Streptococcus phage Javan47
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6ADW2|A0A4D6ADW2_9CAUD Replicative DNA helicase OS=Streptococcus phage Javan47 OX=2548188 GN=Javan47_0021 PE=4 SV=1
MM1 pKa = 8.02 IEE3 pKa = 4.62 VEE5 pKa = 4.17 RR6 pKa = 11.84 QPKK9 pKa = 7.79 NTYY12 pKa = 10.08 SRR14 pKa = 11.84 IRR16 pKa = 11.84 CSNNIYY22 pKa = 10.88 DD23 pKa = 4.91 EE24 pKa = 4.22 IANIANEE31 pKa = 4.13 CDD33 pKa = 3.44 LTLKK37 pKa = 10.53 EE38 pKa = 4.19 VTDD41 pKa = 3.79 ALLNYY46 pKa = 9.81 GLANAQIISSEE57 pKa = 4.17 KK58 pKa = 10.31 VVTEE62 pKa = 4.05 SKK64 pKa = 10.8 LVIGDD69 pKa = 4.21 FDD71 pKa = 4.23 NDD73 pKa = 3.38 NNNN76 pKa = 3.07
Molecular weight: 8.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.391
IPC2_protein 4.393
IPC_protein 4.24
Toseland 4.075
ProMoST 4.368
Dawson 4.202
Bjellqvist 4.355
Wikipedia 4.101
Rodwell 4.075
Grimsley 3.986
Solomon 4.19
Lehninger 4.139
Nozaki 4.317
DTASelect 4.469
Thurlkill 4.101
EMBOSS 4.113
Sillero 4.355
Patrickios 3.63
IPC_peptide 4.19
IPC2_peptide 4.342
IPC2.peptide.svr19 4.267
Protein with the highest isoelectric point:
>tr|A0A4D6ADM4|A0A4D6ADM4_9CAUD Uncharacterized protein OS=Streptococcus phage Javan47 OX=2548188 GN=Javan47_0047 PE=4 SV=1
MM1 pKa = 7.78 RR2 pKa = 11.84 GLDD5 pKa = 3.09 RR6 pKa = 11.84 FLRR9 pKa = 11.84 SVEE12 pKa = 4.11 RR13 pKa = 11.84 KK14 pKa = 9.2 QKK16 pKa = 9.01 SARR19 pKa = 11.84 IAVDD23 pKa = 3.36 KK24 pKa = 10.62 EE25 pKa = 4.09 LSKK28 pKa = 10.9 SAARR32 pKa = 11.84 IEE34 pKa = 4.15 RR35 pKa = 11.84 QAKK38 pKa = 9.19 ILAPVDD44 pKa = 4.04 TTWLRR49 pKa = 11.84 SQIYY53 pKa = 10.27 NEE55 pKa = 4.04 QQKK58 pKa = 10.03 LLHH61 pKa = 5.87 YY62 pKa = 9.79 RR63 pKa = 11.84 VVSPALYY70 pKa = 10.27 SIYY73 pKa = 10.79 LEE75 pKa = 4.28 LGTRR79 pKa = 11.84 KK80 pKa = 8.87 MEE82 pKa = 4.32 AQPFLDD88 pKa = 3.7 PALRR92 pKa = 11.84 KK93 pKa = 8.48 EE94 pKa = 4.46 WPVLMANLKK103 pKa = 10.77 KK104 pKa = 9.96 MFKK107 pKa = 10.29 RR108 pKa = 3.74
Molecular weight: 12.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.271
IPC2_protein 9.706
IPC_protein 10.145
Toseland 10.657
ProMoST 10.379
Dawson 10.76
Bjellqvist 10.394
Wikipedia 10.906
Rodwell 11.169
Grimsley 10.804
Solomon 10.818
Lehninger 10.804
Nozaki 10.613
DTASelect 10.394
Thurlkill 10.643
EMBOSS 11.038
Sillero 10.672
Patrickios 10.906
IPC_peptide 10.833
IPC2_peptide 8.96
IPC2.peptide.svr19 8.754
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
55
0
55
12306
38
1408
223.7
25.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.525 ± 0.828
0.496 ± 0.108
6.208 ± 0.506
6.988 ± 0.633
3.608 ± 0.169
5.924 ± 0.396
1.154 ± 0.144
7.728 ± 0.393
8.207 ± 0.588
8.216 ± 0.283
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.405 ± 0.266
6.355 ± 0.4
2.82 ± 0.26
4.567 ± 0.268
4.136 ± 0.378
6.55 ± 0.705
6.363 ± 0.343
6.013 ± 0.248
1.243 ± 0.158
3.494 ± 0.357
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here