Lake Sarah-associated circular virus-20

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126G9P4|A0A126G9P4_9VIRU Coat protein OS=Lake Sarah-associated circular virus-20 OX=1685747 PE=4 SV=1
MM1 pKa = 7.81ADD3 pKa = 3.71KK4 pKa = 9.76STRR7 pKa = 11.84WAFTAYY13 pKa = 7.01EE14 pKa = 4.08TQYY17 pKa = 10.89PILDD21 pKa = 4.1ALHH24 pKa = 6.19KK25 pKa = 10.53QSSEE29 pKa = 4.21LIAEE33 pKa = 4.61IGWQDD38 pKa = 3.98EE39 pKa = 4.5ICPKK43 pKa = 9.34TGKK46 pKa = 8.8HH47 pKa = 5.3HH48 pKa = 5.88RR49 pKa = 11.84QGYY52 pKa = 8.0VRR54 pKa = 11.84TVRR57 pKa = 11.84QVRR60 pKa = 11.84FSQLRR65 pKa = 11.84EE66 pKa = 3.79IMPDD70 pKa = 2.64IHH72 pKa = 8.45LEE74 pKa = 3.96KK75 pKa = 10.6AKK77 pKa = 10.64NWEE80 pKa = 3.82ALINYY85 pKa = 6.83CQKK88 pKa = 10.79SATRR92 pKa = 11.84DD93 pKa = 3.23LSGAMVSAKK102 pKa = 9.91FEE104 pKa = 4.21RR105 pKa = 11.84PLRR108 pKa = 11.84LHH110 pKa = 6.85EE111 pKa = 4.06MLIDD115 pKa = 4.02VAHH118 pKa = 7.17HH119 pKa = 6.99IYY121 pKa = 10.51LSHH124 pKa = 7.65RR125 pKa = 11.84PGTKK129 pKa = 9.64HH130 pKa = 6.38AVCLDD135 pKa = 3.5RR136 pKa = 11.84QTDD139 pKa = 3.82RR140 pKa = 11.84QILLSYY146 pKa = 9.94LRR148 pKa = 11.84EE149 pKa = 3.8YY150 pKa = 10.96SAALVSAHH158 pKa = 6.93PEE160 pKa = 3.92YY161 pKa = 11.09AVVLVRR167 pKa = 11.84QDD169 pKa = 5.8ARR171 pKa = 11.84DD172 pKa = 3.16AWCNYY177 pKa = 8.85IEE179 pKa = 4.32VWMHH183 pKa = 5.9KK184 pKa = 10.43AEE186 pKa = 3.96QRR188 pKa = 11.84EE189 pKa = 4.25EE190 pKa = 4.26GDD192 pKa = 3.69EE193 pKa = 4.21VQQ195 pKa = 4.91

Molecular weight: 22.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126G9P4|A0A126G9P4_9VIRU Coat protein OS=Lake Sarah-associated circular virus-20 OX=1685747 PE=4 SV=1
MM1 pKa = 7.29YY2 pKa = 10.63KK3 pKa = 9.65MVMAKK8 pKa = 10.02RR9 pKa = 11.84AFKK12 pKa = 10.15PRR14 pKa = 11.84RR15 pKa = 11.84QPRR18 pKa = 11.84RR19 pKa = 11.84VPRR22 pKa = 11.84APRR25 pKa = 11.84LRR27 pKa = 11.84RR28 pKa = 11.84ARR30 pKa = 11.84PTVEE34 pKa = 3.82FASAKK39 pKa = 7.42QTIALAQDD47 pKa = 3.04AMNQVFRR54 pKa = 11.84LDD56 pKa = 4.09NIALAQFDD64 pKa = 4.03RR65 pKa = 11.84MVNIAKK71 pKa = 9.84CYY73 pKa = 9.79QYY75 pKa = 11.41FRR77 pKa = 11.84MTKK80 pKa = 9.73VEE82 pKa = 4.76LKK84 pKa = 8.51FTPQMDD90 pKa = 3.71TFTDD94 pKa = 3.64SNLQSVPYY102 pKa = 10.05LYY104 pKa = 11.28YY105 pKa = 11.01LITKK109 pKa = 9.64GDD111 pKa = 3.8NLDD114 pKa = 3.8AGTFNKK120 pKa = 10.13LRR122 pKa = 11.84DD123 pKa = 3.74AGAKK127 pKa = 10.07GIRR130 pKa = 11.84FDD132 pKa = 4.79DD133 pKa = 4.17KK134 pKa = 11.26SINVNWRR141 pKa = 11.84PAVLRR146 pKa = 11.84GVIGEE151 pKa = 4.23DD152 pKa = 2.97TNAPVNPAFTAWAEE166 pKa = 4.28AKK168 pKa = 9.05TSPWLATSYY177 pKa = 11.0LPAAQSIVWSPSAVPHH193 pKa = 6.25KK194 pKa = 10.51GILYY198 pKa = 10.18GVEE201 pKa = 3.89QDD203 pKa = 3.72SSIDD207 pKa = 3.38TKK209 pKa = 11.42YY210 pKa = 11.04YY211 pKa = 11.07ALTLTAHH218 pKa = 6.34FQFKK222 pKa = 10.45KK223 pKa = 8.73PQTYY227 pKa = 8.15STTGVIDD234 pKa = 3.86PGATVKK240 pKa = 10.48QVIDD244 pKa = 3.49KK245 pKa = 10.73GDD247 pKa = 3.71VVEE250 pKa = 5.75LPLKK254 pKa = 10.01MLEE257 pKa = 4.05

Molecular weight: 29.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

452

195

257

226.0

25.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.398 ± 0.427

1.106 ± 0.617

5.973 ± 0.217

5.088 ± 2.034

3.319 ± 1.162

3.761 ± 0.446

2.876 ± 1.804

5.088 ± 0.361

6.416 ± 0.84

7.965 ± 0.492

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.876 ± 0.204

2.876 ± 0.873

5.088 ± 1.313

5.531 ± 0.406

7.301 ± 0.59

5.088 ± 0.361

5.973 ± 1.221

6.858 ± 0.46

1.991 ± 0.374

4.425 ± 0.124
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski