± 0.064
6.299LeuIle: 6.299 ± 0.21
8.116LeuLys: 8.116 ± 0.168
7.011LeuLeu: 7.011 ± 0.224
2.329LeuMet: 2.329 ± 0.096
4.146LeuAsn: 4.146 ± 0.139
3.117LeuPro: 3.117 ± 0.105
1.79LeuGln: 1.79 ± 0.08
3.425LeuArg: 3.425 ± 0.126
6.278LeuSer: 6.278 ± 0.173
4.058LeuThr: 4.058 ± 0.115
5.523LeuVal: 5.523 ± 0.164
0.511LeuTrp: 0.511 ± 0.045
2.673LeuTyr: 2.673 ± 0.106
0.0LeuXaa: 0.0 ± 0.0
Met
2.104MetAla: 2.104 ± 0.089
0.28MetCys: 0.28 ± 0.028
1.799MetAsp: 1.799 ± 0.067
1.61MetGlu: 1.61 ± 0.069
1.403MetPhe: 1.403 ± 0.142
2.012MetGly: 2.012 ± 0.078
0.502MetHis: 0.502 ± 0.047
1.903MetIle: 1.903 ± 0.086
2.716MetLys: 2.716 ± 0.084
2.624MetLeu: 2.624 ± 0.114
0.767MetMet: 0.767 ± 0.052
1.41MetAsn: 1.41 ± 0.072
1.19MetPro: 1.19 ± 0.06
1.059MetGln: 1.059 ± 0.053
1.203MetArg: 1.203 ± 0.073
1.9MetSer: 1.9 ± 0.07
1.306MetThr: 1.306 ± 0.073
1.784MetVal: 1.784 ± 0.074
0.174MetTrp: 0.174 ± 0.022
0.804MetTyr: 0.804 ± 0.047
0.0MetXaa: 0.0 ± 0.0
Asn
3.532AsnAla: 3.532 ± 0.109
0.441AsnCys: 0.441 ± 0.036
2.706AsnAsp: 2.706 ± 0.099
3.538AsnGlu: 3.538 ± 0.102
1.839AsnPhe: 1.839 ± 0.089
3.312AsnGly: 3.312 ± 0.108
0.697AsnHis: 0.697 ± 0.047
4.624AsnIle: 4.624 ± 0.126
4.323AsnLys: 4.323 ± 0.139
4.058AsnLeu: 4.058 ± 0.124
1.586AsnMet: 1.586 ± 0.096
2.085AsnAsn: 2.085 ± 0.102
2.037AsnPro: 2.037 ± 0.1
0.926AsnGln: 0.926 ± 0.065
1.674AsnArg: 1.674 ± 0.065
2.773AsnSer: 2.773 ± 0.087
2.624AsnThr: 2.624 ± 0.1
3.617AsnVal: 3.617 ± 0.146
0.438AsnTrp: 0.438 ± 0.058
1.592AsnTyr: 1.592 ± 0.068
0.0AsnXaa: 0.0 ± 0.0
Pro
2.253ProAla: 2.253 ± 0.098
0.365ProCys: 0.365 ± 0.033
2.146ProAsp: 2.146 ± 0.12
2.557ProGlu: 2.557 ± 0.103
1.236ProPhe: 1.236 ± 0.07
1.982ProGly: 1.982 ± 0.101
0.521ProHis: 0.521 ± 0.038
2.146ProIle: 2.146 ± 0.094
2.569ProLys: 2.569 ± 0.108
2.454ProLeu: 2.454 ± 0.09
0.764ProMet: 0.764 ± 0.052
1.27ProAsn: 1.27 ± 0.072
0.837ProPro: 0.837 ± 0.064
0.822ProGln: 0.822 ± 0.053
0.944ProArg: 0.944 ± 0.055
1.906ProSer: 1.906 ± 0.078
1.492ProThr: 1.492 ± 0.068
2.487ProVal: 2.487 ± 0.086
0.18ProTrp: 0.18 ± 0.028
1.133ProTyr: 1.133 ± 0.066
0.0ProXaa: 0.0 ± 0.0
Gln
1.562GlnAla: 1.562 ± 0.069
0.167GlnCys: 0.167 ± 0.027
1.172GlnAsp: 1.172 ± 0.06
1.4GlnGlu: 1.4 ± 0.077
0.782GlnPhe: 0.782 ± 0.048
1.824GlnGly: 1.824 ± 0.074
0.295GlnHis: 0.295 ± 0.031
1.775GlnIle: 1.775 ± 0.09
2.107GlnLys: 2.107 ± 0.085
1.735GlnLeu: 1.735 ± 0.071
0.697GlnMet: 0.697 ± 0.041
1.318GlnAsn: 1.318 ± 0.063
0.502GlnPro: 0.502 ± 0.037
0.545GlnGln: 0.545 ± 0.045
1.087GlnArg: 1.087 ± 0.061
1.303GlnSer: 1.303 ± 0.066
1.047GlnThr: 1.047 ± 0.057
1.4GlnVal: 1.4 ± 0.064
0.134GlnTrp: 0.134 ± 0.022
0.655GlnTyr: 0.655 ± 0.046
0.0GlnXaa: 0.0 ± 0.0
Arg
3.008ArgAla: 3.008 ± 0.096
0.472ArgCys: 0.472 ± 0.04
2.573ArgAsp: 2.573 ± 0.102
3.242ArgGlu: 3.242 ± 0.115
1.717ArgPhe: 1.717 ± 0.079
2.974ArgGly: 2.974 ± 0.097
0.767ArgHis: 0.767 ± 0.057
3.592ArgIle: 3.592 ± 0.107
3.681ArgLys: 3.681 ± 0.11
3.696ArgLeu: 3.696 ± 0.109
1.206ArgMet: 1.206 ± 0.061
2.125ArgAsn: 2.125 ± 0.083
1.114ArgPro: 1.114 ± 0.054
1.126ArgGln: 1.126 ± 0.058
2.143ArgArg: 2.143 ± 0.101
2.158ArgSer: 2.158 ± 0.078
1.647ArgThr: 1.647 ± 0.061
2.499ArgVal: 2.499 ± 0.09
0.323ArgTrp: 0.323 ± 0.036
1.687ArgTyr: 1.687 ± 0.07
0.0ArgXaa: 0.0 ± 0.0
Ser
4.688SerAla: 4.688 ± 0.184
0.725SerCys: 0.725 ± 0.05
3.848SerAsp: 3.848 ± 0.109
4.381SerGlu: 4.381 ± 0.131
2.746SerPhe: 2.746 ± 0.109
5.169SerGly: 5.169 ± 0.144
1.02SerHis: 1.02 ± 0.056
5.139SerIle: 5.139 ± 0.133
6.579SerLys: 6.579 ± 0.208
5.133SerLeu: 5.133 ± 0.133
1.674SerMet: 1.674 ± 0.068
2.822SerAsn: 2.822 ± 0.128
1.903SerPro: 1.903 ± 0.087
1.358SerGln: 1.358 ± 0.065
2.369SerArg: 2.369 ± 0.087
3.863SerSer: 3.863 ± 0.122
2.807SerThr: 2.807 ± 0.108
3.754SerVal: 3.754 ± 0.099
0.39SerTrp: 0.39 ± 0.036
2.021SerTyr: 2.021 ± 0.081
0.0SerXaa: 0.0 ± 0.0
Thr
4.107ThrAla: 4.107 ± 0.137
0.585ThrCys: 0.585 ± 0.039
3.002ThrAsp: 3.002 ± 0.115
3.096ThrGlu: 3.096 ± 0.105
2.113ThrPhe: 2.113 ± 0.096
4.393ThrGly: 4.393 ± 0.117
0.782ThrHis: 0.782 ± 0.051
3.644ThrIle: 3.644 ± 0.121
3.659ThrLys: 3.659 ± 0.131
4.357ThrLeu: 4.357 ± 0.135
1.154ThrMet: 1.154 ± 0.061
1.9ThrAsn: 1.9 ± 0.087
1.912ThrPro: 1.912 ± 0.078
1.12ThrGln: 1.12 ± 0.066
1.747ThrArg: 1.747 ± 0.069
3.148ThrSer: 3.148 ± 0.157
2.359ThrThr: 2.359 ± 0.091
3.626ThrVal: 3.626 ± 0.124
0.289ThrTrp: 0.289 ± 0.032
1.659ThrTyr: 1.659 ± 0.074
0.0ThrXaa: 0.0 ± 0.0
Val
4.895ValAla: 4.895 ± 0.131
0.944ValCys: 0.944 ± 0.061
4.229ValAsp: 4.229 ± 0.122
3.912ValGlu: 3.912 ± 0.142
3.035ValPhe: 3.035 ± 0.115
4.436ValGly: 4.436 ± 0.124
1.02ValHis: 1.02 ± 0.056
5.8ValIle: 5.8 ± 0.153
6.019ValLys: 6.019 ± 0.153
6.025ValLeu: 6.025 ± 0.17
1.973ValMet: 1.973 ± 0.09
3.218ValAsn: 3.218 ± 0.092
2.183ValPro: 2.183 ± 0.084
1.434ValGln: 1.434 ± 0.058
2.731ValArg: 2.731 ± 0.096
4.972ValSer: 4.972 ± 0.161
3.991ValThr: 3.991 ± 0.187
4.978ValVal: 4.978 ± 0.136
0.484ValTrp: 0.484 ± 0.04
2.627ValTyr: 2.627 ± 0.09
0.0ValXaa: 0.0 ± 0.0
Trp
0.454TrpAla: 0.454 ± 0.037
0.088TrpCys: 0.088 ± 0.018
0.542TrpAsp: 0.542 ± 0.068
0.408TrpGlu: 0.408 ± 0.039
0.265TrpPhe: 0.265 ± 0.032
0.426TrpGly: 0.426 ± 0.033
0.17TrpHis: 0.17 ± 0.024
0.499TrpIle: 0.499 ± 0.039
0.67TrpLys: 0.67 ± 0.069
0.432TrpLeu: 0.432 ± 0.037
0.222TrpMet: 0.222 ± 0.027
0.441TrpAsn: 0.441 ± 0.059
0.119TrpPro: 0.119 ± 0.021
0.253TrpGln: 0.253 ± 0.027
0.301TrpArg: 0.301 ± 0.034
0.411TrpSer: 0.411 ± 0.065
0.213TrpThr: 0.213 ± 0.028
0.432TrpVal: 0.432 ± 0.041
0.064TrpTrp: 0.064 ± 0.014
0.222TrpTyr: 0.222 ± 0.027
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.463TyrAla: 2.463 ± 0.142
0.371TyrCys: 0.371 ± 0.033
2.439TyrAsp: 2.439 ± 0.09
2.77TyrGlu: 2.77 ± 0.091
1.464TyrPhe: 1.464 ± 0.076
2.533TyrGly: 2.533 ± 0.082
0.63TyrHis: 0.63 ± 0.048
2.795TyrIle: 2.795 ± 0.119
3.124TyrLys: 3.124 ± 0.099
2.968TyrLeu: 2.968 ± 0.104
1.056TyrMet: 1.056 ± 0.069
1.62TyrAsn: 1.62 ± 0.078
1.166TyrPro: 1.166 ± 0.068
0.648TyrGln: 0.648 ± 0.048
1.681TyrArg: 1.681 ± 0.066
2.329TyrSer: 2.329 ± 0.085
1.69TyrThr: 1.69 ± 0.077
2.201TyrVal: 2.201 ± 0.081
0.201TyrTrp: 0.201 ± 0.021
1.385TyrTyr: 1.385 ± 0.069
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 952 proteins (328471 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski