Lake Sarah-associated circular virus-51

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126GA35|A0A126GA35_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-51 OX=1685781 PE=3 SV=1
MM1 pKa = 7.25QNTHH5 pKa = 6.46ASSDD9 pKa = 3.91EE10 pKa = 4.05EE11 pKa = 4.63VEE13 pKa = 4.3AGPSRR18 pKa = 11.84TFRR21 pKa = 11.84IDD23 pKa = 3.11ARR25 pKa = 11.84HH26 pKa = 6.5LEE28 pKa = 4.28LTYY31 pKa = 10.73SRR33 pKa = 11.84CNATVEE39 pKa = 4.86DD40 pKa = 3.5ITEE43 pKa = 4.05FLEE46 pKa = 4.3TTVASSAQCHH56 pKa = 4.98SWFAARR62 pKa = 11.84EE63 pKa = 3.92YY64 pKa = 10.52HH65 pKa = 6.49QDD67 pKa = 3.37GVPHH71 pKa = 4.9YY72 pKa = 10.37HH73 pKa = 6.96ILVKK77 pKa = 10.35FNKK80 pKa = 9.5RR81 pKa = 11.84RR82 pKa = 11.84TFRR85 pKa = 11.84NSRR88 pKa = 11.84EE89 pKa = 3.6YY90 pKa = 11.04DD91 pKa = 3.59FNGHH95 pKa = 6.04HH96 pKa = 6.83PRR98 pKa = 11.84IKK100 pKa = 10.04PVRR103 pKa = 11.84NVHH106 pKa = 7.08DD107 pKa = 3.87YY108 pKa = 10.6FRR110 pKa = 11.84YY111 pKa = 9.96ISDD114 pKa = 3.44PSKK117 pKa = 10.43PSRR120 pKa = 11.84DD121 pKa = 3.22TTFASTGWMDD131 pKa = 5.28PIPRR135 pKa = 11.84DD136 pKa = 3.33GARR139 pKa = 11.84GGGRR143 pKa = 11.84GRR145 pKa = 11.84RR146 pKa = 11.84DD147 pKa = 3.42DD148 pKa = 4.96DD149 pKa = 4.83LGGDD153 pKa = 3.55GCTWGDD159 pKa = 3.36IVRR162 pKa = 11.84ADD164 pKa = 3.61GKK166 pKa = 10.68DD167 pKa = 3.24EE168 pKa = 4.26YY169 pKa = 10.66FTLLKK174 pKa = 10.26LHH176 pKa = 6.83KK177 pKa = 10.38PRR179 pKa = 11.84DD180 pKa = 3.68YY181 pKa = 11.22CLNLQRR187 pKa = 11.84LEE189 pKa = 4.2YY190 pKa = 8.89TASRR194 pKa = 11.84LFTAITPYY202 pKa = 10.19VPEE205 pKa = 4.04YY206 pKa = 10.68EE207 pKa = 4.03YY208 pKa = 9.98DD209 pKa = 3.5TFNVPEE215 pKa = 4.74EE216 pKa = 3.94LHH218 pKa = 5.97EE219 pKa = 4.03WATVGRR225 pKa = 11.84HH226 pKa = 4.81LQPRR230 pKa = 11.84PKK232 pKa = 10.45SLIICGPSRR241 pKa = 11.84IGKK244 pKa = 6.69TEE246 pKa = 3.64WARR249 pKa = 11.84SIGLHH254 pKa = 5.32TYY256 pKa = 9.15WYY258 pKa = 8.33GQCNIDD264 pKa = 3.27SWNDD268 pKa = 2.85KK269 pKa = 10.11MEE271 pKa = 4.11YY272 pKa = 10.51LIMDD276 pKa = 4.92DD277 pKa = 5.07FSPEE281 pKa = 3.4ITKK284 pKa = 9.85YY285 pKa = 10.16LPLWKK290 pKa = 10.19GYY292 pKa = 10.49FGAQKK297 pKa = 10.09EE298 pKa = 4.36LNVTQKK304 pKa = 10.62YY305 pKa = 9.78RR306 pKa = 11.84GIYY309 pKa = 7.96TKK311 pKa = 10.71LFRR314 pKa = 11.84VPVLWLCNQIPILKK328 pKa = 9.96DD329 pKa = 3.28WEE331 pKa = 4.32QEE333 pKa = 4.09WLNMNADD340 pKa = 3.85TYY342 pKa = 10.87FINDD346 pKa = 3.45KK347 pKa = 11.11LYY349 pKa = 10.79GG350 pKa = 3.74

Molecular weight: 41.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126GA35|A0A126GA35_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-51 OX=1685781 PE=3 SV=1
MM1 pKa = 7.62PPYY4 pKa = 10.26APRR7 pKa = 11.84YY8 pKa = 7.54RR9 pKa = 11.84ASKK12 pKa = 8.87RR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84VGRR19 pKa = 11.84KK20 pKa = 7.45FRR22 pKa = 11.84GRR24 pKa = 11.84KK25 pKa = 5.2RR26 pKa = 11.84TRR28 pKa = 11.84RR29 pKa = 11.84GRR31 pKa = 11.84GGQRR35 pKa = 11.84SVQRR39 pKa = 11.84YY40 pKa = 8.13SNLVRR45 pKa = 11.84LIKK48 pKa = 10.39KK49 pKa = 8.53VAIRR53 pKa = 11.84SAEE56 pKa = 4.07SKK58 pKa = 10.69FNYY61 pKa = 10.2LYY63 pKa = 10.94GSQLTALAGQGYY75 pKa = 10.5LMNPWMGITEE85 pKa = 4.3GTSQEE90 pKa = 3.81QRR92 pKa = 11.84VGNEE96 pKa = 3.94VYY98 pKa = 10.88VKK100 pKa = 10.97GMLLKK105 pKa = 11.16GNITCSANYY114 pKa = 9.51PIDD117 pKa = 3.89DD118 pKa = 3.58RR119 pKa = 11.84TVRR122 pKa = 11.84ISLIWCPDD130 pKa = 3.04NLVDD134 pKa = 4.05HH135 pKa = 6.08THH137 pKa = 5.33VSAFVRR143 pKa = 11.84YY144 pKa = 10.19DD145 pKa = 3.55NIVDD149 pKa = 3.79NEE151 pKa = 4.19GNLYY155 pKa = 9.48IDD157 pKa = 2.95NWYY160 pKa = 10.56QYY162 pKa = 10.71GVLPNSIGYY171 pKa = 10.6ANASFDD177 pKa = 4.05KK178 pKa = 10.92DD179 pKa = 3.19SGIAVLYY186 pKa = 8.62QKK188 pKa = 10.43RR189 pKa = 11.84IKK191 pKa = 10.3LRR193 pKa = 11.84QSHH196 pKa = 5.01NTWRR200 pKa = 11.84SDD202 pKa = 3.17TNLAGVSLHH211 pKa = 7.44PIRR214 pKa = 11.84CWVPMKK220 pKa = 10.36LRR222 pKa = 11.84KK223 pKa = 9.25FKK225 pKa = 10.9FVGDD229 pKa = 4.18GGNDD233 pKa = 3.24VYY235 pKa = 10.87RR236 pKa = 11.84GSKK239 pKa = 8.33GQYY242 pKa = 7.77YY243 pKa = 9.57WLFDD247 pKa = 3.46VTDD250 pKa = 3.58TFGTVASTPMLAFDD264 pKa = 5.61WISKK268 pKa = 10.25VYY270 pKa = 10.49FKK272 pKa = 11.21DD273 pKa = 3.31PP274 pKa = 3.49

Molecular weight: 31.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

624

274

350

312.0

36.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.968 ± 0.082

1.603 ± 0.293

6.731 ± 0.514

4.327 ± 1.443

4.006 ± 0.206

7.532 ± 0.708

2.724 ± 0.729

5.288 ± 0.103

5.449 ± 0.436

7.212 ± 0.16

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.763 ± 0.246

4.968 ± 0.503

4.808 ± 0.457

3.205 ± 0.256

8.814 ± 0.6

5.929 ± 0.58

5.929 ± 0.684

5.609 ± 1.186

2.885 ± 0.19

6.25 ± 0.184
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski