Japanese holly fern mottle virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Mayoviridae; Pteridovirus; Japanese holly fern mottle pteridovirus

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C7T4Z8|C7T4Z8_9VIRU p29 OS=Japanese holly fern mottle virus OX=659660 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 10.1GASGLRR8 pKa = 11.84RR9 pKa = 11.84LGFRR13 pKa = 11.84AVVPAVVVGGAVAGTVVHH31 pKa = 6.84RR32 pKa = 11.84ALNNRR37 pKa = 11.84GGDD40 pKa = 3.89GEE42 pKa = 4.13NLRR45 pKa = 11.84DD46 pKa = 3.86LRR48 pKa = 11.84EE49 pKa = 3.99LRR51 pKa = 11.84PLEE54 pKa = 4.02VEE56 pKa = 4.11EE57 pKa = 4.27FTISSGDD64 pKa = 3.7SVAEE68 pKa = 4.36SIDD71 pKa = 3.4IDD73 pKa = 3.92SAAVNSAADD82 pKa = 4.19LVPFDD87 pKa = 4.82AVLEE91 pKa = 4.19PEE93 pKa = 4.59EE94 pKa = 4.69PVTLPVSNPRR104 pKa = 11.84RR105 pKa = 11.84IWSRR109 pKa = 11.84LFAAMVTLSVPLAGVFAVRR128 pKa = 11.84HH129 pKa = 4.21VTGQGSVSAGDD140 pKa = 3.93DD141 pKa = 3.57LRR143 pKa = 11.84PEE145 pKa = 4.15PFGPRR150 pKa = 11.84DD151 pKa = 3.41VPRR154 pKa = 11.84PDD156 pKa = 4.7EE157 pKa = 4.63IPPHH161 pKa = 5.9AAVEE165 pKa = 4.21EE166 pKa = 4.44GDD168 pKa = 4.01PVACLPEE175 pKa = 4.24DD176 pKa = 2.96SRR178 pKa = 11.84RR179 pKa = 11.84FVKK182 pKa = 10.53RR183 pKa = 11.84CLDD186 pKa = 3.27HH187 pKa = 6.83LAYY190 pKa = 10.43FEE192 pKa = 4.59LVGVCPFCEE201 pKa = 3.81RR202 pKa = 11.84DD203 pKa = 3.51GLKK206 pKa = 10.55SISFEE211 pKa = 3.46AVVFEE216 pKa = 4.28EE217 pKa = 4.55VPPLFVGKK225 pKa = 10.66FEE227 pKa = 6.05LIPSAVFDD235 pKa = 3.58PHH237 pKa = 7.07FWEE240 pKa = 4.42PLCYY244 pKa = 9.09YY245 pKa = 10.31TLFQDD250 pKa = 4.87MLVCKK255 pKa = 10.13CGKK258 pKa = 8.54HH259 pKa = 6.28DD260 pKa = 3.94RR261 pKa = 11.84YY262 pKa = 10.34HH263 pKa = 5.63VVKK266 pKa = 9.79IYY268 pKa = 10.6PVMDD272 pKa = 3.23VHH274 pKa = 6.98PLNTVYY280 pKa = 10.46MLFIVKK286 pKa = 10.04EE287 pKa = 4.13GFDD290 pKa = 3.66VDD292 pKa = 3.58RR293 pKa = 11.84GFPVMSSYY301 pKa = 10.98GYY303 pKa = 8.84HH304 pKa = 6.11VGWAFEE310 pKa = 4.1PLTIPLEE317 pKa = 4.41PFDD320 pKa = 3.98GTEE323 pKa = 4.02PLPLHH328 pKa = 6.56NEE330 pKa = 4.01TGRR333 pKa = 3.54

Molecular weight: 36.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C7T4Z8|C7T4Z8_9VIRU p29 OS=Japanese holly fern mottle virus OX=659660 PE=4 SV=1
MM1 pKa = 7.55APKK4 pKa = 10.14ALPKK8 pKa = 10.31NVPKK12 pKa = 10.11EE13 pKa = 3.79YY14 pKa = 11.12AFIGPKK20 pKa = 9.88LWPKK24 pKa = 9.66LQEE27 pKa = 4.57AISYY31 pKa = 10.36GFDD34 pKa = 2.75IRR36 pKa = 11.84KK37 pKa = 9.68ARR39 pKa = 11.84DD40 pKa = 3.29DD41 pKa = 4.64GMTLSNLPFVEE52 pKa = 4.27KK53 pKa = 10.63EE54 pKa = 3.85KK55 pKa = 11.09VLNAFLKK62 pKa = 10.42SKK64 pKa = 10.12RR65 pKa = 11.84KK66 pKa = 9.65SGSSSSSTTGKK77 pKa = 10.18VPVRR81 pKa = 11.84DD82 pKa = 3.77VPEE85 pKa = 4.05SSVARR90 pKa = 11.84KK91 pKa = 8.08EE92 pKa = 4.08KK93 pKa = 10.02PRR95 pKa = 11.84KK96 pKa = 9.01QKK98 pKa = 8.91PTGVFMWEE106 pKa = 3.8PASLIGVDD114 pKa = 3.69GDD116 pKa = 3.89PGKK119 pKa = 10.41FAALPHH125 pKa = 6.24GKK127 pKa = 9.45SAVLLSFKK135 pKa = 10.56PPASPCMISKK145 pKa = 8.94MLIMLKK151 pKa = 10.14VDD153 pKa = 3.61ATADD157 pKa = 3.73LVVPYY162 pKa = 10.66AGITGIDD169 pKa = 3.45HH170 pKa = 7.21AGDD173 pKa = 3.77LAADD177 pKa = 4.64RR178 pKa = 11.84YY179 pKa = 11.11SVMVVNSRR187 pKa = 11.84YY188 pKa = 8.5PTMKK192 pKa = 10.54LLEE195 pKa = 4.79ADD197 pKa = 4.17DD198 pKa = 4.87KK199 pKa = 11.15ISYY202 pKa = 10.22RR203 pKa = 11.84FTFDD207 pKa = 3.51EE208 pKa = 4.9PVSSDD213 pKa = 3.02LFSRR217 pKa = 11.84IRR219 pKa = 11.84FIVTTANVAKK229 pKa = 9.27TKK231 pKa = 10.31SWLYY235 pKa = 7.68CQCWYY240 pKa = 10.72KK241 pKa = 11.03YY242 pKa = 10.07RR243 pKa = 11.84INKK246 pKa = 8.09TPVEE250 pKa = 3.97KK251 pKa = 10.34GGRR254 pKa = 11.84VMAVRR259 pKa = 11.84FDD261 pKa = 3.58AGAVV265 pKa = 3.35

Molecular weight: 29.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2911

106

1909

582.2

64.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.561 ± 0.428

2.576 ± 0.461

6.699 ± 0.56

5.119 ± 0.517

5.29 ± 0.331

5.29 ± 0.831

2.336 ± 0.51

3.813 ± 0.592

5.496 ± 1.008

9.035 ± 0.592

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.37 ± 0.262

2.92 ± 0.809

5.737 ± 1.231

2.267 ± 0.474

5.806 ± 0.263

9.619 ± 1.008

4.706 ± 0.509

10.271 ± 0.692

1.099 ± 0.165

2.989 ± 0.223
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski