Amino acid dipepetide frequency for Escherichia phage ECA2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.57AlaAla: 11.57 ± 1.485
0.614AlaCys: 0.614 ± 0.281
5.347AlaAsp: 5.347 ± 0.501
5.873AlaGlu: 5.873 ± 0.864
3.506AlaPhe: 3.506 ± 0.576
7.1AlaGly: 7.1 ± 1.008
1.841AlaHis: 1.841 ± 0.344
5.61AlaIle: 5.61 ± 0.647
6.311AlaLys: 6.311 ± 0.91
7.713AlaLeu: 7.713 ± 0.715
3.243AlaMet: 3.243 ± 0.514
3.857AlaAsn: 3.857 ± 0.57
2.279AlaPro: 2.279 ± 0.345
3.769AlaGln: 3.769 ± 0.557
5.434AlaArg: 5.434 ± 0.863
5.084AlaSer: 5.084 ± 0.857
3.769AlaThr: 3.769 ± 0.683
6.136AlaVal: 6.136 ± 0.68
1.753AlaTrp: 1.753 ± 0.406
2.892AlaTyr: 2.892 ± 0.521
0.0AlaXaa: 0.0 ± 0.0
Cys
0.701CysAla: 0.701 ± 0.289
0.088CysCys: 0.088 ± 0.096
0.789CysAsp: 0.789 ± 0.312
0.263CysGlu: 0.263 ± 0.151
0.438CysPhe: 0.438 ± 0.267
0.964CysGly: 0.964 ± 0.345
0.438CysHis: 0.438 ± 0.222
0.701CysIle: 0.701 ± 0.273
0.789CysLys: 0.789 ± 0.294
0.701CysLeu: 0.701 ± 0.253
0.088CysMet: 0.088 ± 0.085
0.175CysAsn: 0.175 ± 0.113
0.263CysPro: 0.263 ± 0.145
0.614CysGln: 0.614 ± 0.251
0.614CysArg: 0.614 ± 0.327
0.526CysSer: 0.526 ± 0.26
0.175CysThr: 0.175 ± 0.119
0.263CysVal: 0.263 ± 0.137
0.175CysTrp: 0.175 ± 0.144
0.438CysTyr: 0.438 ± 0.216
0.0CysXaa: 0.0 ± 0.0
Asp
6.311AspAla: 6.311 ± 0.64
0.526AspCys: 0.526 ± 0.199
4.47AspAsp: 4.47 ± 0.507
4.908AspGlu: 4.908 ± 0.613
2.98AspPhe: 2.98 ± 0.595
5.96AspGly: 5.96 ± 0.812
1.139AspHis: 1.139 ± 0.273
2.717AspIle: 2.717 ± 0.509
4.47AspLys: 4.47 ± 0.736
4.12AspLeu: 4.12 ± 0.739
2.63AspMet: 2.63 ± 0.516
2.892AspAsn: 2.892 ± 0.46
3.068AspPro: 3.068 ± 0.478
1.841AspGln: 1.841 ± 0.562
2.542AspArg: 2.542 ± 0.436
3.068AspSer: 3.068 ± 0.485
4.12AspThr: 4.12 ± 0.527
4.383AspVal: 4.383 ± 0.511
0.877AspTrp: 0.877 ± 0.295
1.928AspTyr: 1.928 ± 0.367
0.0AspXaa: 0.0 ± 0.0
Glu
7.538GluAla: 7.538 ± 1.029
0.789GluCys: 0.789 ± 0.25
4.996GluAsp: 4.996 ± 0.731
5.259GluGlu: 5.259 ± 0.984
2.717GluPhe: 2.717 ± 0.399
4.645GluGly: 4.645 ± 0.562
2.191GluHis: 2.191 ± 0.504
3.594GluIle: 3.594 ± 0.55
4.032GluLys: 4.032 ± 0.791
5.873GluLeu: 5.873 ± 0.643
2.892GluMet: 2.892 ± 0.472
2.367GluAsn: 2.367 ± 0.398
1.753GluPro: 1.753 ± 0.434
3.594GluGln: 3.594 ± 0.74
4.032GluArg: 4.032 ± 0.478
4.12GluSer: 4.12 ± 0.509
3.944GluThr: 3.944 ± 0.639
3.594GluVal: 3.594 ± 0.41
1.227GluTrp: 1.227 ± 0.34
3.068GluTyr: 3.068 ± 0.435
0.0GluXaa: 0.0 ± 0.0
Phe
2.454PheAla: 2.454 ± 0.509
0.351PheCys: 0.351 ± 0.199
2.98PheAsp: 2.98 ± 0.521
2.016PheGlu: 2.016 ± 0.383
1.052PhePhe: 1.052 ± 0.295
2.892PheGly: 2.892 ± 0.591
0.614PheHis: 0.614 ± 0.254
1.928PheIle: 1.928 ± 0.488
3.068PheLys: 3.068 ± 0.638
2.892PheLeu: 2.892 ± 0.586
1.315PheMet: 1.315 ± 0.333
1.928PheAsn: 1.928 ± 0.37
1.315PhePro: 1.315 ± 0.389
1.315PheGln: 1.315 ± 0.332
1.753PheArg: 1.753 ± 0.325
2.191PheSer: 2.191 ± 0.39
3.155PheThr: 3.155 ± 0.601
2.191PheVal: 2.191 ± 0.363
0.263PheTrp: 0.263 ± 0.149
0.877PheTyr: 0.877 ± 0.271
0.0PheXaa: 0.0 ± 0.0
Gly
6.223GlyAla: 6.223 ± 0.96
0.614GlyCys: 0.614 ± 0.282
5.522GlyAsp: 5.522 ± 0.836
5.522GlyGlu: 5.522 ± 0.732
3.331GlyPhe: 3.331 ± 0.439
4.645GlyGly: 4.645 ± 0.823
0.877GlyHis: 0.877 ± 0.258
5.522GlyIle: 5.522 ± 0.708
5.697GlyLys: 5.697 ± 0.776
6.136GlyLeu: 6.136 ± 0.846
2.454GlyMet: 2.454 ± 0.416
3.418GlyAsn: 3.418 ± 0.542
0.877GlyPro: 0.877 ± 0.265
2.454GlyGln: 2.454 ± 0.522
4.47GlyArg: 4.47 ± 0.471
4.207GlySer: 4.207 ± 0.536
3.155GlyThr: 3.155 ± 0.789
4.383GlyVal: 4.383 ± 0.497
1.402GlyTrp: 1.402 ± 0.392
2.717GlyTyr: 2.717 ± 0.521
0.0GlyXaa: 0.0 ± 0.0
His
1.665HisAla: 1.665 ± 0.402
0.351HisCys: 0.351 ± 0.177
0.789HisAsp: 0.789 ± 0.255
1.139HisGlu: 1.139 ± 0.417
0.877HisPhe: 0.877 ± 0.241
1.227HisGly: 1.227 ± 0.303
0.701HisHis: 0.701 ± 0.262
1.227HisIle: 1.227 ± 0.292
1.315HisLys: 1.315 ± 0.361
2.367HisLeu: 2.367 ± 0.474
0.351HisMet: 0.351 ± 0.16
1.052HisAsn: 1.052 ± 0.278
0.701HisPro: 0.701 ± 0.296
0.0HisGln: 0.0 ± 0.0
0.614HisArg: 0.614 ± 0.183
1.49HisSer: 1.49 ± 0.443
0.789HisThr: 0.789 ± 0.197
1.402HisVal: 1.402 ± 0.274
0.526HisTrp: 0.526 ± 0.196
0.877HisTyr: 0.877 ± 0.204
0.0HisXaa: 0.0 ± 0.0
Ile
4.032IleAla: 4.032 ± 0.529
0.351IleCys: 0.351 ± 0.165
3.331IleAsp: 3.331 ± 0.621
4.47IleGlu: 4.47 ± 0.784
1.315IlePhe: 1.315 ± 0.343
3.331IleGly: 3.331 ± 0.58
1.49IleHis: 1.49 ± 0.282
2.892IleIle: 2.892 ± 0.565
4.032IleLys: 4.032 ± 0.617
4.12IleLeu: 4.12 ± 0.509
0.789IleMet: 0.789 ± 0.29
2.892IleAsn: 2.892 ± 0.685
2.542IlePro: 2.542 ± 0.47
2.016IleGln: 2.016 ± 0.501
3.418IleArg: 3.418 ± 0.579
3.418IleSer: 3.418 ± 0.579
2.717IleThr: 2.717 ± 0.488
3.068IleVal: 3.068 ± 0.529
0.438IleTrp: 0.438 ± 0.229
1.928IleTyr: 1.928 ± 0.291
0.0IleXaa: 0.0 ± 0.0
Lys
7.976LysAla: 7.976 ± 1.049
0.351LysCys: 0.351 ± 0.171
3.857LysAsp: 3.857 ± 0.502
5.084LysGlu: 5.084 ± 0.539
2.542LysPhe: 2.542 ± 0.49
5.434LysGly: 5.434 ± 0.646
1.402LysHis: 1.402 ± 0.355
1.928LysIle: 1.928 ± 0.293
4.383LysLys: 4.383 ± 1.067
5.434LysLeu: 5.434 ± 1.053
1.753LysMet: 1.753 ± 0.408
2.016LysAsn: 2.016 ± 0.381
2.805LysPro: 2.805 ± 0.643
2.805LysGln: 2.805 ± 0.577
4.295LysArg: 4.295 ± 0.673
3.418LysSer: 3.418 ± 0.667
3.594LysThr: 3.594 ± 0.794
5.347LysVal: 5.347 ± 0.592
0.789LysTrp: 0.789 ± 0.254
2.016LysTyr: 2.016 ± 0.367
0.0LysXaa: 0.0 ± 0.0
Leu
6.924LeuAla: 6.924 ± 1.345
0.351LeuCys: 0.351 ± 0.19
4.996LeuAsp: 4.996 ± 0.451
6.924LeuGlu: 6.924 ± 0.984
2.542LeuPhe: 2.542 ± 0.47
4.645LeuGly: 4.645 ± 0.484
1.052LeuHis: 1.052 ± 0.256
4.12LeuIle: 4.12 ± 0.545
6.661LeuLys: 6.661 ± 0.877
4.996LeuLeu: 4.996 ± 0.804
2.279LeuMet: 2.279 ± 0.49
4.47LeuAsn: 4.47 ± 0.752
3.331LeuPro: 3.331 ± 0.524
3.769LeuGln: 3.769 ± 0.536
5.259LeuArg: 5.259 ± 0.613
4.558LeuSer: 4.558 ± 0.668
5.084LeuThr: 5.084 ± 0.523
3.944LeuVal: 3.944 ± 0.571
1.402LeuTrp: 1.402 ± 0.421
2.367LeuTyr: 2.367 ± 0.442
0.0LeuXaa: 0.0 ± 0.0
Met
3.506MetAla: 3.506 ± 0.533
0.263MetCys: 0.263 ± 0.191
1.928MetAsp: 1.928 ± 0.55
1.665MetGlu: 1.665 ± 0.543
1.052MetPhe: 1.052 ± 0.322
1.841MetGly: 1.841 ± 0.501
0.526MetHis: 0.526 ± 0.246
1.578MetIle: 1.578 ± 0.302
1.052MetLys: 1.052 ± 0.316
3.068MetLeu: 3.068 ± 0.584
1.315MetMet: 1.315 ± 0.423
1.315MetAsn: 1.315 ± 0.307
1.402MetPro: 1.402 ± 0.341
1.139MetGln: 1.139 ± 0.347
1.402MetArg: 1.402 ± 0.333
1.49MetSer: 1.49 ± 0.335
2.016MetThr: 2.016 ± 0.425
2.191MetVal: 2.191 ± 0.375
0.175MetTrp: 0.175 ± 0.177
0.614MetTyr: 0.614 ± 0.229
0.0MetXaa: 0.0 ± 0.0
Asn
4.645AsnAla: 4.645 ± 0.892
0.438AsnCys: 0.438 ± 0.257
2.805AsnAsp: 2.805 ± 0.594
2.892AsnGlu: 2.892 ± 0.455
1.402AsnPhe: 1.402 ± 0.398
4.12AsnGly: 4.12 ± 0.56
0.438AsnHis: 0.438 ± 0.176
3.068AsnIle: 3.068 ± 0.595
2.542AsnLys: 2.542 ± 0.388
2.98AsnLeu: 2.98 ± 0.411
1.052AsnMet: 1.052 ± 0.302
2.279AsnAsn: 2.279 ± 0.395
2.805AsnPro: 2.805 ± 0.542
1.841AsnGln: 1.841 ± 0.366
2.892AsnArg: 2.892 ± 0.694
2.104AsnSer: 2.104 ± 0.58
2.104AsnThr: 2.104 ± 0.423
2.104AsnVal: 2.104 ± 0.596
0.789AsnTrp: 0.789 ± 0.272
2.016AsnTyr: 2.016 ± 0.442
0.0AsnXaa: 0.0 ± 0.0
Pro
2.367ProAla: 2.367 ± 0.395
0.438ProCys: 0.438 ± 0.179
2.717ProAsp: 2.717 ± 0.482
3.331ProGlu: 3.331 ± 0.781
1.139ProPhe: 1.139 ± 0.314
1.753ProGly: 1.753 ± 0.371
0.438ProHis: 0.438 ± 0.16
1.402ProIle: 1.402 ± 0.418
2.717ProLys: 2.717 ± 0.554
3.068ProLeu: 3.068 ± 0.585
1.052ProMet: 1.052 ± 0.277
2.367ProAsn: 2.367 ± 0.466
0.964ProPro: 0.964 ± 0.376
1.139ProGln: 1.139 ± 0.366
2.016ProArg: 2.016 ± 0.422
2.016ProSer: 2.016 ± 0.34
1.665ProThr: 1.665 ± 0.359
2.367ProVal: 2.367 ± 0.477
0.701ProTrp: 0.701 ± 0.145
1.578ProTyr: 1.578 ± 0.437
0.0ProXaa: 0.0 ± 0.0
Gln
4.207GlnAla: 4.207 ± 0.685
0.263GlnCys: 0.263 ± 0.16
2.104GlnAsp: 2.104 ± 0.464
3.418GlnGlu: 3.418 ± 0.468
1.753GlnPhe: 1.753 ± 0.382
2.63GlnGly: 2.63 ± 0.392
0.614GlnHis: 0.614 ± 0.204
1.578GlnIle: 1.578 ± 0.414
2.367GlnLys: 2.367 ± 0.483
3.681GlnLeu: 3.681 ± 0.536
0.701GlnMet: 0.701 ± 0.211
1.578GlnAsn: 1.578 ± 0.368
1.578GlnPro: 1.578 ± 0.457
1.841GlnGln: 1.841 ± 0.371
2.016GlnArg: 2.016 ± 0.407
1.841GlnSer: 1.841 ± 0.461
2.279GlnThr: 2.279 ± 0.558
2.805GlnVal: 2.805 ± 0.391
0.877GlnTrp: 0.877 ± 0.245
1.402GlnTyr: 1.402 ± 0.416
0.0GlnXaa: 0.0 ± 0.0
Arg
5.61ArgAla: 5.61 ± 0.606
0.877ArgCys: 0.877 ± 0.273
3.769ArgAsp: 3.769 ± 0.695
4.032ArgGlu: 4.032 ± 0.682
2.98ArgPhe: 2.98 ± 0.442
3.857ArgGly: 3.857 ± 0.607
1.315ArgHis: 1.315 ± 0.371
2.98ArgIle: 2.98 ± 0.568
3.857ArgLys: 3.857 ± 0.63
5.259ArgLeu: 5.259 ± 0.616
1.665ArgMet: 1.665 ± 0.537
2.454ArgAsn: 2.454 ± 0.499
1.315ArgPro: 1.315 ± 0.294
1.928ArgGln: 1.928 ± 0.4
3.068ArgArg: 3.068 ± 0.564
4.383ArgSer: 4.383 ± 0.735
2.542ArgThr: 2.542 ± 0.414
3.155ArgVal: 3.155 ± 0.447
0.789ArgTrp: 0.789 ± 0.279
1.139ArgTyr: 1.139 ± 0.21
0.0ArgXaa: 0.0 ± 0.0
Ser
4.821SerAla: 4.821 ± 0.474
0.614SerCys: 0.614 ± 0.221
5.259SerAsp: 5.259 ± 0.773
3.944SerGlu: 3.944 ± 0.909
2.63SerPhe: 2.63 ± 0.538
5.171SerGly: 5.171 ± 0.616
1.052SerHis: 1.052 ± 0.261
3.068SerIle: 3.068 ± 0.688
3.155SerLys: 3.155 ± 0.607
3.418SerLeu: 3.418 ± 0.513
1.227SerMet: 1.227 ± 0.427
1.928SerAsn: 1.928 ± 0.473
2.104SerPro: 2.104 ± 0.534
2.191SerGln: 2.191 ± 0.495
3.068SerArg: 3.068 ± 0.456
3.594SerSer: 3.594 ± 0.753
3.681SerThr: 3.681 ± 0.512
4.032SerVal: 4.032 ± 0.588
0.526SerTrp: 0.526 ± 0.121
2.367SerTyr: 2.367 ± 0.58
0.0SerXaa: 0.0 ± 0.0
Thr
4.383ThrAla: 4.383 ± 0.811
0.789ThrCys: 0.789 ± 0.35
2.63ThrAsp: 2.63 ± 0.552
3.506ThrGlu: 3.506 ± 0.595
1.665ThrPhe: 1.665 ± 0.397
4.821ThrGly: 4.821 ± 0.79
1.402ThrHis: 1.402 ± 0.233
3.418ThrIle: 3.418 ± 0.6
3.944ThrLys: 3.944 ± 0.465
4.908ThrLeu: 4.908 ± 0.771
1.139ThrMet: 1.139 ± 0.366
2.454ThrAsn: 2.454 ± 0.978
2.63ThrPro: 2.63 ± 0.446
2.016ThrGln: 2.016 ± 0.366
2.191ThrArg: 2.191 ± 0.321
4.383ThrSer: 4.383 ± 0.975
3.155ThrThr: 3.155 ± 0.56
3.857ThrVal: 3.857 ± 0.677
0.351ThrTrp: 0.351 ± 0.152
1.841ThrTyr: 1.841 ± 0.361
0.0ThrXaa: 0.0 ± 0.0
Val
4.733ValAla: 4.733 ± 0.531
0.438ValCys: 0.438 ± 0.198
3.506ValAsp: 3.506 ± 0.439
4.645ValGlu: 4.645 ± 0.637
1.49ValPhe: 1.49 ± 0.443
4.996ValGly: 4.996 ± 0.831
0.964ValHis: 0.964 ± 0.305
2.805ValIle: 2.805 ± 0.444
4.032ValLys: 4.032 ± 0.602
4.558ValLeu: 4.558 ± 0.652
1.665ValMet: 1.665 ± 0.369
3.418ValAsn: 3.418 ± 0.608
2.279ValPro: 2.279 ± 0.468
2.98ValGln: 2.98 ± 0.54
4.558ValArg: 4.558 ± 0.541
3.331ValSer: 3.331 ± 0.468
4.908ValThr: 4.908 ± 0.549
4.821ValVal: 4.821 ± 0.754
0.877ValTrp: 0.877 ± 0.373
2.104ValTyr: 2.104 ± 0.441
0.0ValXaa: 0.0 ± 0.0
Trp
0.964TrpAla: 0.964 ± 0.219
0.438TrpCys: 0.438 ± 0.22
0.701TrpAsp: 0.701 ± 0.248
0.877TrpGlu: 0.877 ± 0.239
0.263TrpPhe: 0.263 ± 0.173
1.139TrpGly: 1.139 ± 0.288
0.263TrpHis: 0.263 ± 0.134
0.526TrpIle: 0.526 ± 0.306
1.052TrpLys: 1.052 ± 0.364
1.665TrpLeu: 1.665 ± 0.423
0.614TrpMet: 0.614 ± 0.232
1.052TrpAsn: 1.052 ± 0.255
0.263TrpPro: 0.263 ± 0.144
0.789TrpGln: 0.789 ± 0.256
0.964TrpArg: 0.964 ± 0.256
0.789TrpSer: 0.789 ± 0.353
0.877TrpThr: 0.877 ± 0.282
1.139TrpVal: 1.139 ± 0.465
0.351TrpTrp: 0.351 ± 0.147
0.175TrpTyr: 0.175 ± 0.125
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.068TyrAla: 3.068 ± 0.491
0.263TyrCys: 0.263 ± 0.145
2.454TyrAsp: 2.454 ± 0.649
2.454TyrGlu: 2.454 ± 0.472
0.877TyrPhe: 0.877 ± 0.344
2.454TyrGly: 2.454 ± 0.474
0.701TyrHis: 0.701 ± 0.227
1.928TyrIle: 1.928 ± 0.541
1.753TyrLys: 1.753 ± 0.315
2.717TyrLeu: 2.717 ± 0.515
1.227TyrMet: 1.227 ± 0.296
1.402TyrAsn: 1.402 ± 0.288
0.964TyrPro: 0.964 ± 0.274
1.49TyrGln: 1.49 ± 0.457
2.367TyrArg: 2.367 ± 0.379
1.841TyrSer: 1.841 ± 0.401
1.841TyrThr: 1.841 ± 0.52
1.928TyrVal: 1.928 ± 0.383
0.614TyrTrp: 0.614 ± 0.288
0.526TyrTyr: 0.526 ± 0.198
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 47 proteins (11410 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski