Jatropha mosaic India virus-[Lucknow]
Average proteome isoelectric point is 8.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E2FZU7|E2FZU7_9GEMI Capsid protein OS=Jatropha mosaic India virus-[Lucknow] OX=569949 GN=AV1 PE=3 SV=1
MM1 pKa = 8.06 WDD3 pKa = 3.35 PLLNEE8 pKa = 4.99 FPDD11 pKa = 3.86 SVHH14 pKa = 6.81 GFRR17 pKa = 11.84 CMLALKK23 pKa = 9.76 YY24 pKa = 10.16 LQLVEE29 pKa = 4.41 STYY32 pKa = 11.41 SPDD35 pKa = 3.14 TLGYY39 pKa = 10.84 DD40 pKa = 4.83 LIRR43 pKa = 11.84 DD44 pKa = 4.71 LISVIRR50 pKa = 11.84 ARR52 pKa = 11.84 SYY54 pKa = 10.28 VEE56 pKa = 3.27 ATRR59 pKa = 11.84 RR60 pKa = 11.84 YY61 pKa = 8.39 HH62 pKa = 6.31 HH63 pKa = 6.62 FNSRR67 pKa = 11.84 LEE69 pKa = 4.21 GTSPSDD75 pKa = 3.47 LRR77 pKa = 11.84 QLIQQPCFCLHH88 pKa = 6.9 CPRR91 pKa = 11.84 HH92 pKa = 5.61 QKK94 pKa = 9.39 TSLDD98 pKa = 3.79 KK99 pKa = 10.61 QAHH102 pKa = 5.06 EE103 pKa = 5.14 SEE105 pKa = 4.26 AQMVQDD111 pKa = 3.71 VQKK114 pKa = 10.17 PRR116 pKa = 11.84 CSS118 pKa = 3.2
Molecular weight: 13.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.507
IPC2_protein 6.44
IPC_protein 6.649
Toseland 6.664
ProMoST 6.927
Dawson 7.146
Bjellqvist 7.0
Wikipedia 7.117
Rodwell 7.132
Grimsley 6.751
Solomon 7.19
Lehninger 7.205
Nozaki 7.366
DTASelect 7.439
Thurlkill 7.468
EMBOSS 7.497
Sillero 7.571
Patrickios 3.897
IPC_peptide 7.205
IPC2_peptide 7.293
IPC2.peptide.svr19 7.129
Protein with the highest isoelectric point:
>tr|E2FZU8|E2FZU8_9GEMI Replication enhancer OS=Jatropha mosaic India virus-[Lucknow] OX=569949 GN=AC3 PE=3 SV=1
MM1 pKa = 7.7 SKK3 pKa = 10.44 RR4 pKa = 11.84 PGDD7 pKa = 3.89 IIISTPASKK16 pKa = 10.31 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LIFDD24 pKa = 3.31 SLYY27 pKa = 10.61 SSRR30 pKa = 11.84 ASVFTVRR37 pKa = 11.84 VTKK40 pKa = 10.46 RR41 pKa = 11.84 QAWTNRR47 pKa = 11.84 PMNRR51 pKa = 11.84 KK52 pKa = 7.93 PRR54 pKa = 11.84 WYY56 pKa = 10.73 RR57 pKa = 11.84 MFRR60 pKa = 11.84 SPDD63 pKa = 3.23 VPRR66 pKa = 11.84 GCEE69 pKa = 4.34 GPCKK73 pKa = 10.01 VQSFEE78 pKa = 4.02 SRR80 pKa = 11.84 HH81 pKa = 5.54 DD82 pKa = 3.51 VVHH85 pKa = 6.75 IGKK88 pKa = 9.41 VMCISDD94 pKa = 3.53 VTRR97 pKa = 11.84 GVGLTHH103 pKa = 7.14 RR104 pKa = 11.84 VGKK107 pKa = 9.66 RR108 pKa = 11.84 FCVKK112 pKa = 9.92 SVYY115 pKa = 10.24 ILGKK119 pKa = 9.73 VWMDD123 pKa = 3.27 EE124 pKa = 4.06 NIKK127 pKa = 9.31 TKK129 pKa = 10.6 NHH131 pKa = 5.78 TNSVMFFLVRR141 pKa = 11.84 DD142 pKa = 3.84 RR143 pKa = 11.84 RR144 pKa = 11.84 PVDD147 pKa = 3.13 KK148 pKa = 10.28 PQDD151 pKa = 3.56 FGEE154 pKa = 4.33 VFNMFDD160 pKa = 4.29 NEE162 pKa = 4.11 PSTATVKK169 pKa = 10.61 NMHH172 pKa = 7.0 RR173 pKa = 11.84 DD174 pKa = 3.31 RR175 pKa = 11.84 YY176 pKa = 9.22 QVLRR180 pKa = 11.84 KK181 pKa = 9.45 WSATVTGGQYY191 pKa = 10.85 ASKK194 pKa = 9.9 EE195 pKa = 3.88 QALVRR200 pKa = 11.84 RR201 pKa = 11.84 FFRR204 pKa = 11.84 VNNYY208 pKa = 7.82 VVYY211 pKa = 9.96 NQQEE215 pKa = 3.8 AGKK218 pKa = 10.1 YY219 pKa = 8.23 EE220 pKa = 4.02 NHH222 pKa = 6.47 TEE224 pKa = 3.99 NALMLYY230 pKa = 7.52 MACTHH235 pKa = 7.06 ASNPVYY241 pKa = 9.86 ATLKK245 pKa = 9.47 IRR247 pKa = 11.84 IYY249 pKa = 10.67 FYY251 pKa = 11.26 DD252 pKa = 3.51 SVSNN256 pKa = 3.9
Molecular weight: 29.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.296
IPC2_protein 9.604
IPC_protein 9.999
Toseland 10.262
ProMoST 9.97
Dawson 10.452
Bjellqvist 10.145
Wikipedia 10.643
Rodwell 10.774
Grimsley 10.526
Solomon 10.482
Lehninger 10.452
Nozaki 10.277
DTASelect 10.131
Thurlkill 10.306
EMBOSS 10.672
Sillero 10.365
Patrickios 10.394
IPC_peptide 10.482
IPC2_peptide 9.063
IPC2.peptide.svr19 8.585
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1090
100
347
181.7
20.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.321 ± 0.524
2.11 ± 0.397
5.413 ± 0.697
4.404 ± 0.514
4.312 ± 0.584
5.321 ± 0.55
3.853 ± 0.71
4.954 ± 0.696
5.413 ± 1.192
7.706 ± 1.192
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.569 ± 0.558
5.229 ± 0.584
5.78 ± 0.62
4.22 ± 0.641
7.431 ± 1.176
8.44 ± 1.067
6.239 ± 0.951
6.055 ± 1.331
1.56 ± 0.201
3.67 ± 0.464
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here