Lewinella marina

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Saprospiria; Saprospirales; Lewinellaceae; Lewinella

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3525 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2G0CBM1|A0A2G0CBM1_9BACT Phosphoribosylformylglycinamidine synthase subunit PurQ OS=Lewinella marina OX=438751 GN=purQ PE=3 SV=1
MM1 pKa = 7.38KK2 pKa = 9.41PDD4 pKa = 3.44KK5 pKa = 8.84PTLVGSSSRR14 pKa = 11.84RR15 pKa = 11.84PLPAAALRR23 pKa = 11.84MLMNYY28 pKa = 8.03TFMPDD33 pKa = 3.03RR34 pKa = 11.84PGIRR38 pKa = 11.84ALFLLLFAIFGVTGLRR54 pKa = 11.84AQLALTVTKK63 pKa = 10.45TDD65 pKa = 3.41ISCLGEE71 pKa = 3.82RR72 pKa = 11.84DD73 pKa = 3.62GVITVSARR81 pKa = 11.84GGSPDD86 pKa = 3.43YY87 pKa = 10.93DD88 pKa = 3.73FSIVRR93 pKa = 11.84TRR95 pKa = 11.84PATPPVTGSNSTNSDD110 pKa = 2.58GVAINFTNLNSGEE123 pKa = 4.19YY124 pKa = 9.3TVTVTDD130 pKa = 3.74AAGNTATDD138 pKa = 3.38QVEE141 pKa = 4.05ILEE144 pKa = 4.42PNITTAIQVVQEE156 pKa = 4.09IQCFDD161 pKa = 3.63DD162 pKa = 3.58TNGILEE168 pKa = 4.19AVVFDD173 pKa = 4.03GSNPISDD180 pKa = 3.52YY181 pKa = 11.57SNYY184 pKa = 7.8TFRR187 pKa = 11.84WSTGAITRR195 pKa = 11.84RR196 pKa = 11.84ISALGPDD203 pKa = 3.86QYY205 pKa = 11.75SVTVTNTTTGCASSDD220 pKa = 3.85SEE222 pKa = 4.45TLRR225 pKa = 11.84SPSRR229 pKa = 11.84LVANSSQLRR238 pKa = 11.84SDD240 pKa = 3.66AATCSGVSDD249 pKa = 3.69GTVNVPITGGTKK261 pKa = 8.49NTNGNYY267 pKa = 9.93RR268 pKa = 11.84INWSDD273 pKa = 3.23GTEE276 pKa = 4.0TLDD279 pKa = 3.91NKK281 pKa = 10.13IARR284 pKa = 11.84SDD286 pKa = 4.15LEE288 pKa = 4.47PGSYY292 pKa = 9.06TVTVTDD298 pKa = 4.0ARR300 pKa = 11.84SCEE303 pKa = 4.21TTTSFTVAAEE313 pKa = 4.01KK314 pKa = 10.15TLLLTAATTDD324 pKa = 3.51ISCFGDD330 pKa = 3.3ANGSISVTAGFEE342 pKa = 4.24GATPDD347 pKa = 3.56YY348 pKa = 10.7PFEE351 pKa = 4.24TRR353 pKa = 11.84LLAEE357 pKa = 5.6DD358 pKa = 3.55GTEE361 pKa = 3.77LVGYY365 pKa = 7.38QTVANQGRR373 pKa = 11.84TPRR376 pKa = 11.84VFDD379 pKa = 3.42NLGPGNYY386 pKa = 9.67LVVLRR391 pKa = 11.84DD392 pKa = 3.64QDD394 pKa = 4.6SEE396 pKa = 4.76GCTVTDD402 pKa = 3.36TVTIIEE408 pKa = 4.34PALLEE413 pKa = 4.33IDD415 pKa = 3.83TVEE418 pKa = 4.31TTDD421 pKa = 4.49FGCPDD426 pKa = 3.62EE427 pKa = 5.8FGTATINFTGGTAPYY442 pKa = 9.67TFRR445 pKa = 11.84FKK447 pKa = 10.99NDD449 pKa = 3.65SLPHH453 pKa = 5.77PTDD456 pKa = 2.68TTMRR460 pKa = 11.84FDD462 pKa = 4.46TIVADD467 pKa = 4.42TNFLDD472 pKa = 4.76QLQPDD477 pKa = 4.06TNYY480 pKa = 10.41VAFVTDD486 pKa = 4.18ANGCIDD492 pKa = 3.57SLRR495 pKa = 11.84FKK497 pKa = 10.59IFSPPRR503 pKa = 11.84ASIQPIATDD512 pKa = 3.73SVSCPSSSDD521 pKa = 3.2GQLFTIVTPPQGEE534 pKa = 4.7TVTEE538 pKa = 4.22TAWYY542 pKa = 9.66RR543 pKa = 11.84INNDD547 pKa = 2.84GTIGEE552 pKa = 4.27QVDD555 pKa = 2.87IGTRR559 pKa = 11.84TTADD563 pKa = 3.51LSVGFYY569 pKa = 10.7LFEE572 pKa = 4.43ATLSNACVSQAIGEE586 pKa = 4.4VASPGLVEE594 pKa = 5.66LDD596 pKa = 4.1SFDD599 pKa = 4.13LQPPVCLGEE608 pKa = 4.42SNGSIFVYY616 pKa = 8.73PTGGTPPYY624 pKa = 10.2RR625 pKa = 11.84YY626 pKa = 9.25DD627 pKa = 3.02WSVAGASTSANFITNLSAGTYY648 pKa = 10.16SVTITDD654 pKa = 4.9ANNCQPPFSTTFVLEE669 pKa = 4.32DD670 pKa = 3.44PVGITGTFSDD680 pKa = 4.63LQGVSCPDD688 pKa = 3.17EE689 pKa = 4.29STADD693 pKa = 3.48GAATFDD699 pKa = 4.09ALFSDD704 pKa = 4.91GSTGTFDD711 pKa = 5.35FYY713 pKa = 10.72WSSGDD718 pKa = 3.69TITGQSSATVTGLTRR733 pKa = 11.84GPISVTVTDD742 pKa = 4.72GLCPQVFTDD751 pKa = 5.09TISSPEE757 pKa = 3.85DD758 pKa = 3.31FEE760 pKa = 5.16IAFATEE766 pKa = 4.18DD767 pKa = 3.88VNCFGQSDD775 pKa = 4.39GSVTAQVTGGTPGYY789 pKa = 8.45TYY791 pKa = 11.29NWIDD795 pKa = 3.66RR796 pKa = 11.84PEE798 pKa = 4.23TGPAITGVPAGSYY811 pKa = 10.2QLLITDD817 pKa = 4.83ARR819 pKa = 11.84GCQPDD824 pKa = 3.51TAAAVVNQPDD834 pKa = 4.6PLTLSINPTLTTPTVTCAGDD854 pKa = 3.64SNGVVAVFVSSTNNNPLANNPYY876 pKa = 9.01RR877 pKa = 11.84WSANVADD884 pKa = 4.76NDD886 pKa = 4.44DD887 pKa = 3.88NVAGNLAPGTYY898 pKa = 10.21AVTVTDD904 pKa = 4.64VEE906 pKa = 4.76GCQDD910 pKa = 3.36SLQYY914 pKa = 10.44TIIEE918 pKa = 4.15PQPITFAVQDD928 pKa = 4.34IEE930 pKa = 4.39PPLCFGEE937 pKa = 4.35MTTVRR942 pKa = 11.84LDD944 pKa = 3.41TAFGGQSSGFDD955 pKa = 3.31DD956 pKa = 4.93FTFTLNNDD964 pKa = 3.57GFLLPADD971 pKa = 3.87QPGSTFAGEE980 pKa = 4.3VVVTVFDD987 pKa = 4.27SVGCRR992 pKa = 11.84SDD994 pKa = 3.09LTFNVEE1000 pKa = 3.94QPEE1003 pKa = 4.41EE1004 pKa = 4.01IVIEE1008 pKa = 4.16LPEE1011 pKa = 4.75RR1012 pKa = 11.84LLVDD1016 pKa = 4.85LGDD1019 pKa = 3.89TLEE1022 pKa = 4.05QLTPVVTPMSSNYY1035 pKa = 10.29SYY1037 pKa = 11.37LWTPAEE1043 pKa = 4.23FLSADD1048 pKa = 3.5TVANPFVTPLQTRR1061 pKa = 11.84IYY1063 pKa = 8.69TLTVTDD1069 pKa = 3.68QNGCEE1074 pKa = 4.17AIEE1077 pKa = 5.05DD1078 pKa = 3.68ILVEE1082 pKa = 3.81VDD1084 pKa = 3.17ANRR1087 pKa = 11.84NVYY1090 pKa = 9.97LPNAFSPNQDD1100 pKa = 2.51GRR1102 pKa = 11.84NEE1104 pKa = 3.82EE1105 pKa = 3.92FRR1107 pKa = 11.84IYY1109 pKa = 10.7ACQGVVGVPRR1119 pKa = 11.84VNIFNRR1125 pKa = 11.84WGGLVYY1131 pKa = 10.32EE1132 pKa = 4.82ATDD1135 pKa = 3.53IPANCLDD1142 pKa = 4.64GTLLWDD1148 pKa = 3.53GRR1150 pKa = 11.84TPSGQLADD1158 pKa = 4.0LGVYY1162 pKa = 10.16VYY1164 pKa = 9.5TVEE1167 pKa = 3.92VAFIDD1172 pKa = 4.59GVTLLYY1178 pKa = 10.62RR1179 pKa = 11.84GDD1181 pKa = 3.4IAVIRR1186 pKa = 4.25

Molecular weight: 126.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2G0CDI0|A0A2G0CDI0_9BACT Carrier domain-containing protein OS=Lewinella marina OX=438751 GN=CGL56_12685 PE=4 SV=1
MM1 pKa = 7.47SSAGRR6 pKa = 11.84KK7 pKa = 8.54GGFRR11 pKa = 11.84RR12 pKa = 11.84FYY14 pKa = 8.4WLSRR18 pKa = 11.84VFPGQEE24 pKa = 3.84GQQFGAYY31 pKa = 8.71RR32 pKa = 11.84RR33 pKa = 11.84LVRR36 pKa = 11.84EE37 pKa = 3.76NNIRR41 pKa = 11.84CPQGARR47 pKa = 11.84RR48 pKa = 11.84VDD50 pKa = 3.59RR51 pKa = 11.84HH52 pKa = 5.63PMSVEE57 pKa = 3.14PRR59 pKa = 11.84EE60 pKa = 4.19FVEE63 pKa = 5.53KK64 pKa = 10.81NHH66 pKa = 6.14SPCNKK71 pKa = 9.4APRR74 pKa = 11.84LLRR77 pKa = 11.84VPDD80 pKa = 3.54KK81 pKa = 10.48RR82 pKa = 11.84TGVQSFSSLVEE93 pKa = 3.96SVRR96 pKa = 11.84DD97 pKa = 3.38RR98 pKa = 11.84LYY100 pKa = 10.81RR101 pKa = 11.84LAVRR105 pKa = 11.84MTGDD109 pKa = 3.03GSEE112 pKa = 4.61AEE114 pKa = 4.58DD115 pKa = 3.66VVQEE119 pKa = 3.99VLISGWQRR127 pKa = 11.84KK128 pKa = 7.38EE129 pKa = 4.27EE130 pKa = 3.97IVQLEE135 pKa = 4.33NPPAWLMRR143 pKa = 11.84MTHH146 pKa = 5.38NRR148 pKa = 11.84AIDD151 pKa = 3.38RR152 pKa = 11.84LRR154 pKa = 11.84SRR156 pKa = 11.84KK157 pKa = 9.52ARR159 pKa = 11.84TGYY162 pKa = 9.82EE163 pKa = 3.74RR164 pKa = 11.84AAAPADD170 pKa = 3.81YY171 pKa = 10.71HH172 pKa = 6.76GLTPLRR178 pKa = 11.84LLEE181 pKa = 4.32TRR183 pKa = 11.84DD184 pKa = 3.71TLDD187 pKa = 4.28LIHH190 pKa = 6.87RR191 pKa = 11.84QMARR195 pKa = 11.84LSPEE199 pKa = 3.69QRR201 pKa = 11.84SVLQLRR207 pKa = 11.84EE208 pKa = 4.13IEE210 pKa = 4.13GMSYY214 pKa = 10.74RR215 pKa = 11.84EE216 pKa = 3.77IAEE219 pKa = 4.02ATGLSTEE226 pKa = 4.04QVKK229 pKa = 10.46VYY231 pKa = 8.63LHH233 pKa = 6.48RR234 pKa = 11.84GRR236 pKa = 11.84RR237 pKa = 11.84QLRR240 pKa = 11.84QWLLQEE246 pKa = 4.87KK247 pKa = 9.94IVEE250 pKa = 4.21RR251 pKa = 4.29

Molecular weight: 29.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3525

0

3525

1348891

38

5003

382.7

42.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.195 ± 0.046

0.763 ± 0.014

6.094 ± 0.036

6.371 ± 0.04

4.237 ± 0.025

8.088 ± 0.037

2.084 ± 0.021

4.973 ± 0.029

3.195 ± 0.033

10.409 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.947 ± 0.018

3.787 ± 0.034

5.012 ± 0.029

3.564 ± 0.023

6.797 ± 0.043

5.522 ± 0.028

5.907 ± 0.038

6.939 ± 0.028

1.313 ± 0.016

3.801 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski